(Circular) (Six-base) MAPSORT of: pFN21AB0009.seq Check: 2015 from: 1 to: 9667 pFN21AB0009 9667 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2610 2895 3382 3634 2610 Size: 285 487 252 8643 Fragments arranged by size: 8643 487 285 252 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7477 278 Size: 53 83 186 936 5941 2468 Fragments arranged by size: 5941 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7010 1937 Size: 5073 4594 Acc65I G'GTAC_C Cuts at: 6988 6988 Size: 9667 AclI AA'CG_TT Cuts at: 1563 7794 8167 1563 Size: 6231 373 3063 Fragments arranged by size: 6231 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2738 3968 4716 4976 6272 6504 6743 7730 Size: 1106 1230 748 260 1296 232 239 987 Cuts at: 7730 9114 9382 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1296 1230 1106 987 748 268 260 239 232 AfeI AGC'GCT Cuts at: 5392 5392 Size: 9667 AflII C'TTAA_G Cuts at: 820 1017 5165 820 Size: 197 4148 5322 Fragments arranged by size: 5322 4148 197 AflIII A'CryG_T Cuts at: 1203 2268 3927 8566 9543 1203 Size: 1065 1659 4639 977 1327 Fragments arranged by size: 4639 1659 1327 1065 977 AgeI A'CCGG_T Cuts at: 8498 8498 Size: 9667 AhdI GACnn_n'nnGTC Cuts at: 6436 8396 6436 Size: 1960 7707 AleI CACnn'nnGTG Cuts at: 1166 4839 1166 Size: 3673 5994 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4857 1184 Size: 3673 5994 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3464 3496 4259 4291 3464 Size: 32 763 32 8840 Fragments arranged by size: 8840 763 32 32 AlwNI CAG_nnn'CTG Cuts at: 2758 2833 2887 3286 3526 3610 4019 4039 4435 Size: 75 54 399 240 84 409 20 396 Cuts at: 4435 4507 4711 5485 7410 8982 2758 Size: 72 204 774 1925 1572 3443 Fragments arranged by size: 3443 1925 1572 774 409 399 396 240 204 84 75 72 54 20 ApaI G_GGCC'C Cuts at: 3730 3730 Size: 9667 ApaLI G'TGCA_C Cuts at: 2597 3515 7724 8880 2597 Size: 918 4209 1156 3384 Fragments arranged by size: 4209 3384 1156 918 ApoI r'AATT_y Cuts at: 1493 2441 4601 5429 5504 5825 6080 6605 6976 Size: 948 2160 828 75 321 255 525 371 Cuts at: 6976 7202 7354 9463 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2160 2109 1697 948 828 525 371 321 255 226 152 75 AseI AT'TA_AT Cuts at: 160 1032 8221 160 Size: 872 7189 1606 Fragments arranged by size: 7189 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9667 AvaI C'yCGr_G Cuts at: 1870 1943 2615 2809 3723 3839 4148 5259 5267 Size: 73 672 194 914 116 309 1111 8 Cuts at: 5267 6992 1870 Size: 1725 4545 Fragments arranged by size: 4545 1725 1111 914 672 309 194 116 73 8 AvrII C'CTAG_G Cuts at: 5689 5689 Size: 9667 BamHI G'GATC_C Cuts at: 4719 5412 5940 6997 7364 4719 Size: 693 528 1057 367 7022 Fragments arranged by size: 7022 1057 693 528 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2836 5512 6149 6930 6988 Size: 325 217 627 1049 2676 637 781 58 Cuts at: 6988 8443 618 Size: 1455 1842 Fragments arranged by size: 2676 1842 1455 1049 781 637 627 325 217 58 BanII G_rGCy'C Cuts at: 1391 2312 2672 3467 3724 3730 4158 4635 5301 Size: 921 360 795 257 6 428 477 666 Cuts at: 5301 6294 6986 1391 Size: 993 692 4072 Fragments arranged by size: 4072 993 921 795 692 666 477 428 360 257 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2226 2426 3616 3984 4116 4380 4835 Size: 971 327 200 1190 368 132 264 455 Cuts at: 4835 4971 928 Size: 136 5624 Fragments arranged by size: 5624 1190 971 455 368 327 264 200 136 132 BbvCI CC'TCA_GC Cuts at: 5196 5196 Size: 9667 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2939 2973 3226 3260 4883 4917 5730 Size: 34 1002 34 253 34 1623 34 813 Cuts at: 5730 5764 7858 7892 1903 Size: 34 2094 34 3678 Fragments arranged by size: 3678 2094 1623 1002 813 253 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7559 8769 7559 Size: 1210 8457 BclI T'GATC_A Cuts at: 1548 4041 1548 Size: 2493 7174 BfrBI ATG'CAT Cuts at: 6349 6349 Size: 9667 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3426 4994 8278 Size: 107 122 71 631 605 1754 1568 3284 Cuts at: 8278 136 Size: 1525 Fragments arranged by size: 3284 1754 1568 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3177 3846 5982 6483 9662 3177 Size: 669 2136 501 3179 3182 Fragments arranged by size: 3182 3179 2136 669 501 BlpI GC'TnA_GC Cuts at: 3276 5394 7084 3276 Size: 2118 1690 5859 Fragments arranged by size: 5859 2118 1690 Bme1580I G_kGCm'C Cuts at: 1790 2601 2841 3519 3730 4846 6152 7728 8884 Size: 811 240 678 211 1116 1306 1576 1156 Cuts at: 8884 1790 Size: 2573 Fragments arranged by size: 2573 1576 1306 1156 1116 811 678 240 211 BmgBI CAC'GTC Cuts at: 1318 2269 3532 1318 Size: 951 1263 7453 Fragments arranged by size: 7453 1263 951 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5235 7398 8356 Size: 416 53 83 399 24 3818 2163 958 Cuts at: 8356 442 Size: 1753 Fragments arranged by size: 3818 2163 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2639 4463 1056 Size: 1583 1824 6260 Fragments arranged by size: 6260 1824 1583 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2594 2609 2990 3038 3587 3717 3890 4673 Size: 1355 15 381 48 549 130 173 783 Cuts at: 4673 4862 5054 5478 5699 5741 5831 6041 6158 Size: 189 192 424 221 42 90 210 117 Cuts at: 6158 6326 6392 6963 8327 1239 Size: 168 66 571 1364 2579 Fragments arranged by size: 2579 1364 1355 783 571 549 424 381 221 210 192 189 173 168 130 117 90 66 48 42 15 Bpu10I CC'TnA_GC Cuts at: 3461 4127 5196 3461 Size: 666 1069 7932 Fragments arranged by size: 7932 1069 666 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2687 2900 5192 6278 7792 8657 8955 2687 Size: 213 2292 1086 1514 865 298 3399 Fragments arranged by size: 3399 2292 1514 1086 865 298 213 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5456 5885 8330 882 Size: 617 3957 429 2445 2219 Fragments arranged by size: 3957 2445 2219 617 429 BsaAI yAC'GTr Cuts at: 493 1204 3774 6586 7458 493 Size: 711 2570 2812 872 2702 Fragments arranged by size: 2812 2702 2570 872 711 BsaBI GATnn'nnATC Cuts at: 9661 9661 Size: 9667 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4362 5775 7474 7856 Size: 53 83 186 936 2829 1413 1699 382 Cuts at: 7856 8491 275 Size: 635 1451 Fragments arranged by size: 2829 1699 1451 1413 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5055 6447 8100 8498 8772 8919 9330 5055 Size: 1392 1653 398 274 147 411 5392 Fragments arranged by size: 5392 1653 1392 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4193 4223 4259 4289 4671 Size: 30 165 30 2790 30 36 30 382 Cuts at: 4671 4701 6747 6777 6845 6875 1178 Size: 30 2046 30 68 30 3970 Fragments arranged by size: 3970 2790 2046 382 165 68 36 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2327 2528 2561 2591 2747 3857 4283 5486 Size: 1141 201 33 30 156 1110 426 1203 Cuts at: 5486 5939 1186 Size: 453 4914 Fragments arranged by size: 4914 1203 1141 1110 453 426 201 156 33 30 BseYI C'CCAG_C Cuts at: 2502 3186 4157 5092 5737 6568 8870 9366 2502 Size: 684 971 935 645 831 2302 496 2803 Fragments arranged by size: 2803 2302 971 935 831 684 645 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2852 2873 3337 4032 4280 4493 5158 Size: 573 1116 21 464 695 248 213 665 Cuts at: 5158 6410 1163 Size: 1252 4420 Fragments arranged by size: 4420 1252 1116 695 665 573 464 248 213 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7117 7878 8906 1540 Size: 275 186 5116 761 1028 2301 Fragments arranged by size: 5116 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2601 3519 4635 4803 5301 6294 6585 7728 7813 Size: 918 1116 168 498 993 291 1143 85 Cuts at: 7813 8884 2601 Size: 1071 3384 Fragments arranged by size: 3384 1143 1116 1071 993 918 498 291 168 85 BsmI GAATG_Cn' Cuts at: 3097 5197 7178 7271 3097 Size: 2100 1981 93 5493 Fragments arranged by size: 5493 2100 1981 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2447 2447 Size: 9667 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2312 2601 2672 2841 3467 3519 3724 Size: 399 522 289 71 169 626 52 205 Cuts at: 3724 3730 4158 4635 4803 4846 5301 6152 6294 Size: 6 428 477 168 43 455 851 142 Cuts at: 6294 6585 6986 7728 7813 8884 1391 Size: 291 401 742 85 1071 2174 Fragments arranged by size: 2174 1071 851 742 626 522 477 455 428 401 399 291 289 205 169 168 142 85 71 52 43 6 BspEI T'CCGG_A Cuts at: 6447 9330 6447 Size: 2883 6784 BspHI T'CATG_A Cuts at: 2200 7556 9286 2200 Size: 5356 1730 2581 Fragments arranged by size: 5356 2581 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2232 2610 2895 3382 3634 4840 7022 844 Size: 1388 378 285 487 252 1206 2182 3489 Fragments arranged by size: 3489 2182 1388 1206 487 378 285 252 BsrBI CCG'CTC Cuts at: 1394 1823 4285 6463 7554 1394 Size: 429 2462 2178 1091 3507 Fragments arranged by size: 3507 2462 2178 1091 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4732 6864 8162 8336 65 Size: 1183 3484 2132 1298 174 1396 Fragments arranged by size: 3484 2132 1396 1298 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3146 4883 4968 8311 8498 1700 Size: 110 1336 1737 85 3343 187 2869 Fragments arranged by size: 3343 2869 1737 1336 187 110 85 BsrGI T'GTAC_A Cuts at: 96 3637 96 Size: 3541 6126 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9667 BssSI C'ACGA_G Cuts at: 2234 4280 4802 7727 8739 2234 Size: 2046 522 2925 1012 3162 Fragments arranged by size: 3162 2925 2046 1012 522 BstAPI GCAn_nnn'nTGC Cuts at: 794 2758 2775 2887 3315 3523 4951 794 Size: 1964 17 112 428 208 1428 5510 Fragments arranged by size: 5510 1964 1428 428 208 112 17 BstBI TT'CG_AA Cuts at: 8471 8471 Size: 9667 BstEII G'GTnAC_C Cuts at: 1184 3394 5614 1184 Size: 2210 2220 5237 Fragments arranged by size: 5237 2220 2210 BstXI CCAn_nnnn'nTGG Cuts at: 2857 3190 5920 6836 2857 Size: 333 2730 916 5688 Fragments arranged by size: 5688 2730 916 333 BstYI r'GATC_y Cuts at: 1972 2370 3177 3846 4504 4719 5412 5928 5940 Size: 398 807 669 658 215 693 516 12 Cuts at: 5940 5982 6483 6946 6997 7364 7749 7766 9218 Size: 42 501 463 51 367 385 17 1452 Cuts at: 9218 9304 9316 9662 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 807 693 669 658 516 501 463 398 385 367 346 215 86 51 42 17 12 12 Bsu36I CC'TnA_GG Cuts at: 5651 6042 5651 Size: 391 9276 BtgI C'CryG_G Cuts at: 513 1065 3123 3667 5595 5619 513 Size: 552 2058 544 1928 24 4561 Fragments arranged by size: 4561 2058 1928 552 544 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8928 Fragments arranged by size: 8928 683 56 BtsI GCAGTG_nn' Cuts at: 2771 2821 3325 3519 4673 6055 7183 7977 7997 Size: 50 504 194 1154 1382 1128 794 20 Cuts at: 7997 2771 Size: 4441 Fragments arranged by size: 4441 1382 1154 1128 794 504 194 50 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9667 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2472 2507 6946 6981 529 Size: 35 1908 35 4439 35 3215 Fragments arranged by size: 4439 3215 1908 35 35 35 DraI TTT'AAA Cuts at: 6747 6971 6747 Size: 224 9443 DraIII CAC_nnn'GTG Cuts at: 3523 3523 Size: 9667 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8674 809 Size: 507 7358 1802 Fragments arranged by size: 7358 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2247 3489 4818 Size: 54 1425 50 275 18 417 1242 1329 Cuts at: 4818 4885 5559 6186 7114 8003 9632 8 Size: 67 674 627 928 889 1629 43 Fragments arranged by size: 1629 1425 1329 1242 928 889 674 627 417 275 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7114 1537 Size: 275 5302 4090 Fragments arranged by size: 5302 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2951 3200 3731 3750 5790 6205 7597 1353 Size: 1598 249 531 19 2040 415 1392 3423 Fragments arranged by size: 3423 2040 1598 1392 531 415 249 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2181 5203 8254 8628 8774 387 Size: 1064 138 592 3022 3051 374 146 1280 Fragments arranged by size: 3051 3022 1280 1064 592 374 146 138 EcoICRI GAG'CTC Cuts at: 4633 5299 6292 4633 Size: 666 993 8008 Fragments arranged by size: 8008 993 666 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2594 2609 2738 2990 3038 3587 3717 Size: 393 962 15 129 252 48 549 130 Cuts at: 3717 3890 3968 4673 4716 4862 4976 5054 5478 Size: 173 78 705 43 146 114 78 424 Cuts at: 5478 5699 5741 5831 6041 6158 6272 6326 6392 Size: 221 42 90 210 117 114 54 66 Cuts at: 6392 6504 6743 6963 7730 8327 9114 9382 1239 Size: 112 239 220 767 597 787 268 1524 Fragments arranged by size: 1524 962 787 767 705 597 549 424 393 268 252 239 221 220 210 173 146 130 129 117 114 114 112 90 78 78 66 54 48 43 42 15 EcoNI CCTnn'n_nnAGG Cuts at: 3140 3986 3140 Size: 846 8821 EcoO109I rG'GnC_Cy Cuts at: 2320 2476 2620 2722 3727 4411 5264 5716 6430 Size: 156 144 102 1005 684 853 452 714 Cuts at: 6430 6876 6938 2320 Size: 446 62 5049 Fragments arranged by size: 5049 1005 853 714 684 452 446 156 144 102 62 EcoRI G'AATT_C Cuts at: 5504 6976 7354 5504 Size: 1472 378 7817 Fragments arranged by size: 7817 1472 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2152 2443 3455 4397 5176 2152 Size: 291 1012 942 779 6643 Fragments arranged by size: 6643 1012 942 779 291 FseI GG_CCGG'CC Cuts at: 4887 4887 Size: 9667 FspI TGC'GCA Cuts at: 2909 3878 3896 4319 8173 2909 Size: 969 18 423 3854 4403 Fragments arranged by size: 4403 3854 969 423 18 FspAI rTGC'GCAy Cuts at: 3878 3878 Size: 9667 HaeII r_GCGC'y Cuts at: 1678 2595 4139 4444 5394 5405 8814 1678 Size: 917 1544 305 950 11 3409 2531 Fragments arranged by size: 3409 2531 1544 950 917 305 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2057 2089 2774 2806 2836 Size: 32 135 32 485 32 685 32 30 Cuts at: 2836 2868 4057 4089 5299 5331 6422 6454 6845 Size: 32 1189 32 1210 32 1091 32 391 Cuts at: 6845 6877 6938 6970 8308 8340 8382 8414 1373 Size: 32 61 32 1338 32 42 32 2626 Fragments arranged by size: 2626 1338 1210 1189 1091 685 485 391 135 61 42 32 32 32 32 32 32 32 32 32 32 32 32 30 HincII GTy'rAC Cuts at: 669 1657 1938 3103 5698 7011 7257 669 Size: 988 281 1165 2595 1313 246 3079 Fragments arranged by size: 3079 2595 1313 1165 988 281 246 HindIII A'AGCT_T Cuts at: 748 2097 3914 5543 748 Size: 1349 1817 1629 4872 Fragments arranged by size: 4872 1817 1629 1349 HpaI GTT'AAC Cuts at: 7257 7257 Size: 9667 KpnI G_GTAC'C Cuts at: 6992 6992 Size: 9667 MfeI C'AATT_G Cuts at: 7266 7266 Size: 9667 MluI A'CGCG_T Cuts at: 9543 9543 Size: 9667 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3630 3978 4209 4755 4837 6147 6690 8781 Size: 2325 348 231 546 82 1310 543 2091 Cuts at: 8781 8965 1305 Size: 184 2007 Fragments arranged by size: 2325 2091 2007 1310 546 543 348 231 184 82 MscI TGG'CCA Cuts at: 10 64 1489 1832 2249 4820 6188 10 Size: 54 1425 343 417 2571 1368 3489 Fragments arranged by size: 3489 2571 1425 1368 417 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2300 3122 4343 4839 4893 5589 7625 Size: 648 1134 822 1221 496 54 696 2036 Cuts at: 7625 7984 8143 518 Size: 359 159 2042 Fragments arranged by size: 2042 2036 1221 1134 822 696 648 496 359 159 54 MspA1I CmG'CkG Cuts at: 1606 2366 2506 2755 2775 2938 3190 3454 3607 Size: 760 140 249 20 163 252 264 153 Cuts at: 3607 3910 4432 4789 4795 5557 6175 7083 7760 Size: 303 522 357 6 762 618 908 677 Cuts at: 7760 8908 9153 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 908 762 760 677 618 522 357 303 264 252 249 245 163 153 140 20 6 NaeI GCC'GGC Cuts at: 4885 4885 Size: 9667 NcoI C'CATG_G Cuts at: 513 1065 3667 513 Size: 552 2602 6513 Fragments arranged by size: 6513 2602 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9667 NgoMIV G'CCGG_C Cuts at: 4883 4883 Size: 9667 NheI G'CTAG_C Cuts at: 1052 2635 4459 1052 Size: 1583 1824 6260 Fragments arranged by size: 6260 1824 1583 NotI GC'GGCC_GC Cuts at: 7114 7114 Size: 9667 NsiI A_TGCA'T Cuts at: 6351 6351 Size: 9667 NspI r_CATG'y Cuts at: 1132 1229 2305 2906 3931 5239 6146 6353 6361 Size: 97 1076 601 1025 1308 907 207 8 Cuts at: 6361 7025 8570 1132 Size: 664 1545 2229 Fragments arranged by size: 2229 1545 1308 1076 1025 907 664 601 207 97 8 PasI CC'CwG_GG Cuts at: 1348 3467 5936 1348 Size: 2119 2469 5079 Fragments arranged by size: 5079 2469 2119 PciI A'CATG_T Cuts at: 3927 8566 3927 Size: 4639 5028 PflMI CCAn_nnn'nTGG Cuts at: 1108 2833 3526 1108 Size: 1725 693 7249 Fragments arranged by size: 7249 1725 693 PfoI T'CCnGG_A Cuts at: 2625 2856 3921 4032 4908 5031 6135 6408 2625 Size: 231 1065 111 876 123 1104 273 5884 Fragments arranged by size: 5884 1104 1065 876 273 231 123 111 PmeI GTTT'AAAC Cuts at: 6971 6971 Size: 9667 PmlI CAC'GTG Cuts at: 6586 6586 Size: 9667 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2550 2582 4259 4291 7736 7768 9249 9281 2550 Size: 32 1677 32 3445 32 1481 32 2936 Fragments arranged by size: 3445 2936 1677 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2320 2620 4411 5264 5716 6430 6876 6938 2320 Size: 300 1791 853 452 714 446 62 5049 Fragments arranged by size: 5049 1791 853 714 452 446 300 62 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9667 PsiI TTA'TAA Cuts at: 7237 7237 Size: 9667 PspOMI G'GGCC_C Cuts at: 3726 3726 Size: 9667 PspXI vC'TCGA_Gb Cuts at: 2615 2615 Size: 9667 PstI C_TGCA'G Cuts at: 830 2352 2766 2780 3453 3615 3735 4239 4440 Size: 1522 414 14 673 162 120 504 201 Cuts at: 4440 4794 4851 5307 7019 830 Size: 354 57 456 1712 3478 Fragments arranged by size: 3478 1712 1522 673 504 456 414 354 201 162 120 57 14 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9667 PvuII CAG'CTG Cuts at: 2366 2506 2938 3190 4789 5557 2366 Size: 140 432 252 1599 768 6476 Fragments arranged by size: 6476 1599 768 432 252 140 RsrII CG'GwC_CG Cuts at: 2011 2011 Size: 9667 SacI G_AGCT'C Cuts at: 4635 5301 6294 4635 Size: 666 993 8008 Fragments arranged by size: 8008 993 666 SalI G'TCGA_C Cuts at: 1936 7009 1936 Size: 5073 4594 SanDI GG'GwC_CC Cuts at: 5264 6430 5264 Size: 1166 8501 SapI GCTCTTCn'nnn_ Cuts at: 2951 3200 2951 Size: 249 9418 SbfI CC_TGCA'GG Cuts at: 4851 7019 4851 Size: 2168 7499 ScaI AGT'ACT Cuts at: 7915 9627 7915 Size: 1712 7955 SexAI A'CCwGG_T Cuts at: 3519 3519 Size: 9667 SfcI C'TryA_G Cuts at: 826 1045 2348 2762 2776 3449 3611 3731 4235 Size: 219 1303 414 14 673 162 120 504 Cuts at: 4235 4436 4790 4847 5303 5635 7015 8150 8831 Size: 201 354 57 456 332 1380 1135 681 Cuts at: 8831 9022 826 Size: 191 1471 Fragments arranged by size: 1471 1380 1303 1135 681 673 504 456 414 354 332 219 201 191 162 120 57 14 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9667 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9667 SmaI CCC'GGG Cuts at: 1872 2811 3725 6994 1872 Size: 939 914 3269 4545 Fragments arranged by size: 4545 3269 939 914 SmlI C'TyrA_G Cuts at: 820 1017 1943 2615 2702 2915 5165 5207 6293 Size: 197 926 672 87 213 2250 42 1086 Cuts at: 6293 7771 8672 8934 820 Size: 1478 901 262 1553 Fragments arranged by size: 2250 1553 1478 1086 926 901 672 262 213 197 87 42 SnaBI TAC'GTA Cuts at: 493 3774 7458 493 Size: 3281 3684 2702 Fragments arranged by size: 3684 3281 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9667 SphI G_CATG'C Cuts at: 1132 5239 6146 6361 7025 1132 Size: 4107 907 215 664 3774 Fragments arranged by size: 4107 3774 907 664 215 SrfI GCCC'GGGC Cuts at: 3725 3725 Size: 9667 SspI AAT'ATT Cuts at: 5 52 7591 5 Size: 47 7539 2081 Fragments arranged by size: 7539 2081 47 StuI AGG'CCT Cuts at: 1517 3677 5159 5171 1517 Size: 2160 1482 12 6013 Fragments arranged by size: 6013 2160 1482 12 StyI C'CwwG_G Cuts at: 513 1065 3507 3667 5689 6489 7106 513 Size: 552 2442 160 2022 800 617 3074 Fragments arranged by size: 3074 2442 2022 800 617 552 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3200 4219 5602 6560 6593 7694 7711 Size: 1255 1620 1019 1383 958 33 1101 17 Cuts at: 7711 7864 8049 8494 325 Size: 153 185 445 1498 Fragments arranged by size: 1620 1498 1383 1255 1101 1019 958 445 185 153 33 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3637 4332 4515 Size: 274 80 33 51 1443 1660 695 183 Cuts at: 4515 5211 7913 9625 96 Size: 696 2702 1712 138 Fragments arranged by size: 2702 1712 1660 1443 696 695 274 183 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3185 3217 3290 3322 4193 4225 6747 6779 9627 Size: 32 73 32 871 32 2522 32 2848 Cuts at: 9627 9659 3185 Size: 32 3193 Fragments arranged by size: 3193 2848 2522 871 73 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5117 1316 Size: 3801 5866 XbaI T'CTAG_A Cuts at: 4075 7003 4075 Size: 2928 6739 XhoI C'TCGA_G Cuts at: 1943 2615 1943 Size: 672 8995 XmaI C'CCGG_G Cuts at: 1870 2809 3723 6992 1870 Size: 939 914 3269 4545 Fragments arranged by size: 4545 3269 939 914 XmnI GAAnn'nnTTC Cuts at: 2358 3981 4397 6711 7796 2358 Size: 1623 416 2314 1085 4229 Fragments arranged by size: 4229 2314 1623 1085 416 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7475 276 Size: 53 83 186 936 5941 2468 Fragments arranged by size: 5941 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BbeI BplI BsiWI BstZ17I EcoRV KasI NarI NruI PacI PsrI SacII SfiI SfoI SwaI XcmI