Restriction map for pFN21AB0009
 (Circular) (Six-base) MAPSORT of: pFN21AB0009.seq  Check: 2015  from: 1  to: 9667



pFN21AB0009  9667 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2610    2895    3382    3634    2610 
   Size:        285     487     252    8643

  Fragments arranged by size: 

                8643     487     285     252
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    7477     278 
   Size:         53      83     186     936    5941    2468

  Fragments arranged by size: 

                5941    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    7010    1937 
   Size:       5073    4594
 
Acc65I G'GTAC_C

Cuts at:   6988    6988 
   Size:       9667
 
AclI AA'CG_TT

Cuts at:   1563    7794    8167    1563 
   Size:       6231     373    3063

  Fragments arranged by size: 

                6231    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2738    3968    4716    4976    6272    6504    6743    7730 
   Size:       1106    1230     748     260    1296     232     239     987

Cuts at:   7730    9114    9382    1632 
   Size:       1384     268    1917

  Fragments arranged by size: 

                1917    1384    1296    1230    1106     987     748     268
                 260     239     232
 
AfeI AGC'GCT

Cuts at:   5392    5392 
   Size:       9667
 
AflII C'TTAA_G

Cuts at:    820    1017    5165     820 
   Size:        197    4148    5322

  Fragments arranged by size: 

                5322    4148     197
 
AflIII A'CryG_T

Cuts at:   1203    2268    3927    8566    9543    1203 
   Size:       1065    1659    4639     977    1327

  Fragments arranged by size: 

                4639    1659    1327    1065     977
 
AgeI A'CCGG_T

Cuts at:   8498    8498 
   Size:       9667
 
AhdI GACnn_n'nnGTC

Cuts at:   6436    8396    6436 
   Size:       1960    7707
 
AleI CACnn'nnGTG

Cuts at:   1166    4839    1166 
   Size:       3673    5994
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    4857    1184 
   Size:       3673    5994
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3464    3496    4259    4291    3464 
   Size:         32     763      32    8840

  Fragments arranged by size: 

                8840     763      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   2758    2833    2887    3286    3526    3610    4019    4039    4435 
   Size:         75      54     399     240      84     409      20     396

Cuts at:   4435    4507    4711    5485    7410    8982    2758 
   Size:         72     204     774    1925    1572    3443

  Fragments arranged by size: 

                3443    1925    1572     774     409     399     396     240
                 204      84      75      72      54      20
 
ApaI G_GGCC'C

Cuts at:   3730    3730 
   Size:       9667
 
ApaLI G'TGCA_C

Cuts at:   2597    3515    7724    8880    2597 
   Size:        918    4209    1156    3384

  Fragments arranged by size: 

                4209    3384    1156     918
 
ApoI r'AATT_y

Cuts at:   1493    2441    4601    5429    5504    5825    6080    6605    6976 
   Size:        948    2160     828      75     321     255     525     371

Cuts at:   6976    7202    7354    9463    1493 
   Size:        226     152    2109    1697

  Fragments arranged by size: 

                2160    2109    1697     948     828     525     371     321
                 255     226     152      75
 
AseI AT'TA_AT

Cuts at:    160    1032    8221     160 
   Size:        872    7189    1606

  Fragments arranged by size: 

                7189    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       9667
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2615    2809    3723    3839    4148    5259    5267 
   Size:         73     672     194     914     116     309    1111       8

Cuts at:   5267    6992    1870 
   Size:       1725    4545

  Fragments arranged by size: 

                4545    1725    1111     914     672     309     194     116
                  73       8
 
AvrII C'CTAG_G

Cuts at:   5689    5689 
   Size:       9667
 
BamHI G'GATC_C

Cuts at:   4719    5412    5940    6997    7364    4719 
   Size:        693     528    1057     367    7022

  Fragments arranged by size: 

                7022    1057     693     528     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2836    5512    6149    6930    6988 
   Size:        325     217     627    1049    2676     637     781      58

Cuts at:   6988    8443     618 
   Size:       1455    1842

  Fragments arranged by size: 

                2676    1842    1455    1049     781     637     627     325
                 217      58
 
BanII G_rGCy'C

Cuts at:   1391    2312    2672    3467    3724    3730    4158    4635    5301 
   Size:        921     360     795     257       6     428     477     666

Cuts at:   5301    6294    6986    1391 
   Size:        993     692    4072

  Fragments arranged by size: 

                4072     993     921     795     692     666     477     428
                 360     257       6
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2226    2426    3616    3984    4116    4380    4835 
   Size:        971     327     200    1190     368     132     264     455

Cuts at:   4835    4971     928 
   Size:        136    5624

  Fragments arranged by size: 

                5624    1190     971     455     368     327     264     200
                 136     132
 
BbvCI CC'TCA_GC

Cuts at:   5196    5196 
   Size:       9667
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2939    2973    3226    3260    4883    4917    5730 
   Size:         34    1002      34     253      34    1623      34     813

Cuts at:   5730    5764    7858    7892    1903 
   Size:         34    2094      34    3678

  Fragments arranged by size: 

                3678    2094    1623    1002     813     253      34      34
                  34      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   7559    8769    7559 
   Size:       1210    8457
 
BclI T'GATC_A

Cuts at:   1548    4041    1548 
   Size:       2493    7174
 
BfrBI ATG'CAT

Cuts at:   6349    6349 
   Size:       9667
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3426    4994    8278 
   Size:        107     122      71     631     605    1754    1568    3284

Cuts at:   8278     136 
   Size:       1525

  Fragments arranged by size: 

                3284    1754    1568    1525     631     605     122     107
                  71
 
BglII A'GATC_T

Cuts at:   3177    3846    5982    6483    9662    3177 
   Size:        669    2136     501    3179    3182

  Fragments arranged by size: 

                3182    3179    2136     669     501
 
BlpI GC'TnA_GC

Cuts at:   3276    5394    7084    3276 
   Size:       2118    1690    5859

  Fragments arranged by size: 

                5859    2118    1690
 
Bme1580I G_kGCm'C

Cuts at:   1790    2601    2841    3519    3730    4846    6152    7728    8884 
   Size:        811     240     678     211    1116    1306    1576    1156

Cuts at:   8884    1790 
   Size:       2573

  Fragments arranged by size: 

                2573    1576    1306    1156    1116     811     678     240
                 211
 
BmgBI CAC'GTC

Cuts at:   1318    2269    3532    1318 
   Size:        951    1263    7453

  Fragments arranged by size: 

                7453    1263     951
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    5235    7398    8356 
   Size:        416      53      83     399      24    3818    2163     958

Cuts at:   8356     442 
   Size:       1753

  Fragments arranged by size: 

                3818    2163    1753     958     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    2639    4463    1056 
   Size:       1583    1824    6260

  Fragments arranged by size: 

                6260    1824    1583
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2594    2609    2990    3038    3587    3717    3890    4673 
   Size:       1355      15     381      48     549     130     173     783

Cuts at:   4673    4862    5054    5478    5699    5741    5831    6041    6158 
   Size:        189     192     424     221      42      90     210     117

Cuts at:   6158    6326    6392    6963    8327    1239 
   Size:        168      66     571    1364    2579

  Fragments arranged by size: 

                2579    1364    1355     783     571     549     424     381
                 221     210     192     189     173     168     130     117
                  90      66      48      42      15
 
Bpu10I CC'TnA_GC

Cuts at:   3461    4127    5196    3461 
   Size:        666    1069    7932

  Fragments arranged by size: 

                7932    1069     666
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2687    2900    5192    6278    7792    8657    8955    2687 
   Size:        213    2292    1086    1514     865     298    3399

  Fragments arranged by size: 

                3399    2292    1514    1086     865     298     213
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    5456    5885    8330     882 
   Size:        617    3957     429    2445    2219

  Fragments arranged by size: 

                3957    2445    2219     617     429
 
BsaAI yAC'GTr

Cuts at:    493    1204    3774    6586    7458     493 
   Size:        711    2570    2812     872    2702

  Fragments arranged by size: 

                2812    2702    2570     872     711
 
BsaBI GATnn'nnATC

Cuts at:   9661    9661 
   Size:       9667
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4362    5775    7474    7856 
   Size:         53      83     186     936    2829    1413    1699     382

Cuts at:   7856    8491     275 
   Size:        635    1451

  Fragments arranged by size: 

                2829    1699    1451    1413     936     635     382     186
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   5055    6447    8100    8498    8772    8919    9330    5055 
   Size:       1392    1653     398     274     147     411    5392

  Fragments arranged by size: 

                5392    1653    1392     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    4193    4223    4259    4289    4671 
   Size:         30     165      30    2790      30      36      30     382

Cuts at:   4671    4701    6747    6777    6845    6875    1178 
   Size:         30    2046      30      68      30    3970

  Fragments arranged by size: 

                3970    2790    2046     382     165      68      36      30
                  30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2327    2528    2561    2591    2747    3857    4283    5486 
   Size:       1141     201      33      30     156    1110     426    1203

Cuts at:   5486    5939    1186 
   Size:        453    4914

  Fragments arranged by size: 

                4914    1203    1141    1110     453     426     201     156
                  33      30
 
BseYI C'CCAG_C

Cuts at:   2502    3186    4157    5092    5737    6568    8870    9366    2502 
   Size:        684     971     935     645     831    2302     496    2803

  Fragments arranged by size: 

                2803    2302     971     935     831     684     645     496
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2852    2873    3337    4032    4280    4493    5158 
   Size:        573    1116      21     464     695     248     213     665

Cuts at:   5158    6410    1163 
   Size:       1252    4420

  Fragments arranged by size: 

                4420    1252    1116     695     665     573     464     248
                 213      21
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    7117    7878    8906    1540 
   Size:        275     186    5116     761    1028    2301

  Fragments arranged by size: 

                5116    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2601    3519    4635    4803    5301    6294    6585    7728    7813 
   Size:        918    1116     168     498     993     291    1143      85

Cuts at:   7813    8884    2601 
   Size:       1071    3384

  Fragments arranged by size: 

                3384    1143    1116    1071     993     918     498     291
                 168      85
 
BsmI GAATG_Cn'

Cuts at:   3097    5197    7178    7271    3097 
   Size:       2100    1981      93    5493

  Fragments arranged by size: 

                5493    2100    1981      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2447    2447 
   Size:       9667
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2312    2601    2672    2841    3467    3519    3724 
   Size:        399     522     289      71     169     626      52     205

Cuts at:   3724    3730    4158    4635    4803    4846    5301    6152    6294 
   Size:          6     428     477     168      43     455     851     142

Cuts at:   6294    6585    6986    7728    7813    8884    1391 
   Size:        291     401     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     851     742     626     522     477     455
                 428     401     399     291     289     205     169     168
                 142      85      71      52      43       6
 
BspEI T'CCGG_A

Cuts at:   6447    9330    6447 
   Size:       2883    6784
 
BspHI T'CATG_A

Cuts at:   2200    7556    9286    2200 
   Size:       5356    1730    2581

  Fragments arranged by size: 

                5356    2581    1730
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2232    2610    2895    3382    3634    4840    7022     844 
   Size:       1388     378     285     487     252    1206    2182    3489

  Fragments arranged by size: 

                3489    2182    1388    1206     487     378     285     252
            
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4285    6463    7554    1394 
   Size:        429    2462    2178    1091    3507

  Fragments arranged by size: 

                3507    2462    2178    1091     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4732    6864    8162    8336      65 
   Size:       1183    3484    2132    1298     174    1396

  Fragments arranged by size: 

                3484    2132    1396    1298    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3146    4883    4968    8311    8498    1700 
   Size:        110    1336    1737      85    3343     187    2869

  Fragments arranged by size: 

                3343    2869    1737    1336     187     110      85
 
BsrGI T'GTAC_A

Cuts at:     96    3637      96 
   Size:       3541    6126
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       9667
 
BssSI C'ACGA_G

Cuts at:   2234    4280    4802    7727    8739    2234 
   Size:       2046     522    2925    1012    3162

  Fragments arranged by size: 

                3162    2925    2046    1012     522
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2758    2775    2887    3315    3523    4951     794 
   Size:       1964      17     112     428     208    1428    5510

  Fragments arranged by size: 

                5510    1964    1428     428     208     112      17
 
BstBI TT'CG_AA

Cuts at:   8471    8471 
   Size:       9667
 
BstEII G'GTnAC_C

Cuts at:   1184    3394    5614    1184 
   Size:       2210    2220    5237

  Fragments arranged by size: 

                5237    2220    2210
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2857    3190    5920    6836    2857 
   Size:        333    2730     916    5688

  Fragments arranged by size: 

                5688    2730     916     333
 
BstYI r'GATC_y

Cuts at:   1972    2370    3177    3846    4504    4719    5412    5928    5940 
   Size:        398     807     669     658     215     693     516      12

Cuts at:   5940    5982    6483    6946    6997    7364    7749    7766    9218 
   Size:         42     501     463      51     367     385      17    1452

Cuts at:   9218    9304    9316    9662    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     807     693     669     658     516     501
                 463     398     385     367     346     215      86      51
                  42      17      12      12
 
Bsu36I CC'TnA_GG

Cuts at:   5651    6042    5651 
   Size:        391    9276
 
BtgI C'CryG_G

Cuts at:    513    1065    3123    3667    5595    5619     513 
   Size:        552    2058     544    1928      24    4561

  Fragments arranged by size: 

                4561    2058    1928     552     544      24
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    8928

  Fragments arranged by size: 

                8928     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2771    2821    3325    3519    4673    6055    7183    7977    7997 
   Size:         50     504     194    1154    1382    1128     794      20

Cuts at:   7997    2771 
   Size:       4441

  Fragments arranged by size: 

                4441    1382    1154    1128     794     504     194      50
                  20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       9667
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2472    2507    6946    6981     529 
   Size:         35    1908      35    4439      35    3215

  Fragments arranged by size: 

                4439    3215    1908      35      35      35
 
DraI TTT'AAA

Cuts at:   6747    6971    6747 
   Size:        224    9443
 
DraIII CAC_nnn'GTG

Cuts at:   3523    3523 
   Size:       9667
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    8674     809 
   Size:        507    7358    1802

  Fragments arranged by size: 

                7358    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2247    3489    4818 
   Size:         54    1425      50     275      18     417    1242    1329

Cuts at:   4818    4885    5559    6186    7114    8003    9632       8 
   Size:         67     674     627     928     889    1629      43

  Fragments arranged by size: 

                1629    1425    1329    1242     928     889     674     627
                 417     275      67      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    7114    1537 
   Size:        275    5302    4090

  Fragments arranged by size: 

                5302    4090     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2951    3200    3731    3750    5790    6205    7597    1353 
   Size:       1598     249     531      19    2040     415    1392    3423

  Fragments arranged by size: 

                3423    2040    1598    1392     531     415     249      19
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2181    5203    8254    8628    8774     387 
   Size:       1064     138     592    3022    3051     374     146    1280

  Fragments arranged by size: 

                3051    3022    1280    1064     592     374     146     138
            
 
EcoICRI GAG'CTC

Cuts at:   4633    5299    6292    4633 
   Size:        666     993    8008

  Fragments arranged by size: 

                8008     993     666
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2594    2609    2738    2990    3038    3587    3717 
   Size:        393     962      15     129     252      48     549     130

Cuts at:   3717    3890    3968    4673    4716    4862    4976    5054    5478 
   Size:        173      78     705      43     146     114      78     424

Cuts at:   5478    5699    5741    5831    6041    6158    6272    6326    6392 
   Size:        221      42      90     210     117     114      54      66

Cuts at:   6392    6504    6743    6963    7730    8327    9114    9382    1239 
   Size:        112     239     220     767     597     787     268    1524

  Fragments arranged by size: 

                1524     962     787     767     705     597     549     424
                 393     268     252     239     221     220     210     173
                 146     130     129     117     114     114     112      90
                  78      78      66      54      48      43      42      15
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3140    3986    3140 
   Size:        846    8821
 
EcoO109I rG'GnC_Cy

Cuts at:   2320    2476    2620    2722    3727    4411    5264    5716    6430 
   Size:        156     144     102    1005     684     853     452     714

Cuts at:   6430    6876    6938    2320 
   Size:        446      62    5049

  Fragments arranged by size: 

                5049    1005     853     714     684     452     446     156
                 144     102      62
 
EcoRI G'AATT_C

Cuts at:   5504    6976    7354    5504 
   Size:       1472     378    7817

  Fragments arranged by size: 

                7817    1472     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2152    2443    3455    4397    5176    2152 
   Size:        291    1012     942     779    6643

  Fragments arranged by size: 

                6643    1012     942     779     291
 
FseI GG_CCGG'CC

Cuts at:   4887    4887 
   Size:       9667
 
FspI TGC'GCA

Cuts at:   2909    3878    3896    4319    8173    2909 
   Size:        969      18     423    3854    4403

  Fragments arranged by size: 

                4403    3854     969     423      18
 
FspAI rTGC'GCAy

Cuts at:   3878    3878 
   Size:       9667
 
HaeII r_GCGC'y

Cuts at:   1678    2595    4139    4444    5394    5405    8814    1678 
   Size:        917    1544     305     950      11    3409    2531

  Fragments arranged by size: 

                3409    2531    1544     950     917     305      11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2057    2089    2774    2806    2836 
   Size:         32     135      32     485      32     685      32      30

Cuts at:   2836    2868    4057    4089    5299    5331    6422    6454    6845 
   Size:         32    1189      32    1210      32    1091      32     391

Cuts at:   6845    6877    6938    6970    8308    8340    8382    8414    1373 
   Size:         32      61      32    1338      32      42      32    2626

  Fragments arranged by size: 

                2626    1338    1210    1189    1091     685     485     391
                 135      61      42      32      32      32      32      32
                  32      32      32      32      32      32      32      30
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3103    5698    7011    7257     669 
   Size:        988     281    1165    2595    1313     246    3079

  Fragments arranged by size: 

                3079    2595    1313    1165     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2097    3914    5543     748 
   Size:       1349    1817    1629    4872

  Fragments arranged by size: 

                4872    1817    1629    1349
 
HpaI GTT'AAC

Cuts at:   7257    7257 
   Size:       9667
 
KpnI G_GTAC'C

Cuts at:   6992    6992 
   Size:       9667
 
MfeI C'AATT_G

Cuts at:   7266    7266 
   Size:       9667
 
MluI A'CGCG_T

Cuts at:   9543    9543 
   Size:       9667
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3630    3978    4209    4755    4837    6147    6690    8781 
   Size:       2325     348     231     546      82    1310     543    2091

Cuts at:   8781    8965    1305 
   Size:        184    2007

  Fragments arranged by size: 

                2325    2091    2007    1310     546     543     348     231
                 184      82
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2249    4820    6188      10 
   Size:         54    1425     343     417    2571    1368    3489

  Fragments arranged by size: 

                3489    2571    1425    1368     417     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2300    3122    4343    4839    4893    5589    7625 
   Size:        648    1134     822    1221     496      54     696    2036

Cuts at:   7625    7984    8143     518 
   Size:        359     159    2042

  Fragments arranged by size: 

                2042    2036    1221    1134     822     696     648     496
                 359     159      54
 
MspA1I CmG'CkG

Cuts at:   1606    2366    2506    2755    2775    2938    3190    3454    3607 
   Size:        760     140     249      20     163     252     264     153

Cuts at:   3607    3910    4432    4789    4795    5557    6175    7083    7760 
   Size:        303     522     357       6     762     618     908     677

Cuts at:   7760    8908    9153    1606 
   Size:       1148     245    2120

  Fragments arranged by size: 

                2120    1148     908     762     760     677     618     522
                 357     303     264     252     249     245     163     153
                 140      20       6
 
NaeI GCC'GGC

Cuts at:   4885    4885 
   Size:       9667
 
NcoI C'CATG_G

Cuts at:    513    1065    3667     513 
   Size:        552    2602    6513

  Fragments arranged by size: 

                6513    2602     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       9667
 
NgoMIV G'CCGG_C

Cuts at:   4883    4883 
   Size:       9667
 
NheI G'CTAG_C

Cuts at:   1052    2635    4459    1052 
   Size:       1583    1824    6260

  Fragments arranged by size: 

                6260    1824    1583
 
NotI GC'GGCC_GC

Cuts at:   7114    7114 
   Size:       9667
 
NsiI A_TGCA'T

Cuts at:   6351    6351 
   Size:       9667
 
NspI r_CATG'y

Cuts at:   1132    1229    2305    2906    3931    5239    6146    6353    6361 
   Size:         97    1076     601    1025    1308     907     207       8

Cuts at:   6361    7025    8570    1132 
   Size:        664    1545    2229

  Fragments arranged by size: 

                2229    1545    1308    1076    1025     907     664     601
                 207      97       8
 
PasI CC'CwG_GG

Cuts at:   1348    3467    5936    1348 
   Size:       2119    2469    5079

  Fragments arranged by size: 

                5079    2469    2119
 
PciI A'CATG_T

Cuts at:   3927    8566    3927 
   Size:       4639    5028
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2833    3526    1108 
   Size:       1725     693    7249

  Fragments arranged by size: 

                7249    1725     693
 
PfoI T'CCnGG_A

Cuts at:   2625    2856    3921    4032    4908    5031    6135    6408    2625 
   Size:        231    1065     111     876     123    1104     273    5884

  Fragments arranged by size: 

                5884    1104    1065     876     273     231     123     111
            
 
PmeI GTTT'AAAC

Cuts at:   6971    6971 
   Size:       9667
 
PmlI CAC'GTG

Cuts at:   6586    6586 
   Size:       9667
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2550    2582    4259    4291    7736    7768    9249    9281    2550 
   Size:         32    1677      32    3445      32    1481      32    2936

  Fragments arranged by size: 

                3445    2936    1677    1481      32      32      32      32
            
 
PpuMI rG'GwC_Cy

Cuts at:   2320    2620    4411    5264    5716    6430    6876    6938    2320 
   Size:        300    1791     853     452     714     446      62    5049

  Fragments arranged by size: 

                5049    1791     853     714     452     446     300      62
            
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       9667
 
PsiI TTA'TAA

Cuts at:   7237    7237 
   Size:       9667
 
PspOMI G'GGCC_C

Cuts at:   3726    3726 
   Size:       9667
 
PspXI vC'TCGA_Gb

Cuts at:   2615    2615 
   Size:       9667
 
PstI C_TGCA'G

Cuts at:    830    2352    2766    2780    3453    3615    3735    4239    4440 
   Size:       1522     414      14     673     162     120     504     201

Cuts at:   4440    4794    4851    5307    7019     830 
   Size:        354      57     456    1712    3478

  Fragments arranged by size: 

                3478    1712    1522     673     504     456     414     354
                 201     162     120      57      14
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       9667
 
PvuII CAG'CTG

Cuts at:   2366    2506    2938    3190    4789    5557    2366 
   Size:        140     432     252    1599     768    6476

  Fragments arranged by size: 

                6476    1599     768     432     252     140
 
RsrII CG'GwC_CG

Cuts at:   2011    2011 
   Size:       9667
 
SacI G_AGCT'C

Cuts at:   4635    5301    6294    4635 
   Size:        666     993    8008

  Fragments arranged by size: 

                8008     993     666
 
SalI G'TCGA_C

Cuts at:   1936    7009    1936 
   Size:       5073    4594
 
SanDI GG'GwC_CC

Cuts at:   5264    6430    5264 
   Size:       1166    8501
 
SapI GCTCTTCn'nnn_

Cuts at:   2951    3200    2951 
   Size:        249    9418
 
SbfI CC_TGCA'GG

Cuts at:   4851    7019    4851 
   Size:       2168    7499
 
ScaI AGT'ACT

Cuts at:   7915    9627    7915 
   Size:       1712    7955
 
SexAI A'CCwGG_T

Cuts at:   3519    3519 
   Size:       9667
 
SfcI C'TryA_G

Cuts at:    826    1045    2348    2762    2776    3449    3611    3731    4235 
   Size:        219    1303     414      14     673     162     120     504

Cuts at:   4235    4436    4790    4847    5303    5635    7015    8150    8831 
   Size:        201     354      57     456     332    1380    1135     681

Cuts at:   8831    9022     826 
   Size:        191    1471

  Fragments arranged by size: 

                1471    1380    1303    1135     681     673     504     456
                 414     354     332     219     201     191     162     120
                  57      14
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       9667
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       9667
 
SmaI CCC'GGG

Cuts at:   1872    2811    3725    6994    1872 
   Size:        939     914    3269    4545

  Fragments arranged by size: 

                4545    3269     939     914
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2615    2702    2915    5165    5207    6293 
   Size:        197     926     672      87     213    2250      42    1086

Cuts at:   6293    7771    8672    8934     820 
   Size:       1478     901     262    1553

  Fragments arranged by size: 

                2250    1553    1478    1086     926     901     672     262
                 213     197      87      42
 
SnaBI TAC'GTA

Cuts at:    493    3774    7458     493 
   Size:       3281    3684    2702

  Fragments arranged by size: 

                3684    3281    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       9667
 
SphI G_CATG'C

Cuts at:   1132    5239    6146    6361    7025    1132 
   Size:       4107     907     215     664    3774

  Fragments arranged by size: 

                4107    3774     907     664     215
 
SrfI GCCC'GGGC

Cuts at:   3725    3725 
   Size:       9667
 
SspI AAT'ATT

Cuts at:      5      52    7591       5 
   Size:         47    7539    2081

  Fragments arranged by size: 

                7539    2081      47
 
StuI AGG'CCT

Cuts at:   1517    3677    5159    5171    1517 
   Size:       2160    1482      12    6013

  Fragments arranged by size: 

                6013    2160    1482      12
 
StyI C'CwwG_G

Cuts at:    513    1065    3507    3667    5689    6489    7106     513 
   Size:        552    2442     160    2022     800     617    3074

  Fragments arranged by size: 

                3074    2442    2022     800     617     552     160
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3200    4219    5602    6560    6593    7694    7711 
   Size:       1255    1620    1019    1383     958      33    1101      17

Cuts at:   7711    7864    8049    8494     325 
   Size:        153     185     445    1498

  Fragments arranged by size: 

                1620    1498    1383    1255    1101    1019     958     445
                 185     153      33      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3637    4332    4515 
   Size:        274      80      33      51    1443    1660     695     183

Cuts at:   4515    5211    7913    9625      96 
   Size:        696    2702    1712     138

  Fragments arranged by size: 

                2702    1712    1660    1443     696     695     274     183
                 138      80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3185    3217    3290    3322    4193    4225    6747    6779    9627 
   Size:         32      73      32     871      32    2522      32    2848

Cuts at:   9627    9659    3185 
   Size:         32    3193

  Fragments arranged by size: 

                3193    2848    2522     871      73      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    5117    1316 
   Size:       3801    5866
 
XbaI T'CTAG_A

Cuts at:   4075    7003    4075 
   Size:       2928    6739
 
XhoI C'TCGA_G

Cuts at:   1943    2615    1943 
   Size:        672    8995
 
XmaI C'CCGG_G

Cuts at:   1870    2809    3723    6992    1870 
   Size:        939     914    3269    4545

  Fragments arranged by size: 

                4545    3269     939     914
 
XmnI GAAnn'nnTTC

Cuts at:   2358    3981    4397    6711    7796    2358 
   Size:       1623     416    2314    1085    4229

  Fragments arranged by size: 

                4229    2314    1623    1085     416
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    7475     276 
   Size:         53      83     186     936    5941    2468

  Fragments arranged by size: 

                5941    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AscI     BaeI     BbeI     BplI    BsiWI  BstZ17I    EcoRV     KasI
     NarI     NruI     PacI     PsrI    SacII     SfiI     SfoI     SwaI
     XcmI
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