(Circular) (Six-base) MAPSORT of: pFN21AB1488.seq Check: 4456 from: 1 to: 9937 pFN21AB1488 9937 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4515 4651 4515 Size: 136 9801 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6468 7747 278 Size: 53 83 186 936 4932 1279 2468 Fragments arranged by size: 4932 2468 1279 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7280 1937 Size: 5343 4594 Acc65I G'GTAC_C Cuts at: 2475 7258 2475 Size: 4783 5154 AclI AA'CG_TT Cuts at: 1563 8064 8437 1563 Size: 6501 373 3063 Fragments arranged by size: 6501 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3273 3387 3920 4757 6158 6780 8000 9384 Size: 1641 114 533 837 1401 622 1220 1384 Cuts at: 9384 9652 1632 Size: 268 1917 Fragments arranged by size: 1917 1641 1401 1384 1220 837 622 533 268 114 AfeI AGC'GCT Cuts at: 4106 4106 Size: 9937 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9740 AflIII A'CryG_T Cuts at: 1203 4127 4608 8836 9813 1203 Size: 2924 481 4228 977 1327 Fragments arranged by size: 4228 2924 1327 977 481 AgeI A'CCGG_T Cuts at: 8768 8768 Size: 9937 AhdI GACnn_n'nnGTC Cuts at: 2365 8666 2365 Size: 6301 3636 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9937 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9937 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3743 3775 3743 Size: 32 9905 AlwNI CAG_nnn'CTG Cuts at: 3010 3200 3350 4666 5232 5705 7108 7680 9252 Size: 190 150 1316 566 473 1403 572 1572 Cuts at: 9252 3010 Size: 3695 Fragments arranged by size: 3695 1572 1403 1316 572 566 473 190 150 ApaI G_GGCC'C Cuts at: 3151 6564 3151 Size: 3413 6524 ApaLI G'TGCA_C Cuts at: 6401 7994 9150 6401 Size: 1593 1156 7188 Fragments arranged by size: 7188 1593 1156 ApoI r'AATT_y Cuts at: 1493 4763 7246 7472 7624 9733 1493 Size: 3270 2483 226 152 2109 1697 Fragments arranged by size: 3270 2483 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4065 8491 160 Size: 872 3033 4426 1606 Fragments arranged by size: 4426 3033 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9937 AvaI C'yCGr_G Cuts at: 1870 1943 2145 2509 3413 3537 4749 5025 5120 Size: 73 202 364 904 124 1212 276 95 Cuts at: 5120 6600 6689 7088 7262 1870 Size: 1480 89 399 174 4545 Fragments arranged by size: 4545 1480 1212 904 399 364 276 202 174 124 95 89 73 BamHI G'GATC_C Cuts at: 5169 7267 7634 5169 Size: 2098 367 7472 Fragments arranged by size: 7472 2098 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2269 2421 2475 2767 3616 Size: 325 217 627 482 152 54 292 849 Cuts at: 3616 3734 4669 4708 5570 5617 6367 6718 7227 Size: 118 935 39 862 47 750 351 509 Cuts at: 7227 7258 8713 618 Size: 31 1455 1842 Fragments arranged by size: 1842 1455 935 862 849 750 627 509 482 351 325 292 217 152 118 54 47 39 31 BanII G_rGCy'C Cuts at: 1391 2862 3075 3151 3570 3723 4197 4276 4682 Size: 1471 213 76 419 153 474 79 406 Cuts at: 4682 5565 5699 6564 7256 1391 Size: 883 134 865 692 4072 Fragments arranged by size: 4072 1471 883 865 692 474 419 406 213 153 134 79 76 BbeI G_GCGC'C Cuts at: 5621 6371 5621 Size: 750 9187 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2888 3225 4028 5461 6912 928 Size: 971 989 337 803 1433 1451 3953 Fragments arranged by size: 3953 1451 1433 989 971 803 337 BbvCI CC'TCA_GC Cuts at: 6777 6777 Size: 9937 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6439 6473 7002 7036 8128 8162 1903 Size: 34 4502 34 529 34 1092 34 3678 Fragments arranged by size: 4502 3678 1092 529 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7829 9039 7829 Size: 1210 8727 BclI T'GATC_A Cuts at: 1548 7056 1548 Size: 5508 4429 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3800 4260 5591 Size: 107 122 71 631 605 2128 460 1331 Cuts at: 5591 6643 8548 136 Size: 1052 1905 1525 Fragments arranged by size: 2128 1905 1525 1331 1052 631 605 460 122 107 71 BglII A'GATC_T Cuts at: 2646 9932 2646 Size: 7286 2651 BlpI GC'TnA_GC Cuts at: 3722 7354 3722 Size: 3632 6305 Bme1580I G_kGCm'C Cuts at: 1790 2145 2274 2424 3151 3934 4713 5575 6405 Size: 355 129 150 727 783 779 862 830 Cuts at: 6405 6564 6721 6791 7189 7998 9154 1790 Size: 159 157 70 398 809 1156 2573 Fragments arranged by size: 2573 1156 862 830 809 783 779 727 398 355 159 157 150 129 70 BmgBI CAC'GTC Cuts at: 1318 4130 6223 6568 1318 Size: 2812 2093 345 4687 Fragments arranged by size: 4687 2812 2093 345 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2686 3656 4469 Size: 416 53 83 399 24 1269 970 813 Cuts at: 4469 4610 5701 7096 7668 8626 442 Size: 141 1091 1395 572 958 1753 Fragments arranged by size: 1753 1395 1269 1091 970 958 813 572 416 399 141 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9937 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5040 7159 5040 Size: 2119 7818 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2249 2921 2987 2993 3030 3737 4298 Size: 878 132 672 66 6 37 707 561 Cuts at: 4298 4375 4571 5697 6086 6527 6830 7190 8597 Size: 77 196 1126 389 441 303 360 1407 Cuts at: 8597 1239 Size: 2579 Fragments arranged by size: 2579 1407 1126 878 707 672 561 441 389 360 303 196 132 77 66 37 6 Bpu10I CC'TnA_GC Cuts at: 2231 4436 6777 2231 Size: 2205 2341 5391 Fragments arranged by size: 5391 2341 2205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5035 8062 8927 9225 5035 Size: 3027 865 298 5747 Fragments arranged by size: 5747 3027 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3595 3648 3969 5117 5987 6545 6929 Size: 617 2096 53 321 1148 870 558 384 Cuts at: 6929 8600 882 Size: 1671 2219 Fragments arranged by size: 2219 2096 1671 1148 870 617 558 384 321 53 BsaAI yAC'GTr Cuts at: 493 1204 4419 4609 6538 7728 493 Size: 711 3215 190 1929 1190 2702 Fragments arranged by size: 3215 2702 1929 1190 711 190 BsaBI GATnn'nnATC Cuts at: 9931 9931 Size: 9937 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2967 5618 6368 6465 Size: 53 83 186 936 1434 2651 750 97 Cuts at: 6465 7744 8126 8761 275 Size: 1279 382 635 1451 Fragments arranged by size: 2651 1451 1434 1279 936 750 635 382 186 97 83 53 BsaWI w'CCGG_w Cuts at: 3747 8370 8768 9042 9189 9600 3747 Size: 4623 398 274 147 411 4084 Fragments arranged by size: 4623 4084 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4383 4413 4613 4643 4767 Size: 30 165 30 2980 30 200 30 124 Cuts at: 4767 4797 5101 5131 5725 5755 6065 6095 6377 Size: 30 304 30 594 30 310 30 282 Cuts at: 6377 6407 1178 Size: 30 4708 Fragments arranged by size: 4708 2980 594 310 304 282 200 165 124 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2246 2300 2398 3077 3441 4307 4844 5733 Size: 1060 54 98 679 364 866 537 889 Cuts at: 5733 5987 6083 6086 6398 7148 1186 Size: 254 96 3 312 750 3975 Fragments arranged by size: 3975 1060 889 866 750 679 537 364 312 254 98 96 54 3 BseYI C'CCAG_C Cuts at: 3193 3695 3805 4637 4656 5331 5558 5776 7188 Size: 502 110 832 19 675 227 218 1412 Cuts at: 7188 9140 9636 3193 Size: 1952 496 3494 Fragments arranged by size: 3494 1952 1412 832 675 502 496 227 218 110 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2147 2852 4193 4493 5505 5798 6647 Size: 573 411 705 1341 300 1012 293 849 Cuts at: 6647 6671 6941 1163 Size: 24 270 4159 Fragments arranged by size: 4159 1341 1012 849 705 573 411 300 293 270 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3437 7387 8148 9176 1540 Size: 275 186 1436 3950 761 1028 2301 Fragments arranged by size: 3950 2301 1436 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2516 3075 3723 5111 6405 7166 7998 8083 9154 Size: 559 648 1388 1294 761 832 85 1071 Cuts at: 9154 2516 Size: 3299 Fragments arranged by size: 3299 1388 1294 1071 832 761 648 559 85 BsmI GAATG_Cn' Cuts at: 3204 3300 7448 7541 3204 Size: 96 4148 93 5600 Fragments arranged by size: 5600 4148 96 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2274 2424 2516 2862 3075 3151 Size: 399 355 129 150 92 346 213 76 Cuts at: 3151 3570 3723 3934 4197 4276 4682 4713 5111 Size: 419 153 211 263 79 406 31 398 Cuts at: 5111 5565 5575 5699 6405 6564 6721 6791 7166 Size: 454 10 124 706 159 157 70 375 Cuts at: 7166 7189 7256 7998 8083 9154 1391 Size: 23 67 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 706 454 419 406 399 398 375 355 346 263 213 211 159 157 153 150 129 124 92 85 79 76 70 67 31 23 10 BspEI T'CCGG_A Cuts at: 9600 9600 Size: 9937 BspHI T'CATG_A Cuts at: 3555 7826 9556 3555 Size: 4271 1730 3936 Fragments arranged by size: 4271 3936 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2406 4515 4651 5269 5637 6191 7116 7292 Size: 1562 2109 136 618 368 554 925 176 Cuts at: 7292 844 Size: 3489 Fragments arranged by size: 3489 2109 1562 925 618 554 368 176 136 BsrBI CCG'CTC Cuts at: 1394 1823 3288 3433 6814 7824 1394 Size: 429 1465 145 3381 1010 3507 Fragments arranged by size: 3507 3381 1465 1010 429 145 BsrDI GCAATG_nn' Cuts at: 65 1248 5413 5667 5725 8432 8606 65 Size: 1183 4165 254 58 2707 174 1396 Fragments arranged by size: 4165 2707 1396 1183 254 174 58 BsrFI r'CCGG_y Cuts at: 1700 1810 6637 6655 8581 8768 1700 Size: 110 4827 18 1926 187 2869 Fragments arranged by size: 4827 2869 1926 187 110 18 BsrGI T'GTAC_A Cuts at: 96 2577 96 Size: 2481 7456 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9937 BssSI C'ACGA_G Cuts at: 3453 5828 7997 9009 3453 Size: 2375 2169 1012 4381 Fragments arranged by size: 4381 2375 2169 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5821 6634 7184 794 Size: 5027 813 550 3547 Fragments arranged by size: 5027 3547 813 550 BstBI TT'CG_AA Cuts at: 4761 8741 4761 Size: 3980 5957 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9937 BstXI CCAn_nnnn'nTGG Cuts at: 2640 4347 5675 5784 6379 7214 2640 Size: 1707 1328 109 595 835 5363 Fragments arranged by size: 5363 1707 1328 835 595 109 BstYI r'GATC_y Cuts at: 1972 2646 3112 4281 4425 4954 5143 5169 7267 Size: 674 466 1169 144 529 189 26 2098 Cuts at: 7267 7634 8019 8036 9488 9574 9586 9932 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2098 1977 1452 1169 674 529 466 385 367 346 189 144 86 26 17 12 Bsu36I CC'TnA_GG Cuts at: 2322 2322 Size: 9937 BtgI C'CryG_G Cuts at: 513 1065 2348 3225 3489 3649 4429 4713 5139 Size: 552 1283 877 264 160 780 284 426 Cuts at: 5139 513 Size: 5311 Fragments arranged by size: 5311 1283 877 780 552 426 284 264 160 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5428 6442 487 Size: 683 56 4202 1014 3982 Fragments arranged by size: 4202 3982 1014 683 56 BtsI GCAGTG_nn' Cuts at: 3502 5108 5111 7453 8247 8267 3502 Size: 1606 3 2342 794 20 5172 Fragments arranged by size: 5172 2342 1606 794 20 3 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9937 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9902 DraI TTT'AAA Cuts at: 7241 7241 Size: 9937 DraIII CAC_nnn'GTG Cuts at: 3139 3664 5140 6414 6750 3139 Size: 525 1476 1274 336 6326 Fragments arranged by size: 6326 1476 1274 525 336 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4008 8944 809 Size: 507 2692 4936 1802 Fragments arranged by size: 4936 2692 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2461 3289 3434 Size: 54 1425 50 275 18 631 828 145 Cuts at: 3434 3666 4467 5463 6453 6657 6741 7384 8273 Size: 232 801 996 990 204 84 643 889 Cuts at: 8273 9902 8 Size: 1629 43 Fragments arranged by size: 1629 1425 996 990 889 828 801 643 631 275 232 204 145 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3434 7384 1537 Size: 275 1622 3950 4090 Fragments arranged by size: 4090 3950 1622 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2282 2408 4232 7867 1353 Size: 929 126 1824 3635 3423 Fragments arranged by size: 3635 3423 1824 929 126 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4505 4523 4659 5204 6439 6914 Size: 1064 138 2916 18 136 545 1235 475 Cuts at: 6914 8524 8898 9044 387 Size: 1610 374 146 1280 Fragments arranged by size: 2916 1610 1280 1235 1064 545 475 374 146 138 136 18 EcoICRI GAG'CTC Cuts at: 3073 3721 3073 Size: 648 9289 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2249 2921 2987 2993 3030 3273 Size: 393 485 132 672 66 6 37 243 Cuts at: 3273 3387 3737 3920 4298 4375 4571 4757 5697 Size: 114 350 183 378 77 196 186 940 Cuts at: 5697 6086 6158 6527 6780 6830 7190 8000 8597 Size: 389 72 369 253 50 360 810 597 Cuts at: 8597 9384 9652 1239 Size: 787 268 1524 Fragments arranged by size: 1524 940 810 787 672 597 485 393 389 378 369 360 350 268 253 243 196 186 183 132 114 77 72 66 50 37 6 EcoNI CCTnn'n_nnAGG Cuts at: 2867 2948 3161 3405 4289 6068 2867 Size: 81 213 244 884 1779 6736 Fragments arranged by size: 6736 1779 884 244 213 81 EcoO109I rG'GnC_Cy Cuts at: 2107 2318 2637 3147 3148 3410 3576 3699 4297 Size: 211 319 510 1 262 166 123 598 Cuts at: 4297 4755 4983 5257 5359 5773 6142 6190 6560 Size: 458 228 274 102 414 369 48 370 Cuts at: 6560 6561 2107 Size: 1 5483 Fragments arranged by size: 5483 598 510 458 414 370 369 319 274 262 228 211 166 123 102 48 1 1 EcoRI G'AATT_C Cuts at: 4763 7246 7624 4763 Size: 2483 378 7076 Fragments arranged by size: 7076 2483 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2239 2905 3790 2239 Size: 666 885 8386 Fragments arranged by size: 8386 885 666 FspI TGC'GCA Cuts at: 4232 4328 6998 8443 4232 Size: 96 2670 1445 5726 Fragments arranged by size: 5726 2670 1445 96 HaeII r_GCGC'y Cuts at: 1678 3806 4108 5621 6371 9084 1678 Size: 2128 302 1513 750 2713 2531 Fragments arranged by size: 2713 2531 2128 1513 750 302 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2351 2383 3062 3094 3531 Size: 32 135 32 779 32 679 32 437 Cuts at: 3531 3563 3672 3704 4769 4801 5924 5956 6377 Size: 32 109 32 1065 32 1123 32 421 Cuts at: 6377 6409 7048 7080 8578 8610 8652 8684 1373 Size: 32 639 32 1498 32 42 32 2626 Fragments arranged by size: 2626 1498 1123 1065 779 679 639 437 421 135 109 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 7281 7527 669 Size: 988 281 5343 246 3079 Fragments arranged by size: 5343 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9937 HpaI GTT'AAC Cuts at: 7527 7527 Size: 9937 KasI G'GCGC_C Cuts at: 5617 6367 5617 Size: 750 9187 KpnI G_GTAC'C Cuts at: 2479 7262 2479 Size: 4783 5154 MfeI C'AATT_G Cuts at: 7536 7536 Size: 9937 MluI A'CGCG_T Cuts at: 9813 9813 Size: 9937 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2703 4291 5087 5781 6323 9051 9235 1305 Size: 1398 1588 796 694 542 2728 184 2007 Fragments arranged by size: 2728 2007 1588 1398 796 694 542 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2463 3668 4469 6455 6743 Size: 54 1425 343 631 1205 801 1986 288 Cuts at: 6743 10 Size: 3204 Fragments arranged by size: 3204 1986 1425 1205 801 631 343 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 2531 2781 4322 5660 7895 8254 8413 Size: 648 1365 250 1541 1338 2235 359 159 Cuts at: 8413 518 Size: 2042 Fragments arranged by size: 2235 2042 1541 1365 1338 648 359 250 159 MspA1I CmG'CkG Cuts at: 1606 2332 2467 3953 4159 4549 4727 5582 6040 Size: 726 135 1486 206 390 178 855 458 Cuts at: 6040 6331 6983 7174 7353 8030 9178 9423 1606 Size: 291 652 191 179 677 1148 245 2120 Fragments arranged by size: 2120 1486 1148 855 726 677 652 458 390 291 245 206 191 179 178 135 NaeI GCC'GGC Cuts at: 6657 6657 Size: 9937 NarI GG'CG_CC Cuts at: 5618 6368 5618 Size: 750 9187 NcoI C'CATG_G Cuts at: 513 1065 2348 3649 4429 513 Size: 552 1283 1301 780 6021 Fragments arranged by size: 6021 1301 1283 780 552 NdeI CA'TA_TG Cuts at: 387 2845 387 Size: 2458 7479 NgoMIV G'CCGG_C Cuts at: 6655 6655 Size: 9937 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9937 NotI GC'GGCC_GC Cuts at: 7384 7384 Size: 9937 NspI r_CATG'y Cuts at: 1132 1229 2584 5167 5389 7295 8840 1132 Size: 97 1355 2583 222 1906 1545 2229 Fragments arranged by size: 2583 2229 1906 1545 1355 222 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9937 PciI A'CATG_T Cuts at: 8836 8836 Size: 9937 PflMI CCAn_nnn'nTGG Cuts at: 1108 2335 3200 3613 3664 5140 1108 Size: 1227 865 413 51 1476 5905 Fragments arranged by size: 5905 1476 1227 865 413 51 PfoI T'CCnGG_A Cuts at: 2133 2598 2961 3258 5355 5472 6162 7206 2133 Size: 465 363 297 2097 117 690 1044 4864 Fragments arranged by size: 4864 2097 1044 690 465 363 297 117 PmeI GTTT'AAAC Cuts at: 7241 7241 Size: 9937 PmlI CAC'GTG Cuts at: 4419 4609 4419 Size: 190 9747 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8006 8038 9519 9551 8006 Size: 32 1481 32 8392 Fragments arranged by size: 8392 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2107 2637 3576 4297 4755 5257 5359 6142 6190 Size: 530 939 721 458 502 102 783 48 Cuts at: 6190 2107 Size: 5854 Fragments arranged by size: 5854 939 783 721 530 502 458 102 48 PshAI GACnn'nnGTC Cuts at: 1613 3587 5364 1613 Size: 1974 1777 6186 Fragments arranged by size: 6186 1974 1777 PsiI TTA'TAA Cuts at: 7507 7507 Size: 9937 PspOMI G'GGCC_C Cuts at: 3147 6560 3147 Size: 3413 6524 PspXI vC'TCGA_Gb Cuts at: 4749 5025 7088 4749 Size: 276 2063 7598 Fragments arranged by size: 7598 2063 276 PstI C_TGCA'G Cuts at: 830 2061 3031 3165 3339 3355 3838 4290 4800 Size: 1231 970 134 174 16 483 452 510 Cuts at: 4800 5307 6039 6081 6312 6330 6825 7289 830 Size: 507 732 42 231 18 495 464 3478 Fragments arranged by size: 3478 1231 970 732 510 507 495 483 464 452 231 174 134 42 18 16 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9937 PvuII CAG'CTG Cuts at: 2332 4549 5582 6040 6331 2332 Size: 2217 1033 458 291 5938 Fragments arranged by size: 5938 2217 1033 458 291 SacI G_AGCT'C Cuts at: 3075 3723 3075 Size: 648 9289 SalI G'TCGA_C Cuts at: 1936 7279 1936 Size: 5343 4594 SanDI GG'GwC_CC Cuts at: 2107 2107 Size: 9937 SapI GCTCTTCn'nnn_ Cuts at: 2282 2408 4232 2282 Size: 126 1824 7987 Fragments arranged by size: 7987 1824 126 SbfI CC_TGCA'GG Cuts at: 4290 7289 4290 Size: 2999 6938 ScaI AGT'ACT Cuts at: 8185 9897 8185 Size: 1712 8225 SfcI C'TryA_G Cuts at: 826 1045 2057 3027 3161 3335 3351 3834 4286 Size: 219 1012 970 134 174 16 483 452 Cuts at: 4286 4796 5303 6035 6077 6194 6308 6326 6821 Size: 510 507 732 42 117 114 18 495 Cuts at: 6821 6838 7285 8420 9101 9292 826 Size: 17 447 1135 681 191 1471 Fragments arranged by size: 1471 1135 1012 970 732 681 510 507 495 483 452 447 219 191 174 134 117 114 42 18 17 16 SfiI GGCCn_nnn'nGGCC Cuts at: 4260 4260 Size: 9937 SfoI GGC'GCC Cuts at: 5619 6369 5619 Size: 750 9187 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9937 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9937 SmaI CCC'GGG Cuts at: 1872 3415 6602 7264 1872 Size: 1543 3187 662 4545 Fragments arranged by size: 4545 3187 1543 662 SmlI C'TyrA_G Cuts at: 820 1017 1943 4749 5014 5025 7088 8041 8942 Size: 197 926 2806 265 11 2063 953 901 Cuts at: 8942 9204 820 Size: 262 1553 Fragments arranged by size: 2806 2063 1553 953 926 901 265 262 197 11 SnaBI TAC'GTA Cuts at: 493 7728 493 Size: 7235 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9937 SphI G_CATG'C Cuts at: 1132 5389 7295 1132 Size: 4257 1906 3774 Fragments arranged by size: 4257 3774 1906 SspI AAT'ATT Cuts at: 5 52 4386 7052 7861 5 Size: 47 4334 2666 809 2081 Fragments arranged by size: 4334 2666 2081 809 47 StuI AGG'CCT Cuts at: 1517 3167 4458 1517 Size: 1650 1291 6996 Fragments arranged by size: 6996 1650 1291 StyI C'CwwG_G Cuts at: 513 1065 2348 3108 3168 3649 3813 4014 4429 Size: 552 1283 760 60 481 164 201 415 Cuts at: 4429 4788 4854 4875 5127 5613 5890 6087 6285 Size: 359 66 21 252 486 277 197 198 Cuts at: 6285 6372 6966 7376 513 Size: 87 594 410 3074 Fragments arranged by size: 3074 1283 760 594 552 486 481 415 410 359 277 252 201 198 197 164 87 66 60 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2705 3577 3675 5558 6569 6577 6962 Size: 1255 1125 872 98 1883 1011 8 385 Cuts at: 6962 7964 7981 8134 8319 8764 325 Size: 1002 17 153 185 445 1498 Fragments arranged by size: 1883 1498 1255 1125 1011 1002 872 445 385 185 153 98 17 8 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2577 5688 6228 Size: 274 80 33 51 1443 600 3111 540 Cuts at: 6228 8183 9895 96 Size: 1955 1712 138 Fragments arranged by size: 3111 1955 1712 1443 600 540 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2142 2174 4383 4415 4613 4645 4767 4799 5101 Size: 32 2209 32 198 32 122 32 302 Cuts at: 5101 5133 5725 5757 9897 9929 2142 Size: 32 592 32 4140 32 2150 Fragments arranged by size: 4140 2209 2150 592 302 198 122 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6587 1316 Size: 5271 4666 XbaI T'CTAG_A Cuts at: 7273 7273 Size: 9937 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2530 3385 5053 5620 2530 Size: 855 1668 567 6847 Fragments arranged by size: 6847 1668 855 567 XhoI C'TCGA_G Cuts at: 1943 4749 5025 7088 1943 Size: 2806 276 2063 4792 Fragments arranged by size: 4792 2806 2063 276 XmaI C'CCGG_G Cuts at: 1870 3413 6600 7262 1870 Size: 1543 3187 662 4545 Fragments arranged by size: 4545 3187 1543 662 XmnI GAAnn'nnTTC Cuts at: 2885 6017 8066 2885 Size: 3132 2049 4756 Fragments arranged by size: 4756 3132 2049 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6466 7745 276 Size: 53 83 186 936 4932 1279 2468 Fragments arranged by size: 4932 2468 1279 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII SacII SexAI SrfI SwaI