Restriction map for
pFN21AB0065
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# NO CUT ENZYMES ( 81 enzymes) #
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Acc65I AccII AccIII AclI AflII
AgeI Alw44I ApaLI AscI Asp718I
AvaI AviII BbeI BbrPI BcgI
BfrI BsaBI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BspHI BsrBI
BsrFI BssHII Bst1107I BstUI BstZ17I
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco52I EcoNI EcoRI EcoRV
FseI FspI KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 3180 GTCTAC GT^[AC][GT]AC
AflIII 3564 ACATGT A^C[GA][CT]GT
AhdI 4565 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 44 AGCGCT AGC^GCT
AspEI 4565 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5257 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2072 TGGCCA TGG^CCA
BbvCI 676 CCTCAGC CCTCAGC(-5/-2)
BciVI 1548 GTATCC GTATCC(6/5)
BcnI 5194 CCGGG CC^[GC]GG
BglI 3832 GCCGACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1972 CACGTA [CT]AC^GT[GA]
Bsp1407I 162 TGTACA T^GTACA
BspLU11I 3564 ACATGT A^CATGT
BsrGI 162 TGTACA T^GTACA
BssSI 3209 CACGAG CACGAG(-5/-1)
BstBI 3098 TTCGAA TT^CGAA
DraI 4432 TTTAAA TTT^AAA
DsaI 4553 CCACGG C^C[GA][CT]GG
Eam1105I 4565 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 44 AGCGCT AGC^GCT
HaeII 44 AGCGCT [GA]GCGC^[CT]
HgaI 5097 GACGC GACGC(5/10)
HindIII 3297 AAGCTT A^AGCTT
MluNI 2072 TGGCCA TGG^CCA
MscI 2072 TGGCCA TGG^CCA
NciI 5194 CCGGG CC^[GC]GG
NspV 3098 TTCGAA TT^CGAA
PflFI 5257 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 5995 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1776 GCTCTTC GCTCTTC(1/4)
ScaI 2676 AGTACT AGT^ACT
SfuI 3098 TTCGAA TT^CGAA
SphI 33 GCATGC GCATG^C
SspBI 162 TGTACA T^GTACA
Tth111I 5257 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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ApaI 26 GGGCCC GGGCC^C
5514 GGGCCC
AvrII 3051 CCTAGG C^CTAGG
5205 CCTAGG
BlnI 3051 CCTAGG C^CTAGG
5205 CCTAGG
BlpI 2350 GCTAAGC GC^T[ACGT]AGC
3525 GCTAAGC
Bpu1102I 2350 GCTAAGC GC^T[ACGT]AGC
3525 GCTAAGC
BsaWI 744 TCCGGT [AT]^CCGG[AT]
4791 ACCGGA
BsmBI 1787 GAGACG CGTCTC(1/5)
5756 GAGACG
BstEII 2242 GGTGACC G^GT[ACGT]ACC
4615 GGTTACC
BstPI 2242 GGTGACC G^GT[ACGT]ACC
4615 GGTTACC
CelII 2350 GCTAAGC GC^T[ACGT]AGC
3525 GCTAAGC
CfoI 45 GCGC GCG^C
5112 GCGC
DraIII 4505 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5564 CACTTGGTG
EcoO65I 2242 GGTGACC G^GT[ACGT]ACC
4615 GGTTACC
EcoT22I 5465 ATGCAT ATGCA^T
5717 ATGCAT
HhaI 45 GCGC GCG^C
5112 GCGC
HinP1I 45 GCGC G^CGC
5112 GCGC
HincII 2269 GTTAAC GT[CT]^[GA]AC
5701 GTTAAC
HindII 2269 GTTAAC GT[CT]^[GA]AC
5701 GTTAAC
HpaI 2269 GTTAAC GTT^AAC
5701 GTTAAC
MfeI 2159 CAATTG C^AATTG
3432 CAATTG
MunI 2159 CAATTG C^AATTG
3432 CAATTG
NdeI 2689 CATATG CA^TATG
4045 CATATG
NsiI 5465 ATGCAT ATGCA^T
5717 ATGCAT
SexAI 179 ACCAGGT A^CC[AT]GGT
321 ACCTGGT
StuI 438 AGGCCT AGG^CCT
5202 AGGCCT
XbaI 4194 TCTAGA T^CTAGA
6045 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
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AatII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AspHI AvaII
BamHI BanI BanII BbsI BbvI
BclI BfaI BglII BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
Hin1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshBI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI