Restriction map for
pFN21AA1893
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BbeI BbrPI BbvCI BfrI BglI
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssSI BstBI BstEII BstPI
ClaI CpoI DraI DraIII EarI
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KasI KpnI Ksp632I MamI
MfeI MluI MroI MseI MslI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccI 675 GTATAC GT^[AC][GT]AC
AcyI 1154 GGCGTC G[GA]^CG[CT]C
AfaI 2344 GTAC GT^AC
ApaI 874 GGGCCC GGGCC^C
BalI 1167 TGGCCA TGG^CCA
BclI 2381 TGATCA T^GATCA
BsaHI 1154 GGCGTC G[GA]^CG[CT]C
BsrFI 2286 GCCGGC [GA]^CCGG[CT]
BssHII 2265 GCGCGC G^CGCGC
Bst1107I 675 GTATAC GTA^TAC
BstZ17I 675 GTATAC GTA^TAC
Cfr10I 2286 GCCGGC [GA]^CCGG[CT]
DrdI 2151 GACCAAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2381 TGATCA T^GATCA
HaeII 1939 GGCGCT [GA]GCGC^[CT]
Hin1I 1154 GGCGTC G[GA]^CG[CT]C
KspI 2291 CCGCGG CCGC^GG
MluNI 1167 TGGCCA TGG^CCA
MscI 1167 TGGCCA TGG^CCA
NaeI 2286 GCCGGC GCC^GGC
NgoMIV 2286 GCCGGC G^CCGGC
NspI 1042 ACATGC [GA]CATG^[CT]
PflMI 88 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 31 CAGCTG CAG^CTG
RsaI 2344 GTAC GT^AC
SacI 1297 GAGCTC GAGCT^C
SacII 2291 CCGCGG CCGC^GG
StuI 1994 AGGCCT AGG^CCT
Van91I 88 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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BanI 1752 GGTGCC G^G[CT][GA]CC
2218 GGTGCC
BcgI 2323 CGAGTGATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2186 GCAAAGCCCTCG
BfaI 206 CTAG C^TAG
1831 CTAG
BlpI 1531 GCTGAGC GC^T[ACGT]AGC
2198 GCTCAGC
Bpu1102I 1531 GCTGAGC GC^T[ACGT]AGC
2198 GCTCAGC
BsaI 1725 GGTCTC GGTCTC(1/5)
2207 GGTCTC
BsgI 161 CTGCAC GTGCAG(16/14)
2087 CTGCAC
BsiEI 2288 CGGCCG CG[GA][CT]^CG
2319 CGGCCG
BsrI 221 CCAGT ACTGG(1/-1)
1252 CCAGT
BstAPI 154 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2275 GCAGAGTGTGC
BstXI 1040 CCACATGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1490 CCAGGTCCTTGG
CelII 1531 GCTGAGC GC^T[ACGT]AGC
2198 GCTCAGC
DsaI 2291 CCGCGG C^C[GA][CT]GG
2316 CCACGG
EagI 2288 CGGCCG C^GGCCG
2319 CGGCCG
EclXI 2288 CGGCCG C^GGCCG
2319 CGGCCG
Eco52I 2288 CGGCCG C^GGCCG
2319 CGGCCG
HphI 310 TCACC GGTGA(8/7)
685 TCACC
MaeI 206 CTAG C^TAG
1831 CTAG
MaeII 373 ACGT A^CGT
673 ACGT
MspA1I 31 CAGCTG C[AC]G^C[GT]G
2291 CCGCGG
SfaNI 795 GCATC GCATC(5/9)
1066 GCATC
Tsp45I 309 GTCAC ^GT[GC]AC
684 GTCAC
XbaI 205 TCTAGA T^CTAGA
1830 TCTAGA
XcmI 260 CCAGAGGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
317 CCAAGGAGACATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AhdI AluI AlwI
AlwNI AocI AspEI AspHI AvaI
AvaII BamHI BanII BbsI BbvI
BciVI BcnI BglII BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI Eam1105I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI MaeIII MboI MboII MflI
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI Sau3AI Sau96I ScrFI
SfcI StyI TfiI TseI TspRI
XhoII