(Circular) (Six-base) MAPSORT of: pFN21AB5414.seq Check: 9234 from: 1 to: 5728 pFN21AB5414 5728 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2500 2500 Size: 5728 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3538 278 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3071 1937 Size: 1134 4594 Acc65I G'GTAC_C Cuts at: 2594 3049 2594 Size: 455 5273 AclI AA'CG_TT Cuts at: 1563 3855 4228 1563 Size: 2292 373 3063 Fragments arranged by size: 3063 2292 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2469 2493 2862 3791 5175 5443 1632 Size: 837 24 369 929 1384 268 1917 Fragments arranged by size: 1917 1384 929 837 369 268 24 AflII C'TTAA_G Cuts at: 820 1017 2999 820 Size: 197 1982 3549 Fragments arranged by size: 3549 1982 197 AflIII A'CryG_T Cuts at: 1203 4627 5604 1203 Size: 3424 977 1327 Fragments arranged by size: 3424 1327 977 AgeI A'CCGG_T Cuts at: 4559 4559 Size: 5728 AhdI GACnn_n'nnGTC Cuts at: 4457 4457 Size: 5728 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5728 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5728 AlwNI CAG_nnn'CTG Cuts at: 2063 2492 2614 3471 5043 2063 Size: 429 122 857 1572 2748 Fragments arranged by size: 2748 1572 857 429 122 ApaLI G'TGCA_C Cuts at: 3785 4941 3785 Size: 1156 4572 ApoI r'AATT_y Cuts at: 1493 2382 2412 3037 3263 3415 5524 1493 Size: 889 30 625 226 152 2109 1697 Fragments arranged by size: 2109 1697 889 625 226 152 30 AseI AT'TA_AT Cuts at: 160 1032 4282 160 Size: 872 3250 1606 Fragments arranged by size: 3250 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 AvaI C'yCGr_G Cuts at: 1870 1943 2895 3053 1870 Size: 73 952 158 4545 Fragments arranged by size: 4545 952 158 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2578 2611 2578 Size: 33 5695 BamHI G'GATC_C Cuts at: 3058 3425 3058 Size: 367 5361 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2512 2594 3049 4504 618 Size: 325 217 627 725 82 455 1455 1842 Fragments arranged by size: 1842 1455 725 627 455 325 217 82 BanII G_rGCy'C Cuts at: 1391 3047 1391 Size: 1656 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2259 928 Size: 971 360 4397 Fragments arranged by size: 4397 971 360 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3919 3953 1903 Size: 34 1982 34 3678 Fragments arranged by size: 3678 1982 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3620 4830 3620 Size: 1210 4518 BclI T'GATC_A Cuts at: 1548 1548 Size: 5728 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2185 4339 136 Size: 107 122 71 631 605 513 2154 1525 Fragments arranged by size: 2154 1525 631 605 513 122 107 71 BglII A'GATC_T Cuts at: 2700 5723 2700 Size: 3023 2705 BlpI GC'TnA_GC Cuts at: 3145 3145 Size: 5728 Bme1580I G_kGCm'C Cuts at: 1790 2712 3789 4945 1790 Size: 922 1077 1156 2573 Fragments arranged by size: 2573 1156 1077 922 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5728 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3459 4417 442 Size: 416 53 83 399 24 2042 958 1753 Fragments arranged by size: 2042 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5728 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2040 2763 2817 4388 1239 Size: 801 723 54 1571 2579 Fragments arranged by size: 2579 1571 801 723 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3853 4718 5016 3853 Size: 865 298 4565 Fragments arranged by size: 4565 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4391 882 Size: 617 2892 2219 Fragments arranged by size: 2892 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3519 493 Size: 711 2315 2702 Fragments arranged by size: 2702 2315 711 BsaBI GATnn'nnATC Cuts at: 5722 5722 Size: 5728 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3535 3917 4552 275 Size: 53 83 186 936 2002 382 635 1451 Fragments arranged by size: 2002 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2297 4161 4559 4833 4980 5391 2297 Size: 1864 398 274 147 411 2634 Fragments arranged by size: 2634 1864 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5503 Fragments arranged by size: 5503 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2492 2583 2999 1186 Size: 1306 91 416 3915 Fragments arranged by size: 3915 1306 416 91 BseYI C'CCAG_C Cuts at: 2091 2223 2711 2818 4931 5427 2091 Size: 132 488 107 2113 496 2392 Fragments arranged by size: 2392 2113 496 488 132 107 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5155 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3178 3939 4967 1540 Size: 275 186 1177 761 1028 2301 Fragments arranged by size: 2301 1177 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3789 3874 4945 3789 Size: 85 1071 4572 Fragments arranged by size: 4572 1071 85 BsmI GAATG_Cn' Cuts at: 3239 3332 3239 Size: 93 5635 BsmBI CGTCTCn'nnnn_ Cuts at: 2739 2739 Size: 5728 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2712 3047 3789 3874 4945 1391 Size: 399 922 335 742 85 1071 2174 Fragments arranged by size: 2174 1071 922 742 399 335 85 BspEI T'CCGG_A Cuts at: 2297 5391 2297 Size: 3094 2634 BspHI T'CATG_A Cuts at: 3617 5347 3617 Size: 1730 3998 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2500 3083 844 Size: 1656 583 3489 Fragments arranged by size: 3489 1656 583 BsrBI CCG'CTC Cuts at: 1394 1823 3615 1394 Size: 429 1792 3507 Fragments arranged by size: 3507 1792 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4223 4397 65 Size: 1183 2975 174 1396 Fragments arranged by size: 2975 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4372 4559 1700 Size: 110 2562 187 2869 Fragments arranged by size: 2869 2562 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5728 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5728 BssSI C'ACGA_G Cuts at: 3788 4800 3788 Size: 1012 4716 BstAPI GCAn_nnn'nTGC Cuts at: 794 2138 794 Size: 1344 4384 BstBI TT'CG_AA Cuts at: 4532 4532 Size: 5728 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5728 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2039 2011 Size: 28 5700 BstYI r'GATC_y Cuts at: 1972 2700 3058 3425 3810 3827 5279 5365 5377 Size: 728 358 367 385 17 1452 86 12 Cuts at: 5377 5723 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 728 385 367 358 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2010 2203 513 Size: 552 945 193 4038 Fragments arranged by size: 4038 945 552 193 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4989 Fragments arranged by size: 4989 683 56 BtsI GCAGTG_nn' Cuts at: 2545 2958 3244 4038 4058 2545 Size: 413 286 794 20 4215 Fragments arranged by size: 4215 794 413 286 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5728 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5693 DraI TTT'AAA Cuts at: 3032 3032 Size: 5728 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4735 809 Size: 507 3419 1802 Fragments arranged by size: 3419 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3175 4064 5693 Size: 54 1425 50 275 18 1345 889 1629 Cuts at: 5693 8 Size: 43 Fragments arranged by size: 1629 1425 1345 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3175 1537 Size: 275 1363 4090 Fragments arranged by size: 4090 1363 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2457 2469 3658 1353 Size: 1104 12 1189 3423 Fragments arranged by size: 3423 1189 1104 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4315 4689 4835 387 Size: 1064 138 2726 374 146 1280 Fragments arranged by size: 2726 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2040 2469 2493 2763 2817 2862 3791 Size: 393 408 429 24 270 54 45 929 Cuts at: 3791 4388 5175 5443 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 929 787 597 429 408 393 270 268 54 45 24 EcoO109I rG'GnC_Cy Cuts at: 2611 3004 2611 Size: 393 5335 EcoRI G'AATT_C Cuts at: 3037 3415 3037 Size: 378 5350 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2415 2415 Size: 5728 FspI TGC'GCA Cuts at: 4234 4234 Size: 5728 HaeII r_GCGC'y Cuts at: 1678 4875 1678 Size: 3197 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2685 2717 4369 4401 4443 Size: 32 135 32 1113 32 1652 32 42 Cuts at: 4443 4475 1373 Size: 32 2626 Fragments arranged by size: 2626 1652 1113 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3072 3318 669 Size: 988 281 1134 246 3079 Fragments arranged by size: 3079 1134 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5728 HpaI GTT'AAC Cuts at: 3318 3318 Size: 5728 KpnI G_GTAC'C Cuts at: 2598 3053 2598 Size: 455 5273 MfeI C'AATT_G Cuts at: 3327 3327 Size: 5728 MluI A'CGCG_T Cuts at: 5604 5604 Size: 5728 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4842 5026 1305 Size: 3537 184 2007 Fragments arranged by size: 3537 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3906 Fragments arranged by size: 3906 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3686 4045 4204 518 Size: 648 843 1677 359 159 2042 Fragments arranged by size: 2042 1677 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2051 2176 2581 3144 3821 4969 5214 1606 Size: 445 125 405 563 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 563 445 405 245 125 NcoI C'CATG_G Cuts at: 513 1065 2010 2203 513 Size: 552 945 193 4038 Fragments arranged by size: 4038 945 552 193 NdeI CA'TA_TG Cuts at: 387 387 Size: 5728 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5728 NotI GC'GGCC_GC Cuts at: 3175 3175 Size: 5728 NspI r_CATG'y Cuts at: 1132 1229 3086 4631 1132 Size: 97 1857 1545 2229 Fragments arranged by size: 2229 1857 1545 97 PasI CC'CwG_GG Cuts at: 1348 2563 1348 Size: 1215 4513 PciI A'CATG_T Cuts at: 4627 4627 Size: 5728 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5728 PmeI GTTT'AAAC Cuts at: 3032 3032 Size: 5728 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3797 3829 5310 5342 3797 Size: 32 1481 32 4183 Fragments arranged by size: 4183 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3004 3004 Size: 5728 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5728 PsiI TTA'TAA Cuts at: 3298 3298 Size: 5728 PstI C_TGCA'G Cuts at: 830 3080 830 Size: 2250 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5728 PvuII CAG'CTG Cuts at: 2051 2176 2581 2051 Size: 125 405 5198 Fragments arranged by size: 5198 405 125 SalI G'TCGA_C Cuts at: 1936 3070 1936 Size: 1134 4594 SbfI CC_TGCA'GG Cuts at: 3080 3080 Size: 5728 ScaI AGT'ACT Cuts at: 3976 5688 3976 Size: 1712 4016 SfcI C'TryA_G Cuts at: 826 1045 2404 2669 3076 4211 4892 5083 826 Size: 219 1359 265 407 1135 681 191 1471 Fragments arranged by size: 1471 1359 1135 681 407 265 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5728 SmaI CCC'GGG Cuts at: 1872 3055 1872 Size: 1183 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2895 2999 3832 4733 4995 820 Size: 197 926 952 104 833 901 262 1553 Fragments arranged by size: 1553 952 926 901 833 262 197 104 SnaBI TAC'GTA Cuts at: 493 3519 493 Size: 3026 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5728 SphI G_CATG'C Cuts at: 1132 3086 1132 Size: 1954 3774 SspI AAT'ATT Cuts at: 5 52 3652 5 Size: 47 3600 2081 Fragments arranged by size: 3600 2081 47 StuI AGG'CCT Cuts at: 1517 2398 1517 Size: 881 4847 StyI C'CwwG_G Cuts at: 513 1065 2010 2203 2450 3167 513 Size: 552 945 193 247 717 3074 Fragments arranged by size: 3074 945 717 552 247 193 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2791 3755 3772 3925 4110 4555 325 Size: 1255 1211 964 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1211 964 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2170 3974 5686 Size: 274 80 33 51 1443 193 1804 1712 Cuts at: 5686 96 Size: 138 Fragments arranged by size: 1804 1712 1443 274 193 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5688 5720 5688 Size: 32 5696 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5728 XbaI T'CTAG_A Cuts at: 3064 3064 Size: 5728 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2537 2537 Size: 5728 XhoI C'TCGA_G Cuts at: 1943 2895 1943 Size: 952 4776 XmaI C'CCGG_G Cuts at: 1870 3053 1870 Size: 1183 4545 XmnI GAAnn'nnTTC Cuts at: 3857 3857 Size: 5728 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3536 276 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI