(Circular) (Six-base) MAPSORT of: pFN21AA1625.seq Check: 4964 from: 1 to: 6976 pFN21AA1625 6976 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4786 278 Size: 53 83 186 936 3250 2468 Fragments arranged by size: 3250 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4319 1937 Size: 2382 4594 Acc65I G'GTAC_C Cuts at: 4297 4297 Size: 6976 AclI AA'CG_TT Cuts at: 1563 5103 5476 1563 Size: 3540 373 3063 Fragments arranged by size: 3540 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2080 2609 3161 3884 3971 5039 6423 6691 Size: 448 529 552 723 87 1068 1384 268 Cuts at: 6691 1632 Size: 1917 Fragments arranged by size: 1917 1384 1068 723 552 529 448 268 87 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6779 AflIII A'CryG_T Cuts at: 1203 3417 5875 6852 1203 Size: 2214 2458 977 1327 Fragments arranged by size: 2458 2214 1327 977 AgeI A'CCGG_T Cuts at: 2561 5807 2561 Size: 3246 3730 AhdI GACnn_n'nnGTC Cuts at: 3460 3934 5705 3460 Size: 474 1771 4731 Fragments arranged by size: 4731 1771 474 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6976 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3749 3995 1184 Size: 2565 246 4165 Fragments arranged by size: 4165 2565 246 AlwNI CAG_nnn'CTG Cuts at: 2381 2960 3070 3148 3559 4719 6291 2381 Size: 579 110 78 411 1160 1572 3066 Fragments arranged by size: 3066 1572 1160 579 411 110 78 ApaI G_GGCC'C Cuts at: 4116 4116 Size: 6976 ApaLI G'TGCA_C Cuts at: 5033 6189 5033 Size: 1156 5820 ApoI r'AATT_y Cuts at: 1493 2259 2855 3296 4285 4511 4663 6772 1493 Size: 766 596 441 989 226 152 2109 1697 Fragments arranged by size: 2109 1697 989 766 596 441 226 152 AseI AT'TA_AT Cuts at: 160 1032 5530 160 Size: 872 4498 1606 Fragments arranged by size: 4498 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6976 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2815 4301 1870 Size: 73 72 800 1486 4545 Fragments arranged by size: 4545 1486 800 73 72 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2332 2365 2332 Size: 33 6943 BamHI G'GATC_C Cuts at: 3627 3696 4306 4673 3627 Size: 69 610 367 5930 Fragments arranged by size: 5930 610 367 69 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4297 5752 618 Size: 325 217 627 2510 1455 1842 Fragments arranged by size: 2510 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2886 3060 3092 3390 3846 4116 4295 1391 Size: 1495 174 32 298 456 270 179 4072 Fragments arranged by size: 4072 1495 456 298 270 179 174 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2502 928 Size: 971 603 5402 Fragments arranged by size: 5402 971 603 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5167 5201 1903 Size: 34 3230 34 3678 Fragments arranged by size: 3678 3230 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2333 2796 4868 6078 2333 Size: 463 2072 1210 3231 Fragments arranged by size: 3231 2072 1210 463 BclI T'GATC_A Cuts at: 1548 3894 1548 Size: 2346 4630 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2512 5587 136 Size: 107 122 71 631 605 840 3075 1525 Fragments arranged by size: 3075 1525 840 631 605 122 107 71 BglII A'GATC_T Cuts at: 3133 3583 6971 3133 Size: 450 3388 3138 Fragments arranged by size: 3388 3138 450 BlpI GC'TnA_GC Cuts at: 2519 4393 2519 Size: 1874 5102 Bme1580I G_kGCm'C Cuts at: 1790 3543 4116 5037 6193 1790 Size: 1753 573 921 1156 2573 Fragments arranged by size: 2573 1753 1156 921 573 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6976 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2995 3075 3805 Size: 416 53 83 399 24 1578 80 730 Cuts at: 3805 4059 4707 5665 442 Size: 254 648 958 1753 Fragments arranged by size: 1753 1578 958 730 648 416 399 254 83 80 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6976 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4085 4085 Size: 6976 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2020 2595 2796 3116 3805 3908 5636 1239 Size: 781 575 201 320 689 103 1728 2579 Fragments arranged by size: 2579 1728 781 689 575 320 201 103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3035 5101 5966 6264 3035 Size: 2066 865 298 3747 Fragments arranged by size: 3747 2066 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2883 4247 5639 882 Size: 617 1384 1364 1392 2219 Fragments arranged by size: 2219 1392 1384 1364 617 BsaAI yAC'GTr Cuts at: 493 1204 2787 4767 493 Size: 711 1583 1980 2702 Fragments arranged by size: 2702 1980 1583 711 BsaBI GATnn'nnATC Cuts at: 6970 6970 Size: 6976 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2672 4783 5165 5800 Size: 53 83 186 936 1139 2111 382 635 Cuts at: 5800 275 Size: 1451 Fragments arranged by size: 2111 1451 1139 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2336 2561 3433 3768 4185 5409 5807 6081 6228 Size: 225 872 335 417 1224 398 274 147 Cuts at: 6228 6639 2336 Size: 411 2673 Fragments arranged by size: 2673 1224 872 417 411 398 335 274 225 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6751 Fragments arranged by size: 6751 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2592 3119 3248 3761 4265 1186 Size: 1406 527 129 513 504 3897 Fragments arranged by size: 3897 1406 527 513 504 129 BseYI C'CCAG_C Cuts at: 2937 3059 3559 6179 6675 2937 Size: 122 500 2620 496 3238 Fragments arranged by size: 3238 2620 500 496 122 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6403 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3266 4426 5187 6215 1540 Size: 275 186 1265 1160 761 1028 2301 Fragments arranged by size: 2301 1265 1160 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3846 5037 5122 6193 3846 Size: 1191 85 1071 4629 Fragments arranged by size: 4629 1191 1071 85 BsmI GAATG_Cn' Cuts at: 4487 4580 4487 Size: 93 6883 BsmBI CGTCTCn'nnnn_ Cuts at: 3998 3998 Size: 6976 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2886 3060 3092 3390 3543 3846 4116 Size: 399 1096 174 32 298 153 303 270 Cuts at: 4116 4295 5037 5122 6193 1391 Size: 179 742 85 1071 2174 Fragments arranged by size: 2174 1096 1071 742 399 303 298 270 179 174 153 85 32 BspEI T'CCGG_A Cuts at: 3433 6639 3433 Size: 3206 3770 BspHI T'CATG_A Cuts at: 3030 4865 6595 3030 Size: 1835 1730 3411 Fragments arranged by size: 3411 1835 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2580 4184 4265 4331 844 Size: 1736 1604 81 66 3489 Fragments arranged by size: 3489 1736 1604 81 66 BsrBI CCG'CTC Cuts at: 1394 1823 2518 4069 4863 1394 Size: 429 695 1551 794 3507 Fragments arranged by size: 3507 1551 794 695 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3196 5471 5645 65 Size: 1183 1948 2275 174 1396 Fragments arranged by size: 2275 1948 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2438 2561 5620 5807 1700 Size: 110 628 123 3059 187 2869 Fragments arranged by size: 3059 2869 628 187 123 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6976 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6976 BssSI C'ACGA_G Cuts at: 2421 5036 6048 2421 Size: 2615 1012 3349 Fragments arranged by size: 3349 2615 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6976 BstBI TT'CG_AA Cuts at: 2641 5780 2641 Size: 3139 3837 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6976 BstYI r'GATC_y Cuts at: 1972 2046 2353 3133 3583 3627 3696 4306 4673 Size: 74 307 780 450 44 69 610 367 Cuts at: 4673 5058 5075 6527 6613 6625 6971 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 780 610 450 385 367 346 307 86 74 69 44 17 12 BtgI C'CryG_G Cuts at: 513 1065 3301 3508 4245 513 Size: 552 2236 207 737 3244 Fragments arranged by size: 3244 2236 737 552 207 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6237 Fragments arranged by size: 6237 683 56 BtsI GCAGTG_nn' Cuts at: 2150 2924 3074 4492 5286 5306 2150 Size: 774 150 1418 794 20 3820 Fragments arranged by size: 3820 1418 794 774 150 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6976 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6941 DraI TTT'AAA Cuts at: 4280 4280 Size: 6976 DraIII CAC_nnn'GTG Cuts at: 3478 3961 3478 Size: 483 6493 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5983 809 Size: 507 4667 1802 Fragments arranged by size: 4667 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2395 2692 3333 Size: 54 1425 50 275 18 565 297 641 Cuts at: 3333 3963 4423 5312 6941 8 Size: 630 460 889 1629 43 Fragments arranged by size: 1629 1425 889 641 630 565 460 297 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4423 1537 Size: 275 2611 4090 Fragments arranged by size: 4090 2611 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3395 4906 1353 Size: 2042 1511 3423 Fragments arranged by size: 3423 2042 1511 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3499 3709 5563 5937 6083 387 Size: 1064 138 1910 210 1854 374 146 1280 Fragments arranged by size: 1910 1854 1280 1064 374 210 146 138 EcoICRI GAG'CTC Cuts at: 3844 3844 Size: 6976 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2020 2080 2595 2609 2796 3116 3161 Size: 393 388 60 515 14 187 320 45 Cuts at: 3161 3805 3884 3908 3971 5039 5636 6423 6691 Size: 644 79 24 63 1068 597 787 268 Cuts at: 6691 1239 Size: 1524 Fragments arranged by size: 1524 1068 787 644 597 515 393 388 320 268 187 79 63 60 45 24 14 EcoNI CCTnn'n_nnAGG Cuts at: 4163 4163 Size: 6976 EcoO109I rG'GnC_Cy Cuts at: 4112 4113 4112 Size: 1 6975 EcoRI G'AATT_C Cuts at: 2855 4285 4663 2855 Size: 1430 378 5168 Fragments arranged by size: 5168 1430 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2107 2107 Size: 6976 FspI TGC'GCA Cuts at: 5482 5482 Size: 6976 HaeII r_GCGC'y Cuts at: 1678 6123 1678 Size: 4445 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2794 2826 3123 3155 3210 Size: 32 135 32 1222 32 297 32 55 Cuts at: 3210 3242 3446 3478 3504 3536 3575 3607 3920 Size: 32 204 32 26 32 39 32 313 Cuts at: 3920 3952 5617 5649 5691 5723 1373 Size: 32 1665 32 42 32 2626 Fragments arranged by size: 2626 1665 1222 313 297 204 135 55 42 39 32 32 32 32 32 32 32 32 32 32 32 26 HincII GTy'rAC Cuts at: 669 1657 1938 4320 4566 669 Size: 988 281 2382 246 3079 Fragments arranged by size: 3079 2382 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6976 HpaI GTT'AAC Cuts at: 4566 4566 Size: 6976 KpnI G_GTAC'C Cuts at: 4301 4301 Size: 6976 MfeI C'AATT_G Cuts at: 4575 4575 Size: 6976 MluI A'CGCG_T Cuts at: 6852 6852 Size: 6976 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6090 6274 1305 Size: 4785 184 2007 Fragments arranged by size: 4785 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2397 3335 10 Size: 54 1425 343 565 938 3651 Fragments arranged by size: 3651 1425 938 565 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2240 3209 4934 5293 5452 518 Size: 648 1074 969 1725 359 159 2042 Fragments arranged by size: 2042 1725 1074 969 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2523 2635 3070 3556 4392 5069 6217 6462 Size: 917 112 435 486 836 677 1148 245 Cuts at: 6462 1606 Size: 2120 Fragments arranged by size: 2120 1148 917 836 677 486 435 245 112 NcoI C'CATG_G Cuts at: 513 1065 3508 513 Size: 552 2443 3981 Fragments arranged by size: 3981 2443 552 NdeI CA'TA_TG Cuts at: 387 4204 387 Size: 3817 3159 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6976 NotI GC'GGCC_GC Cuts at: 4423 4423 Size: 6976 NspI r_CATG'y Cuts at: 1132 1229 3421 4334 5879 1132 Size: 97 2192 913 1545 2229 Fragments arranged by size: 2229 2192 1545 913 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6976 PciI A'CATG_T Cuts at: 3417 5875 3417 Size: 2458 4518 PflMI CCAn_nnn'nTGG Cuts at: 1108 2350 2404 3342 3478 1108 Size: 1242 54 938 136 4606 Fragments arranged by size: 4606 1242 938 136 54 PmeI GTTT'AAAC Cuts at: 4280 4280 Size: 6976 PmlI CAC'GTG Cuts at: 2787 2787 Size: 6976 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5045 5077 6558 6590 5045 Size: 32 1481 32 5431 Fragments arranged by size: 5431 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6976 PsiI TTA'TAA Cuts at: 4546 4546 Size: 6976 PspOMI G'GGCC_C Cuts at: 4112 4112 Size: 6976 PstI C_TGCA'G Cuts at: 830 2462 3705 3828 4041 4328 830 Size: 1632 1243 123 213 287 3478 Fragments arranged by size: 3478 1632 1243 287 213 123 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6976 PvuII CAG'CTG Cuts at: 2523 3556 2523 Size: 1033 5943 SacI G_AGCT'C Cuts at: 3846 3846 Size: 6976 SalI G'TCGA_C Cuts at: 1936 4318 1936 Size: 2382 4594 SapI GCTCTTCn'nnn_ Cuts at: 3395 3395 Size: 6976 SbfI CC_TGCA'GG Cuts at: 4328 4328 Size: 6976 ScaI AGT'ACT Cuts at: 5224 6936 5224 Size: 1712 5264 SfcI C'TryA_G Cuts at: 826 1045 2458 2666 3701 3824 4037 4098 4324 Size: 219 1413 208 1035 123 213 61 226 Cuts at: 4324 5459 6140 6331 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1413 1135 1035 681 226 219 213 208 191 123 61 SfiI GGCCn_nnn'nGGCC Cuts at: 2512 2512 Size: 6976 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6976 SgrAI Cr'CCGG_yG Cuts at: 1700 2561 1700 Size: 861 6115 SmaI CCC'GGG Cuts at: 1872 2017 4303 1872 Size: 145 2286 4545 Fragments arranged by size: 4545 2286 145 SmlI C'TyrA_G Cuts at: 820 1017 1943 3050 5080 5981 6243 820 Size: 197 926 1107 2030 901 262 1553 Fragments arranged by size: 2030 1553 1107 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4767 493 Size: 4274 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6976 SphI G_CATG'C Cuts at: 1132 4334 1132 Size: 3202 3774 SspI AAT'ATT Cuts at: 5 52 3412 4900 5 Size: 47 3360 1488 2081 Fragments arranged by size: 3360 2081 1488 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6976 StyI C'CwwG_G Cuts at: 513 1065 3014 3508 4072 4415 513 Size: 552 1949 494 564 343 3074 Fragments arranged by size: 3074 1949 564 552 494 343 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3280 5003 5020 5173 5358 5803 325 Size: 1255 1700 1723 17 153 185 445 1498 Fragments arranged by size: 1723 1700 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2951 3652 5222 Size: 274 80 33 51 1443 974 701 1570 Cuts at: 5222 6934 96 Size: 1712 138 Fragments arranged by size: 1712 1570 1443 974 701 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2171 2203 6936 6968 2171 Size: 32 4733 32 2179 Fragments arranged by size: 4733 2179 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4051 1316 Size: 2735 4241 XbaI T'CTAG_A Cuts at: 4312 4312 Size: 6976 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6976 XmaI C'CCGG_G Cuts at: 1870 2015 4301 1870 Size: 145 2286 4545 Fragments arranged by size: 4545 2286 145 XmnI GAAnn'nnTTC Cuts at: 2090 2859 3833 5105 2090 Size: 769 974 1272 3961 Fragments arranged by size: 3961 1272 974 769 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4784 276 Size: 53 83 186 936 3250 2468 Fragments arranged by size: 3250 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI XcmI