Restriction map for
pFN21AB5499
##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AatII Acc65I AclI AhdI AscI
Asp718I AspEI AviII AvrII BamHI
BlnI BsiWI BspLU11I BssHII BssSI
Bst1107I BstBI BstZ17I CpoI DraI
Eam1105I EcoT22I FseI FspI HpaI
KpnI MluI NheI NruI NsiI
NspV PacI PmeI Psp1406I PvuI
RsrII SalI SbfI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 940 GTAGAC GT^[AC][GT]AC
AccIII 3359 TCCGGA T^CCGGA
AflII 1803 CTTAAG C^TTAAG
AgeI 2585 ACCGGT A^CCGGT
AocI 2959 CCTGAGG CC^T[ACGT]AGG
ApaI 508 GGGCCC GGGCC^C
AseI 4549 ATTAAT AT^TAAT
AsnI 4549 ATTAAT AT^TAAT
Asp700I 1152 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1399 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1126 GGCGCC GGCGC^C
BbvCI 4539 CCTCAGC CCTCAGC(-5/-2)
BcgI 4497 CGAGATCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3129 TGATCA T^GATCA
BfrI 1803 CTTAAG C^TTAAG
BsaBI 3432 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3359 TCCGGA T^CCGGA
BsmBI 445 CGTCTC CGTCTC(1/5)
Bsp1407I 1510 TGTACA T^GTACA
BspDI 2500 ATCGAT AT^CGAT
BspEI 3359 TCCGGA T^CCGGA
BspHI 4745 TCATGA T^CATGA
BsrBI 3424 GAGCGG CCGCTC(-3/-3)
BsrGI 1510 TGTACA T^GTACA
BstXI 2739 CCAGGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2959 CCTGAGG CC^T[ACGT]AGG
ClaI 2500 ATCGAT AT^CGAT
DrdI 2147 GACCATACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2959 CCTGAGG CC^T[ACGT]AGG
EcoRI 622 GAATTC G^AATTC
FbaI 3129 TGATCA T^GATCA
KasI 1126 GGCGCC G^GCGCC
MamI 3432 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3359 TCCGGA T^CCGGA
NaeI 24 GCCGGC GCC^GGC
NarI 1126 GGCGCC GG^CGCC
NdeI 932 CATATG CA^TATG
NgoMIV 24 GCCGGC G^CCGGC
NspI 149 GCATGC [GA]CATG^[CT]
PaeR7I 1612 CTCGAG C^TCGAG
PflFI 1399 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2585 ACCGGT A^CCGGT
PshAI 246 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4549 ATTAAT AT^TAAT
RcaI 4745 TCATGA T^CATGA
SacI 2413 GAGCTC GAGCT^C
ScaI 527 AGTACT AGT^ACT
SexAI 346 ACCTGGT A^CC[AT]GGT
SfoI 1126 GGCGCC GGC^GCC
SmaI 38 CCCGGG CCC^GGG
SphI 149 GCATGC GCATG^C
SspBI 1510 TGTACA T^GTACA
Tth111I 1399 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1612 CTCGAG C^TCGAG
XmaCI 38 CCCGGG C^CCGGG
XmaI 38 CCCGGG C^CCGGG
XmnI 1152 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 277 GACGCC G[GA]^CG[CT]C
1126 GGCGCC
AflIII 200 ACACGT A^C[GA][CT]GT
202 ACGTGT
Alw44I 117 GTGCAC G^TGCAC
2373 GTGCAC
ApaLI 117 GTGCAC G^TGCAC
2373 GTGCAC
BbrPI 201 CACGTG CAC^GTG
2728 CACGTG
BciVI 4071 GGATAC GTATCC(6/5)
4581 GGATAC
BfaI 4015 CTAG C^TAG
4532 CTAG
BglII 3797 AGATCT A^GATCT
4365 AGATCT
BlpI 3814 GCTCAGC GC^T[ACGT]AGC
3819 GCTGAGC
Bpu1102I 3814 GCTCAGC GC^T[ACGT]AGC
3819 GCTGAGC
BsaHI 277 GACGCC G[GA]^CG[CT]C
1126 GGCGCC
BsaWI 2585 ACCGGT [AT]^CCGG[AT]
3359 TCCGGA
BstEII 243 GGTGACC G^GT[ACGT]ACC
252 GGTCACC
BstPI 243 GGTGACC G^GT[ACGT]ACC
252 GGTCACC
CelII 3814 GCTCAGC GC^T[ACGT]AGC
3819 GCTGAGC
EcoO65I 243 GGTGACC G^GT[ACGT]ACC
252 GGTCACC
EcoRV 1636 GATATC GAT^ATC
4732 GATATC
Hin1I 277 GACGCC G[GA]^CG[CT]C
1126 GGCGCC
HindIII 422 AAGCTT A^AGCTT
3553 AAGCTT
KspI 83 CCGCGG CCGC^GG
570 CCGCGG
MaeI 4015 CTAG C^TAG
4532 CTAG
MfeI 1416 CAATTG C^AATTG
2652 CAATTG
MunI 1416 CAATTG C^AATTG
2652 CAATTG
NcoI 107 CCATGG C^CATGG
1086 CCATGG
NotI 76 GCGGCCGC GC^GGCCGC
135 GCGGCCGC
PflMI 344 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2811 CCATGCCTTGG
PmaCI 201 CACGTG CAC^GTG
2728 CACGTG
PmlI 201 CACGTG CAC^GTG
2728 CACGTG
PpuMI 3512 GGGACCC [GA]G^G[AT]CC[CT]
3923 AGGACCT
SacII 83 CCGCGG CCGC^GG
570 CCGCGG
SapI 3688 GAAGAGC GCTCTTC(1/4)
1184 GCTCTTC
StuI 605 AGGCCT AGG^CCT
1906 AGGCCT
Van91I 344 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2811 CCATGCCTTGG
XcmI 525 CCAGTACTACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2913 CCACTTTGGGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI Aor51HI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII