Restriction map for
pFN21AB0048
(Linear) (Six-base) MAPSORT of: KIBB0048.seq Check: 9936 from: 1 to: 5268
KIBB0048.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AccI GT'mk_AC
Cuts at: 0 1726 2475 3471 5268
Size: 1726 749 996 1797
Fragments arranged by size:
1797 1726 996 749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 3485 3848 3985 3995 4028 4199 4802
Size: 179 3306 363 137 10 33 171 603
Cuts at: 4802 5107 5268
Size: 305 161
Fragments arranged by size:
3306 603 363 305 179 171 161 137
33 10
AhdI GACnn_n'nnGTC
Cuts at: 0 483 918 5139 5169 5268
Size: 483 435 4221 30 99
Fragments arranged by size:
4221 483 435 99 30
AleI CACnn'nnGTG
Cuts at: 0 529 5268
Size: 529 4739
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3074 3106 5268
Size: 3074 32 2162
Fragments arranged by size:
3074 2162 32
AlwNI CAG_nnn'CTG
Cuts at: 0 337 846 3451 5268
Size: 337 509 2605 1817
Fragments arranged by size:
2605 1817 509 337
ApaI G_GGCC'C
Cuts at: 0 3512 5268
Size: 3512 1756
ApoI r'AATT_y
Cuts at: 0 593 836 998 1240 1361 2289 2811 4143
Size: 593 243 162 242 121 928 522 1332
Cuts at: 4143 4362 4603 5268
Size: 219 241 665
Fragments arranged by size:
1332 928 665 593 522 243 242 241
219 162 121
AvaI C'yCGr_G
Cuts at: 0 2989 3124 4871 5268
Size: 2989 135 1747 397
Fragments arranged by size:
2989 1747 397 135
BamHI G'GATC_C
Cuts at: 0 2979 3938 5268
Size: 2979 959 1330
Fragments arranged by size:
2979 1330 959
BanI G'GyrC_C
Cuts at: 0 3408 5268
Size: 3408 1860
BanII G_rGCy'C
Cuts at: 0 51 1630 2840 3512 5268
Size: 51 1579 1210 672 1756
Fragments arranged by size:
1756 1579 1210 672 51
BbsI GAAGACnn'nnnn_
Cuts at: 0 553 1774 2501 4646 5197 5268
Size: 553 1221 727 2145 551 71
Fragments arranged by size:
2145 1221 727 553 551 71
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2398 2432 3105 3139 5268
Size: 2398 34 673 34 2129
Fragments arranged by size:
2398 2129 673 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1596 2138 5268
Size: 1596 542 3130
Fragments arranged by size:
3130 1596 542
BclI T'GATC_A
Cuts at: 0 933 1044 1356 2526 5268
Size: 933 111 312 1170 2742
Fragments arranged by size:
2742 1170 933 312 111
BfrBI ATG'CAT
Cuts at: 0 2628 5268
Size: 2628 2640
BglII A'GATC_T
Cuts at: 0 3787 5268
Size: 3787 1481
BlpI GC'TnA_GC
Cuts at: 0 1703 1832 5268
Size: 1703 129 3436
Fragments arranged by size:
3436 1703 129
Bme1580I G_kGCm'C
Cuts at: 0 3050 3512 5268
Size: 3050 462 1756
Fragments arranged by size:
3050 1756 462
BmrI ACTGGGnnnn_n'
Cuts at: 0 1633 2115 4483 5268
Size: 1633 482 2368 785
Fragments arranged by size:
2368 1633 785 482
BmtI G_CTAG'C
Cuts at: 0 1714 5268
Size: 1714 3554
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2634 5268
Size: 2634 2634
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1788 2423 2729 2851 3294 3827 4511 5268
Size: 1788 635 306 122 443 533 684 757
Fragments arranged by size:
1788 757 684 635 533 443 306 122
Bpu10I CC'TnA_GC
Cuts at: 0 4445 5268
Size: 4445 823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3138 3281 4925 5062 5268
Size: 3138 143 1644 137 206
Fragments arranged by size:
3138 1644 206 143 137
BsaI GGTCTCn'nnnn_
Cuts at: 0 1745 3113 3796 5268
Size: 1745 1368 683 1472
Fragments arranged by size:
1745 1472 1368 683
BsaAI yAC'GTr
Cuts at: 0 3099 5268
Size: 3099 2169
BsaBI GATnn'nnATC
Cuts at: 0 1481 1967 2984 3792 5268
Size: 1481 486 1017 808 1476
Fragments arranged by size:
1481 1476 1017 808 486
BsaWI w'CCGG_w
Cuts at: 0 977 2571 3480 5268
Size: 977 1594 909 1788
Fragments arranged by size:
1788 1594 977 909
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1244 1274 1433 1463 2634 2664 2832 2862
Size: 1244 30 159 30 1171 30 168 30
Cuts at: 2862 3074 3104 3292 3322 4227 4257 4509 4539
Size: 212 30 188 30 905 30 252 30
Cuts at: 4539 5268
Size: 729
Fragments arranged by size:
1244 1171 905 729 252 212 188 168
159 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 471 2607 2848 4235 4238 5157 5268
Size: 471 2136 241 1387 3 919 111
Fragments arranged by size:
2136 1387 919 471 241 111 3
BseYI C'CCAG_C
Cuts at: 0 1222 2775 2844 3488 4271 5268
Size: 1222 1553 69 644 783 997
Fragments arranged by size:
1553 1222 997 783 644 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4064 4619 5268
Size: 4064 555 649
Fragments arranged by size:
4064 649 555
BsiHKAI G_wGCw'C
Cuts at: 0 3873 4326 5268
Size: 3873 453 942
Fragments arranged by size:
3873 942 453
BsmI GAATG_Cn'
Cuts at: 0 2630 5268
Size: 2630 2638
BsmBI CGTCTCn'nnnn_
Cuts at: 0 815 1988 4304 5268
Size: 815 1173 2316 964
Fragments arranged by size:
2316 1173 964 815
Bsp1286I G_dGCh'C
Cuts at: 0 51 1630 2840 3050 3512 3873 4326 5268
Size: 51 1579 1210 210 462 361 453 942
Fragments arranged by size:
1579 1210 942 462 453 361 210 51
BspEI T'CCGG_A
Cuts at: 0 977 5268
Size: 977 4291
BspHI T'CATG_A
Cuts at: 0 1200 2631 5268
Size: 1200 1431 2637
Fragments arranged by size:
2637 1431 1200
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3586 5268
Size: 3586 1682
BsrBI CCG'CTC
Cuts at: 0 1831 5268
Size: 1831 3437
BsrDI GCAATG_nn'
Cuts at: 0 197 972 3743 4085 4252 4896 5268
Size: 197 775 2771 342 167 644 372
Fragments arranged by size:
2771 775 644 372 342 197 167
BsrFI r'CCGG_y
Cuts at: 0 896 5268
Size: 896 4372
BsrGI T'GTAC_A
Cuts at: 0 1610 1719 3932 5268
Size: 1610 109 2213 1336
Fragments arranged by size:
2213 1610 1336 109
BssSI C'ACGA_G
Cuts at: 0 1991 5268
Size: 1991 3277
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4087 5268
Size: 4087 1181
BstBI TT'CG_AA
Cuts at: 0 1644 1806 5268
Size: 1644 162 3462
Fragments arranged by size:
3462 1644 162
BstYI r'GATC_y
Cuts at: 0 2955 2979 3729 3787 3938 5214 5268
Size: 2955 24 750 58 151 1276 54
Fragments arranged by size:
2955 1276 750 151 58 54 24
BstZ17I GTA'TAC
Cuts at: 0 1727 5268
Size: 1727 3541
BtgI C'CryG_G
Cuts at: 0 469 3615 5070 5268
Size: 469 3146 1455 198
Fragments arranged by size:
3146 1455 469 198
BtsI GCAGTG_nn'
Cuts at: 0 3691 5038 5268
Size: 3691 1347 230
Fragments arranged by size:
3691 1347 230
ClaI AT'CG_AT
Cuts at: 0 4539 5268
Size: 4539 729
DraIII CAC_nnn'GTG
Cuts at: 0 4330 5116 5268
Size: 4330 786 152
Fragments arranged by size:
4330 786 152
EaeI y'GGCC_r
Cuts at: 0 387 427 3271 4862 5268
Size: 387 40 2844 1591 406
Fragments arranged by size:
2844 1591 406 387 40
EarI CTCTTCn'nnn_
Cuts at: 0 986 1112 3320 3356 3431 3716 4823 5268
Size: 986 126 2208 36 75 285 1107 445
Fragments arranged by size:
2208 1107 986 445 285 126 75 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1763 2392 3411 5268
Size: 1763 629 1019 1857
Fragments arranged by size:
1857 1763 1019 629
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 1788 2423 2729 2851 3294 3485 3827
Size: 179 1609 635 306 122 443 191 342
Cuts at: 3827 3848 3985 3995 4028 4199 4511 4802 5107
Size: 21 137 10 33 171 312 291 305
Cuts at: 5107 5268
Size: 161
Fragments arranged by size:
1609 635 443 342 312 306 305 291
191 179 171 161 137 122 33 21
10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3503 5268
Size: 3503 1765
EcoO109I rG'GnC_Cy
Cuts at: 0 2516 3508 5268
Size: 2516 992 1760
Fragments arranged by size:
2516 1760 992
EcoRI G'AATT_C
Cuts at: 0 4362 5268
Size: 4362 906
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1359 1432 5268
Size: 1359 73 3836
Fragments arranged by size:
3836 1359 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 904 936 1667 1699 1810 1842
Size: 469 32 403 32 731 32 111 32
Cuts at: 1842 2178 2210 2407 2439 3292 3324 5049 5081
Size: 336 32 197 32 853 32 1725 32
Cuts at: 5081 5125 5155 5157 5187 5268
Size: 44 30 2 30 81
Fragments arranged by size:
1725 853 731 469 403 336 197 111
81 44 32 32 32 32 32 32
32 32 30 30 2
HincII GTy'rAC
Cuts at: 0 198 405 5268
Size: 198 207 4863
Fragments arranged by size:
4863 207 198
HindIII A'AGCT_T
Cuts at: 0 2735 5268
Size: 2735 2533
HpaI GTT'AAC
Cuts at: 0 198 405 5268
Size: 198 207 4863
Fragments arranged by size:
4863 207 198
MfeI C'AATT_G
Cuts at: 0 2132 5268
Size: 2132 3136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1272 2983 5186 5211 5268
Size: 1272 1711 2203 25 57
Fragments arranged by size:
2203 1711 1272 57 25
MscI TGG'CCA
Cuts at: 0 4864 5268
Size: 4864 404
MslI CAynn'nnrTG
Cuts at: 0 529 1040 1205 5268
Size: 529 511 165 4063
Fragments arranged by size:
4063 529 511 165
MspA1I CmG'CkG
Cuts at: 0 157 471 918 2352 3628 3639 4038 4822
Size: 157 314 447 1434 1276 11 399 784
Cuts at: 4822 5268
Size: 446
Fragments arranged by size:
1434 1276 784 447 446 399 314 157
11
NcoI C'CATG_G
Cuts at: 0 3615 5070 5268
Size: 3615 1455 198
Fragments arranged by size:
3615 1455 198
NheI G'CTAG_C
Cuts at: 0 1710 5268
Size: 1710 3558
NsiI A_TGCA'T
Cuts at: 0 2630 5268
Size: 2630 2638
NspI r_CATG'y
Cuts at: 0 229 1039 2074 2953 5268
Size: 229 810 1035 879 2315
Fragments arranged by size:
2315 1035 879 810 229
PasI CC'CwG_GG
Cuts at: 0 3043 5268
Size: 3043 2225
PflMI CCAn_nnn'nTGG
Cuts at: 0 385 967 1177 3451 4495 5268
Size: 385 582 210 2274 1044 773
Fragments arranged by size:
2274 1044 773 582 385 210
PfoI T'CCnGG_A
Cuts at: 0 51 538 5208 5268
Size: 51 487 4670 60
Fragments arranged by size:
4670 487 60 51
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3074 3106 5268
Size: 3074 32 2162
Fragments arranged by size:
3074 2162 32
PspOMI G'GGCC_C
Cuts at: 0 3508 5268
Size: 3508 1760
PspXI vC'TCGA_Gb
Cuts at: 0 3124 5268
Size: 3124 2144
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1295 1327 2880 2912 5268
Size: 1295 32 1553 32 2356
Fragments arranged by size:
2356 1553 1295 32 32
PstI C_TGCA'G
Cuts at: 0 1332 3522 3627 5268
Size: 1332 2190 105 1641
Fragments arranged by size:
2190 1641 1332 105
PvuII CAG'CTG
Cuts at: 0 157 2352 3628 4038 4822 5268
Size: 157 2195 1276 410 784 446
Fragments arranged by size:
2195 1276 784 446 410 157
SacII CC_GC'GG
Cuts at: 0 472 5268
Size: 472 4796
SapI GCTCTTCn'nnn_
Cuts at: 0 3356 3431 5268
Size: 3356 75 1837
Fragments arranged by size:
3356 1837 75
ScaI AGT'ACT
Cuts at: 0 2898 5268
Size: 2898 2370
SfcI C'TryA_G
Cuts at: 0 169 1328 3518 3623 5268
Size: 169 1159 2190 105 1645
Fragments arranged by size:
2190 1645 1159 169 105
SmlI C'TyrA_G
Cuts at: 0 3124 3153 3296 4940 5077 5268
Size: 3124 29 143 1644 137 191
Fragments arranged by size:
3124 1644 191 143 137 29
SnaBI TAC'GTA
Cuts at: 0 3099 5268
Size: 3099 2169
SphI G_CATG'C
Cuts at: 0 1039 5268
Size: 1039 4229
SspI AAT'ATT
Cuts at: 0 630 1298 3547 4024 5268
Size: 630 668 2249 477 1244
Fragments arranged by size:
2249 1244 668 630 477
StuI AGG'CCT
Cuts at: 0 882 1899 5268
Size: 882 1017 3369
Fragments arranged by size:
3369 1017 882
StyI C'CwwG_G
Cuts at: 0 1334 3576 3615 5070 5268
Size: 1334 2242 39 1455 198
Fragments arranged by size:
2242 1455 1334 198 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 197 3056 3977 4067 5268
Size: 197 2859 921 90 1201
Fragments arranged by size:
2859 1201 921 197 90
TatI w'GTAC_w
Cuts at: 0 1610 1719 2896 3753 3932 5268
Size: 1610 109 1177 857 179 1336
Fragments arranged by size:
1610 1336 1177 857 179 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1244 1276 1433 1465 2832 2864 4227 4259
Size: 1244 32 157 32 1367 32 1363 32
Cuts at: 4259 5104 5136 5268
Size: 845 32 132
Fragments arranged by size:
1367 1363 1244 845 157 132 32 32
32 32 32
XbaI T'CTAG_A
Cuts at: 0 1314 3589 5268
Size: 1314 2275 1679
Fragments arranged by size:
2275 1679 1314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1230 1941 2017 5268
Size: 1230 711 76 3251
Fragments arranged by size:
3251 1230 711 76
XhoI C'TCGA_G
Cuts at: 0 3124 5268
Size: 3124 2144
XmnI GAAnn'nnTTC
Cuts at: 0 2330 3907 5268
Size: 2330 1577 1361
Fragments arranged by size:
2330 1577 1361
Enzymes that do cut:
AccI AcuI AhdI AleI AloI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BstZ17I BtgI BtsI ClaI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII
HindIII HpaI MfeI MmeI MscI MslI MspA1I NcoI
NheI NsiI NspI PasI PflMI PfoI PpiI PspOMI
PspXI PsrI PstI PvuII SacII SapI ScaI SfcI
SmlI SnaBI SphI SspI StuI StyI TaqII TatI
TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BglI BmgBI BsaHI BsiEI BsiWI BssHII BstEII
BstXI Bsu36I BtgZI CspCI DraI DrdI EagI EcoICRI
EcoRV FseI FspI FspAI HaeII KasI KpnI MluI
NaeI NarI NdeI NgoMIV NotI NruI PacI PciI
PmeI PmlI PpuMI PshAI PsiI PvuI RsrII SacI
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SpeI SrfI SwaI Tth111I XmaI ZraI