Restriction map for
pFN21AB0009
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# NO CUT ENZYMES ( 56 enzymes) #
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AatII Acc65I AccI AclI AgeI
AscI AseI AsnI Asp718I BbeI
BciVI BsaBI BsiEI BsiWI BspDI
BssHII Bst1107I BstBI BstZ17I ClaI
DrdI EagI EclXI Eco52I EcoRV
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NarI NdeI
NotI NruI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
SacII SalI ScaI SfiI SfoI
SfuI SgrAI SpeI SspI SwaI
XcmI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 4442 TCCGGA T^CCGGA
AflII 3160 CTTAAG C^TTAAG
AhdI 4426 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3385 AGCGCT AGC^GCT
ApaI 1721 GGGCCC GGGCC^C
AspEI 4426 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3109 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3684 CCTAGG C^CTAGG
BbrPI 4579 CACGTG CAC^GTG
BbvCI 3190 GCTGAGG CCTCAGC(-5/-2)
BclI 2036 TGATCA T^GATCA
BfrI 3160 CTTAAG C^TTAAG
BlnI 3684 CCTAGG C^CTAGG
BseAI 4442 TCCGGA T^CCGGA
BsmBI 448 GAGACG CGTCTC(1/5)
Bsp1407I 1632 TGTACA T^GTACA
BspEI 4442 TCCGGA T^CCGGA
BspHI 195 TCATGA T^CATGA
BspLU11I 1922 ACATGT A^CATGT
BsrGI 1632 TGTACA T^GTACA
CpoI 5 CGGACCG CG^G[AT]CCG
DraI 4740 TTTAAA TTT^AAA
DraIII 1513 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4426 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3385 AGCGCT AGC^GCT
EcoRI 3499 GAATTC G^AATTC
EcoT22I 4342 ATGCAT ATGCA^T
FbaI 2036 TGATCA T^GATCA
FseI 2877 GGCCGGCC GGCCGG^CC
MroI 4442 TCCGGA T^CCGGA
NaeI 2878 GCCGGC GCC^GGC
NcoI 1662 CCATGG C^CATGG
NgoMIV 2878 GCCGGC G^CCGGC
NsiI 4342 ATGCAT ATGCA^T
PaeR7I 610 CTCGAG C^TCGAG
PflFI 3109 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4579 CACGTG CAC^GTG
PmlI 4579 CACGTG CAC^GTG
RcaI 195 TCATGA T^CATGA
RsrII 5 CGGACCG CG^G[AT]CCG
SbfI 2841 CCTGCAGG CCTGCA^GG
SexAI 1514 ACCAGGT A^CC[AT]GGT
SnaBI 1767 TACGTA TAC^GTA
Sse8387I 2841 CCTGCAGG CCTGCA^GG
SspBI 1632 TGTACA T^GTACA
Tth111I 3109 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2070 TCTAGA T^CTAGA
XhoI 610 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 35 enzymes) #
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AccII 605 CGCG CG^CG
3313 CGCG
AcyI 2356 GACGCC G[GA]^CG[CT]C
3769 GACGCC
AflIII 263 ACGTGT A^C[GA][CT]GT
1922 ACATGT
Alw44I 592 GTGCAC G^TGCAC
1510 GTGCAC
AocI 3645 CCTGAGG CC^T[ACGT]AGG
4036 CCTGAGG
ApaLI 592 GTGCAC G^TGCAC
1510 GTGCAC
BglI 1415 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2983 GCCTCTGTGGC
BlpI 1270 GCTGAGC GC^T[ACGT]AGC
3388 GCTAAGC
Bpu1102I 1270 GCTGAGC GC^T[ACGT]AGC
3388 GCTAAGC
BsaAI 1767 TACGTA [CT]AC^GT[GA]
4579 CACGTG
BsaHI 2356 GACGCC G[GA]^CG[CT]C
3769 GACGCC
BsaI 3457 GAGACC GGTCTC(1/5)
3886 GAGACC
BsaWI 3050 ACCGGA [AT]^CCGG[AT]
4442 TCCGGA
BsmI 1086 GAATGC GAATGC(1/-1)
3186 GAATGC
BsrBI 2278 GAGCGG CCGCTC(-3/-3)
4456 GAGCGG
BsrDI 4860 CATTGC GCAATG(2/0)
2720 GCAATG
BstEII 1389 GGTGACC G^GT[ACGT]ACC
3609 GGTGACC
BstPI 1389 GGTGACC G^GT[ACGT]ACC
3609 GGTGACC
BstUI 605 CGCG CG^CG
3313 CGCG
Bsu36I 3645 CCTGAGG CC^T[ACGT]AGG
4036 CCTGAGG
CelII 1270 GCTGAGC GC^T[ACGT]AGC
3388 GCTAAGC
Eco81I 3645 CCTGAGG CC^T[ACGT]AGG
4036 CCTGAGG
EcoNI 1131 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1977 CCTTCAGAAGG
EcoO65I 1389 GGTGACC G^GT[ACGT]ACC
3609 GGTGACC
Hin1I 2356 GACGCC G[GA]^CG[CT]C
3769 GACGCC
HincII 1096 GTCAAC GT[CT]^[GA]AC
3691 GTCAAC
HindII 1096 GTCAAC GT[CT]^[GA]AC
3691 GTCAAC
MvnI 605 CGCG CG^CG
3313 CGCG
NheI 630 GCTAGC G^CTAGC
2454 GCTAGC
PflMI 822 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1515 CCAGGTGCTGG
SapI 1200 GAAGAGC GCTCTTC(1/4)
939 GCTCTTC
SmaI 804 CCCGGG CCC^GGG
1718 CCCGGG
Van91I 822 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1515 CCAGGTGCTGG
XmaCI 804 CCCGGG C^CCGGG
1718 CCCGGG
XmaI 804 CCCGGG C^CCGGG
1718 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII AviII BalI BamHI BanI
BanII BbsI BbvI BcgI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI