Restriction map for
pFN21AB1488
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# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AscI AvrII BciVI BfrI BlnI
BsaBI BseAI BsiWI BsmBI BspDI
BspEI BspLU11I BssHII Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraI
EcoO65I EcoRV EcoT22I FseI HincII
HindII HindIII HpaI KspI MamI
MfeI MluI MroI MunI NheI
NotI NruI NsiI PacI PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI ScaI SexAI SgrAI SnaBI
SpeI SwaI XbaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 4459 GACGTC GACGT^C
Acc65I 470 GGTACC G^GTACC
AcsI 2758 GAATTC [GA]^AATT[CT]
AhdI 355 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4396 GTGCAC G^TGCAC
AocI 316 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2099 AGCGCT AGC^GCT
ApaLI 4396 GTGCAC G^TGCAC
ApoI 2758 GAATTC [GA]^AATT[CT]
AseI 2059 ATTAAT AT^TAAT
AsnI 2059 ATTAAT AT^TAAT
Asp718I 470 GGTACC G^GTACC
AspEI 355 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4579 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3164 GGATCC G^GATCC
BbvCI 4771 GCTGAGG CCTCAGC(-5/-2)
BclI 5051 TGATCA T^GATCA
BglII 641 AGATCT A^GATCT
BlpI 1716 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1716 GCTCAGC GC^T[ACGT]AGC
BsaWI 1742 ACCGGA [AT]^CCGG[AT]
BsiEI 1429 CGGCCG CG[GA][CT]^CG
Bsp1407I 572 TGTACA T^GTACA
BspHI 1550 TCATGA T^CATGA
BsrGI 572 TGTACA T^GTACA
BstBI 2755 TTCGAA TT^CGAA
Bsu36I 316 CCTGAGG CC^T[ACGT]AGG
CelII 1716 GCTCAGC GC^T[ACGT]AGC
DrdI 1997 GACTAAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1429 CGGCCG C^GGCCG
Eam1105I 355 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1429 CGGCCG C^GGCCG
Eco47III 2099 AGCGCT AGC^GCT
Eco52I 1429 CGGCCG C^GGCCG
Eco81I 316 CCTGAGG CC^T[ACGT]AGG
EcoRI 2758 GAATTC G^AATTC
FbaI 5051 TGATCA T^GATCA
KpnI 470 GGTACC GGTAC^C
NaeI 4650 GCCGGC GCC^GGC
NdeI 839 CATATG CA^TATG
NgoMIV 4650 GCCGGC G^CCGGC
NspV 2755 TTCGAA TT^CGAA
PflFI 4579 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2059 ATTAAT AT^TAAT
RcaI 1550 TCATGA T^CATGA
SbfI 2280 CCTGCAGG CCTGCA^GG
SfiI 2248 GGCCAGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2755 TTCGAA TT^CGAA
SphI 3380 GCATGC GCATG^C
Sse8387I 2280 CCTGCAGG CCTGCA^GG
SspBI 572 TGTACA T^GTACA
Tth111I 4579 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AflIII 2122 ACACGT A^C[GA][CT]GT
2603 ACGTGT
ApaI 1142 GGGCCC GGGCC^C
4555 GGGCCC
Asp700I 876 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4008 GAATGGGTTC
BbeI 3612 GGCGCC GGCGC^C
4362 GGCGCC
BbrPI 2412 CACGTG CAC^GTG
2602 CACGTG
BcgI 5008 CGAGCCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4445 GCATGGCCATCG
BfaI 859 CTAG C^TAG
1786 CTAG
BsmI 1199 GCATTC GAATGC(1/-1)
1295 GCATTC
BsrFI 4632 GCCGGT [GA]^CCGG[CT]
4650 GCCGGC
BssSI 3823 CACGAG CACGAG(-5/-1)
1448 CTCGTG
Cfr10I 4632 GCCGGT [GA]^CCGG[CT]
4650 GCCGGC
KasI 3612 GGCGCC G^GCGCC
4362 GGCGCC
MaeI 859 CTAG C^TAG
1786 CTAG
NarI 3612 GGCGCC GG^CGCC
4362 GGCGCC
PmaCI 2412 CACGTG CAC^GTG
2602 CACGTG
PmlI 2412 CACGTG CAC^GTG
2602 CACGTG
PshAI 1578 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3355 GACCCCTGTC
SacI 1066 GAGCTC GAGCT^C
1714 GAGCTC
SfoI 3612 GGCGCC GGC^GCC
4362 GGCGCC
SmaI 1408 CCCGGG CCC^GGG
4595 CCCGGG
SspI 2379 AATATT AAT^ATT
5045 AATATT
StuI 1160 AGGCCT AGG^CCT
2451 AGGCCT
XmaCI 1408 CCCGGG C^CCGGG
4595 CCCGGG
XmaI 1408 CCCGGG C^CCGGG
4595 CCCGGG
XmnI 876 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4008 GAATGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
AviII BalI BanI BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PaeR7I PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoI XhoII