Restriction map for
pFN21AB5637
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# NO CUT ENZYMES ( 99 enzymes) #
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AccI AccII AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AspI AvrII BalI BbeI
BcgI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsgI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
HaeII HpaI KasI KspI MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 203 GACGTC GACGT^C
Acc65I 1086 GGTACC G^GTACC
AcyI 203 GACGTC G[GA]^CG[CT]C
AflIII 460 ACACGT A^C[GA][CT]GT
AhdI 2362 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 267 GGGCCC GGGCC^C
Asp700I 1926 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1086 GGTACC G^GTACC
AspEI 2362 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 876 TGCGCA TGC^GCA
BbvCI 1045 CCTCAGC CCTCAGC(-5/-2)
BclI 2055 TGATCA T^GATCA
BglI 1489 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 203 GACGTC G[GA]^CG[CT]C
BsiEI 190 CGGCCG CG[GA][CT]^CG
BsmI 353 GAATGC GAATGC(1/-1)
BsrBI 1561 GAGCGG CCGCTC(-3/-3)
BssSI 3213 CACGAG CACGAG(-5/-1)
BstAPI 1060 GCAATACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2298 TTCGAA TT^CGAA
DrdI 1713 GACAATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 190 CGGCCG C^GGCCG
Eam1105I 2362 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 190 CGGCCG C^GGCCG
Eco52I 190 CGGCCG C^GGCCG
FbaI 2055 TGATCA T^GATCA
FspI 876 TGCGCA TGC^GCA
HgaI 2997 GACGC GACGC(5/10)
Hin1I 203 GACGTC G[GA]^CG[CT]C
HincII 1639 GTTGAC GT[CT]^[GA]AC
HindII 1639 GTTGAC GT[CT]^[GA]AC
HindIII 2767 AAGCTT A^AGCTT
KpnI 1086 GGTACC GGTAC^C
MfeI 2222 CAATTG C^AATTG
MunI 2222 CAATTG C^AATTG
NdeI 3155 CATATG CA^TATG
NspV 2298 TTCGAA TT^CGAA
PflMI 151 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2704 AGGACCC [GA]G^G[AT]CC[CT]
PstI 803 CTGCAG CTGCA^G
SacI 52 GAGCTC GAGCT^C
ScaI 3111 AGTACT AGT^ACT
SfuI 2298 TTCGAA TT^CGAA
SmaI 45 CCCGGG CCC^GGG
SmlI 78 CTTGAG C^T[CT][GA]AG
StuI 74 AGGCCT AGG^CCT
Van91I 151 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1901 CCATTGTCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 45 CCCGGG C^CCGGG
XmaI 45 CCCGGG C^CCGGG
XmnI 1926 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 52 GAGCTC G[AT]GC[AT]^C
2812 GAGCAC
AvaI 45 CCCGGG C^[CT]CG[GA]G
1889 CCCGAG
BamHI 1413 GGATCC G^GATCC
2737 GGATCC
BanI 1086 GGTACC G^G[CT][GA]CC
1700 GGCACC
BbrPI 461 CACGTG CAC^GTG
2862 CACGTG
BciVI 251 GGATAC GTATCC(6/5)
808 GTATCC
BsaAI 461 CACGTG [CT]AC^GT[GA]
2862 CACGTG
BsaI 285 GAGACC GGTCTC(1/5)
3010 GAGACC
BsiHKAI 52 GAGCTC G[AT]GC[AT]^C
2812 GAGCAC
BsoBI 45 CCCGGG C^[CT]CG[GA]G
1889 CCCGAG
EaeI 190 CGGCCG [CT]^GGCC[GA]
2267 TGGCCG
EcoNI 238 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
241 CCTCCAGGAGG
MslI 978 CACACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2858 CATCCACGTG
MspA1I 2270 CCGCTG C[AC]G^C[GT]G
2724 CCGCTG
NcoI 1534 CCATGG C^CATGG
1865 CCATGG
PleI 1846 GACTC GAGTC(4/5)
2491 GACTC
PmaCI 461 CACGTG CAC^GTG
2862 CACGTG
PmlI 461 CACGTG CAC^GTG
2862 CACGTG
SphI 1115 GCATGC GCATG^C
2196 GCATGC
Tsp45I 364 GTGAC ^GT[GC]AC
2924 GTGAC
TspRI 113 CAGTG CA[GC]TG[ACGT][ACGT]^
2922 CAGTG
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PshBI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp509I
TthHB8I XhoII