(Circular) (Six-base) MAPSORT of: pFN21AA1342.seq Check: 9957 from: 1 to: 5980 pFN21AA1342 5980 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3790 278 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3323 1937 Size: 1386 4594 Acc65I G'GTAC_C Cuts at: 3301 3301 Size: 5980 AclI AA'CG_TT Cuts at: 1563 4107 4480 1563 Size: 2544 373 3063 Fragments arranged by size: 3063 2544 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2050 2427 2826 2844 4043 5427 5695 1632 Size: 418 377 399 18 1199 1384 268 1917 Fragments arranged by size: 1917 1384 1199 418 399 377 268 18 AflII C'TTAA_G Cuts at: 820 1017 2174 820 Size: 197 1157 4626 Fragments arranged by size: 4626 1157 197 AflIII A'CryG_T Cuts at: 1203 4879 5856 1203 Size: 3676 977 1327 Fragments arranged by size: 3676 1327 977 AgeI A'CCGG_T Cuts at: 4811 4811 Size: 5980 AhdI GACnn_n'nnGTC Cuts at: 4709 4709 Size: 5980 AleI CACnn'nnGTG Cuts at: 1166 2054 1166 Size: 888 5092 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2128 1184 Size: 944 5036 AlwNI CAG_nnn'CTG Cuts at: 3723 5295 3723 Size: 1572 4408 ApaLI G'TGCA_C Cuts at: 4037 5193 4037 Size: 1156 4824 ApoI r'AATT_y Cuts at: 1493 2033 2272 3122 3289 3515 3667 5776 1493 Size: 540 239 850 167 226 152 2109 1697 Fragments arranged by size: 2109 1697 850 540 239 226 167 152 AseI AT'TA_AT Cuts at: 160 1032 4534 160 Size: 872 3502 1606 Fragments arranged by size: 3502 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 AvaI C'yCGr_G Cuts at: 1870 1943 2788 3305 1870 Size: 73 845 517 4545 Fragments arranged by size: 4545 845 517 73 BamHI G'GATC_C Cuts at: 2650 3310 3677 2650 Size: 660 367 4953 Fragments arranged by size: 4953 660 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3301 4756 618 Size: 325 217 627 1514 1455 1842 Fragments arranged by size: 1842 1514 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3299 1391 Size: 1908 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2077 2850 3035 928 Size: 971 178 773 185 3873 Fragments arranged by size: 3873 971 773 185 178 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4171 4205 1903 Size: 34 2234 34 3678 Fragments arranged by size: 3678 2234 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2679 3872 5082 2679 Size: 1193 1210 3577 Fragments arranged by size: 3577 1210 1193 BclI T'GATC_A Cuts at: 1548 1548 Size: 5980 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4591 136 Size: 107 122 71 631 605 2919 1525 Fragments arranged by size: 2919 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2302 3225 5975 2302 Size: 923 2750 2307 Fragments arranged by size: 2750 2307 923 BlpI GC'TnA_GC Cuts at: 3397 3397 Size: 5980 Bme1580I G_kGCm'C Cuts at: 1790 4041 5197 1790 Size: 2251 1156 2573 Fragments arranged by size: 2573 2251 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5980 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3711 4669 442 Size: 416 53 83 399 24 2294 958 1753 Fragments arranged by size: 2294 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5980 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2378 4640 1239 Size: 1139 2262 2579 Fragments arranged by size: 2579 2262 1139 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4105 4970 5268 4105 Size: 865 298 4817 Fragments arranged by size: 4817 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2660 4643 882 Size: 617 1161 1983 2219 Fragments arranged by size: 2219 1983 1161 617 BsaAI yAC'GTr Cuts at: 493 1204 3265 3771 493 Size: 711 2061 506 2702 Fragments arranged by size: 2702 2061 711 506 BsaBI GATnn'nnATC Cuts at: 5974 5974 Size: 5980 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3787 4169 4804 275 Size: 53 83 186 936 2254 382 635 1451 Fragments arranged by size: 2254 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2963 4413 4811 5085 5232 5643 2963 Size: 1450 398 274 147 411 3300 Fragments arranged by size: 3300 1450 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5755 Fragments arranged by size: 5755 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2139 1186 Size: 953 5027 BseYI C'CCAG_C Cuts at: 2464 5183 5679 2464 Size: 2719 496 2765 Fragments arranged by size: 2765 2719 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3207 1163 Size: 573 1471 3936 Fragments arranged by size: 3936 1471 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3430 4191 5219 1540 Size: 275 186 1429 761 1028 2301 Fragments arranged by size: 2301 1429 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3216 3270 4041 4126 5197 3216 Size: 54 771 85 1071 3999 Fragments arranged by size: 3999 1071 771 85 54 BsmI GAATG_Cn' Cuts at: 2368 3491 3584 2368 Size: 1123 93 4764 Fragments arranged by size: 4764 1123 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3216 3270 3299 4041 4126 5197 1391 Size: 399 1426 54 29 742 85 1071 2174 Fragments arranged by size: 2174 1426 1071 742 399 85 54 29 BspEI T'CCGG_A Cuts at: 2963 5643 2963 Size: 2680 3300 BspHI T'CATG_A Cuts at: 3869 5599 3869 Size: 1730 4250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3335 844 Size: 2491 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3867 1394 Size: 429 2044 3507 Fragments arranged by size: 3507 2044 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2276 4475 4649 65 Size: 1183 1028 2199 174 1396 Fragments arranged by size: 2199 1396 1183 1028 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4624 4811 1700 Size: 110 2814 187 2869 Fragments arranged by size: 2869 2814 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5980 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5980 BssSI C'ACGA_G Cuts at: 4040 5052 4040 Size: 1012 4968 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5980 BstBI TT'CG_AA Cuts at: 4784 4784 Size: 5980 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5980 BstXI CCAn_nnnn'nTGG Cuts at: 2056 2056 Size: 5980 BstYI r'GATC_y Cuts at: 1972 2061 2109 2287 2302 2650 2974 3225 3310 Size: 89 48 178 15 348 324 251 85 Cuts at: 3310 3677 4062 4079 5531 5617 5629 5975 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 348 346 324 251 178 89 86 85 48 17 15 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2536 2550 513 Size: 552 939 532 14 3943 Fragments arranged by size: 3943 939 552 532 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5241 Fragments arranged by size: 5241 683 56 BtsI GCAGTG_nn' Cuts at: 3066 3496 4290 4310 3066 Size: 430 794 20 4736 Fragments arranged by size: 4736 794 430 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5945 DraI TTT'AAA Cuts at: 2421 3284 2421 Size: 863 5117 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4987 809 Size: 507 3671 1802 Fragments arranged by size: 3671 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3427 4316 5945 Size: 54 1425 50 275 18 1597 889 1629 Cuts at: 5945 8 Size: 43 Fragments arranged by size: 1629 1597 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3427 1537 Size: 275 1615 4090 Fragments arranged by size: 4090 1615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2442 2480 3910 1353 Size: 1089 38 1430 3423 Fragments arranged by size: 3423 1430 1089 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4567 4941 5087 387 Size: 1064 138 2978 374 146 1280 Fragments arranged by size: 2978 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2050 2378 2427 2826 2844 4043 4640 Size: 393 418 328 49 399 18 1199 597 Cuts at: 4640 5427 5695 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1199 787 597 418 399 393 328 268 49 18 EcoO109I rG'GnC_Cy Cuts at: 3100 3150 3100 Size: 50 5930 EcoRI G'AATT_C Cuts at: 2272 3289 3667 2272 Size: 1017 378 4585 Fragments arranged by size: 4585 1017 378 EcoRV GAT'ATC Cuts at: 2761 2761 Size: 5980 FspI TGC'GCA Cuts at: 4486 4486 Size: 5980 HaeII r_GCGC'y Cuts at: 1678 5127 1678 Size: 3449 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2101 2133 2546 2578 2745 Size: 32 135 32 529 32 413 32 167 Cuts at: 2745 2777 2820 2852 4621 4653 4695 4727 1373 Size: 32 43 32 1769 32 42 32 2626 Fragments arranged by size: 2626 1769 529 413 167 135 43 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3324 3570 669 Size: 988 281 1386 246 3079 Fragments arranged by size: 3079 1386 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5980 HpaI GTT'AAC Cuts at: 3570 3570 Size: 5980 KpnI G_GTAC'C Cuts at: 3305 3305 Size: 5980 MfeI C'AATT_G Cuts at: 3579 3579 Size: 5980 MluI A'CGCG_T Cuts at: 5856 5856 Size: 5980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5094 5278 1305 Size: 3789 184 2007 Fragments arranged by size: 3789 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4158 Fragments arranged by size: 4158 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2054 3938 4297 4456 518 Size: 648 888 1884 359 159 2042 Fragments arranged by size: 2042 1884 888 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2261 3396 4073 5221 5466 1606 Size: 655 1135 677 1148 245 2120 Fragments arranged by size: 2120 1148 1135 677 655 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2550 513 Size: 552 939 546 3943 Fragments arranged by size: 3943 939 552 546 NdeI CA'TA_TG Cuts at: 387 387 Size: 5980 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5980 NotI GC'GGCC_GC Cuts at: 3427 3427 Size: 5980 NspI r_CATG'y Cuts at: 1132 1229 2171 3338 4883 1132 Size: 97 942 1167 1545 2229 Fragments arranged by size: 2229 1545 1167 942 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5980 PciI A'CATG_T Cuts at: 4879 4879 Size: 5980 PflMI CCAn_nnn'nTGG Cuts at: 1108 2710 1108 Size: 1602 4378 PmeI GTTT'AAAC Cuts at: 3284 3284 Size: 5980 PmlI CAC'GTG Cuts at: 3265 3265 Size: 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 4081 5562 5594 4049 Size: 32 1481 32 4435 Fragments arranged by size: 4435 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3150 3150 Size: 5980 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5980 PsiI TTA'TAA Cuts at: 3550 3550 Size: 5980 PstI C_TGCA'G Cuts at: 830 3332 830 Size: 2502 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5980 PvuII CAG'CTG Cuts at: 2261 2261 Size: 5980 SalI G'TCGA_C Cuts at: 1936 3322 1936 Size: 1386 4594 SanDI GG'GwC_CC Cuts at: 3150 3150 Size: 5980 SbfI CC_TGCA'GG Cuts at: 3332 3332 Size: 5980 ScaI AGT'ACT Cuts at: 4228 5940 4228 Size: 1712 4268 SfcI C'TryA_G Cuts at: 826 1045 3328 4463 5144 5335 826 Size: 219 2283 1135 681 191 1471 Fragments arranged by size: 2283 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5980 SmaI CCC'GGG Cuts at: 1872 3307 1872 Size: 1435 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2174 4084 4985 5247 820 Size: 197 926 231 1910 901 262 1553 Fragments arranged by size: 1910 1553 926 901 262 231 197 SnaBI TAC'GTA Cuts at: 493 3771 493 Size: 3278 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5980 SphI G_CATG'C Cuts at: 1132 3338 1132 Size: 2206 3774 SspI AAT'ATT Cuts at: 5 52 3904 5 Size: 47 3852 2081 Fragments arranged by size: 3852 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5980 StyI C'CwwG_G Cuts at: 513 1065 2004 2550 2646 3419 513 Size: 552 939 546 96 773 3074 Fragments arranged by size: 3074 939 773 552 546 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2711 3173 4007 4024 4177 4362 4807 Size: 1255 1131 462 834 17 153 185 445 Cuts at: 4807 325 Size: 1498 Fragments arranged by size: 1498 1255 1131 834 462 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4226 5938 96 Size: 274 80 33 51 1443 2249 1712 138 Fragments arranged by size: 2249 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2053 2085 5940 5972 2053 Size: 32 3855 32 2061 Fragments arranged by size: 3855 2061 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5980 XbaI T'CTAG_A Cuts at: 3316 3316 Size: 5980 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5980 XmaI C'CCGG_G Cuts at: 1870 3305 1870 Size: 1435 4545 XmnI GAAnn'nnTTC Cuts at: 4109 4109 Size: 5980 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3788 276 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI