Restriction map for
pFN21AB8019
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AlwNI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BciVI BclI
BfaI BfrI BglI BlnI BsgI
BsiWI BsmI BspDI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI DraI DrdI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FspI HpaI KpnI
KspI MaeI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 647 TCCGGA T^CCGGA
AcsI 136 AAATTC [GA]^AATT[CT]
Alw44I 124 GTGCAC G^TGCAC
ApaLI 124 GTGCAC G^TGCAC
ApoI 136 AAATTC [GA]^AATT[CT]
BbeI 1143 GGCGCC GGCGC^C
BbrPI 1294 CACGTG CAC^GTG
BbsI 1411 GTCTTC GAAGAC(2/6)
BglII 185 AGATCT A^GATCT
BpuAI 1411 GTCTTC GAAGAC(2/6)
BsaAI 1294 CACGTG [CT]AC^GT[GA]
BsaBI 1239 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 647 TCCGGA [AT]^CCGG[AT]
BseAI 647 TCCGGA T^CCGGA
Bsp1407I 1286 TGTACA T^GTACA
BspEI 647 TCCGGA T^CCGGA
BspHI 848 TCATGA T^CATGA
BspLU11I 896 ACATGT A^CATGT
BsrDI 938 GCAATG GCAATG(2/0)
BsrGI 1286 TGTACA T^GTACA
BssSI 597 CACGAG CACGAG(-5/-1)
CpoI 692 CGGACCG CG^G[AT]CCG
DraIII 1748 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
FseI 1785 GGCCGGCC GGCCGG^CC
HincII 1375 GTCAAC GT[CT]^[GA]AC
HindII 1375 GTCAAC GT[CT]^[GA]AC
HindIII 205 AAGCTT A^AGCTT
KasI 1143 GGCGCC G^GCGCC
MamI 1239 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 647 TCCGGA T^CCGGA
MseI 146 TTAA T^TAA
NarI 1143 GGCGCC GG^CGCC
PflMI 1007 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1294 CACGTG CAC^GTG
PmlI 1294 CACGTG CAC^GTG
RcaI 848 TCATGA T^CATGA
RsrII 692 CGGACCG CG^G[AT]CCG
SexAI 50 ACCTGGT A^CC[AT]GGT
SfoI 1143 GGCGCC GGC^GCC
SspBI 1286 TGTACA T^GTACA
TfiI 1732 GAATC G^A[AT]TC
Tru9I 146 TTAA T^TAA
Van91I 1007 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 896 ACATGT A^C[GA][CT]GT
1293 ACACGT
AhdI 526 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1904 GACCCCCTGTC
ApaI 985 GGGCCC GGGCC^C
1676 GGGCCC
AspEI 526 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1904 GACCCCCTGTC
BalI 371 TGGCCA TGG^CCA
1004 TGGCCA
BbvCI 543 CCTCAGC CCTCAGC(-5/-2)
250 GCTGAGG
BpmI 1461 CTCCAG CTGGAG(16/14)
1210 CTGGAG
BsmBI 40 GAGACG CGTCTC(1/5)
821 GAGACG
BspMI 302 ACCTGC ACCTGC(4/8)
906 GCAGGT
BsrBI 319 GAGCGG CCGCTC(-3/-3)
1312 GAGCGG
Eam1105I 526 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1904 GACCCCCTGTC
EarI 1138 GAAGAG CTCTTC(1/4)
1158 GAAGAG
EcoNI 246 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
681 CCTTCGGCAGG
Ksp632I 1138 GAAGAG CTCTTC(1/4)
1158 GAAGAG
MluNI 371 TGGCCA TGG^CCA
1004 TGGCCA
MscI 371 TGGCCA TGG^CCA
1004 TGGCCA
NaeI 662 GCCGGC GCC^GGC
1786 GCCGGC
NcoI 152 CCATGG C^CATGG
416 CCATGG
NgoMIV 662 GCCGGC G^CCGGC
1786 GCCGGC
NspI 896 ACATGT [GA]CATG^[CT]
1226 GCATGT
PstI 904 CTGCAG CTGCA^G
1425 CTGCAG
PvuII 1192 CAGCTG CAG^CTG
1767 CAGCTG
SfcI 904 CTGCAG C^T[GA][CT]AG
1425 CTGCAG
SgrAI 427 CACCGGCG C[GA]^CCGG[CT]G
661 CGCCGGCG
StuI 1823 AGGCCT AGG^CCT
1871 AGGCCT
TaqI 884 TCGA T^CGA
1073 TCGA
Tsp509I 137 AATT ^AATT
1940 AATT
TthHB8I 884 TCGA T^CGA
1073 TCGA
XcmI 563 CCAGCAGCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1791 CCAGCTCAGCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BanI
BanII BbvI BcgI BcnI BlpI
BmyI Bpu1102I BsaHI BsaI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SmaI
SmlI StyI TseI Tsp45I TspRI
XhoII XmaCI XmaI