(Circular) (Six-base) MAPSORT of: pFN21ASDA0007.seq Check: 8853 from: 1 to: 6760 pFN21ASDA0007 6760 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4570 278 Size: 53 83 186 936 3034 2468 Fragments arranged by size: 3034 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2622 4103 1937 Size: 685 1481 4594 Fragments arranged by size: 4594 1481 685 Acc65I G'GTAC_C Cuts at: 2201 3655 4081 2201 Size: 1454 426 4880 Fragments arranged by size: 4880 1454 426 AclI AA'CG_TT Cuts at: 1563 4887 5260 1563 Size: 3324 373 3063 Fragments arranged by size: 3324 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3240 3855 3894 4823 6207 6475 1632 Size: 1608 615 39 929 1384 268 1917 Fragments arranged by size: 1917 1608 1384 929 615 268 39 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6563 AflIII A'CryG_T Cuts at: 1203 5659 6636 1203 Size: 4456 977 1327 Fragments arranged by size: 4456 1327 977 AgeI A'CCGG_T Cuts at: 2751 5591 2751 Size: 2840 3920 AhdI GACnn_n'nnGTC Cuts at: 3055 5489 3055 Size: 2434 4326 AleI CACnn'nnGTG Cuts at: 1166 2196 1166 Size: 1030 5730 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6760 AlwNI CAG_nnn'CTG Cuts at: 2282 2439 4503 6075 2282 Size: 157 2064 1572 2967 Fragments arranged by size: 2967 2064 1572 157 ApaI G_GGCC'C Cuts at: 3169 3169 Size: 6760 ApaLI G'TGCA_C Cuts at: 4817 5973 4817 Size: 1156 5604 ApoI r'AATT_y Cuts at: 1493 3214 4069 4295 4447 6556 1493 Size: 1721 855 226 152 2109 1697 Fragments arranged by size: 2109 1721 1697 855 226 152 AseI AT'TA_AT Cuts at: 160 1032 2381 5314 160 Size: 872 1349 2933 1606 Fragments arranged by size: 2933 1606 1349 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6760 AvaI C'yCGr_G Cuts at: 1870 1943 4085 1870 Size: 73 2142 4545 Fragments arranged by size: 4545 2142 73 BamHI G'GATC_C Cuts at: 4090 4457 4090 Size: 367 6393 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2201 2224 3235 3655 4081 Size: 325 217 627 414 23 1011 420 426 Cuts at: 4081 5536 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1011 627 426 420 414 325 217 23 BanII G_rGCy'C Cuts at: 1391 3169 4079 1391 Size: 1778 910 4072 Fragments arranged by size: 4072 1778 910 BbeI G_GCGC'C Cuts at: 2228 2228 Size: 6760 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3393 3503 3779 3822 928 Size: 971 1494 110 276 43 3866 Fragments arranged by size: 3866 1494 971 276 110 43 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4951 4985 1903 Size: 34 3014 34 3678 Fragments arranged by size: 3678 3014 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4652 5862 4652 Size: 1210 5550 BclI T'GATC_A Cuts at: 1548 1548 Size: 6760 BfrBI ATG'CAT Cuts at: 3626 3626 Size: 6760 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2233 5371 136 Size: 107 122 71 631 605 561 3138 1525 Fragments arranged by size: 3138 1525 631 605 561 122 107 71 BglII A'GATC_T Cuts at: 6755 6755 Size: 6760 BlpI GC'TnA_GC Cuts at: 4177 4177 Size: 6760 Bme1580I G_kGCm'C Cuts at: 1790 3169 4821 5977 1790 Size: 1379 1652 1156 2573 Fragments arranged by size: 2573 1652 1379 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6760 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4491 5449 442 Size: 416 53 83 399 24 3074 958 1753 Fragments arranged by size: 3074 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6760 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2757 3103 3377 5420 1239 Size: 1518 346 274 2043 2579 Fragments arranged by size: 2579 2043 1518 346 274 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4885 5750 6048 4885 Size: 865 298 5597 Fragments arranged by size: 5597 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2603 5423 882 Size: 617 1104 2820 2219 Fragments arranged by size: 2820 2219 1104 617 BsaAI yAC'GTr Cuts at: 493 1204 2179 4057 4551 493 Size: 711 975 1878 494 2702 Fragments arranged by size: 2702 1878 975 711 494 BsaBI GATnn'nnATC Cuts at: 6754 6754 Size: 6760 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2225 4567 4949 5584 Size: 53 83 186 936 692 2342 382 635 Cuts at: 5584 275 Size: 1451 Fragments arranged by size: 2342 1451 936 692 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2751 5193 5591 5865 6012 6423 2751 Size: 2442 398 274 147 411 3088 Fragments arranged by size: 3088 2442 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6535 Fragments arranged by size: 6535 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3267 3854 3911 3914 1186 Size: 2081 587 57 3 4032 Fragments arranged by size: 4032 2081 587 57 3 BseYI C'CCAG_C Cuts at: 2066 3162 3660 3664 5963 6459 2066 Size: 1096 498 4 2299 496 2367 Fragments arranged by size: 2367 2299 1096 498 496 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2150 2191 2820 2950 1163 Size: 573 414 41 629 130 4973 Fragments arranged by size: 4973 629 573 414 130 41 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4210 4971 5999 1540 Size: 275 186 2209 761 1028 2301 Fragments arranged by size: 2301 2209 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4821 4906 5977 4821 Size: 85 1071 5604 Fragments arranged by size: 5604 1071 85 BsmI GAATG_Cn' Cuts at: 4271 4364 4271 Size: 93 6667 BsmBI CGTCTCn'nnnn_ Cuts at: 2141 2141 Size: 6760 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3169 4079 4821 4906 5977 1391 Size: 399 1379 910 742 85 1071 2174 Fragments arranged by size: 2174 1379 1071 910 742 399 85 BspEI T'CCGG_A Cuts at: 6423 6423 Size: 6760 BspHI T'CATG_A Cuts at: 4649 6379 4649 Size: 1730 5030 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2213 2761 4115 844 Size: 1369 548 1354 3489 Fragments arranged by size: 3489 1369 1354 548 BsrBI CCG'CTC Cuts at: 1394 1823 3275 4647 1394 Size: 429 1452 1372 3507 Fragments arranged by size: 3507 1452 1372 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3360 5255 5429 65 Size: 1183 2112 1895 174 1396 Fragments arranged by size: 2112 1895 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2160 2751 5404 5591 1700 Size: 110 350 591 2653 187 2869 Fragments arranged by size: 2869 2653 591 350 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6760 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6760 BssSI C'ACGA_G Cuts at: 4820 5832 4820 Size: 1012 5748 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6760 BstBI TT'CG_AA Cuts at: 2007 5564 2007 Size: 3557 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6760 BstYI r'GATC_y Cuts at: 1972 4027 4090 4457 4842 4859 6311 6397 6409 Size: 2055 63 367 385 17 1452 86 12 Cuts at: 6409 6755 1972 Size: 346 1977 Fragments arranged by size: 2055 1977 1452 385 367 346 86 63 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5251 Fragments arranged by size: 5251 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6021 Fragments arranged by size: 6021 683 56 BtsI GCAGTG_nn' Cuts at: 2536 2808 4276 5070 5090 2536 Size: 272 1468 794 20 4206 Fragments arranged by size: 4206 1468 794 272 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6760 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6725 DraI TTT'AAA Cuts at: 3114 4064 3114 Size: 950 5810 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2405 5767 809 Size: 507 1089 3362 1802 Fragments arranged by size: 3362 1802 1089 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2151 4207 5096 Size: 54 1425 50 275 18 321 2056 889 Cuts at: 5096 6725 8 Size: 1629 43 Fragments arranged by size: 2056 1629 1425 889 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4207 1537 Size: 275 2395 4090 Fragments arranged by size: 4090 2395 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2862 3819 3831 3927 4690 1353 Size: 1509 957 12 96 763 3423 Fragments arranged by size: 3423 1509 957 763 96 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5347 5721 5867 387 Size: 1064 138 3758 374 146 1280 Fragments arranged by size: 3758 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2757 3103 3240 3377 3855 3894 4823 Size: 393 1125 346 137 137 478 39 929 Cuts at: 4823 5420 6207 6475 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1125 929 787 597 478 393 346 268 137 137 39 EcoNI CCTnn'n_nnAGG Cuts at: 2991 2991 Size: 6760 EcoO109I rG'GnC_Cy Cuts at: 2071 3166 2071 Size: 1095 5665 EcoRI G'AATT_C Cuts at: 3214 4069 4447 3214 Size: 855 378 5527 Fragments arranged by size: 5527 855 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2930 2930 Size: 6760 FspI TGC'GCA Cuts at: 5266 5266 Size: 6760 HaeII r_GCGC'y Cuts at: 1678 2228 5907 1678 Size: 550 3679 2531 Fragments arranged by size: 3679 2531 550 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3041 3073 3235 3267 5401 Size: 32 135 32 1469 32 162 32 2134 Cuts at: 5401 5433 5475 5507 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2134 1469 162 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2410 3006 3615 4104 4350 669 Size: 988 281 472 596 609 489 246 3079 Fragments arranged by size: 3079 988 609 596 489 472 281 246 HindIII A'AGCT_T Cuts at: 748 3734 748 Size: 2986 3774 HpaI GTT'AAC Cuts at: 3615 4350 3615 Size: 735 6025 KasI G'GCGC_C Cuts at: 2224 2224 Size: 6760 KpnI G_GTAC'C Cuts at: 2205 3659 4085 2205 Size: 1454 426 4880 Fragments arranged by size: 4880 1454 426 MfeI C'AATT_G Cuts at: 4359 4359 Size: 6760 MluI A'CGCG_T Cuts at: 6636 6636 Size: 6760 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5874 6058 1305 Size: 4569 184 2007 Fragments arranged by size: 4569 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4938 Fragments arranged by size: 4938 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2196 3726 4718 5077 5236 518 Size: 648 1030 1530 992 359 159 2042 Fragments arranged by size: 2042 1530 1030 992 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2579 3664 4176 4853 6001 6246 1606 Size: 973 1085 512 677 1148 245 2120 Fragments arranged by size: 2120 1148 1085 973 677 512 245 NarI GG'CG_CC Cuts at: 2225 2225 Size: 6760 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5251 Fragments arranged by size: 5251 957 552 NdeI CA'TA_TG Cuts at: 387 3132 387 Size: 2745 4015 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6760 NotI GC'GGCC_GC Cuts at: 4207 4207 Size: 6760 NsiI A_TGCA'T Cuts at: 3628 3628 Size: 6760 NspI r_CATG'y Cuts at: 1132 1229 2496 2645 4118 5663 1132 Size: 97 1267 149 1473 1545 2229 Fragments arranged by size: 2229 1545 1473 1267 149 97 PasI CC'CwG_GG Cuts at: 1348 3424 1348 Size: 2076 4684 PciI A'CATG_T Cuts at: 5659 5659 Size: 6760 PflMI CCAn_nnn'nTGG Cuts at: 1108 3685 1108 Size: 2577 4183 PmeI GTTT'AAAC Cuts at: 4064 4064 Size: 6760 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4829 4861 6342 6374 4829 Size: 32 1481 32 5215 Fragments arranged by size: 5215 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2071 2071 Size: 6760 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6760 PsiI TTA'TAA Cuts at: 3491 4330 3491 Size: 839 5921 PspOMI G'GGCC_C Cuts at: 3165 3165 Size: 6760 PstI C_TGCA'G Cuts at: 830 2241 4112 830 Size: 1411 1871 3478 Fragments arranged by size: 3478 1871 1411 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6760 PvuII CAG'CTG Cuts at: 2579 3664 2579 Size: 1085 5675 SalI G'TCGA_C Cuts at: 1936 4102 1936 Size: 2166 4594 SanDI GG'GwC_CC Cuts at: 2071 2071 Size: 6760 SapI GCTCTTCn'nnn_ Cuts at: 2862 2862 Size: 6760 SbfI CC_TGCA'GG Cuts at: 4112 4112 Size: 6760 ScaI AGT'ACT Cuts at: 3465 5008 6720 3465 Size: 1543 1712 3505 Fragments arranged by size: 3505 1712 1543 SexAI A'CCwGG_T Cuts at: 3651 3651 Size: 6760 SfcI C'TryA_G Cuts at: 826 1045 2237 2283 2623 4108 5243 5924 6115 Size: 219 1192 46 340 1485 1135 681 191 Cuts at: 6115 826 Size: 1471 Fragments arranged by size: 1485 1471 1192 1135 681 340 219 191 46 SfoI GGC'GCC Cuts at: 2226 2226 Size: 6760 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6760 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6760 SmaI CCC'GGG Cuts at: 1872 4087 1872 Size: 2215 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4864 5765 6027 820 Size: 197 926 2921 901 262 1553 Fragments arranged by size: 2921 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4057 4551 493 Size: 3564 494 2702 Fragments arranged by size: 3564 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6760 SphI G_CATG'C Cuts at: 1132 4118 1132 Size: 2986 3774 SspI AAT'ATT Cuts at: 5 52 4684 5 Size: 47 4632 2081 Fragments arranged by size: 4632 2081 47 StuI AGG'CCT Cuts at: 1517 2102 1517 Size: 585 6175 StyI C'CwwG_G Cuts at: 513 1065 2022 2244 4199 513 Size: 552 957 222 1955 3074 Fragments arranged by size: 3074 1955 957 552 222 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4787 4804 4957 5142 5587 325 Size: 1255 3207 17 153 185 445 1498 Fragments arranged by size: 3207 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2743 3463 5006 Size: 274 80 33 51 1443 766 720 1543 Cuts at: 5006 6718 96 Size: 1712 138 Fragments arranged by size: 1712 1543 1443 766 720 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6720 6752 6720 Size: 32 6728 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6760 XbaI T'CTAG_A Cuts at: 4096 4096 Size: 6760 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2705 2705 Size: 6760 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6760 XmaI C'CCGG_G Cuts at: 1870 4085 1870 Size: 2215 4545 XmnI GAAnn'nnTTC Cuts at: 4889 4889 Size: 6760 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4568 276 Size: 53 83 186 936 3034 2468 Fragments arranged by size: 3034 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SfiI SrfI SwaI