Restriction map for pFN21AB8019
 (Linear) (Six-base) MAPSORT of: KIBB8019.seq  Check: 3510  from: 1  to: 2010



KIBB8019.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     897    2010 
   Size:        897    1113
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     693    1369    2010 
   Size:        693     676     641

  Fragments arranged by size: 

                 693     676     641
 
AflIII A'CryG_T

Cuts at:      0     896    1293    2010 
   Size:        896     397     717

  Fragments arranged by size: 

                 896     717     397
 
AhdI GACnn_n'nnGTC

Cuts at:      0     531    1909    2010 
   Size:        531    1378     101

  Fragments arranged by size: 

                1378     531     101
 
AleI CACnn'nnGTG

Cuts at:      0    1294    2010 
   Size:       1294     716
 
ApaI G_GGCC'C

Cuts at:      0     989    1680    2010 
   Size:        989     691     330

  Fragments arranged by size: 

                 989     691     330
 
ApaLI G'TGCA_C

Cuts at:      0     124    2010 
   Size:        124    1886
 
ApoI r'AATT_y

Cuts at:      0     136    2010 
   Size:        136    1874
 
AvaI C'yCGr_G

Cuts at:      0      73    1203    1551    1590    1680    1839    1897    2010 
   Size:         73    1130     348      39      90     159      58     113

  Fragments arranged by size: 

                1130     348     159     113      90      73      58      39
            
 
BanI G'GyrC_C

Cuts at:      0     482    1143    1746    2010 
   Size:        482     661     603     264

  Fragments arranged by size: 

                 661     603     482     264
 
BanII G_rGCy'C

Cuts at:      0     890     989    1119    1680    1847    2010 
   Size:        890      99     130     561     167     163

  Fragments arranged by size: 

                 890     561     167     163     130      99
 
BbeI G_GCGC'C

Cuts at:      0    1147    2010 
   Size:       1147     863
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1404    2010 
   Size:       1404     606
 
BbvCI CC'TCA_GC

Cuts at:      0     251     544    2010 
   Size:        251     293    1466

  Fragments arranged by size: 

                1466     293     251
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     664     698     777     811    1416    1450    2010 
   Size:        664      34      79      34     605      34     560

  Fragments arranged by size: 

                 664     605     560      79      34      34      34
 
BglII A'GATC_T

Cuts at:      0     185    2010 
   Size:        185    1825
 
BlpI GC'TnA_GC

Cuts at:      0     109     607    1795    2010 
   Size:        109     498    1188     215

  Fragments arranged by size: 

                1188     498     215     109
 
Bme1580I G_kGCm'C

Cuts at:      0     128     286     444     485     989    1680    2010 
   Size:        128     158     158      41     504     691     330

  Fragments arranged by size: 

                 691     504     330     158     158     128      41
 
BmgBI CAC'GTC

Cuts at:      0     918    1272    2010 
   Size:        918     354     738

  Fragments arranged by size: 

                 918     738     354
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1231    1446    2010 
   Size:       1231     215     564

  Fragments arranged by size: 

                1231     564     215
 
Bpu10I CC'TnA_GC

Cuts at:      0     251     544    2010 
   Size:        251     293    1466

  Fragments arranged by size: 

                1466     293     251
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     577    1219    2010 
   Size:        337     240     642     791

  Fragments arranged by size: 

                 791     642     337     240
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       1     237     488    2010 
   Size:          1     236     251    1522

  Fragments arranged by size: 

                1522     251     236       1
 
BsaAI yAC'GTr

Cuts at:      0    1296    2010 
   Size:       1296     714
 
BsaBI GATnn'nnATC

Cuts at:      0    1243    2010 
   Size:       1243     767
 
BsaHI Gr'CG_yC

Cuts at:      0      43     447    1144    2010 
   Size:         43     404     697     866

  Fragments arranged by size: 

                 866     697     404      43
 
BsaWI w'CCGG_w

Cuts at:      0     647    2010 
   Size:        647    1363
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      31      61     685     715     826     856    1444    1474 
   Size:         31      30     624      30     111      30     588      30

Cuts at:   1474    2010 
   Size:        536

  Fragments arranged by size: 

                 624     588     536     111      31      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49      52     196     475     693    2010 
   Size:         49       3     144     279     218    1317

  Fragments arranged by size: 

                1317     279     218     144      49       3
 
BseYI C'CCAG_C

Cuts at:      0    1013    1714    2010 
   Size:       1013     701     296

  Fragments arranged by size: 

                1013     701     296
 
BsiEI CG_ry'CG

Cuts at:      0     225     816     879    1224    1787    2010 
   Size:        225     591      63     345     563     223

  Fragments arranged by size: 

                 591     563     345     225     223      63
 
BsiHKAI G_wGCw'C

Cuts at:      0     110     128     261     631    2010 
   Size:        110      18     133     370    1379

  Fragments arranged by size: 

                1379     370     133     110      18
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      34     815    2010 
   Size:         34     781    1195

  Fragments arranged by size: 

                1195     781      34
 
Bsp1286I G_dGCh'C

Cuts at:      0     110     128     261     286     444     485     631     890 
   Size:        110      18     133      25     158      41     146     259

Cuts at:    890     989    1119    1680    1847    2010 
   Size:         99     130     561     167     163

  Fragments arranged by size: 

                 561     259     167     163     158     146     133     130
                 110      99      41      25      18
 
BspEI T'CCGG_A

Cuts at:      0     647    2010 
   Size:        647    1363
 
BspHI T'CATG_A

Cuts at:      0     848    2010 
   Size:        848    1162
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     311     897    2010 
   Size:        311     586    1113

  Fragments arranged by size: 

                1113     586     311
 
BsrBI CCG'CTC

Cuts at:      0     321    1314    2010 
   Size:        321     993     696

  Fragments arranged by size: 

                 993     696     321
 
BsrDI GCAATG_nn'

Cuts at:      0     945    2010 
   Size:        945    1065
 
BsrFI r'CCGG_y

Cuts at:      0     116     428     662    1786    2010 
   Size:        116     312     234    1124     224

  Fragments arranged by size: 

                1124     312     234     224     116
 
BsrGI T'GTAC_A

Cuts at:      0    1286    2010 
   Size:       1286     724
 
BssSI C'ACGA_G

Cuts at:      0     597    2010 
   Size:        597    1413
 
BstYI r'GATC_y

Cuts at:      0     185    1214    1419    2010 
   Size:        185    1029     205     591

  Fragments arranged by size: 

                1029     591     205     185
 
BtgI C'CryG_G

Cuts at:      0      65     152     416     617     816    1048    1800    2010 
   Size:         65      87     264     201     199     232     752     210

  Fragments arranged by size: 

                 752     264     232     210     201     199      87      65
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1230    2010 
   Size:       1230     780
 
BtsI GCAGTG_nn'

Cuts at:      0    1405    1656    1728    1734    2010 
   Size:       1405     251      72       6     276

  Fragments arranged by size: 

                1405     276     251      72       6
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     798     833    1632    1667    2010 
   Size:        798      35     799      35     343

  Fragments arranged by size: 

                 799     798     343      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1753    2010 
   Size:       1753     257
 
EaeI y'GGCC_r

Cuts at:      0      89     222     371     866     876    1004    1784    1788 
   Size:         89     133     149     495      10     128     780       4

Cuts at:   1788    2010 
   Size:        222

  Fragments arranged by size: 

                 780     495     222     149     133     128      89      10
                   4
 
EagI C'GGCC_G

Cuts at:      0     222     876    1784    2010 
   Size:        222     654     908     226

  Fragments arranged by size: 

                 908     654     226     222
 
EarI CTCTTCn'nnn_

Cuts at:      0    1133    1153    2010 
   Size:       1133      20     857

  Fragments arranged by size: 

                1133     857      20
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     681     706    1282    2010 
   Size:         19     662      25     576     728

  Fragments arranged by size: 

                 728     662     576      25      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     693    1231    1369    1446    2010 
   Size:        693     538     138      77     564

  Fragments arranged by size: 

                 693     564     538     138      77
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     250     685    2010 
   Size:        250     435    1325

  Fragments arranged by size: 

                1325     435     250
 
EcoO109I rG'GnC_Cy

Cuts at:      0     275     985    1017    1555    1587    1677    1903    2010 
   Size:        275     710      32     538      32      90     226     107

  Fragments arranged by size: 

                 710     538     275     226     107      90      32      32
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1374    1404    2010 
   Size:       1374      30     606

  Fragments arranged by size: 

                1374     606      30
 
FseI GG_CCGG'CC

Cuts at:      0    1790    2010 
   Size:       1790     220
 
HaeII r_GCGC'y

Cuts at:      0     681    1147    1175    2010 
   Size:        681     466      28     835

  Fragments arranged by size: 

                 835     681     466      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     259     291     517     544     549     576     685     717 
   Size:        259      32     226      27       5      27     109      32

Cuts at:    717    1199    1231    1461    1493    1895    1927    2010 
   Size:        482      32     230      32     402      32      83

  Fragments arranged by size: 

                 482     402     259     230     226     109      83      32
                  32      32      32      32      27      27       5
 
HincII GTy'rAC

Cuts at:      0    1377    2010 
   Size:       1377     633
 
HindIII A'AGCT_T

Cuts at:      0     205    2010 
   Size:        205    1805
 
KasI G'GCGC_C

Cuts at:      0    1143    2010 
   Size:       1143     867
 
MscI TGG'CCA

Cuts at:      0     373    1006    2010 
   Size:        373     633    1004

  Fragments arranged by size: 

                1004     633     373
 
MslI CAynn'nnrTG

Cuts at:      0     266     365    1294    2010 
   Size:        266      99     929     716

  Fragments arranged by size: 

                 929     716     266      99
 
MspA1I CmG'CkG

Cuts at:      0     871    1149    1194    1497    1610    1769    2010 
   Size:        871     278      45     303     113     159     241

  Fragments arranged by size: 

                 871     303     278     241     159     113      45
 
NaeI GCC'GGC

Cuts at:      0     664    1788    2010 
   Size:        664    1124     222

  Fragments arranged by size: 

                1124     664     222
 
NarI GG'CG_CC

Cuts at:      0    1144    2010 
   Size:       1144     866
 
NcoI C'CATG_G

Cuts at:      0     152     416    2010 
   Size:        152     264    1594

  Fragments arranged by size: 

                1594     264     152
 
NgoMIV G'CCGG_C

Cuts at:      0     662    1786    2010 
   Size:        662    1124     224

  Fragments arranged by size: 

                1124     662     224
 
NspI r_CATG'y

Cuts at:      0     900    1230    2010 
   Size:        900     330     780

  Fragments arranged by size: 

                 900     780     330
 
PasI CC'CwG_GG

Cuts at:      0     279    2010 
   Size:        279    1731
 
PciI A'CATG_T

Cuts at:      0     896    2010 
   Size:        896    1114
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1013    2010 
   Size:       1013     997
 
PfoI T'CCnGG_A

Cuts at:      0    1913    2010 
   Size:       1913      97
 
PmlI CAC'GTG

Cuts at:      0    1296    2010 
   Size:       1296     714
 
PpuMI rG'GwC_Cy

Cuts at:      0     275    1017    1555    1587    1903    2010 
   Size:        275     742     538      32     316     107

  Fragments arranged by size: 

                 742     538     316     275     107      32
 
PspOMI G'GGCC_C

Cuts at:      0     985    1676    2010 
   Size:        985     691     334

  Fragments arranged by size: 

                 985     691     334
 
PstI C_TGCA'G

Cuts at:      0     908    1429    2010 
   Size:        908     521     581

  Fragments arranged by size: 

                 908     581     521
 
PvuII CAG'CTG

Cuts at:      0    1194    1769    2010 
   Size:       1194     575     241

  Fragments arranged by size: 

                1194     575     241
 
RsrII CG'GwC_CG

Cuts at:      0     693    2010 
   Size:        693    1317
 
SanDI GG'GwC_CC

Cuts at:      0     275    1017    1555    1903    2010 
   Size:        275     742     538     348     107

  Fragments arranged by size: 

                 742     538     348     275     107
 
SexAI A'CCwGG_T

Cuts at:      0      50    2010 
   Size:         50    1960
 
SfcI C'TryA_G

Cuts at:      0     904    1425    2010 
   Size:        904     521     585

  Fragments arranged by size: 

                 904     585     521
 
SfoI GGC'GCC

Cuts at:      0    1145    2010 
   Size:       1145     865
 
SgrAI Cr'CCGG_yG

Cuts at:      0     428     662    2010 
   Size:        428     234    1348

  Fragments arranged by size: 

                1348     428     234
 
SmaI CCC'GGG

Cuts at:      0    1553    1592    1682    1841    1899    2010 
   Size:       1553      39      90     159      58     111

  Fragments arranged by size: 

                1553     159     111      90      58      39
 
SmlI C'TyrA_G

Cuts at:      0     316     556    1234    2010 
   Size:        316     240     678     776

  Fragments arranged by size: 

                 776     678     316     240
 
StuI AGG'CCT

Cuts at:      0    1825    1873    2010 
   Size:       1825      48     137

  Fragments arranged by size: 

                1825     137      48
 
StyI C'CwwG_G

Cuts at:      0     152     416     487    1868    2010 
   Size:        152     264      71    1381     142

  Fragments arranged by size: 

                1381     264     152     142      71
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     500     512    2010 
   Size:        500      12    1498

  Fragments arranged by size: 

                1498     500      12
 
TatI w'GTAC_w

Cuts at:      0    1286    2010 
   Size:       1286     724
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1553    1585    2010 
   Size:       1553      32     425

  Fragments arranged by size: 

                1553     425      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     570    1798    2010 
   Size:        570    1228     212

  Fragments arranged by size: 

                1228     570     212
 
XmaI C'CCGG_G

Cuts at:      0    1551    1590    1680    1839    1897    2010 
   Size:       1551      39      90     159      58     113

  Fragments arranged by size: 

                1551     159     113      90      58      39
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI     AleI     ApaI    ApaLI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BglII
     BlpI Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI    BstYI     BtgI    BtgZI     BtsI    CspCI   DraIII
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI
     FseI    HaeII    Hin4I   HincII  HindIII     KasI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI
    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII    RsrII
    SanDI    SexAI     SfcI     SfoI    SgrAI     SmaI     SmlI     StuI
     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AlfI
     AloI    AlwNI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BciVI     BclI    BfrBI     BglI     BmrI     BmtI     BplI     BsgI
    BsiWI     BsmI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI     DraI     DrdI  EcoICRI    EcoRI    EcoRV     FspI
    FspAI     HpaI     KpnI     MfeI     MluI     MmeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PmeI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI     SacI    SacII     SalI     SapI     SbfI
     ScaI     SfiI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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