(Circular) (Six-base) MAPSORT of: pFN21AB5499.seq Check: 7503 from: 1 to: 9532 pFN21AB5499 9532 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2307 3700 2307 Size: 1393 8139 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7342 278 Size: 53 83 186 936 5806 2468 Fragments arranged by size: 5806 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2946 6875 1937 Size: 1009 3929 4594 Fragments arranged by size: 4594 3929 1009 Acc65I G'GTAC_C Cuts at: 6853 6853 Size: 9532 AclI AA'CG_TT Cuts at: 1563 7659 8032 1563 Size: 6096 373 3063 Fragments arranged by size: 6096 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3339 3627 3862 4574 4956 4967 6072 6222 Size: 1707 288 235 712 382 11 1105 150 Cuts at: 6222 7595 8979 9247 1632 Size: 1373 1384 268 1917 Fragments arranged by size: 1917 1707 1384 1373 1105 712 382 288 268 235 150 11 AfeI AGC'GCT Cuts at: 2150 4904 6307 2150 Size: 2754 1403 5375 Fragments arranged by size: 5375 2754 1403 AflII C'TTAA_G Cuts at: 820 1017 3808 820 Size: 197 2791 6544 Fragments arranged by size: 6544 2791 197 AflIII A'CryG_T Cuts at: 1203 2205 2207 8431 9408 1203 Size: 1002 2 6224 977 1327 Fragments arranged by size: 6224 1327 1002 977 2 AgeI A'CCGG_T Cuts at: 4590 8363 4590 Size: 3773 5759 AhdI GACnn_n'nnGTC Cuts at: 8261 8261 Size: 9532 AleI CACnn'nnGTG Cuts at: 1166 2444 4984 1166 Size: 1278 2540 5714 Fragments arranged by size: 5714 2540 1278 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4660 6729 1184 Size: 3476 2069 3987 Fragments arranged by size: 3987 3476 2069 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2546 2578 2546 Size: 32 9500 AlwNI CAG_nnn'CTG Cuts at: 5740 6238 6344 7275 8847 5740 Size: 498 106 931 1572 6425 Fragments arranged by size: 6425 1572 931 498 106 ApaI G_GGCC'C Cuts at: 2517 2517 Size: 9532 ApaLI G'TGCA_C Cuts at: 2122 4378 7589 8745 2122 Size: 2256 3211 1156 2909 Fragments arranged by size: 3211 2909 2256 1156 ApoI r'AATT_y Cuts at: 1493 2627 2900 3932 5296 6841 7067 7219 9328 Size: 1134 273 1032 1364 1545 226 152 2109 Cuts at: 9328 1493 Size: 1697 Fragments arranged by size: 2109 1697 1545 1364 1134 1032 273 226 152 AseI AT'TA_AT Cuts at: 160 1032 6555 8086 160 Size: 872 5523 1531 1606 Fragments arranged by size: 5523 1606 1531 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9532 AvaI C'yCGr_G Cuts at: 1870 1943 2043 2159 2222 3471 3617 5757 6024 Size: 73 100 116 63 1249 146 2140 267 Cuts at: 6024 6857 1870 Size: 833 4545 Fragments arranged by size: 4545 2140 1249 833 267 146 116 100 73 63 BamHI G'GATC_C Cuts at: 6862 7229 6862 Size: 367 9165 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3131 3147 4298 4397 6623 Size: 325 217 627 1344 16 1151 99 2226 Cuts at: 6623 6853 8308 618 Size: 230 1455 1842 Fragments arranged by size: 2226 1842 1455 1344 1151 627 325 230 217 99 16 BanII G_rGCy'C Cuts at: 1391 2517 3218 4422 4829 5831 6851 1391 Size: 1126 701 1204 407 1002 1020 4072 Fragments arranged by size: 4072 1204 1126 1020 1002 701 407 BbeI G_GCGC'C Cuts at: 3135 3135 Size: 9532 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2514 2954 3789 3868 6791 928 Size: 971 615 440 835 79 2923 3669 Fragments arranged by size: 3669 2923 971 835 615 440 79 BbvCI CC'TCA_GC Cuts at: 6545 6545 Size: 9532 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6491 6525 7723 7757 1903 Size: 34 4554 34 1198 34 3678 Fragments arranged by size: 4554 3678 1198 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6070 6580 7424 8634 6070 Size: 510 844 1210 6968 Fragments arranged by size: 6968 1210 844 510 BclI T'GATC_A Cuts at: 1548 5134 1548 Size: 3586 5946 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2030 2047 2075 Size: 107 122 71 631 605 358 17 28 Cuts at: 2075 2090 3096 4878 5780 5816 6233 6266 6287 Size: 15 1006 1782 902 36 417 33 21 Cuts at: 6287 8143 136 Size: 1856 1525 Fragments arranged by size: 1856 1782 1525 1006 902 631 605 417 358 122 107 71 36 33 28 21 17 15 BglII A'GATC_T Cuts at: 5802 6370 9527 5802 Size: 568 3157 5807 Fragments arranged by size: 5807 3157 568 BlpI GC'TnA_GC Cuts at: 5820 5825 6949 5820 Size: 5 1124 8403 Fragments arranged by size: 8403 1124 5 Bme1580I G_kGCm'C Cuts at: 1790 2104 2126 2517 4382 5413 7593 8749 1790 Size: 314 22 391 1865 1031 2180 1156 2573 Fragments arranged by size: 2573 2180 1865 1156 1031 391 314 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9532 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2661 5196 7263 Size: 416 53 83 399 24 1244 2535 2067 Cuts at: 7263 8221 442 Size: 958 1753 Fragments arranged by size: 2535 2067 1753 1244 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9532 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3643 3933 5165 3643 Size: 290 1232 8010 Fragments arranged by size: 8010 1232 290 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4065 4353 5135 5870 5999 6707 6791 8192 Size: 2826 288 782 735 129 708 84 1401 Cuts at: 8192 1239 Size: 2579 Fragments arranged by size: 2826 2579 1401 782 735 708 288 129 84 Bpu10I CC'TnA_GC Cuts at: 3834 6545 3834 Size: 2711 6821 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2438 2798 3815 5480 6477 7657 8522 8820 2438 Size: 360 1017 1665 997 1180 865 298 3150 Fragments arranged by size: 3150 1665 1180 1017 997 865 360 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3851 4190 4437 8195 882 Size: 617 2352 339 247 3758 2219 Fragments arranged by size: 3758 2352 2219 617 339 247 BsaAI yAC'GTr Cuts at: 493 1204 2208 3974 4735 7323 493 Size: 711 1004 1766 761 2588 2702 Fragments arranged by size: 2702 2588 1766 1004 761 711 BsaBI GATnn'nnATC Cuts at: 5441 9526 5441 Size: 4085 5447 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2283 3132 7339 7721 Size: 53 83 186 936 750 849 4207 382 Cuts at: 7721 8356 275 Size: 635 1451 Fragments arranged by size: 4207 1451 936 849 750 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4590 5364 7965 8363 8637 8784 9195 4590 Size: 774 2601 398 274 147 411 4927 Fragments arranged by size: 4927 2601 774 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2381 2411 2546 2576 2775 Size: 30 165 30 978 30 135 30 199 Cuts at: 2775 2805 4361 4391 1178 Size: 30 1556 30 6319 Fragments arranged by size: 6319 1556 978 199 165 135 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3004 3541 4294 5783 6173 6533 6758 1186 Size: 1818 537 753 1489 390 360 225 3960 Fragments arranged by size: 3960 1818 1489 753 537 390 360 225 BseYI C'CCAG_C Cuts at: 2393 2690 6228 6574 8735 9231 2393 Size: 297 3538 346 2161 496 2694 Fragments arranged by size: 3538 2694 2161 496 346 297 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2258 2399 2405 4057 4285 4513 1163 Size: 573 522 141 6 1652 228 228 6182 Fragments arranged by size: 6182 1652 573 522 228 228 141 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2085 2144 2984 6153 6982 7743 Size: 275 186 84 59 840 3169 829 761 Cuts at: 7743 8771 1540 Size: 1028 2301 Fragments arranged by size: 3169 2301 1028 840 829 761 275 186 84 59 BsiHKAI G_wGCw'C Cuts at: 2126 4033 4382 4422 7593 7678 8749 2126 Size: 1907 349 40 3171 85 1071 2909 Fragments arranged by size: 3171 2909 1907 1071 349 85 40 BsmI GAATG_Cn' Cuts at: 3023 3059 3502 4553 5015 6325 7043 7136 3023 Size: 36 443 1051 462 1310 718 93 5419 Fragments arranged by size: 5419 1310 1051 718 462 443 93 36 BsmBI CGTCTCn'nnnn_ Cuts at: 2456 2456 Size: 9532 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2104 2126 2517 3218 4033 4382 4422 Size: 399 314 22 391 701 815 349 40 Cuts at: 4422 4829 5413 5831 6851 7593 7678 8749 1391 Size: 407 584 418 1020 742 85 1071 2174 Fragments arranged by size: 2174 1071 1020 815 742 701 584 418 407 399 391 349 314 85 40 22 BspEI T'CCGG_A Cuts at: 5364 9195 5364 Size: 3831 5701 BspHI T'CATG_A Cuts at: 6750 7421 9151 6750 Size: 671 1730 7131 Fragments arranged by size: 7131 1730 671 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2307 3700 5652 6887 844 Size: 1463 1393 1952 1235 3489 Fragments arranged by size: 3489 1952 1463 1393 1235 BsrBI CCG'CTC Cuts at: 1394 1823 5431 7419 1394 Size: 429 3608 1988 3507 Fragments arranged by size: 3608 3507 1988 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3043 3681 4189 8027 8201 65 Size: 1183 1795 638 508 3838 174 1396 Fragments arranged by size: 3838 1795 1396 1183 638 508 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2029 2979 4590 4856 5109 5783 8176 Size: 110 219 950 1611 266 253 674 2393 Cuts at: 8176 8363 1700 Size: 187 2869 Fragments arranged by size: 2869 2393 1611 950 674 266 253 219 187 110 BsrGI T'GTAC_A Cuts at: 96 3515 96 Size: 3419 6113 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9532 BssSI C'ACGA_G Cuts at: 7592 8604 7592 Size: 1012 8520 BstAPI GCAn_nnn'nTGC Cuts at: 794 3336 3486 4653 794 Size: 2542 150 1167 5673 Fragments arranged by size: 5673 2542 1167 150 BstBI TT'CG_AA Cuts at: 8336 8336 Size: 9532 BstEII G'GTnAC_C Cuts at: 1184 2248 2257 1184 Size: 1064 9 8459 Fragments arranged by size: 8459 1064 9 BstXI CCAn_nnnn'nTGG Cuts at: 4751 4751 Size: 9532 BstYI r'GATC_y Cuts at: 1972 4970 5737 5802 6370 6862 7229 7614 7631 Size: 2998 767 65 568 492 367 385 17 Cuts at: 7631 9083 9169 9181 9527 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2998 1977 1452 767 568 492 385 367 346 86 65 17 12 Bsu36I CC'TnA_GG Cuts at: 4965 4965 Size: 9532 BtgI C'CryG_G Cuts at: 513 1065 2088 2112 2575 2984 3091 513 Size: 552 1023 24 463 409 107 6954 Fragments arranged by size: 6954 1023 552 463 409 107 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8793 Fragments arranged by size: 8793 683 56 BtsI GCAGTG_nn' Cuts at: 4273 4501 4569 4721 4847 6250 7048 7842 7862 Size: 228 68 152 126 1403 798 794 20 Cuts at: 7862 4273 Size: 5943 Fragments arranged by size: 5943 1403 798 794 228 152 126 68 20 ClaI AT'CG_AT Cuts at: 1554 4506 1554 Size: 2952 6580 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2191 2226 3955 3990 4044 4079 4353 Size: 35 1627 35 1729 35 54 35 274 Cuts at: 4353 4388 529 Size: 35 5673 Fragments arranged by size: 5673 1729 1627 274 54 35 35 35 35 35 DraI TTT'AAA Cuts at: 6836 6836 Size: 9532 DraIII CAC_nnn'GTG Cuts at: 2355 3231 3233 2355 Size: 876 2 8654 Fragments arranged by size: 8654 876 2 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4158 8539 809 Size: 507 2842 4381 1802 Fragments arranged by size: 4381 2842 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2082 2141 Size: 54 1425 50 275 18 237 15 59 Cuts at: 2141 2196 2376 2981 3088 4741 4858 6150 6979 Size: 55 180 605 107 1653 117 1292 829 Cuts at: 6979 7868 9497 8 Size: 889 1629 43 Fragments arranged by size: 1653 1629 1425 1292 889 829 605 275 237 180 117 107 59 55 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2082 2141 2981 6150 6979 1537 Size: 275 270 59 840 3169 829 4090 Fragments arranged by size: 4090 3169 840 829 275 270 59 EarI CTCTTCn'nnn_ Cuts at: 1353 3196 4126 5373 5541 5688 7462 1353 Size: 1843 930 1247 168 147 1774 3423 Fragments arranged by size: 3423 1843 1774 1247 930 168 147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2208 3155 6269 6305 8119 8493 Size: 1064 138 619 947 3114 36 1814 374 Cuts at: 8493 8639 387 Size: 146 1280 Fragments arranged by size: 3114 1814 1280 1064 947 619 374 146 138 36 EcoICRI GAG'CTC Cuts at: 4420 4420 Size: 9532 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3339 3627 3862 4065 4353 4574 4956 Size: 393 1707 288 235 203 288 221 382 Cuts at: 4956 4967 5135 5870 5999 6072 6222 6707 6791 Size: 11 168 735 129 73 150 485 84 Cuts at: 6791 7595 8192 8979 9247 1239 Size: 804 597 787 268 1524 Fragments arranged by size: 1707 1524 804 787 735 597 485 393 382 288 288 268 235 221 203 168 150 129 84 73 11 EcoNI CCTnn'n_nnAGG Cuts at: 3631 4212 5264 3631 Size: 581 1052 7899 Fragments arranged by size: 7899 1052 581 EcoO109I rG'GnC_Cy Cuts at: 2514 5518 5929 6043 6235 2514 Size: 3004 411 114 192 5811 Fragments arranged by size: 5811 3004 411 192 114 EcoRI G'AATT_C Cuts at: 2627 6841 7219 2627 Size: 4214 378 4940 Fragments arranged by size: 4940 4214 378 EcoRV GAT'ATC Cuts at: 3643 6739 3643 Size: 3096 6436 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3892 3892 Size: 9532 FspI TGC'GCA Cuts at: 8038 8038 Size: 9532 HaeII r_GCGC'y Cuts at: 1678 2152 2697 3135 4906 6309 8679 1678 Size: 474 545 438 1771 1403 2370 2531 Fragments arranged by size: 2531 2370 1771 1403 545 474 438 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3709 3741 4189 4221 4404 Size: 32 135 32 2137 32 448 32 183 Cuts at: 4404 4436 8173 8205 8247 8279 1373 Size: 32 3737 32 42 32 2626 Fragments arranged by size: 3737 2626 2137 448 183 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2170 4915 5290 5628 6876 7122 Size: 988 281 232 2745 375 338 1248 246 Cuts at: 7122 669 Size: 3079 Fragments arranged by size: 3079 2745 1248 988 375 338 281 246 232 HindIII A'AGCT_T Cuts at: 748 2427 5558 748 Size: 1679 3131 4722 Fragments arranged by size: 4722 3131 1679 HpaI GTT'AAC Cuts at: 7122 7122 Size: 9532 KasI G'GCGC_C Cuts at: 3131 3131 Size: 9532 KpnI G_GTAC'C Cuts at: 6857 6857 Size: 9532 MfeI C'AATT_G Cuts at: 3421 4657 7131 3421 Size: 1236 2474 5822 Fragments arranged by size: 5822 2474 1236 MluI A'CGCG_T Cuts at: 9408 9408 Size: 9532 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4501 8646 8830 1305 Size: 3196 4145 184 2007 Fragments arranged by size: 4145 3196 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2198 3090 4743 10 Size: 54 1425 343 366 892 1653 4799 Fragments arranged by size: 4799 1653 1425 892 366 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2444 4984 5873 6464 7490 7849 8008 Size: 648 1278 2540 889 591 1026 359 159 Cuts at: 8008 518 Size: 2042 Fragments arranged by size: 2540 2042 1278 1026 889 648 591 359 159 MspA1I CmG'CkG Cuts at: 1606 2090 2577 3255 4695 5824 6574 6583 6948 Size: 484 487 678 1440 1129 750 9 365 Cuts at: 6948 7625 8773 9018 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1440 1148 1129 750 678 677 487 484 365 245 9 NaeI GCC'GGC Cuts at: 2031 2031 Size: 9532 NarI GG'CG_CC Cuts at: 3132 3132 Size: 9532 NcoI C'CATG_G Cuts at: 513 1065 2112 3091 513 Size: 552 1047 979 6954 Fragments arranged by size: 6954 1047 979 552 NdeI CA'TA_TG Cuts at: 387 2938 387 Size: 2551 6981 NgoMIV G'CCGG_C Cuts at: 2029 2029 Size: 9532 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9532 NotI GC'GGCC_GC Cuts at: 2082 2141 6979 2082 Size: 59 4838 4635 Fragments arranged by size: 4838 4635 59 NspI r_CATG'y Cuts at: 1132 1229 2158 6890 8435 1132 Size: 97 929 4732 1545 2229 Fragments arranged by size: 4732 2229 1545 929 97 PasI CC'CwG_GG Cuts at: 1348 4450 6238 1348 Size: 3102 1788 4642 Fragments arranged by size: 4642 3102 1788 PciI A'CATG_T Cuts at: 8431 8431 Size: 9532 PflMI CCAn_nnn'nTGG Cuts at: 1108 2355 4822 1108 Size: 1247 2467 5818 Fragments arranged by size: 5818 2467 1247 PfoI T'CCnGG_A Cuts at: 4330 5151 4330 Size: 821 8711 PmeI GTTT'AAAC Cuts at: 6836 6836 Size: 9532 PmlI CAC'GTG Cuts at: 2208 4735 2208 Size: 2527 7005 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2546 2578 7601 7633 9114 9146 2546 Size: 32 5023 32 1481 32 2932 Fragments arranged by size: 5023 2932 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5518 5929 5518 Size: 411 9121 PshAI GACnn'nnGTC Cuts at: 1613 2255 1613 Size: 642 8890 PsiI TTA'TAA Cuts at: 7102 7102 Size: 9532 PspOMI G'GGCC_C Cuts at: 2513 2513 Size: 9532 PstI C_TGCA'G Cuts at: 830 2304 2727 4232 4297 4785 6411 6884 830 Size: 1474 423 1505 65 488 1626 473 3478 Fragments arranged by size: 3478 1626 1505 1474 488 473 423 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9532 PvuII CAG'CTG Cuts at: 3255 4695 5824 6574 6583 3255 Size: 1440 1129 750 9 6204 Fragments arranged by size: 6204 1440 1129 750 9 SacI G_AGCT'C Cuts at: 4422 4422 Size: 9532 SacII CC_GC'GG Cuts at: 2091 2578 2091 Size: 487 9045 SalI G'TCGA_C Cuts at: 1936 6874 1936 Size: 4938 4594 SanDI GG'GwC_CC Cuts at: 5518 5518 Size: 9532 SapI GCTCTTCn'nnn_ Cuts at: 3196 5688 3196 Size: 2492 7040 SbfI CC_TGCA'GG Cuts at: 6884 6884 Size: 9532 ScaI AGT'ACT Cuts at: 2534 7780 9492 2534 Size: 5246 1712 2574 Fragments arranged by size: 5246 2574 1712 SexAI A'CCwGG_T Cuts at: 2351 2351 Size: 9532 SfcI C'TryA_G Cuts at: 826 1045 2300 2536 2723 2847 3202 3532 4228 Size: 219 1255 236 187 124 355 330 696 Cuts at: 4228 4293 4781 5312 5511 6407 6880 8015 8696 Size: 65 488 531 199 896 473 1135 681 Cuts at: 8696 8887 826 Size: 191 1471 Fragments arranged by size: 1471 1255 1135 896 696 681 531 488 473 355 330 236 219 199 191 187 124 65 SfiI GGCCn_nnn'nGGCC Cuts at: 2030 3096 5780 2030 Size: 1066 2684 5782 Fragments arranged by size: 5782 2684 1066 SfoI GGC'GCC Cuts at: 3133 3133 Size: 9532 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9532 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9532 SmaI CCC'GGG Cuts at: 1872 2045 6859 1872 Size: 173 4814 4545 Fragments arranged by size: 4814 4545 173 SmlI C'TyrA_G Cuts at: 820 1017 1943 2453 2813 3617 3794 3808 5459 Size: 197 926 510 360 804 177 14 1651 Cuts at: 5459 6492 7636 8537 8799 820 Size: 1033 1144 901 262 1553 Fragments arranged by size: 1651 1553 1144 1033 926 901 804 510 360 262 197 177 14 SnaBI TAC'GTA Cuts at: 493 7323 493 Size: 6830 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9532 SphI G_CATG'C Cuts at: 1132 2158 6890 1132 Size: 1026 4732 3774 Fragments arranged by size: 4732 3774 1026 SspI AAT'ATT Cuts at: 5 52 7456 5 Size: 47 7404 2081 Fragments arranged by size: 7404 2081 47 StuI AGG'CCT Cuts at: 1517 2612 3913 1517 Size: 1095 1301 7136 Fragments arranged by size: 7136 1301 1095 StyI C'CwwG_G Cuts at: 513 1065 2112 2565 3091 3169 3905 4821 5179 Size: 552 1047 453 526 78 736 916 358 Cuts at: 5179 6039 6333 6971 513 Size: 860 294 638 3074 Fragments arranged by size: 3074 1047 916 860 736 638 552 526 453 358 294 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2339 3567 4051 5061 7559 7576 7729 Size: 1255 759 1228 484 1010 2498 17 153 Cuts at: 7729 7914 8359 325 Size: 185 445 1498 Fragments arranged by size: 2498 1498 1255 1228 1010 759 484 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2532 2618 3515 Size: 274 80 33 51 1443 555 86 897 Cuts at: 3515 5761 7778 9490 96 Size: 2246 2017 1712 138 Fragments arranged by size: 2246 2017 1712 1443 897 555 274 138 86 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2775 2807 3970 4002 4361 4372 4393 4404 9492 Size: 32 1163 32 359 11 21 11 5088 Cuts at: 9492 9524 2775 Size: 32 2783 Fragments arranged by size: 5088 2783 1163 359 32 32 32 21 11 11 Tth111I GACn'n_nGTC Cuts at: 1316 3407 1316 Size: 2091 7441 XbaI T'CTAG_A Cuts at: 6868 6868 Size: 9532 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2537 4925 2537 Size: 2388 7144 XhoI C'TCGA_G Cuts at: 1943 3617 1943 Size: 1674 7858 XmaI C'CCGG_G Cuts at: 1870 2043 6857 1870 Size: 173 4814 4545 Fragments arranged by size: 4814 4545 173 XmnI GAAnn'nnTTC Cuts at: 3161 7661 3161 Size: 4500 5032 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7340 276 Size: 53 83 186 936 5806 2468 Fragments arranged by size: 5806 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SrfI SwaI