(Circular) (Six-base) MAPSORT of: pFN21AB1311.seq Check: 1930 from: 1 to: 8857 pFN21AB1311 8857 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2706 2706 Size: 8857 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6667 278 Size: 53 83 186 936 5131 2468 Fragments arranged by size: 5131 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5232 6200 1937 Size: 3295 968 4594 Fragments arranged by size: 4594 3295 968 Acc65I G'GTAC_C Cuts at: 3079 3312 4859 5172 6178 3079 Size: 233 1547 313 1006 5758 Fragments arranged by size: 5758 1547 1006 313 233 AclI AA'CG_TT Cuts at: 1563 5238 6984 7357 1563 Size: 3675 1746 373 3063 Fragments arranged by size: 3675 3063 1746 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2590 3281 3848 4426 5245 5251 5342 5997 Size: 958 691 567 578 819 6 91 655 Cuts at: 5997 6174 6920 8304 8572 1632 Size: 177 746 1384 268 1917 Fragments arranged by size: 1917 1384 958 819 746 691 655 578 567 268 177 91 6 AfeI AGC'GCT Cuts at: 2858 4145 4934 5052 5603 2858 Size: 1287 789 118 551 6112 Fragments arranged by size: 6112 1287 789 551 118 AflII C'TTAA_G Cuts at: 820 1017 4769 820 Size: 197 3752 4908 Fragments arranged by size: 4908 3752 197 AflIII A'CryG_T Cuts at: 1203 2297 2595 3264 3614 3645 4947 4949 5391 Size: 1094 298 669 350 31 1302 2 442 Cuts at: 5391 7756 8733 1203 Size: 2365 977 1327 Fragments arranged by size: 2365 1327 1302 1094 977 669 442 350 298 31 2 AgeI A'CCGG_T Cuts at: 7688 7688 Size: 8857 AhdI GACnn_n'nnGTC Cuts at: 7586 7586 Size: 8857 AleI CACnn'nnGTG Cuts at: 1166 2756 1166 Size: 1590 7267 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3545 5045 1184 Size: 2361 1500 4996 Fragments arranged by size: 4996 2361 1500 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4352 4384 4352 Size: 32 8825 AlwNI CAG_nnn'CTG Cuts at: 6020 6104 6136 6600 8172 6020 Size: 84 32 464 1572 6705 Fragments arranged by size: 6705 1572 464 84 32 ApaI G_GGCC'C Cuts at: 4490 4490 Size: 8857 ApaLI G'TGCA_C Cuts at: 6914 8070 6914 Size: 1156 7701 ApoI r'AATT_y Cuts at: 1493 2830 3346 4351 6166 6392 6544 8653 1493 Size: 1337 516 1005 1815 226 152 2109 1697 Fragments arranged by size: 2109 1815 1697 1337 1005 516 226 152 AseI AT'TA_AT Cuts at: 160 1032 7411 160 Size: 872 6379 1606 Fragments arranged by size: 6379 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8857 AvaI C'yCGr_G Cuts at: 1870 1943 2678 3906 4459 4539 5125 5168 6111 Size: 73 735 1228 553 80 586 43 943 Cuts at: 6111 6182 1870 Size: 71 4545 Fragments arranged by size: 4545 1228 943 735 586 553 80 73 71 43 BamHI G'GATC_C Cuts at: 2203 2247 4137 6187 6554 2203 Size: 44 1890 2050 367 4506 Fragments arranged by size: 4506 2050 1890 367 44 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 2654 2927 3024 3079 Size: 325 217 627 333 534 273 97 55 Cuts at: 3079 3312 4503 4629 4859 5021 5172 5378 5782 Size: 233 1191 126 230 162 151 206 404 Cuts at: 5782 6178 7633 618 Size: 396 1455 1842 Fragments arranged by size: 1842 1455 1191 627 534 404 396 333 325 273 233 230 217 206 162 151 126 97 55 BanII G_rGCy'C Cuts at: 1391 2273 4490 5945 5964 6176 1391 Size: 882 2217 1455 19 212 4072 Fragments arranged by size: 4072 2217 1455 882 212 19 BbeI G_GCGC'C Cuts at: 2124 2658 2931 3028 4507 2124 Size: 534 273 97 1479 6474 Fragments arranged by size: 6474 1479 534 273 97 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2627 4459 4543 928 Size: 971 728 1832 84 5242 Fragments arranged by size: 5242 1832 971 728 84 BbvCI CC'TCA_GC Cuts at: 3597 5712 6067 3597 Size: 2115 355 6387 Fragments arranged by size: 6387 2115 355 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2017 2051 3471 3505 5912 5946 7048 Size: 34 80 34 1420 34 2407 34 1102 Cuts at: 7048 7082 1903 Size: 34 3678 Fragments arranged by size: 3678 2407 1420 1102 80 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4279 6749 7959 4279 Size: 2470 1210 5177 Fragments arranged by size: 5177 2470 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8857 BfrBI ATG'CAT Cuts at: 2011 3965 2011 Size: 1954 6903 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2502 5203 7468 Size: 107 122 71 631 605 830 2701 2265 Cuts at: 7468 136 Size: 1525 Fragments arranged by size: 2701 2265 1525 830 631 605 122 107 71 BglII A'GATC_T Cuts at: 8852 8852 Size: 8857 BlpI GC'TnA_GC Cuts at: 3245 5087 5703 6274 3245 Size: 1842 616 571 5828 Fragments arranged by size: 5828 1842 616 571 Bme1580I G_kGCm'C Cuts at: 1790 2361 2454 2695 3129 3988 4490 4891 5024 Size: 571 93 241 434 859 502 401 133 Cuts at: 5024 5214 5381 6918 8074 1790 Size: 190 167 1537 1156 2573 Fragments arranged by size: 2573 1537 1156 859 571 502 434 401 241 190 167 133 93 BmgBI CAC'GTC Cuts at: 1318 2596 5768 1318 Size: 1278 3172 4407 Fragments arranged by size: 4407 3172 1278 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2299 4126 4210 Size: 416 53 83 399 24 882 1827 84 Cuts at: 4210 4306 6588 7546 442 Size: 96 2282 958 1753 Fragments arranged by size: 2282 1827 1753 958 882 416 399 96 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5711 1056 Size: 4655 4202 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4879 5272 4879 Size: 393 8464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 2705 4853 6045 6075 6158 7517 1239 Size: 965 501 2148 1192 30 83 1359 2579 Fragments arranged by size: 2579 2148 1359 1192 965 501 83 30 Bpu10I CC'TnA_GC Cuts at: 3597 5712 5790 6067 3597 Size: 2115 78 277 6387 Fragments arranged by size: 6387 2115 277 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3518 4816 5651 6982 7847 8145 3518 Size: 1298 835 1331 865 298 4230 Fragments arranged by size: 4230 1331 1298 865 835 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2050 3418 3719 5260 7520 882 Size: 617 551 1368 301 1541 2260 2219 Fragments arranged by size: 2260 2219 1541 1368 617 551 301 BsaAI yAC'GTr Cuts at: 493 1204 2671 3232 4950 5392 6648 493 Size: 711 1467 561 1718 442 1256 2702 Fragments arranged by size: 2702 1718 1467 1256 711 561 442 BsaBI GATnn'nnATC Cuts at: 2208 2909 8851 2208 Size: 701 5942 2214 Fragments arranged by size: 5942 2214 701 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2121 2655 2928 3025 Size: 53 83 186 936 588 534 273 97 Cuts at: 3025 4504 4777 6664 7046 7681 275 Size: 1479 273 1887 382 635 1451 Fragments arranged by size: 1887 1479 1451 936 635 588 534 382 273 273 186 97 83 53 BsaWI w'CCGG_w Cuts at: 2244 3432 4164 5460 7290 7688 7962 8109 8520 Size: 1188 732 1296 1830 398 274 147 411 Cuts at: 8520 2244 Size: 2581 Fragments arranged by size: 2581 1830 1296 1188 732 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2907 2937 3479 3509 4438 Size: 30 165 30 1504 30 542 30 929 Cuts at: 4438 4468 5238 5268 6031 6061 6134 6164 1178 Size: 30 770 30 763 30 73 30 3871 Fragments arranged by size: 3871 1504 929 770 763 542 165 73 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2116 2636 4115 5264 6155 1186 Size: 913 17 520 1479 1149 891 3888 Fragments arranged by size: 3888 1479 1149 913 891 520 17 BseYI C'CCAG_C Cuts at: 2061 3223 3308 4002 4128 5374 5887 6073 6079 Size: 1162 85 694 126 1246 513 186 6 Cuts at: 6079 6097 8060 8556 2061 Size: 18 1963 496 2362 Fragments arranged by size: 2362 1963 1246 1162 694 513 496 186 126 85 18 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 2399 2627 2690 2873 3655 4371 Size: 573 417 246 228 63 183 782 716 Cuts at: 4371 4858 1163 Size: 487 5162 Fragments arranged by size: 5162 782 716 573 487 417 246 228 183 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2323 3286 4348 6307 7068 8096 Size: 275 186 322 963 1062 1959 761 1028 Cuts at: 8096 1540 Size: 2301 Fragments arranged by size: 2301 1959 1062 1028 963 761 322 275 186 BsiHKAI G_wGCw'C Cuts at: 5337 5913 6918 7003 8074 5337 Size: 576 1005 85 1071 6120 Fragments arranged by size: 6120 1071 1005 576 85 BsmI GAATG_Cn' Cuts at: 3509 3967 6368 6461 3509 Size: 458 2401 93 5905 Fragments arranged by size: 5905 2401 458 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4768 4768 Size: 8857 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2273 2361 2454 2695 3129 3988 4490 Size: 399 483 88 93 241 434 859 502 Cuts at: 4490 4891 5024 5214 5337 5381 5913 5945 5964 Size: 401 133 190 123 44 532 32 19 Cuts at: 5964 6176 6918 7003 8074 1391 Size: 212 742 85 1071 2174 Fragments arranged by size: 2174 1071 859 742 532 502 483 434 401 399 241 212 190 133 123 93 88 85 44 32 19 BspEI T'CCGG_A Cuts at: 3432 8520 3432 Size: 5088 3769 BspHI T'CATG_A Cuts at: 6746 8476 6746 Size: 1730 7127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2421 2706 3247 3737 6212 844 Size: 1577 285 541 490 2475 3489 Fragments arranged by size: 3489 2475 1577 541 490 285 BsrBI CCG'CTC Cuts at: 1394 1823 3282 3931 4296 6744 1394 Size: 429 1459 649 365 2448 3507 Fragments arranged by size: 3507 2448 1459 649 429 365 BsrDI GCAATG_nn' Cuts at: 65 1248 7352 7526 65 Size: 1183 6104 174 1396 Fragments arranged by size: 6104 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3576 4683 5091 7501 7688 1700 Size: 110 1766 1107 408 2410 187 2869 Fragments arranged by size: 2869 2410 1766 1107 408 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8857 BssHII G'CGCG_C Cuts at: 1925 2432 1925 Size: 507 8350 BssSI C'ACGA_G Cuts at: 2568 3338 5975 6917 7929 2568 Size: 770 2637 942 1012 3496 Fragments arranged by size: 3496 2637 1012 942 770 BstAPI GCAn_nnn'nTGC Cuts at: 794 2800 794 Size: 2006 6851 BstBI TT'CG_AA Cuts at: 7661 7661 Size: 8857 BstEII G'GTnAC_C Cuts at: 1184 2187 5216 1184 Size: 1003 3029 4825 Fragments arranged by size: 4825 3029 1003 BstXI CCAn_nnnn'nTGG Cuts at: 5000 5000 Size: 8857 BstYI r'GATC_y Cuts at: 1972 2203 2247 4137 4757 5595 6132 6187 6554 Size: 231 44 1890 620 838 537 55 367 Cuts at: 6554 6939 6956 8408 8494 8506 8852 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1890 1452 838 620 537 385 367 346 231 86 55 44 17 12 Bsu36I CC'TnA_GG Cuts at: 2486 4322 2486 Size: 1836 7021 BtgI C'CryG_G Cuts at: 513 1065 2040 2192 2256 2965 3142 3514 4912 Size: 552 975 152 64 709 177 372 1398 Cuts at: 4912 5161 5221 513 Size: 249 60 4149 Fragments arranged by size: 4149 1398 975 709 552 372 249 177 152 64 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2838 2989 3256 5915 487 Size: 683 56 1612 151 267 2659 3429 Fragments arranged by size: 3429 2659 1612 683 267 151 56 BtsI GCAGTG_nn' Cuts at: 2427 3823 4056 4709 6373 7167 7187 2427 Size: 1396 233 653 1664 794 20 4097 Fragments arranged by size: 4097 1664 1396 794 653 233 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8857 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8822 DraI TTT'AAA Cuts at: 6161 6161 Size: 8857 DraIII CAC_nnn'GTG Cuts at: 2800 3143 3343 2800 Size: 343 200 8314 Fragments arranged by size: 8314 343 200 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7864 809 Size: 507 6548 1802 Fragments arranged by size: 6548 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 2818 3015 Size: 54 1425 50 275 18 429 559 197 Cuts at: 3015 3145 3283 3932 4090 5093 5425 6304 7193 Size: 130 138 649 158 1003 332 879 889 Cuts at: 7193 8822 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1003 889 879 649 559 429 332 275 197 158 138 130 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3283 6304 1537 Size: 275 1471 3021 4090 Fragments arranged by size: 4090 3021 1471 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2027 5474 5918 6787 1353 Size: 674 3447 444 869 3423 Fragments arranged by size: 3447 3423 869 674 444 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2784 5269 7444 7818 7964 387 Size: 1064 138 1195 2485 2175 374 146 1280 Fragments arranged by size: 2485 2175 1280 1195 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2590 2705 3281 3848 4426 4853 Size: 393 572 386 115 576 567 578 427 Cuts at: 4853 5245 5251 5342 5997 6045 6075 6158 6174 Size: 392 6 91 655 48 30 83 16 Cuts at: 6174 6920 7517 8304 8572 1239 Size: 746 597 787 268 1524 Fragments arranged by size: 1524 787 746 655 597 578 576 572 567 427 393 392 386 268 115 91 83 48 30 16 6 EcoO109I rG'GnC_Cy Cuts at: 2096 3104 3181 3441 3601 4446 4486 4487 5352 Size: 1008 77 260 160 845 40 1 865 Cuts at: 5352 5716 6017 6028 2096 Size: 364 301 11 4925 Fragments arranged by size: 4925 1008 865 845 364 301 260 160 77 40 11 1 EcoRI G'AATT_C Cuts at: 6166 6544 6166 Size: 378 8479 EcoRV GAT'ATC Cuts at: 3853 5369 3853 Size: 1516 7341 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5692 5692 Size: 8857 FspI TGC'GCA Cuts at: 7363 7363 Size: 8857 HaeII r_GCGC'y Cuts at: 1678 2124 2658 2860 2931 3028 4147 4507 4936 Size: 446 534 202 71 97 1119 360 429 Cuts at: 4936 5054 5605 8004 1678 Size: 118 551 2399 2531 Fragments arranged by size: 2531 2399 1119 551 534 446 429 360 202 118 97 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2188 2220 2537 2569 3479 Size: 32 135 32 616 32 317 32 910 Cuts at: 3479 3511 4438 4470 5238 5270 6134 6166 7498 Size: 32 927 32 768 32 864 32 1332 Cuts at: 7498 7530 7572 7604 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1332 927 910 864 768 616 317 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3001 3262 3548 3556 6201 6447 Size: 988 281 1063 261 286 8 2645 246 Cuts at: 6447 669 Size: 3079 Fragments arranged by size: 3079 2645 1063 988 286 281 261 246 8 HindIII A'AGCT_T Cuts at: 748 3293 748 Size: 2545 6312 HpaI GTT'AAC Cuts at: 6447 6447 Size: 8857 KasI G'GCGC_C Cuts at: 2120 2654 2927 3024 4503 2120 Size: 534 273 97 1479 6474 Fragments arranged by size: 6474 1479 534 273 97 KpnI G_GTAC'C Cuts at: 3083 3316 4863 5176 6182 3083 Size: 233 1547 313 1006 5758 Fragments arranged by size: 5758 1547 1006 313 233 MfeI C'AATT_G Cuts at: 6456 6456 Size: 8857 MluI A'CGCG_T Cuts at: 8733 8733 Size: 8857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3522 5514 7971 8155 1305 Size: 2217 1992 2457 184 2007 Fragments arranged by size: 2457 2217 2007 1992 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 5427 10 Size: 54 1425 343 429 3166 3440 Fragments arranged by size: 3440 3166 1425 429 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2756 2903 4727 5027 5330 5477 5955 Size: 648 1590 147 1824 300 303 147 478 Cuts at: 5955 6815 7174 7333 518 Size: 860 359 159 2042 Fragments arranged by size: 2042 1824 1590 860 648 478 359 303 300 159 147 147 MspA1I CmG'CkG Cuts at: 1606 2042 2334 2967 4923 5362 5374 6273 6950 Size: 436 292 633 1956 439 12 899 677 Cuts at: 6950 8098 8343 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1956 1148 899 677 633 439 436 292 245 12 NaeI GCC'GGC Cuts at: 3578 5093 3578 Size: 1515 7342 NarI GG'CG_CC Cuts at: 2121 2655 2928 3025 4504 2121 Size: 534 273 97 1479 6474 Fragments arranged by size: 6474 1479 534 273 97 NcoI C'CATG_G Cuts at: 513 1065 3514 4912 5161 513 Size: 552 2449 1398 249 4209 Fragments arranged by size: 4209 2449 1398 552 249 NdeI CA'TA_TG Cuts at: 387 387 Size: 8857 NgoMIV G'CCGG_C Cuts at: 3576 5091 3576 Size: 1515 7342 NheI G'CTAG_C Cuts at: 1052 5707 1052 Size: 4655 4202 NotI GC'GGCC_GC Cuts at: 6304 6304 Size: 8857 NsiI A_TGCA'T Cuts at: 2013 3967 2013 Size: 1954 6903 NspI r_CATG'y Cuts at: 1132 1229 2308 3618 3649 5086 6215 7760 1132 Size: 97 1079 1310 31 1437 1129 1545 2229 Fragments arranged by size: 2229 1545 1437 1310 1129 1079 97 31 PasI CC'CwG_GG Cuts at: 1348 2116 2659 4689 1348 Size: 768 543 2030 5516 Fragments arranged by size: 5516 2030 768 543 PciI A'CATG_T Cuts at: 3614 3645 7756 3614 Size: 31 4111 4715 Fragments arranged by size: 4715 4111 31 PflMI CCAn_nnn'nTGG Cuts at: 1108 2198 3143 3483 4423 6104 6136 1108 Size: 1090 945 340 940 1681 32 3829 Fragments arranged by size: 3829 1681 1090 945 940 340 32 PfoI T'CCnGG_A Cuts at: 3032 4355 6135 3032 Size: 1323 1780 5754 Fragments arranged by size: 5754 1780 1323 PmeI GTTT'AAAC Cuts at: 6161 6161 Size: 8857 PmlI CAC'GTG Cuts at: 3232 4950 3232 Size: 1718 7139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4298 4330 5492 5524 6926 6958 8439 8471 4298 Size: 32 1162 32 1402 32 1481 32 4684 Fragments arranged by size: 4684 1481 1402 1162 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2096 3181 3441 3601 4446 5352 6028 2096 Size: 1085 260 160 845 906 676 4925 Fragments arranged by size: 4925 1085 906 845 676 260 160 PshAI GACnn'nnGTC Cuts at: 1613 5579 1613 Size: 3966 4891 PsiI TTA'TAA Cuts at: 6427 6427 Size: 8857 PspOMI G'GGCC_C Cuts at: 4486 4486 Size: 8857 PspXI vC'TCGA_Gb Cuts at: 2678 3906 2678 Size: 1228 7629 PstI C_TGCA'G Cuts at: 830 4063 4716 4765 5065 6209 830 Size: 3233 653 49 300 1144 3478 Fragments arranged by size: 3478 3233 1144 653 300 49 PvuI CG_AT'CG Cuts at: 2001 4348 2001 Size: 2347 6510 PvuII CAG'CTG Cuts at: 2334 5362 5374 2334 Size: 3028 12 5817 Fragments arranged by size: 5817 3028 12 SacII CC_GC'GG Cuts at: 2043 2968 2043 Size: 925 7932 SalI G'TCGA_C Cuts at: 1936 6199 1936 Size: 4263 4594 SanDI GG'GwC_CC Cuts at: 2096 5352 2096 Size: 3256 5601 SapI GCTCTTCn'nnn_ Cuts at: 2027 5918 2027 Size: 3891 4966 SbfI CC_TGCA'GG Cuts at: 6209 6209 Size: 8857 ScaI AGT'ACT Cuts at: 3956 7105 8817 3956 Size: 3149 1712 3996 Fragments arranged by size: 3996 3149 1712 SexAI A'CCwGG_T Cuts at: 2743 4632 2743 Size: 1889 6968 SfcI C'TryA_G Cuts at: 826 1045 2134 4059 4075 4712 4761 4936 5042 Size: 219 1089 1925 16 637 49 175 106 Cuts at: 5042 5061 6039 6205 7340 8021 8212 826 Size: 19 978 166 1135 681 191 1471 Fragments arranged by size: 1925 1471 1135 1089 978 681 637 219 191 175 166 106 49 19 16 SfiI GGCCn_nnn'nGGCC Cuts at: 5203 5203 Size: 8857 SfoI GGC'GCC Cuts at: 2122 2656 2929 3026 4505 2122 Size: 534 273 97 1479 6474 Fragments arranged by size: 6474 1479 534 273 97 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8857 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8857 SmaI CCC'GGG Cuts at: 1872 4541 5127 5170 6113 6184 1872 Size: 2669 586 43 943 71 4545 Fragments arranged by size: 4545 2669 943 586 71 43 SmlI C'TyrA_G Cuts at: 820 1017 1943 2678 3533 3906 4769 4795 5666 Size: 197 926 735 855 373 863 26 871 Cuts at: 5666 6961 7862 8124 820 Size: 1295 901 262 1553 Fragments arranged by size: 1553 1295 926 901 871 863 855 735 373 262 197 26 SnaBI TAC'GTA Cuts at: 493 6648 493 Size: 6155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8857 SphI G_CATG'C Cuts at: 1132 6215 1132 Size: 5083 3774 SspI AAT'ATT Cuts at: 5 52 6781 5 Size: 47 6729 2081 Fragments arranged by size: 6729 2081 47 StuI AGG'CCT Cuts at: 1517 4327 5549 1517 Size: 2810 1222 4825 Fragments arranged by size: 4825 2810 1222 StyI C'CwwG_G Cuts at: 513 1065 3514 4912 5161 5544 5964 6296 513 Size: 552 2449 1398 249 383 420 332 3074 Fragments arranged by size: 3074 2449 1398 552 420 383 332 249 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2337 2633 2826 4882 5435 5557 6884 Size: 1255 757 296 193 2056 553 122 1327 Cuts at: 6884 6901 7054 7239 7684 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2056 1498 1327 1255 757 553 445 296 193 185 153 122 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3843 3954 4188 Size: 274 80 33 51 1443 1866 111 234 Cuts at: 4188 4591 7103 8815 96 Size: 403 2512 1712 138 Fragments arranged by size: 2512 1866 1712 1443 403 274 234 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2907 2939 3790 3822 4380 4412 5892 5924 8817 Size: 32 851 32 558 32 1480 32 2893 Cuts at: 8817 8849 2907 Size: 32 2915 Fragments arranged by size: 2915 2893 1480 851 558 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2291 2745 1316 Size: 975 454 7428 Fragments arranged by size: 7428 975 454 XbaI T'CTAG_A Cuts at: 6193 6193 Size: 8857 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3480 4420 5000 3480 Size: 940 580 7337 Fragments arranged by size: 7337 940 580 XhoI C'TCGA_G Cuts at: 1943 2678 3906 1943 Size: 735 1228 6894 Fragments arranged by size: 6894 1228 735 XmaI C'CCGG_G Cuts at: 1870 4539 5125 5168 6111 6182 1870 Size: 2669 586 43 943 71 4545 Fragments arranged by size: 4545 2669 943 586 71 43 XmnI GAAnn'nnTTC Cuts at: 5468 6986 5468 Size: 1518 7339 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6665 276 Size: 53 83 186 936 5131 2468 Fragments arranged by size: 5131 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BsiWI BstZ17I EcoICRI EcoNI FseI FspAI NruI PacI PsrI RsrII SacI SrfI SwaI