(Circular) (Six-base) MAPSORT of: pFN21ASDA0019.seq Check: 4427 from: 1 to: 7213 pFN21ASDA0019 7213 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3501 5023 278 Size: 53 83 186 936 1965 1522 2468 Fragments arranged by size: 2468 1965 1522 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4556 1937 Size: 2619 4594 Acc65I G'GTAC_C Cuts at: 4534 4534 Size: 7213 AclI AA'CG_TT Cuts at: 1563 5340 5713 1563 Size: 3777 373 3063 Fragments arranged by size: 3777 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3032 3137 5276 6660 6928 1632 Size: 1400 105 2139 1384 268 1917 Fragments arranged by size: 2139 1917 1400 1384 268 105 AflII C'TTAA_G Cuts at: 820 1017 2768 820 Size: 197 1751 5265 Fragments arranged by size: 5265 1751 197 AflIII A'CryG_T Cuts at: 1203 6112 7089 1203 Size: 4909 977 1327 Fragments arranged by size: 4909 1327 977 AgeI A'CCGG_T Cuts at: 6044 6044 Size: 7213 AhdI GACnn_n'nnGTC Cuts at: 5942 5942 Size: 7213 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7213 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7213 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2889 2921 2889 Size: 32 7181 AlwNI CAG_nnn'CTG Cuts at: 3098 3587 4364 4956 6528 3098 Size: 489 777 592 1572 3783 Fragments arranged by size: 3783 1572 777 592 489 ApaI G_GGCC'C Cuts at: 2356 2647 4443 2356 Size: 291 1796 5126 Fragments arranged by size: 5126 1796 291 ApaLI G'TGCA_C Cuts at: 5270 6426 5270 Size: 1156 6057 ApoI r'AATT_y Cuts at: 1493 2026 2310 2753 2984 4522 4748 4900 7009 Size: 533 284 443 231 1538 226 152 2109 Cuts at: 7009 1493 Size: 1697 Fragments arranged by size: 2109 1697 1538 533 443 284 231 226 152 AseI AT'TA_AT Cuts at: 160 1032 5767 160 Size: 872 4735 1606 Fragments arranged by size: 4735 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7213 AvaI C'yCGr_G Cuts at: 1870 1943 3296 3778 3891 4281 4538 1870 Size: 73 1353 482 113 390 257 4545 Fragments arranged by size: 4545 1353 482 390 257 113 73 BamHI G'GATC_C Cuts at: 4543 4910 4543 Size: 367 6846 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3241 4534 5989 618 Size: 325 217 627 1454 1293 1455 1842 Fragments arranged by size: 1842 1455 1454 1293 627 325 217 BanII G_rGCy'C Cuts at: 1391 2190 2356 2647 3212 3659 3752 4443 4532 Size: 799 166 291 565 447 93 691 89 Cuts at: 4532 1391 Size: 4072 Fragments arranged by size: 4072 799 691 565 447 291 166 93 89 BbeI G_GCGC'C Cuts at: 3245 3245 Size: 7213 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6242 BbvCI CC'TCA_GC Cuts at: 3639 3639 Size: 7213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2615 2649 5404 5438 1903 Size: 34 678 34 2755 34 3678 Fragments arranged by size: 3678 2755 678 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3829 5105 6315 3829 Size: 1276 1210 4727 Fragments arranged by size: 4727 1276 1210 BclI T'GATC_A Cuts at: 1548 4188 1548 Size: 2640 4573 BfrBI ATG'CAT Cuts at: 2683 2683 Size: 7213 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2642 3133 5824 Size: 107 122 71 631 605 970 491 2691 Cuts at: 5824 136 Size: 1525 Fragments arranged by size: 2691 1525 970 631 605 491 122 107 71 BglII A'GATC_T Cuts at: 7208 7208 Size: 7213 BlpI GC'TnA_GC Cuts at: 3792 4630 3792 Size: 838 6375 Bme1580I G_kGCm'C Cuts at: 1790 2356 2647 3477 4443 5274 6430 1790 Size: 566 291 830 966 831 1156 2573 Fragments arranged by size: 2573 1156 966 831 830 566 291 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7213 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4944 5902 442 Size: 416 53 83 399 24 3527 958 1753 Fragments arranged by size: 3527 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3902 4293 1056 Size: 2846 391 3976 Fragments arranged by size: 3976 2846 391 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 4434 5873 1239 Size: 1076 2119 1439 2579 Fragments arranged by size: 2579 2119 1439 1076 Bpu10I CC'TnA_GC Cuts at: 3639 3639 Size: 7213 