Restriction map for
pFN21ASDA0019
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# NO CUT ENZYMES ( 88 enzymes) #
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Acc65I AccI AclI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbsI BglII BlnI BpuAI BsaAI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BspMI BsrDI BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraIII Eam1105I Eco47III Eco81I FspI
HpaI KpnI KspI MfeI MluI
MunI NdeI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AatII 1474 GACGTC GACGT^C
AccII 1539 CGCG CG^CG
AccIII 2040 TCCGGA T^CCGGA
AflII 745 CTTAAG C^TTAAG
AlwI 345 GATCC GGATC(4/5)
BalI 1193 TGGCCA TGG^CCA
BanI 1218 GGCGCC G^G[CT][GA]CC
BbeI 1218 GGCGCC GGCGC^C
BbvCI 1615 GCTGAGG CCTCAGC(-5/-2)
BcgI 603 CGAGGAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1795 GTATCC GTATCC(6/5)
BclI 2165 TGATCA T^GATCA
BfrI 745 CTTAAG C^TTAAG
BlpI 1768 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1768 GCTGAGC GC^T[ACGT]AGC
BsaBI 1087 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2040 TCCGGA [AT]^CCGG[AT]
BseAI 2040 TCCGGA T^CCGGA
BsgI 83 GTGCAG GTGCAG(16/14)
BspEI 2040 TCCGGA T^CCGGA
BspHI 711 TCATGA T^CATGA
BsrBI 1212 GAGCGG CCGCTC(-3/-3)
BssSI 1234 CACGAG CACGAG(-5/-1)
BstAPI 2380 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2144 GGTCACC G^GT[ACGT]ACC
BstPI 2144 GGTCACC G^GT[ACGT]ACC
BstUI 1539 CGCG CG^CG
BstXI 1851 CCAGCTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 344 AGATCC [GA]^GATC[CT]
CelII 1768 GCTGAGC GC^T[ACGT]AGC
DraI 1813 TTTAAA TTT^AAA
DrdI 433 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 634 CTCTTC CTCTTC(1/4)
EcoNI 978 CCTGGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2144 GGTCACC G^GT[ACGT]ACC
EcoRI 3 GAATTC G^AATTC
EcoT22I 658 ATGCAT ATGCA^T
FbaI 2165 TGATCA T^GATCA
FseI 1177 GGCCGGCC GGCCGG^CC
KasI 1218 GGCGCC G^GCGCC
Ksp632I 634 CTCTTC CTCTTC(1/4)
MamI 1087 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 344 AGATCC [GA]^GATC[CT]
MluNI 1193 TGGCCA TGG^CCA
MroI 2040 TCCGGA T^CCGGA
MscI 1193 TGGCCA TGG^CCA
MslI 591 CATGTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1539 CGCG CG^CG
NaeI 1178 GCCGGC GCC^GGC
NarI 1218 GGCGCC GG^CGCC
NgoMIV 1178 GCCGGC G^CCGGC
NsiI 658 ATGCAT ATGCA^T
PstI 2333 CTGCAG CTGCA^G
PvuII 1400 CAGCTG CAG^CTG
RcaI 711 TCATGA T^CATGA
SacI 163 GAGCTC GAGCT^C
SexAI 1074 ACCTGGT A^CC[AT]GGT
SfoI 1218 GGCGCC GGC^GCC
SphI 1734 GCATGC GCATG^C
XcmI 1499 CCAAATACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 344 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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BglI 613 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1104 GCCCTTGGGGC
BpmI 307 CTCCAG CTGGAG(16/14)
2426 CTCCAG
BsaI 973 GAGACC GGTCTC(1/5)
2004 GAGACC
BsiEI 1180 CGGCCG CG[GA][CT]^CG
2375 CGGCCG
BsmBI 1476 CGTCTC CGTCTC(1/5)
2236 GAGACG
BsrFI 1178 GCCGGC [GA]^CCGG[CT]
2373 ACCGGC
Cfr10I 1178 GCCGGC [GA]^CCGG[CT]
2373 ACCGGC
EagI 1180 CGGCCG C^GGCCG
2375 CGGCCG
EclXI 1180 CGGCCG C^GGCCG
2375 CGGCCG
Eco52I 1180 CGGCCG C^GGCCG
2375 CGGCCG
HincII 442 GTCAAC GT[CT]^[GA]AC
2107 GTTGAC
HindII 442 GTCAAC GT[CT]^[GA]AC
2107 GTTGAC
NheI 1875 GCTAGC G^CTAGC
2266 GCTAGC
NspI 651 ACATGC [GA]CATG^[CT]
1734 GCATGC
PflMI 1069 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2149 CCAAAGAATGG
PleI 1983 GACTC GAGTC(4/5)
2470 GAGTC
PpuMI 371 GGGACCT [GA]G^G[AT]CC[CT]
1560 AGGTCCT
TaqI 1524 TCGA T^CGA
2088 TCGA
Tsp45I 130 GTCAC ^GT[GC]AC
2145 GTCAC
TthHB8I 1524 TCGA T^CGA
2088 TCGA
Van91I 1069 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2149 CCAAAGAATGG
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwNI ApaI ApoI Asp700I AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BmyI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp509I TspRI XmnI