Restriction map for pFN21AB0065
 (Linear) (Six-base) MAPSORT of: KIBB0065.seq  Check: 9277  from: 1  to: 6123



KIBB0065.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2814    5730    6123 
   Size:       2814    2916     393

  Fragments arranged by size: 

                2916    2814     393
 
AatII G_ACGT'C

Cuts at:      0    1427    5827    6003    6123 
   Size:       1427    4400     176     120

  Fragments arranged by size: 

                4400    1427     176     120
 
AccI GT'mk_AC

Cuts at:      0    3181    6123 
   Size:       3181    2942
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     127     136     182    1190    1291    1556    1764 
   Size:        117      10       9      46    1008     101     265     208

Cuts at:   1764    2332    2445    3774    3890    4666    4697    5892    6011 
   Size:        568     113    1329     116     776      31    1195     119

Cuts at:   6011    6123 
   Size:        112

  Fragments arranged by size: 

                1329    1195    1008     776     568     265     208     119
                 117     116     113     112     101      46      31      10
                   9
 
AfeI AGC'GCT

Cuts at:      0      46    6123 
   Size:         46    6077
 
AflIII A'CryG_T

Cuts at:      0    3564    6123 
   Size:       3564    2559
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4570    6123 
   Size:       4570    1553
 
AleI CACnn'nnGTG

Cuts at:      0    2426    2848    6123 
   Size:       2426     422    3275

  Fragments arranged by size: 

                3275    2426     422
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    5762    5807    6123 
   Size:       5762      45     316

  Fragments arranged by size: 

                5762     316      45
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     482     514    6123 
   Size:        482      32    5609

  Fragments arranged by size: 

                5609     482      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     144     171     322    2332    4587    4720    4761    5989 
   Size:        144      27     151    2010    2255     133      41    1228

Cuts at:   5989    6123 
   Size:        134

  Fragments arranged by size: 

                2255    2010    1228     151     144     134     133      41
                  27
 
ApaI G_GGCC'C

Cuts at:      0      30    5518    6123 
   Size:         30    5488     605

  Fragments arranged by size: 

                5488     605      30
 
ApoI r'AATT_y

Cuts at:      0     361     560    2661    6123 
   Size:        361     199    2101    3462

  Fragments arranged by size: 

                3462    2101     361     199
 
AseI AT'TA_AT

Cuts at:      0     338    3026    3170    5317    6123 
   Size:        338    2688     144    2147     806

  Fragments arranged by size: 

                2688    2147     806     338     144
 
AvrII C'CTAG_G

Cuts at:      0    3051    5205    6123 
   Size:       3051    2154     918

  Fragments arranged by size: 

                3051    2154     918
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    5228    5261    6123 
   Size:       5228      33     862

  Fragments arranged by size: 

                5228     862      33
 
BamHI G'GATC_C

Cuts at:      0    2013    2154    5422    6123 
   Size:       2013     141    3268     701

  Fragments arranged by size: 

                3268    2013     701     141
 
BanI G'GyrC_C

Cuts at:      0    1006    2199    3118    3840    4954    6123 
   Size:       1006    1193     919     722    1114    1169

  Fragments arranged by size: 

                1193    1169    1114    1006     919     722
 
BanII G_rGCy'C

Cuts at:      0      30     127     419    1409    2935    2959    5518    6123 
   Size:         30      97     292     990    1526      24    2559     605

  Fragments arranged by size: 

                2559    1526     990     605     292      97      30      24
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      95    1427    3231    4116    4654    5013    5298    5339 
   Size:         95    1332    1804     885     538     359     285      41

Cuts at:   5339    5999    6123 
   Size:        660     124

  Fragments arranged by size: 

                1804    1332     885     660     538     359     285     124
                  95      41
 
BbvCI CC'TCA_GC

Cuts at:      0     677    6123 
   Size:        677    5446
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1559    6123 
   Size:       1559    4564
 
BclI T'GATC_A

Cuts at:      0     366     555     588     870    3930    6123 
   Size:        366     189      33     282    3060    2193

  Fragments arranged by size: 

                3060    2193     366     282     189      33
 
BfrBI ATG'CAT

Cuts at:      0    5467    5719    6123 
   Size:       5467     252     404

  Fragments arranged by size: 

                5467     404     252
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3838    6123 
   Size:       3838    2285
 
BglII A'GATC_T

Cuts at:      0     924    1977    5027    6123 
   Size:        924    1053    3050    1096

  Fragments arranged by size: 

                3050    1096    1053     924
 
BlpI GC'TnA_GC

Cuts at:      0    2351    3526    6123 
   Size:       2351    1175    2597

  Fragments arranged by size: 

                2597    2351    1175
 
Bme1580I G_kGCm'C

Cuts at:      0      30    2488    3893    4263    5518    6123 
   Size:         30    2458    1405     370    1255     605

  Fragments arranged by size: 

                2458    1405    1255     605     370      30
 
BmgBI CAC'GTC

Cuts at:      0    5760    6123 
   Size:       5760     363
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3839    4749    5539    5825    6123 
   Size:       3839     910     790     286     298

  Fragments arranged by size: 

                3839     910     790     298     286
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455     878    2128    2719    3483    3632    4078    4471 
   Size:        455     423    1250     591     764     149     446     393

Cuts at:   4471    5039    5261    5567    5627    5771    6123 
   Size:        568     222     306      60     144     352

  Fragments arranged by size: 

                1250     764     591     568     455     446     423     393
                 352     306     222     149     144      60
 
Bpu10I CC'TnA_GC

Cuts at:      0     677    2393    4921    5901    6123 
   Size:        677    1716    2528     980     222

  Fragments arranged by size: 

                2528    1716     980     677     222
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2311    2549    5073    6123 
   Size:       2311     238    2524    1050

  Fragments arranged by size: 

                2524    2311    1050     238
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     890    1268    5300    6123 
   Size:        890     378    4032     823

  Fragments arranged by size: 

                4032     890     823     378
 
BsaAI yAC'GTr

Cuts at:      0    1974    6123 
   Size:       1974    4149
 
BsaHI Gr'CG_yC

Cuts at:      0    1424    5098    5824    6000    6123 
   Size:       1424    3674     726     176     123

  Fragments arranged by size: 

                3674    1424     726     176     123
 
BsaWI w'CCGG_w

Cuts at:      0     744    4791    6123 
   Size:        744    4047    1332

  Fragments arranged by size: 

                4047    1332     744
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    5143    5173    5234    5264    5744    5774    6123 
   Size:       5143      30      61      30     480      30     349

  Fragments arranged by size: 

                5143     480     349      61      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     409     494     875    1044    1395    2630    5164    5564 
   Size:        409      85     381     169     351    1235    2534     400

Cuts at:   5564    5768    5849    6123 
   Size:        204      81     274

  Fragments arranged by size: 

                2534    1235     409     400     381     351     274     204
                 169      85      81
 
BseYI C'CCAG_C

Cuts at:      0     243     486    4093    4914    4950    6123 
   Size:        243     243    3607     821      36    1173

  Fragments arranged by size: 

                3607    1173     821     243     243      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2912    3748    3903    4745    6123 
   Size:       2912     836     155     842    1378

  Fragments arranged by size: 

                2912    1378     842     836     155
 
BsiHKAI G_wGCw'C

Cuts at:      0     419    1367    1409    2322    2959    6123 
   Size:        419     948      42     913     637    3164

  Fragments arranged by size: 

                3164     948     913     637     419      42
 
BsmI GAATG_Cn'

Cuts at:      0    2090    4242    5469    6123 
   Size:       2090    2152    1227     654

  Fragments arranged by size: 

                2152    2090    1227     654
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1781    5750    6123 
   Size:       1781    3969     373

  Fragments arranged by size: 

                3969    1781     373
 
Bsp1286I G_dGCh'C

Cuts at:      0      30     127     419    1367    1409    2322    2488    2935 
   Size:         30      97     292     948      42     913     166     447

Cuts at:   2935    2959    3893    4263    5518    6123 
   Size:         24     934     370    1255     605

  Fragments arranged by size: 

                1255     948     934     913     605     447     370     292
                 166      97      42      30      24
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     357    2814    2951    4864    5730    6123 
   Size:        357    2457     137    1913     866     393

  Fragments arranged by size: 

                2457    1913     866     393     357     137
 
BsrDI GCAATG_nn'

Cuts at:      0     670     976    1169    2210    3537    5597    6023    6123 
   Size:        670     306     193    1041    1327    2060     426     100

  Fragments arranged by size: 

                2060    1327    1041     670     426     306     193     100
            
 
BsrGI T'GTAC_A

Cuts at:      0     162    6123 
   Size:        162    5961
 
BssSI C'ACGA_G

Cuts at:      0    3209    6123 
   Size:       3209    2914
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1006    1033    4723    4837    6123 
   Size:       1006      27    3690     114    1286

  Fragments arranged by size: 

                3690    1286    1006     114      27
 
BstBI TT'CG_AA

Cuts at:      0    3099    6123 
   Size:       3099    3024
 
BstEII G'GTnAC_C

Cuts at:      0    2242    4615    6123 
   Size:       2242    2373    1508

  Fragments arranged by size: 

                2373    2242    1508
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     535    1038    3453    6123 
   Size:        535     503    2415    2670

  Fragments arranged by size: 

                2670    2415     535     503
 
BstYI r'GATC_y

Cuts at:      0     790     924    1977    2013    2154    5027    5422    6123 
   Size:        790     134    1053      36     141    2873     395     701

  Fragments arranged by size: 

                2873    1053     790     701     395     141     134      36
            
 
Bsu36I CC'TnA_GG

Cuts at:      0     964    2645    3483    6123 
   Size:        964    1681     838    2640

  Fragments arranged by size: 

                2640    1681     964     838
 
BtgI C'CryG_G

Cuts at:      0    4553    6123 
   Size:       4553    1570
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2041    5526    6123 
   Size:       2041    3485     597

  Fragments arranged by size: 

                3485    2041     597
 
BtsI GCAGTG_nn'

Cuts at:      0     525     897     948    1031    1245    3891    4310    6123 
   Size:        525     372      51      83     214    2646     419    1813

  Fragments arranged by size: 

                2646    1813     525     419     372     214      83      51
            
 
DraI TTT'AAA

Cuts at:      0    4434    6123 
   Size:       4434    1689
 
DraIII CAC_nnn'GTG

Cuts at:      0    4510    5569    6123 
   Size:       4510    1059     554

  Fragments arranged by size: 

                4510    1059     554
 
EaeI y'GGCC_r

Cuts at:      0    2072    3135    4316    6123 
   Size:       2072    1063    1181    1807

  Fragments arranged by size: 

                2072    1807    1181    1063
 
EarI CTCTTCn'nnn_

Cuts at:      0     136    1783    2048    2464    2588    3170    3278    3312 
   Size:        136    1647     265     416     124     582     108      34

Cuts at:   3312    3380    6123 
   Size:         68    2743

  Fragments arranged by size: 

                2743    1647     582     416     265     136     124     108
                  68      34
 
EcoICRI GAG'CTC

Cuts at:      0     417    1407    2957    6123 
   Size:        417     990    1550    3166

  Fragments arranged by size: 

                3166    1550     990     417
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     127     136     182     455     878    1190    1291 
   Size:        117      10       9      46     273     423     312     101

Cuts at:   1291    1556    1764    2128    2332    2445    2719    3483    3632 
   Size:        265     208     364     204     113     274     764     149

Cuts at:   3632    3774    3890    4078    4471    4666    4697    5039    5261 
   Size:        142     116     188     393     195      31     342     222

Cuts at:   5261    5567    5627    5771    5892    6011    6123 
   Size:        306      60     144     121     119     112

  Fragments arranged by size: 

                 764     423     393     364     342     312     306     274
                 273     265     222     208     204     195     188     149
                 144     142     121     119     117     116     113     112
                 101      60      46      31      10       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0      27     319    1753    2770    5136    5514    5515    5854 
   Size:         27     292    1434    1017    2366     378       1     339

Cuts at:   5854    6123 
   Size:        269

  Fragments arranged by size: 

                2366    1434    1017     378     339     292     269      27
                   1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3804    6123 
   Size:       3804    2319
 
HaeII r_GCGC'y

Cuts at:      0      48    6123 
   Size:         48    6075
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     243     275     794     826    1396    1428    1486    1518 
   Size:        243      32     519      32     570      32      58      32

Cuts at:   1518    1998    2030    2847    2879    3218    3250    3504    3536 
   Size:        480      32     817      32     339      32     254      32

Cuts at:   3536    3605    3637    4191    4223    4556    4588    5012    5044 
   Size:         69      32     554      32     333      32     424      32

Cuts at:   5044    6123 
   Size:       1079

  Fragments arranged by size: 

                1079     817     570     554     519     480     424     339
                 333     254     243      69      58      32      32      32
                  32      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    2271    5703    6123 
   Size:       2271    3432     420

  Fragments arranged by size: 

                3432    2271     420
 
HindIII A'AGCT_T

Cuts at:      0    3297    6123 
   Size:       3297    2826
 
HpaI GTT'AAC

Cuts at:      0    2271    5703    6123 
   Size:       2271    3432     420

  Fragments arranged by size: 

                3432    2271     420
 
MfeI C'AATT_G

Cuts at:      0    2159    3432    6123 
   Size:       2159    1273    2691

  Fragments arranged by size: 

                2691    2159    1273
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1740    4554    5898    6123 
   Size:       1740    2814    1344     225

  Fragments arranged by size: 

                2814    1740    1344     225
 
MscI TGG'CCA

Cuts at:      0    2074    6123 
   Size:       2074    4049
 
MslI CAynn'nnrTG

Cuts at:      0     338     533    1838    2426    2764    2848    3116    4235 
   Size:        338     195    1305     588     338      84     268    1119

Cuts at:   4235    5734    6109    6123 
   Size:       1499     375      14

  Fragments arranged by size: 

                1499    1305    1119     588     375     338     338     268
                 195      84      14
 
MspA1I CmG'CkG

Cuts at:      0     171     247    1564    1769    2923    3586    3998    4414 
   Size:        171      76    1317     205    1154     663     412     416

Cuts at:   4414    4720    6123 
   Size:        306    1403

  Fragments arranged by size: 

                1403    1317    1154     663     416     412     306     205
                 171      76
 
NdeI CA'TA_TG

Cuts at:      0    2690    4046    6123 
   Size:       2690    1356    2077

  Fragments arranged by size: 

                2690    2077    1356
 
NsiI A_TGCA'T

Cuts at:      0    5469    5721    6123 
   Size:       5469     252     402

  Fragments arranged by size: 

                5469     402     252
 
NspI r_CATG'y

Cuts at:      0      37    3568    4997    6123 
   Size:         37    3531    1429    1126

  Fragments arranged by size: 

                3531    1429    1126      37
 
PasI CC'CwG_GG

Cuts at:      0     390    6123 
   Size:        390    5733
 
PciI A'CATG_T

Cuts at:      0    3564    6123 
   Size:       3564    2559
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     295    1842    4777    5419    6123 
   Size:        295    1547    2935     642     704

  Fragments arranged by size: 

                2935    1547     704     642     295
 
PfoI T'CCnGG_A

Cuts at:      0    4111    4326    6123 
   Size:       4111     215    1797

  Fragments arranged by size: 

                4111    1797     215
 
PpuMI rG'GwC_Cy

Cuts at:      0     319    1753    5854    6123 
   Size:        319    1434    4101     269

  Fragments arranged by size: 

                4101    1434     319     269
 
PshAI GACnn'nnGTC

Cuts at:      0    5999    6123 
   Size:       5999     124
 
PsiI TTA'TAA

Cuts at:      0    2185    3727    6123 
   Size:       2185    1542    2396

  Fragments arranged by size: 

                2396    2185    1542
 
PspOMI G'GGCC_C

Cuts at:      0      26    5514    6123 
   Size:         26    5488     609

  Fragments arranged by size: 

                5488     609      26
 
PstI C_TGCA'G

Cuts at:      0     122     629     717    3273    4158    5186    6123 
   Size:        122     507      88    2556     885    1028     937

  Fragments arranged by size: 

                2556    1028     937     885     507     122      88
 
PvuII CAG'CTG

Cuts at:      0     171     247    1564    2923    3586    3998    4414    4720 
   Size:        171      76    1317    1359     663     412     416     306

Cuts at:   4720    6123 
   Size:       1403

  Fragments arranged by size: 

                1403    1359    1317     663     416     412     306     171
                  76
 
SacI G_AGCT'C

Cuts at:      0     419    1409    2959    6123 
   Size:        419     990    1550    3164

  Fragments arranged by size: 

                3164    1550     990     419
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1783    6123 
   Size:       1783    4340
 
ScaI AGT'ACT

Cuts at:      0    2678    6123 
   Size:       2678    3445
 
SexAI A'CCwGG_T

Cuts at:      0     179     321    6123 
   Size:        179     142    5802

  Fragments arranged by size: 

                5802     179     142
 
SfcI C'TryA_G

Cuts at:      0     118     625     713    1868    2300    3269    3701    4154 
   Size:        118     507      88    1155     432     969     432     453

Cuts at:   4154    4592    5124    5182    6123 
   Size:        438     532      58     941

  Fragments arranged by size: 

                1155     969     941     532     507     453     438     432
                 432     118      88      58
 
SmlI C'TyrA_G

Cuts at:      0    2290    2564    5052    6123 
   Size:       2290     274    2488    1071

  Fragments arranged by size: 

                2488    2290    1071     274
 
SphI G_CATG'C

Cuts at:      0      37    6123 
   Size:         37    6086
 
SspI AAT'ATT

Cuts at:      0     189     213     494    6123 
   Size:        189      24     281    5629

  Fragments arranged by size: 

                5629     281     189      24
 
StuI AGG'CCT

Cuts at:      0     440    5204    6123 
   Size:        440    4764     919

  Fragments arranged by size: 

                4764     919     440
 
StyI C'CwwG_G

Cuts at:      0     441    1152    3051    3736    4460    5205    6123 
   Size:        441     711    1899     685     724     745     918

  Fragments arranged by size: 

                1899     918     745     724     711     685     441
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1044    1923    1930    2261    3858    4121    4942    5365 
   Size:       1044     879       7     331    1597     263     821     423

Cuts at:   5365    5615    5707    6073    6123 
   Size:        250      92     366      50

  Fragments arranged by size: 

                1597    1044     879     821     423     366     331     263
                 250      92      50       7
 
TatI w'GTAC_w

Cuts at:      0     162    2047    2676    3782    5428    6123 
   Size:        162    1885     629    1106    1646     695

  Fragments arranged by size: 

                1885    1646    1106     695     629     162
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1346    1378    5143    5175    5234    5266    5744    5776 
   Size:       1346      32    3765      32      59      32     478      32

Cuts at:   5776    6123 
   Size:        347

  Fragments arranged by size: 

                3765    1346     478     347      59      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    5260    6123 
   Size:       5260     863
 
XbaI T'CTAG_A

Cuts at:      0    4194    6045    6123 
   Size:       4194    1851      78

  Fragments arranged by size: 

                4194    1851      78
 
XmnI GAAnn'nnTTC

Cuts at:      0    1777    2057    3228    3680    4367    5336    6123 
   Size:       1777     280    1171     452     687     969     787

  Fragments arranged by size: 

                1777    1171     969     787     687     452     280
 
ZraI GAC'GTC

Cuts at:      0    1425    5825    6001    6123 
   Size:       1425    4400     176     122

  Fragments arranged by size: 

                4400    1425     176     122
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI     ApoI     AseI    AvrII     BaeI
    BamHI     BanI    BanII     BbsI    BbvCI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrDI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI   DraIII     EaeI     EarI  EcoICRI  Eco57MI
 EcoO109I     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     MfeI
     MmeI     MscI     MslI   MspA1I     NdeI     NsiI     NspI     PasI
     PciI    PflMI     PfoI    PpuMI    PshAI     PsiI   PspOMI     PstI
    PvuII     SacI     SapI     ScaI    SexAI     SfcI     SmlI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI    ApaLI     AscI    AsiSI     AvaI
     BbeI     BcgI     BmtI     BplI    BsaBI    BsiEI    BsiWI    BspEI
    BspHI    BsrBI    BsrFI   BssHII  BstZ17I     ClaI    CspCI     DrdI
     EagI     EciI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
     KasI     KpnI     MluI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     PacI     PmeI     PmlI     PpiI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI     XcmI
     XhoI     XmaI
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