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2384 2737 2772 3196 5338 6203 6501 2384 Size: 353 35 424 2142 865 298 3096 Fragments arranged by size: 3096 2142 865 424 353 298 35 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2990 4021 5876 882 Size: 617 1491 1031 1855 2219 Fragments arranged by size: 2219 1855 1491 1031 617 BsaAI yAC'GTr Cuts at: 493 1204 4510 5004 493 Size: 711 3306 494 2702 Fragments arranged by size: 3306 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3114 7207 3114 Size: 4093 3120 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3165 3242 3293 3498 Size: 53 83 186 936 1632 77 51 205 Cuts at: 3498 5020 5402 6037 275 Size: 1522 382 635 1451 Fragments arranged by size: 1632 1522 1451 936 635 382 205 186 83 77 53 51 BsaWI w'CCGG_w Cuts at: 4063 5646 6044 6318 6465 6876 4063 Size: 1583 398 274 147 411 4400 Fragments arranged by size: 4400 1583 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2889 2919 3248 3278 1178 Size: 30 165 30 1486 30 329 30 5113 Fragments arranged by size: 5113 1486 329 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2195 2198 2600 3205 3347 3350 3734 1186 Size: 1009 3 402 605 142 3 384 4665 Fragments arranged by size: 4665 1009 605 402 384 142 3 3 BseYI C'CCAG_C Cuts at: 3773 3873 4142 6416 6912 3773 Size: 100 269 2274 496 4074 Fragments arranged by size: 4074 2274 496 269 100 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2127 1163 Size: 573 391 6249 Fragments arranged by size: 6249 573 391 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3206 4401 4663 5424 6452 1540 Size: 275 186 1205 1195 262 761 1028 2301 Fragments arranged by size: 2301 1205 1195 1028 761 275 262 186 BsiHKAI G_wGCw'C Cuts at: 2190 2425 3264 3307 5274 5359 6430 2190 Size: 235 839 43 1967 85 1071 2973 Fragments arranged by size: 2973 1967 1071 839 235 85 43 BsmI GAATG_Cn' Cuts at: 4724 4817 4724 Size: 93 7120 BsmBI CGTCTCn'nnnn_ Cuts at: 3505 4253 3505 Size: 748 6465 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 2356 2425 2647 3212 3264 3307 Size: 399 400 166 69 222 565 52 43 Cuts at: 3307 3477 3659 3752 4443 4532 5274 5359 6430 Size: 170 182 93 691 89 742 85 1071 Cuts at: 6430 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 691 565 400 399 222 182 170 166 93 89 85 69 52 43 BspEI T'CCGG_A Cuts at: 4063 6876 4063 Size: 2813 4400 BspHI T'CATG_A Cuts at: 2734 5102 6832 2734 Size: 2368 1730 3115 Fragments arranged by size: 3115 2368 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4568 844 Size: 3724 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3237 5100 1394 Size: 429 1414 1863 3507 Fragments arranged by size: 3507 1863 1414 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5708 5882 65 Size: 1183 4460 174 1396 Fragments arranged by size: 4460 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3201 4396 5857 6044 1700 Size: 110 1391 1195 1461 187 2869 Fragments arranged by size: 2869 1461 1391 1195 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7213 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7213 BssSI C'ACGA_G Cuts at: 3257 5273 6285 3257 Size: 2016 1012 4185 Fragments arranged by size: 4185 2016 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4409 794 Size: 3615 3598 BstBI TT'CG_AA Cuts at: 2007 6017 2007 Size: 4010 3203 BstEII G'GTnAC_C Cuts at: 1184 4167 1184 Size: 2983 4230 BstXI CCAn_nnnn'nTGG Cuts at: 3881 3881 Size: 7213 BstYI r'GATC_y Cuts at: 1972 2367 4543 4910 5295 5312 6764 6850 6862 Size: 395 2176 367 385 17 1452 86 12 Cuts at: 6862 7208 1972 Size: 346 1977 Fragments arranged by size: 2176 1977 1452 395 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2967 3531 3714 3937 4294 4345 513 Size: 552 1902 564 183 223 357 51 3381 Fragments arranged by size: 3381 1902 564 552 357 223 183 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3651 487 Size: 683 56 2425 4049 Fragments arranged by size: 4049 2425 683 56 BtsI GCAGTG_nn' Cuts at: 3660 4729 5523 5543 3660 Size: 1069 794 20 5330 Fragments arranged by size: 5330 1069 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7213 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7178 DraI TTT'AAA Cuts at: 3838 4517 3838 Size: 679 6534 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2462 6220 809 Size: 507 1146 3758 1802 Fragments arranged by size: 3758 1802 1146 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3203 3216 3614 Size: 54 1425 50 275 18 1373 13 398 Cuts at: 3614 4398 4660 5549 7178 8 Size: 784 262 889 1629 43 Fragments arranged by size: 1629 1425 1373 889 784 398 275 262 54 50 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 3203 4398 4660 1537 Size: 275 1391 1195 262 4090 Fragments arranged by size: 4090 1391 1195 275 262 EarI CTCTTCn'nnn_ Cuts at: 1353 2663 5143 1353 Size: 1310 2480 3423 Fragments arranged by size: 3423 2480 1310 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5800 6174 6320 387 Size: 1064 138 4211 374 146 1280 Fragments arranged by size: 4211 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2188 2188 Size: 7213 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 3032 3137 4434 5276 5873 6660 Size: 393 683 717 105 1297 842 597 787 Cuts at: 6660 6928 1239 Size: 268 1524 Fragments arranged by size: 1524 1297 842 787 717 683 597 393 268 105 EcoNI CCTnn'n_nnAGG Cuts at: 3005 3005 Size: 7213 EcoO109I rG'GnC_Cy Cuts at: 2353 2395 2644 2666 3584 3628 4439 4440 2353 Size: 42 249 22 918 44 811 1 5126 Fragments arranged by size: 5126 918 811 249 44 42 22 1 EcoRI G'AATT_C Cuts at: 2026 4522 4900 2026 Size: 2496 378 4339 Fragments arranged by size: 4339 2496 378 EcoRV GAT'ATC Cuts at: 2881 3866 4427 2881 Size: 985 561 5667 Fragments arranged by size: 5667 985 561 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2794 3145 2794 Size: 351 6862 FseI GG_CCGG'CC Cuts at: 3205 3205 Size: 7213 FspI TGC'GCA Cuts at: 5719 5719 Size: 7213 HaeII r_GCGC'y Cuts at: 1678 3245 3711 3728 6360 1678 Size: 1567 466 17 2632 2531 Fragments arranged by size: 2632 2531 1567 466 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2331 2363 5854 5886 5928 Size: 32 135 32 759 32 3491 32 42 Cuts at: 5928 5960 1373 Size: 32 2626 Fragments arranged by size: 3491 2626 759 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2467 4132 4557 4803 669 Size: 988 281 529 1665 425 246 3079 Fragments arranged by size: 3079 1665 988 529 425 281 246 HindIII A'AGCT_T Cuts at: 748 2765 3347 4389 748 Size: 2017 582 1042 3572 Fragments arranged by size: 3572 2017 1042 582 HpaI GTT'AAC Cuts at: 4803 4803 Size: 7213 KasI G'GCGC_C Cuts at: 3241 3241 Size: 7213 KpnI G_GTAC'C Cuts at: 4538 4538 Size: 7213 MfeI C'AATT_G Cuts at: 4812 4812 Size: 7213 MluI A'CGCG_T Cuts at: 7089 7089 Size: 7213 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3846 6327 6511 1305 Size: 2541 2481 184 2007 Fragments arranged by size: 2541 2481 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3218 10 Size: 54 1425 343 1386 4005 Fragments arranged by size: 4005 1425 1386 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2618 5171 5530 5689 518 Size: 648 1452 2553 359 159 2042 Fragments arranged by size: 2553 2042 1452 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3214 3222 3425 3705 4629 5306 6454 6699 Size: 1608 8 203 280 924 677 1148 245 Cuts at: 6699 1606 Size: 2120 Fragments arranged by size: 2120 1608 1148 924 677 280 245 203 8 NaeI GCC'GGC Cuts at: 3203 3203 Size: 7213 NarI GG'CG_CC Cuts at: 3242 3242 Size: 7213 NcoI C'CATG_G Cuts at: 513 1065 2967 3531 4345 513 Size: 552 1902 564 814 3381 Fragments arranged by size: 3381 1902 814 564 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7213 NgoMIV G'CCGG_C Cuts at: 3201 3201 Size: 7213 NheI G'CTAG_C Cuts at: 1052 3898 4289 1052 Size: 2846 391 3976 Fragments arranged by size: 3976 2846 391 NotI GC'GGCC_GC Cuts at: 4660 4660 Size: 7213 NsiI A_TGCA'T Cuts at: 2685 2685 Size: 7213 NspI r_CATG'y Cuts at: 1132 1229 2678 3761 4571 6116 1132 Size: 97 1449 1083 810 1545 2229 Fragments arranged by size: 2229 1545 1449 1083 810 97 PasI CC'CwG_GG Cuts at: 1348 4001 1348 Size: 2653 4560 PciI A'CATG_T Cuts at: 6112 6112 Size: 7213 PflMI CCAn_nnn'nTGG Cuts at: 1108 3098 4178 1108 Size: 1990 1080 4143 Fragments arranged by size: 4143 1990 1080 PfoI T'CCnGG_A Cuts at: 4022 4022 Size: 7213 PmeI GTTT'AAAC Cuts at: 4517 4517 Size: 7213 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2889 2921 5282 5314 6795 6827 2889 Size: 32 2361 32 1481 32 3275 Fragments arranged by size: 3275 2361 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2395 3584 2395 Size: 1189 6024 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7213 PsiI TTA'TAA Cuts at: 2559 4783 2559 Size: 2224 4989 PspOMI G'GGCC_C Cuts at: 2352 2643 4439 2352 Size: 291 1796 5126 Fragments arranged by size: 5126 1796 291 PstI C_TGCA'G Cuts at: 830 4360 4565 830 Size: 3530 205 3478 Fragments arranged by size: 3530 3478 205 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7213 PvuII CAG'CTG Cuts at: 3425 3425 Size: 7213 SacI G_AGCT'C Cuts at: 2190 2190 Size: 7213 SalI G'TCGA_C Cuts at: 1936 4555 1936 Size: 2619 4594 SbfI CC_TGCA'GG Cuts at: 4565 4565 Size: 7213 ScaI AGT'ACT Cuts at: 5461 7173 5461 Size: 1712 5501 SexAI A'CCwGG_T Cuts at: 3097 3097 Size: 7213 SfcI C'TryA_G Cuts at: 826 1045 3507 3916 4356 4365 4462 4561 5696 Size: 219 2462 409 440 9 97 99 1135 Cuts at: 5696 6377 6568 826 Size: 681 191 1471 Fragments arranged by size: 2462 1471 1135 681 440 409 219 191 99 97 9 SfoI GGC'GCC Cuts at: 3243 3243 Size: 7213 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7213 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7213 SmaI CCC'GGG Cuts at: 1872 4540 1872 Size: 2668 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2363 2716 2768 2787 3175 5317 Size: 197 926 420 353 52 19 388 2142 Cuts at: 5317 6218 6480 820 Size: 901 262 1553 Fragments arranged by size: 2142 1553 926 901 420 388 353 262 197 52 19 SnaBI TAC'GTA Cuts at: 493 4510 5004 493 Size: 4017 494 2702 Fragments arranged by size: 4017 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7213 SphI G_CATG'C Cuts at: 1132 3761 4571 1132 Size: 2629 810 3774 Fragments arranged by size: 3774 2629 810 SspI AAT'ATT Cuts at: 5 52 5137 5 Size: 47 5085 2081 Fragments arranged by size: 5085 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7213 StyI C'CwwG_G Cuts at: 513 1065 2120 2698 2967 3129 3531 4345 4652 Size: 552 1055 578 269 162 402 814 307 Cuts at: 4652 513 Size: 3074 Fragments arranged by size: 3074 1055 814 578 552 402 307 269 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5240 5257 5410 5595 6040 325 Size: 1255 3660 17 153 185 445 1498 Fragments arranged by size: 3660 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3841 4096 5459 Size: 274 80 33 51 1443 1864 255 1363 Cuts at: 5459 7171 96 Size: 1712 138 Fragments arranged by size: 1864 1712 1443 1363 274 255 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3248 3280 7173 7205 3248 Size: 32 3893 32 3256 Fragments arranged by size: 3893 3256 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7213 XbaI T'CTAG_A Cuts at: 4549 4549 Size: 7213 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3529 3529 Size: 7213 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7213 XmaI C'CCGG_G Cuts at: 1870 4538 1870 Size: 2668 4545 XmnI GAAnn'nnTTC Cuts at: 2298 2904 2988 5342 2298 Size: 606 84 2354 4169 Fragments arranged by size: 4169 2354 606 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3499 5021 276 Size: 53 83 186 936 1965 1522 2468 Fragments arranged by size: 2468 1965 1522 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BplI BsiWI BstZ17I Bsu36I DraIII FspAI NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI