Restriction map for
pFN21AA1478
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AviII BalI
BamHI BanI BbeI BbrPI BcgI
BclI BfrI BglII BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 622 GTAGAC GT^[AC][GT]AC
AflIII 1659 ACATGT A^C[GA][CT]GT
AhdI 1057 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 682 GGGCCC GGGCC^C
AspEI 1057 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 197 GTGCTC G[AT]GC[AT]^C
AvrII 1854 CCTAGG C^CTAGG
BanII 682 GGGCCC G[GA]GC[CT]^C
BciVI 3 GGATAC GTATCC(6/5)
BcnI 51 CCGGG CC^[GC]GG
BglI 1480 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1854 CCTAGG C^CTAGG
BsaAI 224 CACGTA [CT]AC^GT[GA]
BsaI 963 GGTCTC GGTCTC(1/5)
BsiHKAI 197 GTGCTC G[AT]GC[AT]^C
BspLU11I 1659 ACATGT A^CATGT
BsrDI 407 GCAATG GCAATG(2/0)
BstAPI 39 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1277 TTCGAA TT^CGAA
BstXI 1377 CCAAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 1725 GGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1057 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1725 GGGACCT [GA]G^G[ACGT]CC[CT]
FauI 680 GCGGG CCCGC(4/6)
HapII 51 CCGG C^CGG
HgaI 1566 GCGTC GACGC(5/10)
HincII 1568 GTCAAC GT[CT]^[GA]AC
HindII 1568 GTCAAC GT[CT]^[GA]AC
HpaII 51 CCGG C^CGG
MaeII 225 ACGT A^CGT
MspI 51 CCGG C^CGG
NciI 51 CCGGG CC^[GC]GG
NspV 1277 TTCGAA TT^CGAA
PflMI 144 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1725 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 985 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 617 CTGCAG CTGCA^G
SfuI 1277 TTCGAA TT^CGAA
SphI 219 GCATGC GCATG^C
StuI 1143 AGGCCT AGG^CCT
Van91I 144 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 922 AAATTT [GA]^AATT[CT]
1195 AAATTC
AlwNI 182 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
659 CAGTGACTG
ApoI 922 AAATTT [GA]^AATT[CT]
1195 AAATTC
Asp700I 516 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1291 GAACCTCTTC
BbsI 516 GAAGAC GAAGAC(2/6)
1351 GAAGAC
BbvCI 1224 CCTCAGC CCTCAGC(-5/-2)
265 GCTGAGG
BpmI 796 CTGGAG CTGGAG(16/14)
1606 CTGGAG
BpuAI 516 GAAGAC GAAGAC(2/6)
1351 GAAGAC
BseRI 1222 CTCCTC GAGGAG(10/8)
355 GAGGAG
BspHI 1427 TCATGA T^CATGA
1625 TCATGA
BstYI 165 GGATCT [GA]^GATC[CT]
1154 GGATCT
EcoRV 463 GATATC GAT^ATC
1240 GATATC
HaeII 548 GGCGCT [GA]GCGC^[CT]
1586 AGCGCC
MflI 165 GGATCT [GA]^GATC[CT]
1154 GGATCT
MslI 1092 CATTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1626 CATGATGGTG
NspI 219 GCATGC [GA]CATG^[CT]
1659 ACATGT
PvuII 301 CAGCTG CAG^CTG
793 CAGCTG
RcaI 1427 TCATGA T^CATGA
1625 TCATGA
SmlI 1756 CTCAAG C^T[CT][GA]AG
1891 CTCAAG
XhoII 165 GGATCT [GA]^GATC[CT]
1154 GGATCT
XmnI 516 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1291 GAACCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BfaI BmyI BsaJI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I