(Circular) (Six-base) MAPSORT of: pFN21AA1692.seq Check: 8858 from: 1 to: 7204 pFN21AA1692 7204 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3785 3785 Size: 7204 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5014 278 Size: 53 83 186 936 3478 2468 Fragments arranged by size: 3478 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4547 1937 Size: 2610 4594 Acc65I G'GTAC_C Cuts at: 4525 4525 Size: 7204 AclI AA'CG_TT Cuts at: 1563 5331 5704 1563 Size: 3768 373 3063 Fragments arranged by size: 3768 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2124 3507 3806 4057 5267 6651 6919 1632 Size: 492 1383 299 251 1210 1384 268 1917 Fragments arranged by size: 1917 1384 1383 1210 492 299 268 251 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7007 AflIII A'CryG_T Cuts at: 1203 4062 6103 7080 1203 Size: 2859 2041 977 1327 Fragments arranged by size: 2859 2041 1327 977 AgeI A'CCGG_T Cuts at: 6035 6035 Size: 7204 AhdI GACnn_n'nnGTC Cuts at: 5933 5933 Size: 7204 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7204 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4114 1184 Size: 2930 4274 AlwNI CAG_nnn'CTG Cuts at: 2925 3497 3575 3719 3883 4947 6519 2925 Size: 572 78 144 164 1064 1572 3610 Fragments arranged by size: 3610 1572 1064 572 164 144 78 ApaI G_GGCC'C Cuts at: 3035 3035 Size: 7204 ApaLI G'TGCA_C Cuts at: 5261 6417 5261 Size: 1156 6048 ApoI r'AATT_y Cuts at: 1493 2408 3155 3562 4513 4739 4891 7000 1493 Size: 915 747 407 951 226 152 2109 1697 Fragments arranged by size: 2109 1697 951 915 747 407 226 152 AseI AT'TA_AT Cuts at: 160 1032 2592 5758 160 Size: 872 1560 3166 1606 Fragments arranged by size: 3166 1606 1560 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7204 AvaI C'yCGr_G Cuts at: 1870 1943 2696 4359 4529 1870 Size: 73 753 1663 170 4545 Fragments arranged by size: 4545 1663 753 170 73 BamHI G'GATC_C Cuts at: 3852 4534 4901 3852 Size: 682 367 6155 Fragments arranged by size: 6155 682 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4525 5980 618 Size: 325 217 627 2738 1455 1842 Fragments arranged by size: 2738 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2259 3035 3937 4523 1391 Size: 868 776 902 586 4072 Fragments arranged by size: 4072 902 868 776 586 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2097 2313 3210 3249 3513 3744 3953 Size: 971 198 216 897 39 264 231 209 Cuts at: 3953 928 Size: 4179 Fragments arranged by size: 4179 971 897 264 231 216 209 198 39 BbvCI CC'TCA_GC Cuts at: 2583 3668 3843 2583 Size: 1085 175 5944 Fragments arranged by size: 5944 1085 175 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5395 5429 1903 Size: 34 50 34 3374 34 3678 Fragments arranged by size: 3678 3374 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5096 6306 5096 Size: 1210 5994 BclI T'GATC_A Cuts at: 1548 2497 3721 1548 Size: 949 1224 5031 Fragments arranged by size: 5031 1224 949 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 7204 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5815 136 Size: 107 122 71 631 605 4143 1525 Fragments arranged by size: 4143 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2041 2485 7199 2041 Size: 444 4714 2046 Fragments arranged by size: 4714 2046 444 BlpI GC'TnA_GC Cuts at: 4621 4621 Size: 7204 Bme1580I G_kGCm'C Cuts at: 1790 3035 5265 6421 1790 Size: 1245 2230 1156 2573 Fragments arranged by size: 2573 2230 1245 1156 BmgBI CAC'GTC Cuts at: 1318 4063 1318 Size: 2745 4459 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2369 4935 5893 Size: 416 53 83 399 24 952 2566 958 Cuts at: 5893 442 Size: 1753 Fragments arranged by size: 2566 1753 958 952 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7204 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3011 3588 4421 5864 1239 Size: 1772 577 833 1443 2579 Fragments arranged by size: 2579 1772 1443 833 577 Bpu10I CC'TnA_GC Cuts at: 2583 3668 3843 2583 Size: 1085 175 5944 Fragments arranged by size: 5944 1085 175 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5329 6194 6492 5329 Size: 865 298 6041 Fragments arranged by size: 6041 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2210 2281 5867 882 Size: 617 711 71 3586 2219 Fragments arranged by size: 3586 2219 711 617 71 BsaAI yAC'GTr Cuts at: 493 1204 4995 493 Size: 711 3791 2702 Fragments arranged by size: 3791 2702 711 BsaBI GATnn'nnATC Cuts at: 3857 7198 3857 Size: 3341 3863 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5011 5393 6028 275 Size: 53 83 186 936 3478 382 635 1451 Fragments arranged by size: 3478 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5637 6035 6309 6456 6867 5637 Size: 398 274 147 411 5974 Fragments arranged by size: 5974 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2051 2081 2954 2984 3002 Size: 30 165 30 648 30 873 30 18 Cuts at: 3002 3032 3983 4013 1178 Size: 30 951 30 4369 Fragments arranged by size: 4369 951 873 648 165 30 30 30 30 30 30 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2304 3010 1186 Size: 1118 706 5380 Fragments arranged by size: 5380 1118 706 BseYI C'CCAG_C Cuts at: 2054 3511 6407 6903 2054 Size: 1457 2896 496 2355 Fragments arranged by size: 2896 2355 1457 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2458 1163 Size: 573 722 5909 Fragments arranged by size: 5909 722 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4654 5415 6443 1540 Size: 275 186 2653 761 1028 2301 Fragments arranged by size: 2653 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3937 3967 5265 5350 6421 3937 Size: 30 1298 85 1071 4720 Fragments arranged by size: 4720 1298 1071 85 30 BsmI GAATG_Cn' Cuts at: 2965 4426 4715 4808 2965 Size: 1461 289 93 5361 Fragments arranged by size: 5361 1461 289 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2259 3035 3937 3967 4523 5265 5350 Size: 399 469 776 902 30 556 742 85 Cuts at: 5350 6421 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 902 776 742 556 469 399 85 30 BspEI T'CCGG_A Cuts at: 6867 6867 Size: 7204 BspHI T'CATG_A Cuts at: 2340 5093 6823 2340 Size: 2753 1730 2721 Fragments arranged by size: 2753 2721 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2569 3785 4559 844 Size: 1725 1216 774 3489 Fragments arranged by size: 3489 1725 1216 774 BsrBI CCG'CTC Cuts at: 1394 1823 4350 5091 1394 Size: 429 2527 741 3507 Fragments arranged by size: 3507 2527 741 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2397 3078 4103 5699 5873 65 Size: 1183 1149 681 1025 1596 174 1396 Fragments arranged by size: 1596 1396 1183 1149 1025 681 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5848 6035 1700 Size: 110 4038 187 2869 Fragments arranged by size: 4038 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7204 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7204 BssSI C'ACGA_G Cuts at: 2049 5264 6276 2049 Size: 3215 1012 2977 Fragments arranged by size: 3215 2977 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3962 794 Size: 3168 4036 BstBI TT'CG_AA Cuts at: 4368 6008 4368 Size: 1640 5564 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7204 BstXI CCAn_nnnn'nTGG Cuts at: 4501 4501 Size: 7204 BstYI r'GATC_y Cuts at: 1972 2041 2485 3852 4015 4534 4901 5286 5303 Size: 69 444 1367 163 519 367 385 17 Cuts at: 5303 6755 6841 6853 7199 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1367 519 444 385 367 346 163 86 69 17 12 BtgI C'CryG_G Cuts at: 513 1065 3690 3848 513 Size: 552 2625 158 3869 Fragments arranged by size: 3869 2625 552 158 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6465 Fragments arranged by size: 6465 683 56 BtsI GCAGTG_nn' Cuts at: 2409 4720 5514 5534 2409 Size: 2311 794 20 4079 Fragments arranged by size: 4079 2311 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7204 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3363 3398 529 Size: 35 2799 35 4335 Fragments arranged by size: 4335 2799 35 35 DraI TTT'AAA Cuts at: 4508 4508 Size: 7204 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6211 809 Size: 507 4895 1802 Fragments arranged by size: 4895 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2952 3460 4147 Size: 54 1425 50 275 18 1122 508 687 Cuts at: 4147 4329 4651 5540 7169 8 Size: 182 322 889 1629 43 Fragments arranged by size: 1629 1425 1122 889 687 508 322 275 182 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4651 1537 Size: 275 2839 4090 Fragments arranged by size: 4090 2839 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2069 2904 5134 1353 Size: 716 835 2230 3423 Fragments arranged by size: 3423 2230 835 716 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5791 6165 6311 387 Size: 1064 138 4202 374 146 1280 Fragments arranged by size: 4202 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3935 3935 Size: 7204 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 3011 3507 3588 3806 4057 4421 Size: 393 492 887 496 81 218 251 364 Cuts at: 4421 5267 5864 6651 6919 1239 Size: 846 597 787 268 1524 Fragments arranged by size: 1524 887 846 787 597 496 492 393 364 268 251 218 81 EcoO109I rG'GnC_Cy Cuts at: 2059 2245 3032 2059 Size: 186 787 6231 Fragments arranged by size: 6231 787 186 EcoRI G'AATT_C Cuts at: 3155 4513 4891 3155 Size: 1358 378 5468 Fragments arranged by size: 5468 1358 378 EcoRV GAT'ATC Cuts at: 2338 2338 Size: 7204 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2114 3372 3664 3787 4187 2114 Size: 1258 292 123 400 5131 Fragments arranged by size: 5131 1258 400 292 123 FspI TGC'GCA Cuts at: 5710 5710 Size: 7204 HaeII r_GCGC'y Cuts at: 1678 6351 1678 Size: 4673 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 2207 2239 3002 Size: 32 135 32 479 32 124 32 763 Cuts at: 3002 3034 3551 3583 5845 5877 5919 5951 1373 Size: 32 517 32 2262 32 42 32 2626 Fragments arranged by size: 2626 2262 763 517 479 135 124 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4548 4794 669 Size: 988 281 2610 246 3079 Fragments arranged by size: 3079 2610 988 281 246 HindIII A'AGCT_T Cuts at: 748 2106 748 Size: 1358 5846 HpaI GTT'AAC Cuts at: 4794 4794 Size: 7204 KpnI G_GTAC'C Cuts at: 4529 4529 Size: 7204 MfeI C'AATT_G Cuts at: 4803 4803 Size: 7204 MluI A'CGCG_T Cuts at: 7080 7080 Size: 7204 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6318 6502 1305 Size: 5013 184 2007 Fragments arranged by size: 5013 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2954 3462 4149 4331 10 Size: 54 1425 343 1122 508 687 182 2883 Fragments arranged by size: 2883 1425 1122 687 508 343 182 54 MslI CAynn'nnrTG Cuts at: 518 1166 2402 2904 4096 4448 5162 5521 5680 Size: 648 1236 502 1192 352 714 359 159 Cuts at: 5680 518 Size: 2042 Fragments arranged by size: 2042 1236 1192 714 648 502 359 352 159 MspA1I CmG'CkG Cuts at: 1606 2632 3511 4620 5297 6445 6690 1606 Size: 1026 879 1109 677 1148 245 2120 Fragments arranged by size: 2120 1148 1109 1026 879 677 245 NcoI C'CATG_G Cuts at: 513 1065 3690 3848 513 Size: 552 2625 158 3869 Fragments arranged by size: 3869 2625 552 158 NdeI CA'TA_TG Cuts at: 387 3685 387 Size: 3298 3906 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7204 NotI GC'GGCC_GC Cuts at: 4651 4651 Size: 7204 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 7204 NspI r_CATG'y Cuts at: 1132 1229 2240 2675 2903 4562 6107 1132 Size: 97 1011 435 228 1659 1545 2229 Fragments arranged by size: 2229 1659 1545 1011 435 228 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7204 PciI A'CATG_T Cuts at: 6103 6103 Size: 7204 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7204 PmeI GTTT'AAAC Cuts at: 4508 4508 Size: 7204 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5273 5305 6786 6818 5273 Size: 32 1481 32 5659 Fragments arranged by size: 5659 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 2059 Size: 7204 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7204 PsiI TTA'TAA Cuts at: 4774 4774 Size: 7204 PspOMI G'GGCC_C Cuts at: 3031 3031 Size: 7204 PspXI vC'TCGA_Gb Cuts at: 2696 2696 Size: 7204 PstI C_TGCA'G Cuts at: 830 4556 830 Size: 3726 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7204 PvuII CAG'CTG Cuts at: 2632 3511 2632 Size: 879 6325 SacI G_AGCT'C Cuts at: 3937 3937 Size: 7204 SalI G'TCGA_C Cuts at: 1936 4546 1936 Size: 2610 4594 SbfI CC_TGCA'GG Cuts at: 4556 4556 Size: 7204 ScaI AGT'ACT Cuts at: 5452 7164 5452 Size: 1712 5492 SexAI A'CCwGG_T Cuts at: 2937 4023 2937 Size: 1086 6118 SfcI C'TryA_G Cuts at: 826 1045 3624 4552 5687 6368 6559 826 Size: 219 2579 928 1135 681 191 1471 Fragments arranged by size: 2579 1471 1135 928 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7204 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7204 SmaI CCC'GGG Cuts at: 1872 4361 4531 1872 Size: 2489 170 4545 Fragments arranged by size: 4545 2489 170 SmlI C'TyrA_G Cuts at: 820 1017 1943 2696 5308 6209 6471 820 Size: 197 926 753 2612 901 262 1553 Fragments arranged by size: 2612 1553 926 901 753 262 197 SnaBI TAC'GTA Cuts at: 493 4995 493 Size: 4502 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7204 SphI G_CATG'C Cuts at: 1132 2240 4562 1132 Size: 1108 2322 3774 Fragments arranged by size: 3774 2322 1108 SspI AAT'ATT Cuts at: 5 52 3541 5128 5 Size: 47 3489 1587 2081 Fragments arranged by size: 3489 2081 1587 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7204 StyI C'CwwG_G Cuts at: 513 1065 2478 2530 3114 3463 3690 3848 4407 Size: 552 1413 52 584 349 227 158 559 Cuts at: 4407 4643 513 Size: 236 3074 Fragments arranged by size: 3074 1413 584 559 552 349 236 227 158 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2234 5231 5248 5401 5586 6031 325 Size: 1255 654 2997 17 153 185 445 1498 Fragments arranged by size: 2997 1498 1255 654 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4309 5450 7162 Size: 274 80 33 51 1443 2332 1141 1712 Cuts at: 7162 96 Size: 138 Fragments arranged by size: 2332 1712 1443 1141 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7164 7196 7164 Size: 32 7172 Tth111I GACn'n_nGTC Cuts at: 1316 2221 1316 Size: 905 6299 XbaI T'CTAG_A Cuts at: 4540 4540 Size: 7204 XhoI C'TCGA_G Cuts at: 1943 2696 1943 Size: 753 6451 XmaI C'CCGG_G Cuts at: 1870 4359 4529 1870 Size: 2489 170 4545 Fragments arranged by size: 4545 2489 170 XmnI GAAnn'nnTTC Cuts at: 5333 5333 Size: 7204 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5012 276 Size: 53 83 186 936 3478 2468 Fragments arranged by size: 3478 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI