Restriction map for
pFN21AA1597
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AspEI
AspI AviII BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoT22I FbaI FseI
FspI HaeII HapII HgaI Hin1I
HpaI HpaII KasI KspI MaeII
MamI MluI MroI MspI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI TaqI Tth111I TthHB8I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 2138 GGTACC G^GTACC
Alw44I 2263 GTGCAC G^TGCAC
ApaLI 2263 GTGCAC G^TGCAC
AseI 1204 ATTAAT AT^TAAT
AsnI 1204 ATTAAT AT^TAAT
Asp718I 2138 GGTACC G^GTACC
AspHI 2263 GTGCAC G[AT]GC[AT]^C
AvaI 339 CCCGAG C^[CT]CG[GA]G
AvrII 2070 CCTAGG C^CTAGG
BalI 105 TGGCCA TGG^CCA
BamHI 767 GGATCC G^GATCC
BanII 755 GGGCTC G[GA]GC[CT]^C
BciVI 2046 GGATAC GTATCC(6/5)
BglII 2448 AGATCT A^GATCT
BlnI 2070 CCTAGG C^CTAGG
BlpI 208 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 208 GCTGAGC GC^T[ACGT]AGC
BsaI 1400 GAGACC GGTCTC(1/5)
BsiHKAI 2263 GTGCAC G[AT]GC[AT]^C
BsoBI 339 CCCGAG C^[CT]CG[GA]G
CelII 208 GCTGAGC GC^T[ACGT]AGC
DraI 693 TTTAAA TTT^AAA
EaeI 105 TGGCCA [CT]^GGCC[GA]
EcoNI 2070 CCTAGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1260 GAATTC G^AATTC
EcoRV 163 GATATC GAT^ATC
FauI 15 GCGGG CCCGC(4/6)
HindIII 2242 AAGCTT A^AGCTT
KpnI 2138 GGTACC GGTAC^C
MluNI 105 TGGCCA TGG^CCA
MscI 105 TGGCCA TGG^CCA
PpuMI 634 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1244 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1204 ATTAAT AT^TAAT
PstI 959 CTGCAG CTGCA^G
SapI 307 GAAGAGC GCTCTTC(1/4)
SspI 1774 AATATT AAT^ATT
XcmI 2501 CCAACCAATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 768 GATCC GGATC(4/5)
767 GGATC
AlwNI 85 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2228 CAGTCCCTG
AvaII 635 GGTCC G^G[AT]CC
2484 GGACC
BanI 1380 GGTGCC G^G[CT][GA]CC
2138 GGTACC
BmyI 755 GGGCTC G[AGT]GC[ACT]^C
2263 GTGCAC
Bsp1286I 755 GGGCTC G[AGT]GC[ACT]^C
2263 GTGCAC
BstAPI 609 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2161 GCACCAGTTGC
BstYI 767 GGATCC [GA]^GATC[CT]
2448 AGATCT
DraII 193 AGGCCCC [GA]G^G[ACGT]CC[CT]
634 GGGTCCC
DsaI 153 CCATGG C^C[GA][CT]GG
999 CCATGG
EcoO109I 193 AGGCCCC [GA]G^G[ACGT]CC[CT]
634 GGGTCCC
HincII 262 GTCAAC GT[CT]^[GA]AC
564 GTCAAC
HindII 262 GTCAAC GT[CT]^[GA]AC
564 GTCAAC
MfeI 1787 CAATTG C^AATTG
2128 CAATTG
MflI 767 GGATCC [GA]^GATC[CT]
2448 AGATCT
MslI 2268 CATCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2418 CACTATGGTG
MunI 1787 CAATTG C^AATTG
2128 CAATTG
NcoI 153 CCATGG C^CATGG
999 CCATGG
NdeI 1151 CATATG CA^TATG
1883 CATATG
NspI 945 GCATGT [GA]CATG^[CT]
1818 GCATGT
PvuII 88 CAGCTG CAG^CTG
206 CAGCTG
SfcI 959 CTGCAG C^T[GA][CT]AG
2384 CTGTAG
SmlI 772 CTCAAG C^T[CT][GA]AG
1143 CTCAAG
XbaI 1396 TCTAGA T^CTAGA
1488 TCTAGA
XhoII 767 GGATCC [GA]^GATC[CT]
2448 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I BbsI BbvI BfaI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI BsmI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XmnI