(Circular) (Six-base) MAPSORT of: pFN21AA1478.seq Check: 5373 from: 1 to: 6601 pFN21AA1478 6601 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4411 278 Size: 53 83 186 936 2875 2468 Fragments arranged by size: 2875 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2628 3944 1937 Size: 691 1316 4594 Fragments arranged by size: 4594 1316 691 Acc65I G'GTAC_C Cuts at: 3922 3922 Size: 6601 AclI AA'CG_TT Cuts at: 1563 4728 5101 1563 Size: 3165 373 3063 Fragments arranged by size: 3165 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2240 2555 2966 4664 6048 6316 1632 Size: 608 315 411 1698 1384 268 1917 Fragments arranged by size: 1917 1698 1384 608 411 315 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6404 AflIII A'CryG_T Cuts at: 1203 3664 5500 6477 1203 Size: 2461 1836 977 1327 Fragments arranged by size: 2461 1836 1327 977 AgeI A'CCGG_T Cuts at: 5432 5432 Size: 6601 AhdI GACnn_n'nnGTC Cuts at: 3067 5330 3067 Size: 2263 4338 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6601 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6601 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2762 2794 2762 Size: 32 6569 AlwNI CAG_nnn'CTG Cuts at: 2192 2669 4344 5916 2192 Size: 477 1675 1572 2877 Fragments arranged by size: 2877 1675 1572 477 ApaI G_GGCC'C Cuts at: 2691 2691 Size: 6601 ApaLI G'TGCA_C Cuts at: 4658 5814 4658 Size: 1156 5445 ApoI r'AATT_y Cuts at: 1493 2927 3200 3910 4136 4288 6397 1493 Size: 1434 273 710 226 152 2109 1697 Fragments arranged by size: 2109 1697 1434 710 273 226 152 AseI AT'TA_AT Cuts at: 160 1032 5155 160 Size: 872 4123 1606 Fragments arranged by size: 4123 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6601 AvaI C'yCGr_G Cuts at: 1870 1943 3926 1870 Size: 73 1983 4545 Fragments arranged by size: 4545 1983 73 AvrII C'CTAG_G Cuts at: 3859 3859 Size: 6601 BamHI G'GATC_C Cuts at: 3931 4298 3931 Size: 367 6234 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3922 5377 618 Size: 325 217 627 2135 1455 1842 Fragments arranged by size: 2135 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2691 3920 1391 Size: 1300 1229 4072 Fragments arranged by size: 4072 1300 1229 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2528 3363 928 Size: 971 629 835 4166 Fragments arranged by size: 4166 971 835 629 BbvCI CC'TCA_GC Cuts at: 2271 3230 2271 Size: 959 5642 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4792 4826 1903 Size: 34 2855 34 3678 Fragments arranged by size: 3678 2855 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2002 4493 5703 2002 Size: 2491 1210 2900 Fragments arranged by size: 2900 2491 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6601 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3491 5212 136 Size: 107 122 71 631 605 1819 1721 1525 Fragments arranged by size: 1819 1721 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6596 6596 Size: 6601 BlpI GC'TnA_GC Cuts at: 4018 4018 Size: 6601 Bme1580I G_kGCm'C Cuts at: 1790 2691 2834 3534 4662 5818 1790 Size: 901 143 700 1128 1156 2573 Fragments arranged by size: 2573 1156 1128 901 700 143 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6601 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3539 3737 4332 Size: 416 53 83 399 24 2122 198 595 Cuts at: 4332 5290 442 Size: 958 1753 Fragments arranged by size: 2122 1753 958 595 416 399 198 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6601 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2339 3471 2339 Size: 1132 5469 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2822 3632 5261 1239 Size: 1583 810 1629 2579 Fragments arranged by size: 2579 1629 1583 810 Bpu10I CC'TnA_GC Cuts at: 2271 3230 2271 Size: 959 5642 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3746 3881 4726 5591 5889 3746 Size: 135 845 865 298 4458 Fragments arranged by size: 4458 865 845 298 135 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2974 5264 882 Size: 617 1475 2290 2219 Fragments arranged by size: 2290 2219 1475 617 BsaAI yAC'GTr Cuts at: 493 1204 2231 4392 493 Size: 711 1027 2161 2702 Fragments arranged by size: 2702 2161 1027 711 BsaBI GATnn'nnATC Cuts at: 6595 6595 Size: 6601 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4408 4790 5425 275 Size: 53 83 186 936 2875 382 635 1451 Fragments arranged by size: 2875 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5034 5432 5706 5853 6264 5034 Size: 398 274 147 411 5371 Fragments arranged by size: 5371 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2762 2792 1178 Size: 30 165 30 1359 30 4987 Fragments arranged by size: 4987 1359 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2375 3218 1186 Size: 1189 843 4569 Fragments arranged by size: 4569 1189 843 BseYI C'CCAG_C Cuts at: 2155 2417 5804 6300 2155 Size: 262 3387 496 2456 Fragments arranged by size: 3387 2456 496 262 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6028 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4051 4812 5840 1540 Size: 275 186 2050 761 1028 2301 Fragments arranged by size: 2301 2050 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2206 4662 4747 5818 2206 Size: 2456 85 1071 2989 Fragments arranged by size: 2989 2456 1071 85 BsmI GAATG_Cn' Cuts at: 2346 3009 3057 4112 4205 2346 Size: 663 48 1055 93 4742 Fragments arranged by size: 4742 1055 663 93 48 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2206 2691 2834 3534 3920 4662 4747 Size: 399 416 485 143 700 386 742 85 Cuts at: 4747 5818 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 700 485 416 399 386 143 85 BspEI T'CCGG_A Cuts at: 6264 6264 Size: 6601 BspHI T'CATG_A Cuts at: 3432 3630 4490 6220 3432 Size: 198 860 1730 3813 Fragments arranged by size: 3813 1730 860 198 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3956 844 Size: 3112 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4488 1394 Size: 429 2665 3507 Fragments arranged by size: 3507 2665 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2419 5096 5270 65 Size: 1183 1171 2677 174 1396 Fragments arranged by size: 2677 1396 1183 1171 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5245 5432 1700 Size: 110 3435 187 2869 Fragments arranged by size: 3435 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6601 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6601 BssSI C'ACGA_G Cuts at: 4661 5673 4661 Size: 1012 5589 BstAPI GCAn_nnn'nTGC Cuts at: 794 2050 794 Size: 1256 5345 BstBI TT'CG_AA Cuts at: 3283 5405 3283 Size: 2122 4479 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6601 BstXI CCAn_nnnn'nTGG Cuts at: 3389 3389 Size: 6601 BstYI r'GATC_y Cuts at: 1972 2170 3159 3931 4298 4683 4700 6152 6238 Size: 198 989 772 367 385 17 1452 86 Cuts at: 6238 6250 6596 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 989 772 385 367 346 198 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5110 Fragments arranged by size: 5110 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5862 Fragments arranged by size: 5862 683 56 BtsI GCAGTG_nn' Cuts at: 4117 4911 4931 4117 Size: 794 20 5787 Fragments arranged by size: 5787 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6601 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6566 DraI TTT'AAA Cuts at: 3905 3905 Size: 6601 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5608 809 Size: 507 4292 1802 Fragments arranged by size: 4292 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4048 4937 6566 Size: 54 1425 50 275 18 2218 889 1629 Cuts at: 6566 8 Size: 43 Fragments arranged by size: 2218 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4048 1537 Size: 275 2236 4090 Fragments arranged by size: 4090 2236 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2515 2534 3306 4531 1353 Size: 1162 19 772 1225 3423 Fragments arranged by size: 3423 1225 1162 772 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5188 5562 5708 387 Size: 1064 138 3599 374 146 1280 Fragments arranged by size: 3599 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2555 2822 2966 3632 4664 5261 Size: 393 608 315 267 144 666 1032 597 Cuts at: 5261 6048 6316 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1032 787 666 608 597 393 315 268 267 144 EcoO109I rG'GnC_Cy Cuts at: 3731 3731 Size: 6601 EcoRI G'AATT_C Cuts at: 3910 4288 3910 Size: 378 6223 EcoRV GAT'ATC Cuts at: 2470 3247 2470 Size: 777 5824 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3296 3317 3296 Size: 21 6580 FspI TGC'GCA Cuts at: 5107 5107 Size: 6601 HaeII r_GCGC'y Cuts at: 1678 2557 3595 5748 1678 Size: 879 1038 2153 2531 Fragments arranged by size: 2531 2153 1038 879 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2354 2386 2492 2524 3053 Size: 32 135 32 782 32 106 32 529 Cuts at: 3053 3085 5242 5274 5316 5348 1373 Size: 32 2157 32 42 32 2626 Fragments arranged by size: 2626 2157 782 529 135 106 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3575 3945 4191 669 Size: 988 281 1637 370 246 3079 Fragments arranged by size: 3079 1637 988 370 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6601 HpaI GTT'AAC Cuts at: 4191 4191 Size: 6601 KpnI G_GTAC'C Cuts at: 3926 3926 Size: 6601 MfeI C'AATT_G Cuts at: 4200 4200 Size: 6601 MluI A'CGCG_T Cuts at: 6477 6477 Size: 6601 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5715 5899 1305 Size: 4410 184 2007 Fragments arranged by size: 4410 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4779 Fragments arranged by size: 4779 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3101 3635 4559 4918 5077 518 Size: 648 1935 534 924 359 159 2042 Fragments arranged by size: 2042 1935 924 648 534 359 159 MspA1I CmG'CkG Cuts at: 1606 2308 2800 2994 4017 4694 5842 6087 1606 Size: 702 492 194 1023 677 1148 245 2120 Fragments arranged by size: 2120 1148 1023 702 677 492 245 194 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5110 Fragments arranged by size: 5110 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6601 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6601 NotI GC'GGCC_GC Cuts at: 4048 4048 Size: 6601 NspI r_CATG'y Cuts at: 1132 1229 2228 3668 3959 5504 1132 Size: 97 999 1440 291 1545 2229 Fragments arranged by size: 2229 1545 1440 999 291 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6601 PciI A'CATG_T Cuts at: 3664 5500 3664 Size: 1836 4765 PflMI CCAn_nnn'nTGG Cuts at: 1108 2155 1108 Size: 1047 5554 PmeI GTTT'AAAC Cuts at: 3905 3905 Size: 6601 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2762 2794 3784 3816 4670 4702 6183 6215 2762 Size: 32 990 32 854 32 1481 32 3148 Fragments arranged by size: 3148 1481 990 854 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3731 3731 Size: 6601 PshAI GACnn'nnGTC Cuts at: 1613 2994 1613 Size: 1381 5220 PsiI TTA'TAA Cuts at: 4171 4171 Size: 6601 PspOMI G'GGCC_C Cuts at: 2687 2687 Size: 6601 PstI C_TGCA'G Cuts at: 830 2626 3953 830 Size: 1796 1327 3478 Fragments arranged by size: 3478 1796 1327 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6601 PvuII CAG'CTG Cuts at: 2308 2800 2308 Size: 492 6109 SalI G'TCGA_C Cuts at: 1936 3943 1936 Size: 2007 4594 SbfI CC_TGCA'GG Cuts at: 3953 3953 Size: 6601 ScaI AGT'ACT Cuts at: 4849 6561 4849 Size: 1712 4889 SfcI C'TryA_G Cuts at: 826 1045 2622 2661 3259 3949 5084 5765 5956 Size: 219 1577 39 598 690 1135 681 191 Cuts at: 5956 826 Size: 1471 Fragments arranged by size: 1577 1471 1135 690 681 598 219 191 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6601 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6601 SmaI CCC'GGG Cuts at: 1872 3928 1872 Size: 2056 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3761 3896 4705 5606 5868 820 Size: 197 926 1818 135 809 901 262 1553 Fragments arranged by size: 1818 1553 926 901 809 262 197 135 SnaBI TAC'GTA Cuts at: 493 4392 493 Size: 3899 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6601 SphI G_CATG'C Cuts at: 1132 2228 3959 1132 Size: 1096 1731 3774 Fragments arranged by size: 3774 1731 1096 SspI AAT'ATT Cuts at: 5 52 4525 5 Size: 47 4473 2081 Fragments arranged by size: 4473 2081 47 StuI AGG'CCT Cuts at: 1517 3150 1517 Size: 1633 4968 StyI C'CwwG_G Cuts at: 513 1065 2004 2765 3579 3859 4040 513 Size: 552 939 761 814 280 181 3074 Fragments arranged by size: 3074 939 814 761 552 280 181 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4628 4645 4798 4983 5428 325 Size: 1255 3048 17 153 185 445 1498 Fragments arranged by size: 3048 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4847 6559 96 Size: 274 80 33 51 1443 2870 1712 138 Fragments arranged by size: 2870 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3780 3812 6561 6593 3780 Size: 32 2749 32 3788 Fragments arranged by size: 3788 2749 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6601 XbaI T'CTAG_A Cuts at: 3937 3937 Size: 6601 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6601 XmaI C'CCGG_G Cuts at: 1870 3926 1870 Size: 2056 4545 XmnI GAAnn'nnTTC Cuts at: 2525 3300 4730 2525 Size: 775 1430 4396 Fragments arranged by size: 4396 1430 775 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4409 276 Size: 53 83 186 936 2875 2468 Fragments arranged by size: 2875 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI