(Circular) (Six-base) MAPSORT of: pFN21AB8017.seq Check: 8240 from: 1 to: 6646 pFN21AB8017 6646 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2487 3855 2487 Size: 1368 5278 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4456 278 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3989 1937 Size: 2052 4594 Acc65I G'GTAC_C Cuts at: 3967 3967 Size: 6646 AclI AA'CG_TT Cuts at: 1563 4773 5146 1563 Size: 3210 373 3063 Fragments arranged by size: 3210 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2137 2330 4709 6093 6361 1632 Size: 505 193 2379 1384 268 1917 Fragments arranged by size: 2379 1917 1384 505 268 193 AfeI AGC'GCT Cuts at: 3521 3521 Size: 6646 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6449 AflIII A'CryG_T Cuts at: 1203 2342 5545 6522 1203 Size: 1139 3203 977 1327 Fragments arranged by size: 3203 1327 1139 977 AgeI A'CCGG_T Cuts at: 5477 5477 Size: 6646 AhdI GACnn_n'nnGTC Cuts at: 2540 5375 2540 Size: 2835 3811 AleI CACnn'nnGTG Cuts at: 1166 2381 2498 3530 1166 Size: 1215 117 1032 4282 Fragments arranged by size: 4282 1215 1032 117 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6646 AlwNI CAG_nnn'CTG Cuts at: 2125 2455 3653 4389 5961 2125 Size: 330 1198 736 1572 2810 Fragments arranged by size: 2810 1572 1198 736 330 ApaI G_GGCC'C Cuts at: 2239 3860 2239 Size: 1621 5025 ApaLI G'TGCA_C Cuts at: 4703 5859 4703 Size: 1156 5490 ApoI r'AATT_y Cuts at: 1493 2647 3955 4181 4333 6442 1493 Size: 1154 1308 226 152 2109 1697 Fragments arranged by size: 2109 1697 1308 1154 226 152 AseI AT'TA_AT Cuts at: 160 1032 5200 160 Size: 872 4168 1606 Fragments arranged by size: 4168 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 AvaI C'yCGr_G Cuts at: 1870 1943 2321 2765 3733 3776 3971 1870 Size: 73 378 444 968 43 195 4545 Fragments arranged by size: 4545 968 444 378 195 73 43 BamHI G'GATC_C Cuts at: 2617 3976 4343 2617 Size: 1359 367 4920 Fragments arranged by size: 4920 1359 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2579 3553 3967 5422 618 Size: 325 217 627 792 974 414 1455 1842 Fragments arranged by size: 1842 1455 974 792 627 414 325 217 BanII G_rGCy'C Cuts at: 1391 2239 2636 2828 2933 3860 3965 1391 Size: 848 397 192 105 927 105 4072 Fragments arranged by size: 4072 927 848 397 192 105 105 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3476 928 Size: 971 1577 4098 Fragments arranged by size: 4098 1577 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4837 4871 1903 Size: 34 2900 34 3678 Fragments arranged by size: 3678 2900 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4538 5748 4538 Size: 1210 5436 BclI T'GATC_A Cuts at: 1548 3564 1548 Size: 2016 4630 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2571 5257 136 Size: 107 122 71 631 605 899 2686 1525 Fragments arranged by size: 2686 1525 899 631 605 122 107 71 BglII A'GATC_T Cuts at: 6641 6641 Size: 6646 BlpI GC'TnA_GC Cuts at: 4063 4063 Size: 6646 Bme1580I G_kGCm'C Cuts at: 1790 2239 2412 2709 3860 4707 5863 1790 Size: 449 173 297 1151 847 1156 2573 Fragments arranged by size: 2573 1156 1151 847 449 297 173 BmgBI CAC'GTC Cuts at: 1318 2659 1318 Size: 1341 5305 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2242 2798 2936 Size: 416 53 83 399 24 825 556 138 Cuts at: 2936 3863 4377 5335 442 Size: 927 514 958 1753 Fragments arranged by size: 1753 958 927 825 556 514 416 399 138 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6646 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2053 2102 2738 3836 3887 5306 1239 Size: 814 49 636 1098 51 1419 2579 Fragments arranged by size: 2579 1419 1098 814 636 51 49 Bpu10I CC'TnA_GC Cuts at: 2822 2822 Size: 6646 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2905 3134 3179 3482 4771 5636 5934 2905 Size: 229 45 303 1289 865 298 3617 Fragments arranged by size: 3617 1289 865 303 298 229 45 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2658 2804 2913 3125 3375 3812 3887 Size: 617 1159 146 109 212 250 437 75 Cuts at: 3887 5309 882 Size: 1422 2219 Fragments arranged by size: 2219 1422 1159 617 437 250 212 146 109 75 BsaAI yAC'GTr Cuts at: 493 1204 2343 4437 493 Size: 711 1139 2094 2702 Fragments arranged by size: 2702 2094 1139 711 BsaBI GATnn'nnATC Cuts at: 6640 6640 Size: 6646 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4453 4835 5470 275 Size: 53 83 186 936 2920 382 635 1451 Fragments arranged by size: 2920 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2667 3117 5079 5477 5751 5898 6309 2667 Size: 450 1962 398 274 147 411 3004 Fragments arranged by size: 3004 1962 450 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2297 2327 3344 3374 3404 Size: 30 165 30 894 30 1017 30 30 Cuts at: 3404 1178 Size: 4420 Fragments arranged by size: 4420 1017 894 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2902 3143 1186 Size: 1716 241 4689 Fragments arranged by size: 4689 1716 241 BseYI C'CCAG_C Cuts at: 2300 2509 2800 2843 2943 3037 5849 6345 2300 Size: 209 291 43 100 94 2812 496 2601 Fragments arranged by size: 2812 2601 496 291 209 100 94 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6073 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3011 3574 4096 4857 5885 1540 Size: 275 186 1010 563 522 761 1028 2301 Fragments arranged by size: 2301 1028 1010 761 563 522 275 186 BsiHKAI G_wGCw'C Cuts at: 2267 2391 2589 2828 3195 3213 4707 4792 5863 Size: 124 198 239 367 18 1494 85 1071 Cuts at: 5863 2267 Size: 3050 Fragments arranged by size: 3050 1494 1071 367 239 198 124 85 18 BsmI GAATG_Cn' Cuts at: 4157 4250 4157 Size: 93 6553 BsmBI CGTCTCn'nnnn_ Cuts at: 3458 3458 Size: 6646 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2239 2267 2391 2412 2589 2636 2709 Size: 399 449 28 124 21 177 47 73 Cuts at: 2709 2828 2933 3195 3213 3860 3965 4707 4792 Size: 119 105 262 18 647 105 742 85 Cuts at: 4792 5863 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 647 449 399 262 177 124 119 105 105 85 73 47 28 21 18 BspEI T'CCGG_A Cuts at: 6309 6309 Size: 6646 BspHI T'CATG_A Cuts at: 4535 6265 4535 Size: 1730 4916 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2487 2733 3669 3855 4001 844 Size: 1643 246 936 186 146 3489 Fragments arranged by size: 3489 1643 936 246 186 146 BsrBI CCG'CTC Cuts at: 1394 1823 3915 4533 1394 Size: 429 2092 618 3507 Fragments arranged by size: 3507 2092 618 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5141 5315 65 Size: 1183 3893 174 1396 Fragments arranged by size: 3893 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2988 3839 3900 5290 5477 1700 Size: 110 1178 851 61 1390 187 2869 Fragments arranged by size: 2869 1390 1178 851 187 110 61 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6646 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6646 BssSI C'ACGA_G Cuts at: 4706 5718 4706 Size: 1012 5634 BstAPI GCAn_nnn'nTGC Cuts at: 794 2455 3048 794 Size: 1661 593 4392 Fragments arranged by size: 4392 1661 593 BstBI TT'CG_AA Cuts at: 5450 5450 Size: 6646 BstEII G'GTnAC_C Cuts at: 1184 2431 1184 Size: 1247 5399 BstXI CCAn_nnnn'nTGG Cuts at: 2287 2287 Size: 6646 BstYI r'GATC_y Cuts at: 1972 2097 2451 2617 2670 3976 4343 4728 4745 Size: 125 354 166 53 1306 367 385 17 Cuts at: 4745 6197 6283 6295 6641 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1306 385 367 354 346 166 125 86 53 17 12 Bsu36I CC'TnA_GG Cuts at: 3480 3933 3480 Size: 453 6193 BtgI C'CryG_G Cuts at: 513 1065 2257 2794 3025 3302 3528 3672 513 Size: 552 1192 537 231 277 226 144 3487 Fragments arranged by size: 3487 1192 552 537 277 231 226 144 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3184 3319 487 Size: 683 56 1958 135 3814 Fragments arranged by size: 3814 1958 683 135 56 BtsI GCAGTG_nn' Cuts at: 2176 2410 4162 4956 4976 2176 Size: 234 1752 794 20 3846 Fragments arranged by size: 3846 1752 794 234 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6646 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6611 DraI TTT'AAA Cuts at: 3950 3950 Size: 6646 DraIII CAC_nnn'GTG Cuts at: 3080 3080 Size: 6646 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5653 809 Size: 507 4337 1802 Fragments arranged by size: 4337 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2089 2107 2990 Size: 54 1425 50 275 18 259 18 883 Cuts at: 2990 3008 3055 3571 3837 3841 4093 4982 6611 Size: 18 47 516 266 4 252 889 1629 Cuts at: 6611 8 Size: 43 Fragments arranged by size: 1629 1425 889 883 516 275 266 259 252 54 50 47 43 18 18 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3008 3571 4093 1537 Size: 275 1196 563 522 4090 Fragments arranged by size: 4090 1196 563 522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2219 3776 4576 1353 Size: 866 1557 800 3423 Fragments arranged by size: 3423 1557 866 800 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5233 5607 5753 387 Size: 1064 138 3644 374 146 1280 Fragments arranged by size: 3644 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2826 2826 Size: 6646 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2102 2137 2330 2738 3836 3887 Size: 393 421 49 35 193 408 1098 51 Cuts at: 3887 4709 5306 6093 6361 1239 Size: 822 597 787 268 1524 Fragments arranged by size: 1524 1098 822 787 597 421 408 393 268 193 51 49 35 EcoNI CCTnn'n_nnAGG Cuts at: 3152 3152 Size: 6646 EcoO109I rG'GnC_Cy Cuts at: 2122 2349 2804 2953 3484 3937 2122 Size: 227 455 149 531 453 4831 Fragments arranged by size: 4831 531 455 453 227 149 EcoRI G'AATT_C Cuts at: 2647 3955 4333 2647 Size: 1308 378 4960 Fragments arranged by size: 4960 1308 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3067 3067 Size: 6646 FseI GG_CCGG'CC Cuts at: 3843 3843 Size: 6646 FspI TGC'GCA Cuts at: 5152 5152 Size: 6646 HaeII r_GCGC'y Cuts at: 1678 2919 3523 5793 1678 Size: 1241 604 2270 2531 Fragments arranged by size: 2531 2270 1241 604 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2526 2558 2655 2687 3323 Size: 32 135 32 954 32 97 32 636 Cuts at: 3323 3355 3374 3406 3587 3619 5287 5319 5361 Size: 32 19 32 181 32 1668 32 42 Cuts at: 5361 5393 1373 Size: 32 2626 Fragments arranged by size: 2626 1668 954 636 181 135 97 42 32 32 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 2013 3990 4236 669 Size: 988 281 75 1977 246 3079 Fragments arranged by size: 3079 1977 988 281 246 75 HindIII A'AGCT_T Cuts at: 748 748 Size: 6646 HpaI GTT'AAC Cuts at: 4236 4236 Size: 6646 KpnI G_GTAC'C Cuts at: 3971 3971 Size: 6646 MfeI C'AATT_G Cuts at: 4245 4245 Size: 6646 MluI A'CGCG_T Cuts at: 6522 6522 Size: 6646 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2641 2994 5760 5944 1305 Size: 1336 353 2766 184 2007 Fragments arranged by size: 2766 2007 1336 353 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2109 10 Size: 54 1425 343 277 4547 Fragments arranged by size: 4547 1425 343 277 54 MslI CAynn'nnrTG Cuts at: 518 1166 2381 2498 3206 3530 3752 4604 4963 Size: 648 1215 117 708 324 222 852 359 Cuts at: 4963 5122 518 Size: 159 2042 Fragments arranged by size: 2042 1215 852 708 648 359 324 222 159 117 MspA1I CmG'CkG Cuts at: 1606 2758 2847 3865 3874 4062 4739 5887 6132 Size: 1152 89 1018 9 188 677 1148 245 Cuts at: 6132 1606 Size: 2120 Fragments arranged by size: 2120 1152 1148 1018 677 245 188 89 9 NaeI GCC'GGC Cuts at: 2990 3841 2990 Size: 851 5795 NcoI C'CATG_G Cuts at: 513 1065 2257 3302 3672 513 Size: 552 1192 1045 370 3487 Fragments arranged by size: 3487 1192 1045 552 370 NdeI CA'TA_TG Cuts at: 387 387 Size: 6646 NgoMIV G'CCGG_C Cuts at: 2988 3839 2988 Size: 851 5795 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6646 NotI GC'GGCC_GC Cuts at: 4093 4093 Size: 6646 NspI r_CATG'y Cuts at: 1132 1229 3832 4004 5549 1132 Size: 97 2603 172 1545 2229 Fragments arranged by size: 2603 2229 1545 172 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6646 PciI A'CATG_T Cuts at: 5545 5545 Size: 6646 PflMI CCAn_nnn'nTGG Cuts at: 1108 2428 2614 2800 1108 Size: 1320 186 186 4954 Fragments arranged by size: 4954 1320 186 186 PfoI T'CCnGG_A Cuts at: 3702 3702 Size: 6646 PmeI GTTT'AAAC Cuts at: 3950 3950 Size: 6646 PmlI CAC'GTG Cuts at: 2343 2343 Size: 6646 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4715 4747 6228 6260 4715 Size: 32 1481 32 5101 Fragments arranged by size: 5101 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2349 2953 3937 2349 Size: 604 984 5058 Fragments arranged by size: 5058 984 604 PshAI GACnn'nnGTC Cuts at: 1613 2438 2958 1613 Size: 825 520 5301 Fragments arranged by size: 5301 825 520 PsiI TTA'TAA Cuts at: 4216 4216 Size: 6646 PspOMI G'GGCC_C Cuts at: 2235 3856 2235 Size: 1621 5025 PspXI vC'TCGA_Gb Cuts at: 2765 2765 Size: 6646 PstI C_TGCA'G Cuts at: 830 2171 2897 3998 830 Size: 1341 726 1101 3478 Fragments arranged by size: 3478 1341 1101 726 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6646 PvuII CAG'CTG Cuts at: 2758 3865 3874 2758 Size: 1107 9 5530 Fragments arranged by size: 5530 1107 9 SacI G_AGCT'C Cuts at: 2828 2828 Size: 6646 SalI G'TCGA_C Cuts at: 1936 3988 1936 Size: 2052 4594 SapI GCTCTTCn'nnn_ Cuts at: 2219 2219 Size: 6646 SbfI CC_TGCA'GG Cuts at: 3998 3998 Size: 6646 ScaI AGT'ACT Cuts at: 2192 4894 6606 2192 Size: 2702 1712 2232 Fragments arranged by size: 2702 2232 1712 SfcI C'TryA_G Cuts at: 826 1045 2167 2893 3994 5129 5810 6001 826 Size: 219 1122 726 1101 1135 681 191 1471 Fragments arranged by size: 1471 1135 1122 1101 726 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6646 SmaI CCC'GGG Cuts at: 1872 3973 1872 Size: 2101 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2765 2884 3149 3194 3497 4750 Size: 197 926 822 119 265 45 303 1253 Cuts at: 4750 5651 5913 820 Size: 901 262 1553 Fragments arranged by size: 1553 1253 926 901 822 303 265 262 197 119 45 SnaBI TAC'GTA Cuts at: 493 4437 493 Size: 3944 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6646 SphI G_CATG'C Cuts at: 1132 3832 4004 1132 Size: 2700 172 3774 Fragments arranged by size: 3774 2700 172 SspI AAT'ATT Cuts at: 5 52 4570 5 Size: 47 4518 2081 Fragments arranged by size: 4518 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6646 StyI C'CwwG_G Cuts at: 513 1065 2257 2280 3302 3327 3672 4085 513 Size: 552 1192 23 1022 25 345 413 3074 Fragments arranged by size: 3074 1192 1022 552 413 345 25 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2250 4673 4690 4843 5028 5473 325 Size: 1255 670 2423 17 153 185 445 1498 Fragments arranged by size: 2423 1498 1255 670 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2190 2400 3246 Size: 274 80 33 51 1443 213 210 846 Cuts at: 3246 4892 6604 96 Size: 1646 1712 138 Fragments arranged by size: 1712 1646 1443 846 274 213 210 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2583 2615 6606 6638 2583 Size: 32 3991 32 2591 Fragments arranged by size: 3991 2591 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6646 XbaI T'CTAG_A Cuts at: 3982 3982 Size: 6646 XhoI C'TCGA_G Cuts at: 1943 2765 1943 Size: 822 5824 XmaI C'CCGG_G Cuts at: 1870 3971 1870 Size: 2101 4545 XmnI GAAnn'nnTTC Cuts at: 4775 4775 Size: 6646 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4454 276 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I EcoRV FspAI KasI NarI NruI NsiI PacI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI