Restriction map for
pFN21AA1625
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AviII AvrII
BanI BbeI BbvCI BcgI BfrI
BlnI BsaBI BsgI BsiWI BsmI
Bsp1407I BspDI BsrGI BssHII Bst1107I
BstAPI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PmeI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfoI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 1428 TCCGGA T^CCGGA
AcyI 666 GACGCC G[GA]^CG[CT]C
AflIII 1412 ACATGT A^C[GA][CT]GT
AgeI 556 ACCGGT A^CCGGT
ApaI 2107 GGGCCC GGGCC^C
AspHI 1837 GAGCTC G[AT]GC[AT]^C
AspI 2043 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 780 CACGTG CAC^GTG
BbsI 490 GAAGAC GAAGAC(2/6)
BclI 1889 TGATCA T^GATCA
BglI 501 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 513 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 513 GCTCAGC GC^T[ACGT]AGC
BpuAI 490 GAAGAC GAAGAC(2/6)
BsaAI 780 CACGTG [CT]AC^GT[GA]
BsaHI 666 GACGCC G[GA]^CG[CT]C
BseAI 1428 TCCGGA T^CCGGA
BsiEI 1258 CGACCG CG[GA][CT]^CG
BsiHKAI 1837 GAGCTC G[AT]GC[AT]^C
BsmBI 1999 GAGACG CGTCTC(1/5)
BspEI 1428 TCCGGA T^CCGGA
BspHI 1025 TCATGA T^CATGA
BspLU11I 1412 ACATGT A^CATGT
BsrDI 1192 CATTGC GCAATG(2/0)
BssSI 416 CACGAG CACGAG(-5/-1)
BstBI 635 TTCGAA TT^CGAA
CelII 513 GCTCAGC GC^T[ACGT]AGC
CfoI 2099 GCGC GCG^C
EarI 1384 CTCTTC CTCTTC(1/4)
EcoNI 2154 CCTTCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 850 GAATTC G^AATTC
FbaI 1889 TGATCA T^GATCA
HhaI 2099 GCGC GCG^C
Hin1I 666 GACGCC G[GA]^CG[CT]C
HinP1I 2099 GCGC G^CGC
Ksp632I 1384 CTCTTC CTCTTC(1/4)
MroI 1428 TCCGGA T^CCGGA
NcoI 1503 CCATGG C^CATGG
NdeI 2198 CATATG CA^TATG
NspI 1412 ACATGT [GA]CATG^[CT]
NspV 635 TTCGAA TT^CGAA
PflFI 2043 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 556 ACCGGT A^CCGGT
PmaCI 780 CACGTG CAC^GTG
PmlI 780 CACGTG CAC^GTG
RcaI 1025 TCATGA T^CATGA
SacI 1837 GAGCTC GAGCT^C
SapI 1383 GCTCTTC GCTCTTC(1/4)
SfiI 500 GGCCTGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 635 TTCGAA TT^CGAA
SgrAI 555 CACCGGTG C[GA]^CCGG[CT]G
SmaI 10 CCCGGG CCC^GGG
SmlI 1045 CTCAAG C^T[CT][GA]AG
SspI 1405 AATATT AAT^ATT
Tth111I 2043 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 10 CCCGGG C^CCGGG
XmaI 10 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 947 GTAC GT^AC
1648 GTAC
AhdI 1450 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1924 GACTGGAAGTC
AspEI 1450 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1924 GACTGGAAGTC
AvaI 10 CCCGGG C^[CT]CG[GA]G
810 CTCGGG
BalI 390 TGGCCA TGG^CCA
1328 TGGCCA
BamHI 1622 GGATCC G^GATCC
1691 GGATCC
BciVI 334 GGATAC GTATCC(6/5)
797 GGATAC
BfaI 1132 CTAG C^TAG
1627 CTAG
BglII 1128 AGATCT A^GATCT
1578 AGATCT
BsaI 884 GAGACC GGTCTC(1/5)
2248 GAGACC
BsoBI 10 CCCGGG C^[CT]CG[GA]G
810 CTCGGG
BsrBI 511 CCGCTC CCGCTC(-3/-3)
2062 CCGCTC
BsrFI 433 ACCGGC [GA]^CCGG[CT]
556 ACCGGT
Cfr10I 433 ACCGGC [GA]^CCGG[CT]
556 ACCGGT
DraII 2106 AGGGCCC [GA]G^G[ACGT]CC[CT]
2107 GGGCCCC
DraIII 1468 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1951 CACTGTGTG
Eam1105I 1450 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1924 GACTGGAAGTC
EcoO109I 2106 AGGGCCC [GA]G^G[ACGT]CC[CT]
2107 GGGCCCC
FauI 2061 CCCGC CCCGC(4/6)
2255 GCGGG
HgaI 666 GACGC GACGC(5/10)
2137 GACGC
MaeI 1132 CTAG C^TAG
1627 CTAG
MluNI 390 TGGCCA TGG^CCA
1328 TGGCCA
MscI 390 TGGCCA TGG^CCA
1328 TGGCCA
MslI 231 CATTAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1200 CATCGAAGTG
PvuII 516 CAGCTG CAG^CTG
1549 CAGCTG
RsaI 947 GTAC GT^AC
1648 GTAC
Tsp45I 1735 GTCAC ^GT[GC]AC
2041 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI ApoI Asp700I AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I Van91I XhoII XmnI