Restriction map for
pFN21AA1464
(Linear) (Six-base) MAPSORT of: KIAA1464.seq Check: 5864 from: 1 to: 1860
KIAA1464.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 544 1860
Size: 544 1316
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1611 1860
Size: 1611 249
AfeI AGC'GCT
Cuts at: 0 946 1860
Size: 946 914
AlwNI CAG_nnn'CTG
Cuts at: 0 102 123 900 1743 1860
Size: 102 21 777 843 117
Fragments arranged by size:
843 777 117 102 21
ApoI r'AATT_y
Cuts at: 0 1593 1860
Size: 1593 267
AvaI C'yCGr_G
Cuts at: 0 21 456 1808 1860
Size: 21 435 1352 52
Fragments arranged by size:
1352 435 52 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1314 1347 1860
Size: 1314 33 513
Fragments arranged by size:
1314 513 33
BanI G'GyrC_C
Cuts at: 0 532 1429 1860
Size: 532 897 431
Fragments arranged by size:
897 532 431
BanII G_rGCy'C
Cuts at: 0 175 1057 1860
Size: 175 882 803
Fragments arranged by size:
882 803 175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1158 1192 1860
Size: 1158 34 668
Fragments arranged by size:
1158 668 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 137 1860
Size: 137 1723
BglI GCCn_nnn'nGGC
Cuts at: 0 920 1667 1860
Size: 920 747 193
Fragments arranged by size:
920 747 193
BlpI GC'TnA_GC
Cuts at: 0 103 1860
Size: 103 1757
Bme1580I G_kGCm'C
Cuts at: 0 718 744 1860
Size: 718 26 1116
Fragments arranged by size:
1116 718 26
BmgBI CAC'GTC
Cuts at: 0 501 1860
Size: 501 1359
BmrI ACTGGGnnnn_n'
Cuts at: 0 1115 1657 1860
Size: 1115 542 203
Fragments arranged by size:
1115 542 203
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1311 1860
Size: 1311 549
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 928 985 1674 1860
Size: 189 739 57 689 186
Fragments arranged by size:
739 689 189 186 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 979 1860
Size: 356 623 881
Fragments arranged by size:
881 623 356
BsaI GGTCTCn'nnnn_
Cuts at: 0 205 928 1339 1860
Size: 205 723 411 521
Fragments arranged by size:
723 521 411 205
BsaHI Gr'CG_yC
Cuts at: 0 377 641 868 1175 1860
Size: 377 264 227 307 685
Fragments arranged by size:
685 377 307 264 227
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1075 1105 1860
Size: 1075 30 755
Fragments arranged by size:
1075 755 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 42 444 973 1218 1233 1236 1239 1242
Size: 42 402 529 245 15 3 3 3
Cuts at: 1242 1860
Size: 618
Fragments arranged by size:
618 529 402 245 42 15 3 3
3
BseYI C'CCAG_C
Cuts at: 0 28 67 941 1096 1159 1222 1316 1860
Size: 28 39 874 155 63 63 94 544
Fragments arranged by size:
874 544 155 94 63 63 39 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1202 1860
Size: 1202 658
BsiHKAI G_wGCw'C
Cuts at: 0 971 1057 1382 1860
Size: 971 86 325 478
Fragments arranged by size:
971 478 325 86
BsmI GAATG_Cn'
Cuts at: 0 441 1860
Size: 441 1419
BsmBI CGTCTCn'nnnn_
Cuts at: 0 491 1860
Size: 491 1369
Bsp1286I G_dGCh'C
Cuts at: 0 175 718 744 971 1057 1382 1860
Size: 175 543 26 227 86 325 478
Fragments arranged by size:
543 478 325 227 175 86 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 297 544 1457 1751 1860
Size: 297 247 913 294 109
Fragments arranged by size:
913 297 294 247 109
BsrDI GCAATG_nn'
Cuts at: 0 1176 1860
Size: 1176 684
BsrFI r'CCGG_y
Cuts at: 0 107 1001 1860
Size: 107 894 859
Fragments arranged by size:
894 859 107
BssSI C'ACGA_G
Cuts at: 0 793 1860
Size: 793 1067
BstAPI GCAn_nnn'nTGC
Cuts at: 0 765 1860
Size: 765 1095
BstEII G'GTnAC_C
Cuts at: 0 421 1860
Size: 421 1439
BstXI CCAn_nnnn'nTGG
Cuts at: 0 475 565 1860
Size: 475 90 1295
Fragments arranged by size:
1295 475 90
BstYI r'GATC_y
Cuts at: 0 890 1860
Size: 890 970
BtgI C'CryG_G
Cuts at: 0 131 426 821 1860
Size: 131 295 395 1039
Fragments arranged by size:
1039 395 295 131
BtsI GCAGTG_nn'
Cuts at: 0 723 770 1860
Size: 723 47 1090
Fragments arranged by size:
1090 723 47
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 810 845 1401 1436 1860
Size: 810 35 556 35 424
Fragments arranged by size:
810 556 424 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1039 1461 1860
Size: 1039 422 399
Fragments arranged by size:
1039 422 399
EaeI y'GGCC_r
Cuts at: 0 465 555 590 1542 1668 1860
Size: 465 90 35 952 126 192
Fragments arranged by size:
952 465 192 126 90 35
EarI CTCTTCn'nnn_
Cuts at: 0 333 991 1266 1860
Size: 333 658 275 594
Fragments arranged by size:
658 594 333 275
EcoICRI GAG'CTC
Cuts at: 0 1055 1860
Size: 1055 805
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 928 985 1611 1674 1860
Size: 189 739 57 626 63 186
Fragments arranged by size:
739 626 189 186 63 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1777 1860
Size: 1777 83
EcoO109I rG'GnC_Cy
Cuts at: 0 1446 1860
Size: 1446 414
HaeII r_GCGC'y
Cuts at: 0 68 114 123 948 1724 1860
Size: 68 46 9 825 776 136
Fragments arranged by size:
825 776 136 68 46 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 247 279 488 520 875 907 1860
Size: 247 32 209 32 355 32 953
Fragments arranged by size:
953 355 247 209 32 32 32
HincII GTy'rAC
Cuts at: 0 138 381 519 1860
Size: 138 243 138 1341
Fragments arranged by size:
1341 243 138 138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1316 1860
Size: 1316 544
MscI TGG'CCA
Cuts at: 0 467 557 1670 1860
Size: 467 90 1113 190
Fragments arranged by size:
1113 467 190 90
MslI CAynn'nnrTG
Cuts at: 0 254 416 431 1609 1788 1860
Size: 254 162 15 1178 179 72
Fragments arranged by size:
1178 254 179 162 72 15
MspA1I CmG'CkG
Cuts at: 0 133 156 1163 1860
Size: 133 23 1007 697
Fragments arranged by size:
1007 697 133 23
NaeI GCC'GGC
Cuts at: 0 109 1003 1860
Size: 109 894 857
Fragments arranged by size:
894 857 109
NcoI C'CATG_G
Cuts at: 0 426 1860
Size: 426 1434
NgoMIV G'CCGG_C
Cuts at: 0 107 1001 1860
Size: 107 894 859
Fragments arranged by size:
894 859 107
NspI r_CATG'y
Cuts at: 0 690 1137 1410 1860
Size: 690 447 273 450
Fragments arranged by size:
690 450 447 273
PasI CC'CwG_GG
Cuts at: 0 88 207 955 1773 1860
Size: 88 119 748 818 87
Fragments arranged by size:
818 748 119 88 87
PflMI CCAn_nnn'nTGG
Cuts at: 0 900 1860
Size: 900 960
PfoI T'CCnGG_A
Cuts at: 0 166 1860
Size: 166 1694
PpuMI rG'GwC_Cy
Cuts at: 0 1446 1860
Size: 1446 414
PstI C_TGCA'G
Cuts at: 0 620 773 1454 1619 1860
Size: 620 153 681 165 241
Fragments arranged by size:
681 620 241 165 153
PvuII CAG'CTG
Cuts at: 0 1163 1860
Size: 1163 697
SacI G_AGCT'C
Cuts at: 0 1057 1860
Size: 1057 803
SacII CC_GC'GG
Cuts at: 0 134 1860
Size: 134 1726
SfcI C'TryA_G
Cuts at: 0 616 769 1164 1450 1615 1860
Size: 616 153 395 286 165 245
Fragments arranged by size:
616 395 286 245 165 153
SmaI CCC'GGG
Cuts at: 0 23 1860
Size: 23 1837
SmlI C'TyrA_G
Cuts at: 0 371 994 1860
Size: 371 623 866
Fragments arranged by size:
866 623 371
StuI AGG'CCT
Cuts at: 0 614 1817 1860
Size: 614 1203 43
Fragments arranged by size:
1203 614 43
StyI C'CwwG_G
Cuts at: 0 176 426 564 704 1860
Size: 176 250 138 140 1156
Fragments arranged by size:
1156 250 176 140 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 292 955 1236 1860
Size: 292 663 281 624
Fragments arranged by size:
663 624 292 281
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1020 1052 1687 1719 1860
Size: 1020 32 635 32 141
Fragments arranged by size:
1020 635 141 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 433 1860
Size: 433 1427
XmaI C'CCGG_G
Cuts at: 0 21 1860
Size: 21 1839
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApoI AvaI BaeI BanI
BanII BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI BtgI BtsI CspCI
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PasI PflMI PfoI PpuMI PstI PvuII
SacI SacII SfcI SmaI SmlI StuI StyI TaqII
TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BamHI BbeI BbsI BbvCI BclI BfrBI BglII
BmtI Bpu10I BsaAI BsaBI BsaWI BsiEI BsiWI BspEI
BspHI BsrBI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI
ClaI DraI DraIII EagI EciI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI
ZraI