Restriction map for
pFN21AB6008
(Linear) (Six-base) MAPSORT of: KIBB6008.seq Check: 1967 from: 1 to: 8439
KIBB6008.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1114 3142 3271 3459 7084 7279 7363 7812
Size: 1114 2028 129 188 3625 195 84 449
Cuts at: 7812 7876 8149 8337 8439
Size: 64 273 188 102
Fragments arranged by size:
3625 2028 1114 449 273 195 188 188
129 102 84 64
AatII G_ACGT'C
Cuts at: 0 1412 1765 3200 5279 5408 5675 8439
Size: 1412 353 1435 2079 129 267 2764
Fragments arranged by size:
2764 2079 1435 1412 353 267 129
AccI GT'mk_AC
Cuts at: 0 4811 6623 8439
Size: 4811 1812 1816
Fragments arranged by size:
4811 1816 1812
Acc65I G'GTAC_C
Cuts at: 0 298 4748 8439
Size: 298 4450 3691
Fragments arranged by size:
4450 3691 298
AclI AA'CG_TT
Cuts at: 0 6122 8439
Size: 6122 2317
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 703 1200 1393 2369 2971 3599 3907
Size: 527 176 497 193 976 602 628 308
Cuts at: 3907 4089 4145 4231 4406 4638 5330 5755 6770
Size: 182 56 86 175 232 692 425 1015
Cuts at: 6770 8002 8243 8439
Size: 1232 241 196
Fragments arranged by size:
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425 308 241 232 196 193 182 176
175 86 56
AfeI AGC'GCT
Cuts at: 0 1066 8439
Size: 1066 7373
AflIII A'CryG_T
Cuts at: 0 1832 3326 3466 6907 8152 8439
Size: 1832 1494 140 3441 1245 287
Fragments arranged by size:
3441 1832 1494 1245 287 140
AhdI GACnn_n'nnGTC
Cuts at: 0 6245 7530 8439
Size: 6245 1285 909
Fragments arranged by size:
6245 1285 909
AleI CACnn'nnGTG
Cuts at: 0 694 3329 5185 8439
Size: 694 2635 1856 3254
Fragments arranged by size:
3254 2635 1856 694
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 207 4159 5070 6969 8439
Size: 207 3952 911 1899 1470
Fragments arranged by size:
3952 1899 1470 911 207
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4483 4515 7013 7045 8439
Size: 4483 32 2498 32 1394
Fragments arranged by size:
4483 2498 1394 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 681 932 942 1008 1236 1683 4209 4914
Size: 681 251 10 66 228 447 2526 705
Cuts at: 4914 5067 5434 5949 6801 8439
Size: 153 367 515 852 1638
Fragments arranged by size:
2526 1638 852 705 681 515 447 367
251 228 153 66 10
ApaI G_GGCC'C
Cuts at: 0 4253 4438 4469 4894 7772 8439
Size: 4253 185 31 425 2878 667
Fragments arranged by size:
4253 2878 667 425 185 31
ApaLI G'TGCA_C
Cuts at: 0 1317 3446 5847 7350 7797 8439
Size: 1317 2129 2401 1503 447 642
Fragments arranged by size:
2401 2129 1503 1317 642 447
ApoI r'AATT_y
Cuts at: 0 268 2991 5892 8129 8439
Size: 268 2723 2901 2237 310
Fragments arranged by size:
2901 2723 2237 310 268
AvaI C'yCGr_G
Cuts at: 0 727 1091 1244 1631 2356 2433 2656 3229
Size: 727 364 153 387 725 77 223 573
Cuts at: 3229 4437 4973 5665 6961 7674 7771 8439
Size: 1208 536 692 1296 713 97 668
Fragments arranged by size:
1296 1208 727 725 713 692 668 573
536 387 364 223 153 97 77
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 156 189 8439
Size: 156 33 8250
Fragments arranged by size:
8250 156 33
BamHI G'GATC_C
Cuts at: 0 2717 8439
Size: 2717 5722
BanI G'GyrC_C
Cuts at: 0 298 1078 1870 3732 4748 4797 4875 5359
Size: 298 780 792 1862 1016 49 78 484
Cuts at: 5359 5971 6130 7478 8137 8439
Size: 612 159 1348 659 302
Fragments arranged by size:
1862 1348 1016 792 780 659 612 484
302 298 159 78 49
BanII G_rGCy'C
Cuts at: 0 576 1631 2078 2357 2642 3085 3760 3992
Size: 576 1055 447 279 285 443 675 232
Cuts at: 3992 4169 4253 4438 4445 4469 4894 6676 7772
Size: 177 84 185 7 24 425 1782 1096
Cuts at: 7772 8342 8439
Size: 570 97
Fragments arranged by size:
1782 1096 1055 675 576 570 447 443
425 285 279 232 185 177 97 84
24 7
BbeI G_GCGC'C
Cuts at: 0 1874 4801 8439
Size: 1874 2927 3638
Fragments arranged by size:
3638 2927 1874
BbsI GAAGACnn'nnnn_
Cuts at: 0 760 1561 2162 3053 3192 3587 3828 5570
Size: 760 801 601 891 139 395 241 1742
Cuts at: 5570 7159 7526 7783 7867 8439
Size: 1589 367 257 84 572
Fragments arranged by size:
1742 1589 891 801 760 601 572 395
367 257 241 139 84
BbvCI CC'TCA_GC
Cuts at: 0 1474 2257 5767 6292 6850 7701 8108 8439
Size: 1474 783 3510 525 558 851 407 331
Fragments arranged by size:
3510 1474 851 783 558 525 407 331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 93 127 3292 3326 8164 8198 8439
Size: 93 34 3165 34 4838 34 241
Fragments arranged by size:
4838 3165 241 93 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 157 8439
Size: 157 8282
BfrBI ATG'CAT
Cuts at: 0 5355 6141 8439
Size: 5355 786 2298
Fragments arranged by size:
5355 2298 786
BglI GCCn_nnn'nGGC
Cuts at: 0 1868 1961 2054 2435 5380 5528 7676 8439
Size: 1868 93 93 381 2945 148 2148 763
Fragments arranged by size:
2945 2148 1868 763 381 148 93 93
BglII A'GATC_T
Cuts at: 0 2141 6836 8439
Size: 2141 4695 1603
Fragments arranged by size:
4695 2141 1603
BlpI GC'TnA_GC
Cuts at: 0 4318 5263 6214 7687 8439
Size: 4318 945 951 1473 752
Fragments arranged by size:
4318 1473 951 945 752
Bme1580I G_kGCm'C
Cuts at: 0 828 886 1321 2108 2122 3336 3379 3450
Size: 828 58 435 787 14 1214 43 71
Cuts at: 3450 4253 4438 4469 4880 4894 5782 5851 6033
Size: 803 185 31 411 14 888 69 182
Cuts at: 6033 7354 7772 7801 8439
Size: 1321 418 29 638
Fragments arranged by size:
1321 1214 888 828 803 787 638 435
418 411 185 182 71 69 58 43
31 29 14 14
BmgBI CAC'GTC
Cuts at: 0 1833 4608 5073 5226 6365 8439
Size: 1833 2775 465 153 1139 2074
Fragments arranged by size:
2775 2074 1833 1139 465 153
BmrI ACTGGGnnnn_n'
Cuts at: 0 871 1512 2109 3641 4742 6979 7000 7152
Size: 871 641 597 1532 1101 2237 21 152
Cuts at: 7152 7392 7524 7551 8439
Size: 240 132 27 888
Fragments arranged by size:
2237 1532 1101 888 871 641 597 240
152 132 27 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 265 3801 8439
Size: 265 3536 4638
Fragments arranged by size:
4638 3536 265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 826 2071 2410 2534 3378 3518 3840
Size: 802 24 1245 339 124 844 140 322
Cuts at: 3840 4357 4880 4936 5082 5536 5971 6005 6400
Size: 517 523 56 146 454 435 34 395
Cuts at: 6400 6449 6597 6698 7015 7675 7870 7984 8439
Size: 49 148 101 317 660 195 114 455
Fragments arranged by size:
1245 844 802 660 523 517 455 454
435 395 339 322 317 195 148 146
140 124 114 101 56 49 34 24
Bpu10I CC'TnA_GC
Cuts at: 0 1474 2257 2932 3479 3656 5767 6292 6850
Size: 1474 783 675 547 177 2111 525 558
Cuts at: 6850 7701 8108 8439
Size: 851 407 331
Fragments arranged by size:
2111 1474 851 783 675 558 547 525
407 331 177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3976 4249 8439
Size: 3976 273 4190
Fragments arranged by size:
4190 3976 273
BsaI GGTCTCn'nnnn_
Cuts at: 0 5806 6090 8439
Size: 5806 284 2349
Fragments arranged by size:
5806 2349 284
BsaAI yAC'GTr
Cuts at: 0 2597 3327 3467 6099 6845 6908 8439
Size: 2597 730 140 2632 746 63 1531
Fragments arranged by size:
2632 2597 1531 746 730 140 63
BsaHI Gr'CG_yC
Cuts at: 0 1409 1762 1871 3197 4798 5276 5405 5672
Size: 1409 353 109 1326 1601 478 129 267
Cuts at: 5672 8439
Size: 2767
Fragments arranged by size:
2767 1601 1409 1326 478 353 267 129
109
BsaWI w'CCGG_w
Cuts at: 0 388 1057 2558 3998 4822 5011 8439
Size: 388 669 1501 1440 824 189 3428
Fragments arranged by size:
3428 1501 1440 824 669 388 189
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1252 1282 1822 1852 2507 2537 2653 2683
Size: 1252 30 540 30 655 30 116 30
Cuts at: 2683 2959 2989 3250 3280 3400 3430 3713 3743
Size: 276 30 261 30 120 30 283 30
Cuts at: 3743 3955 3985 6168 6198 6373 6403 6502 6532
Size: 212 30 2183 30 175 30 99 30
Cuts at: 6532 6658 6676 6688 6706 7013 7043 8152 8182
Size: 126 18 12 18 307 30 1109 30
Cuts at: 8182 8296 8326 8439
Size: 114 30 113
Fragments arranged by size:
2183 1252 1109 655 540 307 283 276
261 212 175 126 120 116 114 113
99 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 602 627 1756 2807 3406 3843 4385 4579
Size: 602 25 1129 1051 599 437 542 194
Cuts at: 4579 5085 6397 6415 7108 7348 7627 7976 8057
Size: 506 1312 18 693 240 279 349 81
Cuts at: 8057 8173 8439
Size: 116 266
Fragments arranged by size:
1312 1129 1051 693 602 599 542 506
437 349 279 266 240 194 116 81
25 18
BseYI C'CCAG_C
Cuts at: 0 370 616 772 2895 3335 5106 5299 5501
Size: 370 246 156 2123 440 1771 193 202
Cuts at: 5501 5523 7649 8439
Size: 22 2126 790
Fragments arranged by size:
2126 2123 1771 790 440 370 246 202
193 156 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 891 928 1336 1648 2556 2701 3520
Size: 86 805 37 408 312 908 145 819
Cuts at: 3520 4927 5030 6031 6367 6744 6825 6855 7080
Size: 1407 103 1001 336 377 81 30 225
Cuts at: 7080 7341 7840 7875 8320 8341 8346 8439
Size: 261 499 35 445 21 5 93
Fragments arranged by size:
1407 1001 908 819 805 499 445 408
377 336 312 261 225 145 103 93
86 81 37 35 30 21 5
BsiEI CG_ry'CG
Cuts at: 0 1854 2609 4009 4119 7435 8439
Size: 1854 755 1400 110 3316 1004
Fragments arranged by size:
3316 1854 1400 1004 755 110
BsiHKAI G_wGCw'C
Cuts at: 0 691 1036 1321 1840 2602 3085 3450 3662
Size: 691 345 285 519 762 483 365 212
Cuts at: 3662 5617 5851 6676 7354 7405 7801 8314 8439
Size: 1955 234 825 678 51 396 513 125
Fragments arranged by size:
1955 825 762 691 678 519 513 483
396 365 345 285 234 212 125 51
BsmI GAATG_Cn'
Cuts at: 0 1053 2038 6451 6948 8439
Size: 1053 985 4413 497 1491
Fragments arranged by size:
4413 1491 1053 985 497
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4957 5182 5412 7644 7987 8439
Size: 4957 225 230 2232 343 452
Fragments arranged by size:
4957 2232 452 343 230 225
Bsp1286I G_dGCh'C
Cuts at: 0 576 691 828 886 1036 1321 1631 1840
Size: 576 115 137 58 150 285 310 209
Cuts at: 1840 2078 2108 2122 2357 2602 2642 3085 3336
Size: 238 30 14 235 245 40 443 251
Cuts at: 3336 3379 3450 3662 3760 3992 4169 4253 4438
Size: 43 71 212 98 232 177 84 185
Cuts at: 4438 4445 4469 4880 4894 5617 5782 5851 6033
Size: 7 24 411 14 723 165 69 182
Cuts at: 6033 6676 7354 7405 7772 7801 8314 8342 8439
Size: 643 678 51 367 29 513 28 97
Fragments arranged by size:
723 678 643 576 513 443 411 367
310 285 251 245 238 235 232 212
209 185 182 177 165 150 137 115
98 97 84 71 69 58 51 43
40 30 29 28 24 14 14 7
BspEI T'CCGG_A
Cuts at: 0 1057 5011 8439
Size: 1057 3954 3428
Fragments arranged by size:
3954 3428 1057
BspHI T'CATG_A
Cuts at: 0 3149 7973 8439
Size: 3149 4824 466
Fragments arranged by size:
4824 3149 466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 74 481 940 1114 1244 1499 1567 1691
Size: 74 407 459 174 130 255 68 124
Cuts at: 1691 1820 1982 2011 2348 3142 3271 3459 3574
Size: 129 162 29 337 794 129 188 115
Cuts at: 3574 5842 7007 7084 7279 7363 7812 7813 7876
Size: 2268 1165 77 195 84 449 1 63
Cuts at: 7876 8149 8337 8439
Size: 273 188 102
Fragments arranged by size:
2268 1165 794 459 449 407 337 273
255 195 188 188 174 162 130 129
129 124 115 102 84 77 74 68
63 29 1
BsrBI CCG'CTC
Cuts at: 0 1325 2009 3915 8439
Size: 1325 684 1906 4524
Fragments arranged by size:
4524 1906 1325 684
BsrDI GCAATG_nn'
Cuts at: 0 1146 2043 3308 4025 8439
Size: 1146 897 1265 717 4414
Fragments arranged by size:
4414 1265 1146 897 717
BsrFI r'CCGG_y
Cuts at: 0 2876 3608 8439
Size: 2876 732 4831
Fragments arranged by size:
4831 2876 732
BsrGI T'GTAC_A
Cuts at: 0 149 8439
Size: 149 8290
BstAPI GCAn_nnn'nTGC
Cuts at: 0 626 1106 1390 4692 5437 6767 7137 7805
Size: 626 480 284 3302 745 1330 370 668
Cuts at: 7805 7877 8439
Size: 72 562
Fragments arranged by size:
3302 1330 745 668 626 562 480 370
284 72
BstEII G'GTnAC_C
Cuts at: 0 1432 2772 3351 4635 4783 4818 5580 8439
Size: 1432 1340 579 1284 148 35 762 2859
Fragments arranged by size:
2859 1432 1340 1284 762 579 148 35
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2784 4488 4887 5719 8349 8439
Size: 2784 1704 399 832 2630 90
Fragments arranged by size:
2784 2630 1704 832 399 90
BstYI r'GATC_y
Cuts at: 0 1130 1223 2141 2717 4271 4700 5027 5510
Size: 1130 93 918 576 1554 429 327 483
Cuts at: 5510 6257 6836 8439
Size: 747 579 1603
Fragments arranged by size:
1603 1554 1130 918 747 579 576 483
429 327 93
Bsu36I CC'TnA_GG
Cuts at: 0 995 3621 8439
Size: 995 2626 4818
Fragments arranged by size:
4818 2626 995
BtgI C'CryG_G
Cuts at: 0 92 1394 1512 1889 2309 2912 2942 3950
Size: 92 1302 118 377 420 603 30 1008
Cuts at: 3950 4481 4640 5230 5576 6890 7516 8402 8439
Size: 531 159 590 346 1314 626 886 37
Fragments arranged by size:
1314 1302 1008 886 626 603 590 531
420 377 346 159 118 92 37 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 984 2260 4122 7358 8439
Size: 984 1276 1862 3236 1081
Fragments arranged by size:
3236 1862 1276 1081 984
BtsI GCAGTG_nn'
Cuts at: 0 588 676 913 1984 3094 3508 3525 3660
Size: 588 88 237 1071 1110 414 17 135
Cuts at: 3660 4329 6394 6411 8180 8358 8439
Size: 669 2065 17 1769 178 81
Fragments arranged by size:
2065 1769 1110 1071 669 588 414 237
178 135 88 81 17 17
ClaI AT'CG_AT
Cuts at: 0 419 7790 8439
Size: 419 7371 649
Fragments arranged by size:
7371 649 419
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 5366 5401 7233 7268 8439
Size: 5366 35 1832 35 1171
Fragments arranged by size:
5366 1832 1171 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1040 2183 3374 5703 5874 6767 8439
Size: 1040 1143 1191 2329 171 893 1672
Fragments arranged by size:
2329 1672 1191 1143 1040 893 171
DrdI GACnn_nn'nnGTC
Cuts at: 0 322 8439
Size: 322 8117
EaeI y'GGCC_r
Cuts at: 0 410 1401 1509 1851 1877 2874 3365 3606
Size: 410 991 108 342 26 997 491 241
Cuts at: 3606 3947 4154 5721 6352 6893 8439
Size: 341 207 1567 631 541 1546
Fragments arranged by size:
1567 1546 997 991 631 541 491 410
342 341 241 207 108 26
EagI C'GGCC_G
Cuts at: 0 1851 8439
Size: 1851 6588
EarI CTCTTCn'nnn_
Cuts at: 0 679 2824 5125 5653 5731 5804 6746 7537
Size: 679 2145 2301 528 78 73 942 791
Cuts at: 7537 8056 8439
Size: 519 383
Fragments arranged by size:
2301 2145 942 791 679 528 519 383
78 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1639 4264 6873 6925 8439
Size: 1639 2625 2609 52 1514
Fragments arranged by size:
2625 2609 1639 1514 52
EcoICRI GAG'CTC
Cuts at: 0 3083 6674 8439
Size: 3083 3591 1765
Fragments arranged by size:
3591 3083 1765
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 703 802 826 1200 1393 2071 2369
Size: 527 176 99 24 374 193 678 298
Cuts at: 2369 2410 2534 2971 3378 3518 3599 3840 3907
Size: 41 124 437 407 140 81 241 67
Cuts at: 3907 4089 4145 4231 4357 4406 4638 4880 4936
Size: 182 56 86 126 49 232 242 56
Cuts at: 4936 5082 5330 5536 5755 5971 6005 6400 6449
Size: 146 248 206 219 216 34 395 49
Cuts at: 6449 6597 6698 6770 7015 7675 7870 7984 8002
Size: 148 101 72 245 660 195 114 18
Cuts at: 8002 8243 8439
Size: 241 196
Fragments arranged by size:
678 660 527 437 407 395 374 298
248 245 242 241 241 232 219 216
206 196 195 193 182 176 148 146
140 126 124 114 101 99 86 81
72 67 56 56 49 49 41 34
24 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2785 4951 8439
Size: 2785 2166 3488
Fragments arranged by size:
3488 2785 2166
EcoO109I rG'GnC_Cy
Cuts at: 0 56 533 657 929 1488 1680 1985 2708
Size: 56 477 124 272 559 192 305 723
Cuts at: 2708 4206 4250 4293 4465 4891 5170 5296 5946
Size: 1498 44 43 172 426 279 126 650
Cuts at: 5946 6420 6456 6813 7659 8439
Size: 474 36 357 846 780
Fragments arranged by size:
1498 846 780 723 650 559 477 474
426 357 305 279 272 192 172 126
124 56 44 43 36
EcoRV GAT'ATC
Cuts at: 0 8238 8439
Size: 8238 201
FspI TGC'GCA
Cuts at: 0 3922 8439
Size: 3922 4517
HaeII r_GCGC'y
Cuts at: 0 1068 1874 3435 4801 8439
Size: 1068 806 1561 1366 3638
Fragments arranged by size:
3638 1561 1366 1068 806
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1822 1854 2149 2181 3250 3282 3838 3870
Size: 1822 32 295 32 1069 32 556 32
Cuts at: 3870 4651 4683 5080 5112 6231 6263 6676 6708
Size: 781 32 397 32 1119 32 413 32
Cuts at: 6708 7516 7548 7955 7987 8296 8328 8439
Size: 808 32 407 32 309 32 111
Fragments arranged by size:
1822 1119 1069 808 781 556 413 407
397 309 295 111 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 129 3573 3690 5751 6120 6573 6867 7197
Size: 129 3444 117 2061 369 453 294 330
Cuts at: 7197 8439
Size: 1242
Fragments arranged by size:
3444 2061 1242 453 369 330 294 129
117
HindIII A'AGCT_T
Cuts at: 0 2235 8439
Size: 2235 6204
KasI G'GCGC_C
Cuts at: 0 1870 4797 8439
Size: 1870 2927 3642
Fragments arranged by size:
3642 2927 1870
KpnI G_GTAC'C
Cuts at: 0 302 4752 8439
Size: 302 4450 3687
Fragments arranged by size:
4450 3687 302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 5474 5561 6228 6532 6710 8350 8439
Size: 5474 87 667 304 178 1640 89
Fragments arranged by size:
5474 1640 667 304 178 89 87
MscI TGG'CCA
Cuts at: 0 412 1403 1879 4156 5723 8439
Size: 412 991 476 2277 1567 2716
Fragments arranged by size:
2716 2277 1567 991 476 412
MslI CAynn'nnrTG
Cuts at: 0 694 950 2401 2585 2782 2941 3329 3680
Size: 694 256 1451 184 197 159 388 351
Cuts at: 3680 4870 4885 5185 5224 5969 6016 6061 6659
Size: 1190 15 300 39 745 47 45 598
Cuts at: 6659 7363 8347 8439
Size: 704 984 92
Fragments arranged by size:
1451 1190 984 745 704 694 598 388
351 300 256 197 184 159 92 47
45 39 15
MspA1I CmG'CkG
Cuts at: 0 428 707 989 1514 1803 1891 1940 2332
Size: 428 279 282 525 289 88 49 392
Cuts at: 2332 3443 4578 4689 5286 5434 5828 5912 6351
Size: 1111 1135 111 597 148 394 84 439
Cuts at: 6351 6740 6854 7427 7518 7653 7686 7811 8439
Size: 389 114 573 91 135 33 125 628
Fragments arranged by size:
1135 1111 628 597 573 525 439 428
394 392 389 289 282 279 148 135
125 114 111 91 88 84 49 33
NaeI GCC'GGC
Cuts at: 0 3610 8439
Size: 3610 4829
NarI GG'CG_CC
Cuts at: 0 1871 4798 8439
Size: 1871 2927 3641
Fragments arranged by size:
3641 2927 1871
NcoI C'CATG_G
Cuts at: 0 1394 2309 4481 5230 5576 8402 8439
Size: 1394 915 2172 749 346 2826 37
Fragments arranged by size:
2826 2172 1394 915 749 346 37
NgoMIV G'CCGG_C
Cuts at: 0 3608 8439
Size: 3608 4831
NsiI A_TGCA'T
Cuts at: 0 5357 6143 8439
Size: 5357 786 2296
Fragments arranged by size:
5357 2296 786
NspI r_CATG'y
Cuts at: 0 150 7749 8156 8439
Size: 150 7599 407 283
Fragments arranged by size:
7599 407 283 150
PasI CC'CwG_GG
Cuts at: 0 2071 2704 4461 7884 8439
Size: 2071 633 1757 3423 555
Fragments arranged by size:
3423 2071 1757 633 555
PciI A'CATG_T
Cuts at: 0 8152 8439
Size: 8152 287
PflMI CCAn_nnn'nTGG
Cuts at: 0 569 717 5067 6317 6801 8439
Size: 569 148 4350 1250 484 1638
Fragments arranged by size:
4350 1638 1250 569 484 148
PfoI T'CCnGG_A
Cuts at: 0 803 1007 3070 3203 3404 3545 4180 4505
Size: 803 204 2063 133 201 141 635 325
Cuts at: 4505 4952 5084 5243 6377 6800 6815 7537 8000
Size: 447 132 159 1134 423 15 722 463
Cuts at: 8000 8439
Size: 439
Fragments arranged by size:
2063 1134 803 722 635 463 447 439
423 325 204 201 159 141 133 132
15
PmlI CAC'GTG
Cuts at: 0 2597 3327 3467 6845 6908 8439
Size: 2597 730 140 3378 63 1531
Fragments arranged by size:
3378 2597 1531 730 140 63
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 7013 7045 8439
Size: 7013 32 1394
Fragments arranged by size:
7013 1394 32
PpuMI rG'GwC_Cy
Cuts at: 0 56 533 929 1488 1680 1985 2708 5946
Size: 56 477 396 559 192 305 723 3238
Cuts at: 5946 6420 6456 6813 7659 8439
Size: 474 36 357 846 780
Fragments arranged by size:
3238 846 780 723 559 477 474 396
357 305 192 56 36
PshAI GACnn'nnGTC
Cuts at: 0 7427 8439
Size: 7427 1012
PspOMI G'GGCC_C
Cuts at: 0 4249 4434 4465 4890 7768 8439
Size: 4249 185 31 425 2878 671
Fragments arranged by size:
4249 2878 671 425 185 31
PspXI vC'TCGA_Gb
Cuts at: 0 2356 8439
Size: 2356 6083
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1810 1842 8439
Size: 1810 32 6597
Fragments arranged by size:
6597 1810 32
PstI C_TGCA'G
Cuts at: 0 172 988 1138 1496 1688 1979 3817 4622
Size: 172 816 150 358 192 291 1838 805
Cuts at: 4622 4688 5000 5027 5059 5201 5980 6212 7087
Size: 66 312 27 32 142 779 232 875
Cuts at: 7087 8195 8439
Size: 1108 244
Fragments arranged by size:
1838 1108 875 816 805 779 358 312
291 244 232 192 172 150 142 66
32 27
PvuI CG_AT'CG
Cuts at: 0 2609 8439
Size: 2609 5830
PvuII CAG'CTG
Cuts at: 0 989 3443 5434 5912 6740 7427 7653 7686
Size: 989 2454 1991 478 828 687 226 33
Cuts at: 7686 8439
Size: 753
Fragments arranged by size:
2454 1991 989 828 753 687 478 226
33
SacI G_AGCT'C
Cuts at: 0 3085 6676 8439
Size: 3085 3591 1763
Fragments arranged by size:
3591 3085 1763
SacII CC_GC'GG
Cuts at: 0 1515 1892 7519 8439
Size: 1515 377 5627 920
Fragments arranged by size:
5627 1515 920 377
SanDI GG'GwC_CC
Cuts at: 0 56 1985 6813 8439
Size: 56 1929 4828 1626
Fragments arranged by size:
4828 1929 1626 56
SapI GCTCTTCn'nnn_
Cuts at: 0 679 8439
Size: 679 7760
SbfI CC_TGCA'GG
Cuts at: 0 7087 8439
Size: 7087 1352
ScaI AGT'ACT
Cuts at: 0 4624 8050 8439
Size: 4624 3426 389
Fragments arranged by size:
4624 3426 389
SexAI A'CCwGG_T
Cuts at: 0 1729 3830 4805 4988 8439
Size: 1729 2101 975 183 3451
Fragments arranged by size:
3451 2101 1729 975 183
SfcI C'TryA_G
Cuts at: 0 168 984 1134 1492 1684 1975 3177 3813
Size: 168 816 150 358 192 291 1202 636
Cuts at: 3813 4618 4684 4996 5023 5055 5197 5976 6208
Size: 805 66 312 27 32 142 779 232
Cuts at: 6208 7083 8191 8439
Size: 875 1108 248
Fragments arranged by size:
1202 1108 875 816 805 779 636 358
312 291 248 232 192 168 150 142
66 32 27
SfoI GGC'GCC
Cuts at: 0 1872 4799 8439
Size: 1872 2927 3640
Fragments arranged by size:
3640 2927 1872
SmaI CCC'GGG
Cuts at: 0 1246 1633 2435 2658 4439 5667 7773 8439
Size: 1246 387 802 223 1781 1228 2106 666
Fragments arranged by size:
2106 1781 1246 1228 802 666 387 223
SmlI C'TyrA_G
Cuts at: 0 1091 2356 3991 4264 8439
Size: 1091 1265 1635 273 4175
Fragments arranged by size:
4175 1635 1265 1091 273
SphI G_CATG'C
Cuts at: 0 7749 8439
Size: 7749 690
StuI AGG'CCT
Cuts at: 0 397 1019 1780 2044 3725 3892 4309 5772
Size: 397 622 761 264 1681 167 417 1463
Cuts at: 5772 6757 7741 8439
Size: 985 984 698
Fragments arranged by size:
1681 1463 985 984 761 698 622 417
397 264 167
StyI C'CwwG_G
Cuts at: 0 1394 2108 2309 4481 5141 5230 5318 5576
Size: 1394 714 201 2172 660 89 88 258
Cuts at: 5576 5585 6162 7218 8402 8439
Size: 9 577 1056 1184 37
Fragments arranged by size:
2172 1394 1184 1056 714 660 577 258
201 89 88 37 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 871 6091 7421 8439
Size: 871 5220 1330 1018
Fragments arranged by size:
5220 1330 1018 871
TatI w'GTAC_w
Cuts at: 0 149 4622 8048 8276 8439
Size: 149 4473 3426 228 163
Fragments arranged by size:
4473 3426 228 163 149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 48 80 1026 1058 1252 1284 2507 2539
Size: 48 32 946 32 194 32 1223 32
Cuts at: 2539 2548 2580 2959 2991 3627 3659 3713 3745
Size: 9 32 379 32 636 32 54 32
Cuts at: 3745 3955 3987 4812 4844 6168 6200 6373 6405
Size: 210 32 825 32 1324 32 173 32
Cuts at: 6405 6502 6534 7260 7292 8152 8184 8439
Size: 97 32 726 32 860 32 255
Fragments arranged by size:
1324 1223 946 860 825 726 636 379
255 210 194 173 97 54 48 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 9
Tth111I GACn'n_nGTC
Cuts at: 0 124 2251 3832 8439
Size: 124 2127 1581 4607
Fragments arranged by size:
4607 2127 1581 124
XbaI T'CTAG_A
Cuts at: 0 357 8439
Size: 357 8082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2316 2610 3771 4734 5583 6637 7314 8439
Size: 2316 294 1161 963 849 1054 677 1125
Fragments arranged by size:
2316 1161 1125 1054 963 849 677 294
XhoI C'TCGA_G
Cuts at: 0 1091 2356 8439
Size: 1091 1265 6083
Fragments arranged by size:
6083 1265 1091
XmaI C'CCGG_G
Cuts at: 0 1244 1631 2433 2656 4437 5665 7771 8439
Size: 1244 387 802 223 1781 1228 2106 668
Fragments arranged by size:
2106 1781 1244 1228 802 668 387 223
XmnI GAAnn'nnTTC
Cuts at: 0 5134 8439
Size: 5134 3305
ZraI GAC'GTC
Cuts at: 0 1410 1763 3198 5277 5406 5673 8439
Size: 1410 353 1435 2079 129 267 2766
Fragments arranged by size:
2766 2079 1435 1410 353 267 129
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII
Hin4I HincII HindIII KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII SacI SacII SanDI
SapI SbfI ScaI SexAI SfcI SfoI SmaI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AscI AseI AsiSI AvrII BclI BmtI
BsaBI BsiWI BssHII BssSI BstBI BstZ17I DraI EcoRI
FalI FseI FspAI HpaI MfeI MluI NdeI NheI
NotI NruI PacI PmeI PsiI RsrII SalI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI