(Circular) (Six-base) MAPSORT of: pFN21AA1331.seq Check: 5853 from: 1 to: 6472 pFN21AA1331 6472 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4282 278 Size: 53 83 186 936 2746 2468 Fragments arranged by size: 2746 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3815 1937 Size: 1878 4594 Acc65I G'GTAC_C Cuts at: 3793 3793 Size: 6472 AclI AA'CG_TT Cuts at: 1563 4599 4972 1563 Size: 3036 373 3063 Fragments arranged by size: 3063 3036 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2105 2271 2595 3515 4535 5919 6187 1632 Size: 473 166 324 920 1020 1384 268 1917 Fragments arranged by size: 1917 1384 1020 920 473 324 268 166 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6275 AflIII A'CryG_T Cuts at: 1203 5371 6348 1203 Size: 4168 977 1327 Fragments arranged by size: 4168 1327 977 AgeI A'CCGG_T Cuts at: 2055 5303 2055 Size: 3248 3224 AhdI GACnn_n'nnGTC Cuts at: 5201 5201 Size: 6472 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6472 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2456 1184 Size: 1272 5200 AlwNI CAG_nnn'CTG Cuts at: 2333 3516 3769 4215 5787 2333 Size: 1183 253 446 1572 3018 Fragments arranged by size: 3018 1572 1183 446 253 ApaI G_GGCC'C Cuts at: 2080 2080 Size: 6472 ApaLI G'TGCA_C Cuts at: 4529 5685 4529 Size: 1156 5316 ApoI r'AATT_y Cuts at: 1493 3781 4007 4159 6268 1493 Size: 2288 226 152 2109 1697 Fragments arranged by size: 2288 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3252 5026 160 Size: 872 2220 1774 1606 Fragments arranged by size: 2220 1774 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2418 3198 3797 1870 Size: 73 130 345 780 599 4545 Fragments arranged by size: 4545 780 599 345 130 73 AvrII C'CTAG_G Cuts at: 3142 3142 Size: 6472 BamHI G'GATC_C Cuts at: 2336 3802 4169 2336 Size: 1466 367 4639 Fragments arranged by size: 4639 1466 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 2062 3415 3793 5248 Size: 325 217 627 246 29 1353 378 1455 Cuts at: 5248 618 Size: 1842 Fragments arranged by size: 1842 1455 1353 627 378 325 246 217 29 BanII G_rGCy'C Cuts at: 1391 2080 3791 1391 Size: 689 1711 4072 Fragments arranged by size: 4072 1711 689 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2491 928 Size: 971 592 4909 Fragments arranged by size: 4909 971 592 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4663 4697 1903 Size: 34 2726 34 3678 Fragments arranged by size: 3678 2726 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2939 4364 5574 2939 Size: 1425 1210 3837 Fragments arranged by size: 3837 1425 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6472 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5083 136 Size: 107 122 71 631 605 3411 1525 Fragments arranged by size: 3411 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6467 6467 Size: 6472 BlpI GC'TnA_GC Cuts at: 3889 3889 Size: 6472 Bme1580I G_kGCm'C Cuts at: 1790 2067 2080 4533 5689 1790 Size: 277 13 2453 1156 2573 Fragments arranged by size: 2573 2453 1156 277 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6472 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2369 4203 5161 Size: 416 53 83 399 24 952 1834 958 Cuts at: 5161 442 Size: 1753 Fragments arranged by size: 1834 1753 958 952 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6472 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2975 5132 1239 Size: 1736 2157 2579 Fragments arranged by size: 2579 2157 1736 Bpu10I CC'TnA_GC Cuts at: 3064 3064 Size: 6472 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4597 5462 5760 4597 Size: 865 298 5309 Fragments arranged by size: 5309 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5135 882 Size: 617 3636 2219 Fragments arranged by size: 3636 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4263 493 Size: 711 3059 2702 Fragments arranged by size: 3059 2702 711 BsaBI GATnn'nnATC Cuts at: 3576 6466 3576 Size: 2890 3582 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2599 4279 4661 5296 Size: 53 83 186 936 1066 1680 382 635 Cuts at: 5296 275 Size: 1451 Fragments arranged by size: 1680 1451 1066 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2055 3729 4905 5303 5577 5724 6135 2055 Size: 1674 1176 398 274 147 411 2392 Fragments arranged by size: 2392 1674 1176 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3631 3661 1178 Size: 30 165 30 2228 30 3989 Fragments arranged by size: 3989 2228 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2633 3216 1186 Size: 1447 583 4442 Fragments arranged by size: 4442 1447 583 BseYI C'CCAG_C Cuts at: 2766 5675 6171 2766 Size: 2909 496 3067 Fragments arranged by size: 3067 2909 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5899 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2056 2060 3922 4683 5711 1540 Size: 275 186 55 4 1862 761 1028 2301 Fragments arranged by size: 2301 1862 1028 761 275 186 55 4 BsiHKAI G_wGCw'C Cuts at: 4533 4618 5689 4533 Size: 85 1071 5316 Fragments arranged by size: 5316 1071 85 BsmI GAATG_Cn' Cuts at: 3983 4076 3983 Size: 93 6379 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2080 3791 4533 4618 5689 1391 Size: 399 277 13 1711 742 85 1071 2174 Fragments arranged by size: 2174 1711 1071 742 399 277 85 13 BspEI T'CCGG_A Cuts at: 3729 6135 3729 Size: 2406 4066 BspHI T'CATG_A Cuts at: 4361 6091 4361 Size: 1730 4742 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3827 844 Size: 2983 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4359 1394 Size: 429 2536 3507 Fragments arranged by size: 3507 2536 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3016 4967 5141 65 Size: 1183 1768 1951 174 1396 Fragments arranged by size: 1951 1768 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2055 5116 5303 1700 Size: 110 245 3061 187 2869 Fragments arranged by size: 3061 2869 245 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 96 Size: 1911 4561 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6472 BssSI C'ACGA_G Cuts at: 3614 4532 5544 3614 Size: 918 1012 4542 Fragments arranged by size: 4542 1012 918 BstAPI GCAn_nnn'nTGC Cuts at: 794 3718 794 Size: 2924 3548 BstBI TT'CG_AA Cuts at: 2303 5276 2303 Size: 2973 3499 BstEII G'GTnAC_C Cuts at: 1184 3353 1184 Size: 2169 4303 BstYI r'GATC_y Cuts at: 1972 2336 3513 3802 4169 4554 4571 6023 6109 Size: 364 1177 289 367 385 17 1452 86 Cuts at: 6109 6121 6467 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1177 385 367 364 346 289 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2544 2544 Size: 6472 BtgI C'CryG_G Cuts at: 513 1065 2244 3148 513 Size: 552 1179 904 3837 Fragments arranged by size: 3837 1179 904 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5733 Fragments arranged by size: 5733 683 56 BtsI GCAGTG_nn' Cuts at: 3007 3369 3988 4782 4802 3007 Size: 362 619 794 20 4677 Fragments arranged by size: 4677 794 619 362 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6472 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6437 DraI TTT'AAA Cuts at: 3776 3776 Size: 6472 DraIII CAC_nnn'GTG Cuts at: 2179 2179 Size: 6472 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5479 809 Size: 507 4163 1802 Fragments arranged by size: 4163 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2494 3919 4808 Size: 54 1425 50 275 18 664 1425 889 Cuts at: 4808 6437 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1425 889 664 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3919 1537 Size: 275 2107 4090 Fragments arranged by size: 4090 2107 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2081 2314 2571 2769 3731 4402 1353 Size: 728 233 257 198 962 671 3423 Fragments arranged by size: 3423 962 728 671 257 233 198 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2981 5059 5433 5579 387 Size: 1064 138 1392 2078 374 146 1280 Fragments arranged by size: 2078 1392 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2271 2595 2975 3515 4535 5132 Size: 393 473 166 324 380 540 1020 597 Cuts at: 5132 5919 6187 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1020 787 597 540 473 393 380 324 268 166 EcoO109I rG'GnC_Cy Cuts at: 2077 2843 2077 Size: 766 5706 EcoRI G'AATT_C Cuts at: 3781 4159 3781 Size: 378 6094 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3195 3195 Size: 6472 FspI TGC'GCA Cuts at: 4978 4978 Size: 6472 HaeII r_GCGC'y Cuts at: 1678 2098 5619 1678 Size: 420 3521 2531 Fragments arranged by size: 3521 2531 420 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5113 5145 5187 5219 1373 Size: 32 135 32 3541 32 42 32 2626 Fragments arranged by size: 3541 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3816 4062 669 Size: 988 281 1878 246 3079 Fragments arranged by size: 3079 1878 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6472 HpaI GTT'AAC Cuts at: 4062 4062 Size: 6472 KpnI G_GTAC'C Cuts at: 3797 3797 Size: 6472 MfeI C'AATT_G Cuts at: 4071 4071 Size: 6472 MluI A'CGCG_T Cuts at: 6348 6348 Size: 6472 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2893 5586 5770 1305 Size: 1588 2693 184 2007 Fragments arranged by size: 2693 2007 1588 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4650 Fragments arranged by size: 4650 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4430 4789 4948 518 Size: 648 3264 359 159 2042 Fragments arranged by size: 3264 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2230 3631 3888 4565 5713 5958 1606 Size: 624 1401 257 677 1148 245 2120 Fragments arranged by size: 2120 1401 1148 677 624 257 245 NcoI C'CATG_G Cuts at: 513 1065 2244 3148 513 Size: 552 1179 904 3837 Fragments arranged by size: 3837 1179 904 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6472 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6472 NotI GC'GGCC_GC Cuts at: 3919 3919 Size: 6472 NspI r_CATG'y Cuts at: 1132 1229 2353 3830 5375 1132 Size: 97 1124 1477 1545 2229 Fragments arranged by size: 2229 1545 1477 1124 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6472 PciI A'CATG_T Cuts at: 5371 5371 Size: 6472 PflMI CCAn_nnn'nTGG Cuts at: 1108 2766 1108 Size: 1658 4814 PfoI T'CCnGG_A Cuts at: 2339 2339 Size: 6472 PmeI GTTT'AAAC Cuts at: 3776 3776 Size: 6472 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4541 4573 6054 6086 4541 Size: 32 1481 32 4927 Fragments arranged by size: 4927 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2843 2843 Size: 6472 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6472 PsiI TTA'TAA Cuts at: 4042 4042 Size: 6472 PspOMI G'GGCC_C Cuts at: 2076 2076 Size: 6472 PspXI vC'TCGA_Gb Cuts at: 3198 3198 Size: 6472 PstI C_TGCA'G Cuts at: 830 3824 830 Size: 2994 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6472 PvuII CAG'CTG Cuts at: 2230 3631 2230 Size: 1401 5071 SalI G'TCGA_C Cuts at: 1936 3814 1936 Size: 1878 4594 SapI GCTCTTCn'nnn_ Cuts at: 2769 2769 Size: 6472 SbfI CC_TGCA'GG Cuts at: 3824 3824 Size: 6472 ScaI AGT'ACT Cuts at: 3322 4720 6432 3322 Size: 1398 1712 3362 Fragments arranged by size: 3362 1712 1398 SexAI A'CCwGG_T Cuts at: 2488 2488 Size: 6472 SfcI C'TryA_G Cuts at: 826 1045 2118 3820 4955 5636 5827 826 Size: 219 1073 1702 1135 681 191 1471 Fragments arranged by size: 1702 1471 1135 1073 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6472 SmaI CCC'GGG Cuts at: 1872 2075 3799 1872 Size: 203 1724 4545 Fragments arranged by size: 4545 1724 203 SmlI C'TyrA_G Cuts at: 820 1017 1943 3198 4576 5477 5739 820 Size: 197 926 1255 1378 901 262 1553 Fragments arranged by size: 1553 1378 1255 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4263 493 Size: 3770 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6472 SphI G_CATG'C Cuts at: 1132 3830 1132 Size: 2698 3774 SrfI GCCC'GGGC Cuts at: 2075 2075 Size: 6472 SspI AAT'ATT Cuts at: 5 52 4396 5 Size: 47 4344 2081 Fragments arranged by size: 4344 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6472 StyI C'CwwG_G Cuts at: 513 1065 2244 3142 3148 3911 513 Size: 552 1179 898 6 763 3074 Fragments arranged by size: 3074 1179 898 763 552 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4499 4516 4669 4854 5299 325 Size: 1255 2919 17 153 185 445 1498 Fragments arranged by size: 2919 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 2609 3320 Size: 274 80 33 51 1443 30 602 711 Cuts at: 3320 4718 6430 96 Size: 1398 1712 138 Fragments arranged by size: 1712 1443 1398 711 602 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2421 2453 3631 3663 6432 6464 2421 Size: 32 1178 32 2769 32 2429 Fragments arranged by size: 2769 2429 1178 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6472 XbaI T'CTAG_A Cuts at: 3808 3808 Size: 6472 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2242 2242 Size: 6472 XhoI C'TCGA_G Cuts at: 1943 3198 1943 Size: 1255 5217 XmaI C'CCGG_G Cuts at: 1870 2073 3797 1870 Size: 203 1724 4545 Fragments arranged by size: 4545 1724 203 XmnI GAAnn'nnTTC Cuts at: 3408 3753 4601 3408 Size: 345 848 5279 Fragments arranged by size: 5279 848 345 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4280 276 Size: 53 83 186 936 2746 2468 Fragments arranged by size: 2746 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SacII SanDI SfiI SfoI SwaI