Restriction map for
pFN21AA1265
(Circular) (Six-base) MAPSORT of: pFN21AA1265.seq Check: 517 from: 1 to: 7198
pFN21AA1265 7198 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5008 278
Size: 53 83 186 936 3472 2468
Fragments arranged by size:
3472 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4203 4541 1937
Size: 2266 338 4594
Fragments arranged by size:
4594 2266 338
Acc65I G'GTAC_C
Cuts at: 4519 4519
Size: 7198
AclI AA'CG_TT
Cuts at: 1563 5325 5698 1563
Size: 3762 373 3063
Fragments arranged by size:
3762 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 3213 3744 4033 4397 5261 6645 6913
Size: 492 1089 531 289 364 864 1384 268
Cuts at: 6913 1632
Size: 1917
Fragments arranged by size:
1917 1384 1089 864 531 492 364 289
268
AfeI AGC'GCT
Cuts at: 2109 2109
Size: 7198
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7001
AflIII A'CryG_T
Cuts at: 1203 2947 6097 7074 1203
Size: 1744 3150 977 1327
Fragments arranged by size:
3150 1744 1327 977
AgeI A'CCGG_T
Cuts at: 6029 6029
Size: 7198
AhdI GACnn_n'nnGTC
Cuts at: 5927 5927
Size: 7198
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3539 1184
Size: 2355 4843
AlwNI CAG_nnn'CTG
Cuts at: 3671 4304 4941 6513 3671
Size: 633 637 1572 4356
Fragments arranged by size:
4356 1572 637 633
ApaLI G'TGCA_C
Cuts at: 5255 6411 5255
Size: 1156 6042
ApoI r'AATT_y
Cuts at: 1493 2027 2153 2410 2731 3314 4417 4507 4733
Size: 534 126 257 321 583 1103 90 226
Cuts at: 4733 4885 6994 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1103 583 534 321 257 226
152 126 90
AseI AT'TA_AT
Cuts at: 160 1032 2301 5752 160
Size: 872 1269 3451 1606
Fragments arranged by size:
3451 1606 1269 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7198
AvaI C'yCGr_G
Cuts at: 1870 1943 4523 1870
Size: 73 2580 4545
Fragments arranged by size:
4545 2580 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2421 2454 2421
Size: 33 7165
BamHI G'GATC_C
Cuts at: 3977 4528 4895 3977
Size: 551 367 6280
Fragments arranged by size:
6280 551 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3053 3933 4519 5974 618
Size: 325 217 627 1266 880 586 1455 1842
Fragments arranged by size:
1842 1455 1266 880 627 586 325 217
BanII G_rGCy'C
Cuts at: 1391 4062 4517 1391
Size: 2671 455 4072
Fragments arranged by size:
4072 2671 455
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4114 928
Size: 971 2215 4012
Fragments arranged by size:
4012 2215 971
BbvCI CC'TCA_GC
Cuts at: 3911 3911
Size: 7198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5389 5423 1903
Size: 34 3452 34 3678
Fragments arranged by size:
3678 3452 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2449 5090 6300 2449
Size: 2641 1210 3347
Fragments arranged by size:
3347 2641 1210
BclI T'GATC_A
Cuts at: 1548 2569 2770 3406 4023 1548
Size: 1021 201 636 617 4723
Fragments arranged by size:
4723 1021 636 617 201
BfrBI ATG'CAT
Cuts at: 2518 2518
Size: 7198
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4226 5809 136
Size: 107 122 71 631 605 2554 1583 1525
Fragments arranged by size:
2554 1583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2890 2983 7193 2890
Size: 93 4210 2895
Fragments arranged by size:
4210 2895 93
BlpI GC'TnA_GC
Cuts at: 4615 4615
Size: 7198
Bme1580I G_kGCm'C
Cuts at: 1790 5259 6415 1790
Size: 3469 1156 2573
Fragments arranged by size:
3469 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7198
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2607 4929 5887
Size: 416 53 83 399 24 1190 2322 958
Cuts at: 5887 442
Size: 1753
Fragments arranged by size:
2322 1753 1190 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5858 1239
Size: 4619 2579
Bpu10I CC'TnA_GC
Cuts at: 3911 3911
Size: 7198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3671 5323 6188 6486 3671
Size: 1652 865 298 4383
Fragments arranged by size:
4383 1652 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2645 5861 882
Size: 617 1146 3216 2219
Fragments arranged by size:
3216 2219 1146 617
BsaAI yAC'GTr
Cuts at: 493 1204 4989 493
Size: 711 3785 2702
Fragments arranged by size:
3785 2702 711
BsaBI GATnn'nnATC
Cuts at: 2895 3000 4022 7192 2895
Size: 105 1022 3170 2901
Fragments arranged by size:
3170 2901 1022 105
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3366 5005 5387 6022
Size: 53 83 186 936 1833 1639 382 635
Cuts at: 6022 275
Size: 1451
Fragments arranged by size:
1833 1639 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5631 6029 6303 6450 6861 5631
Size: 398 274 147 411 5968
Fragments arranged by size:
5968 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2707 2737 1178
Size: 30 165 30 1304 30 5639
Fragments arranged by size:
5639 1304 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3821 4202 1186
Size: 2635 381 4182
Fragments arranged by size:
4182 2635 381
BseYI C'CCAG_C
Cuts at: 6401 6897 6401
Size: 496 6702
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3861 4092 1163
Size: 573 2125 231 4269
Fragments arranged by size:
4269 2125 573 231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4648 5409 6437 1540
Size: 275 186 2647 761 1028 2301
Fragments arranged by size:
2647 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5259 5344 6415 5259
Size: 85 1071 6042
Fragments arranged by size:
6042 1071 85
BsmI GAATG_Cn'
Cuts at: 2392 3943 4709 4802 2392
Size: 1551 766 93 4788
Fragments arranged by size:
4788 1551 766 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3357 3357
Size: 7198
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4062 4517 5259 5344 6415 1391
Size: 399 2272 455 742 85 1071 2174
Fragments arranged by size:
2272 2174 1071 742 455 399 85
BspEI T'CCGG_A
Cuts at: 6861 6861
Size: 7198
BspHI T'CATG_A
Cuts at: 2305 2866 3007 3853 4129 5087 6817 2305
Size: 561 141 846 276 958 1730 2686
Fragments arranged by size:
2686 1730 958 846 561 276 141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4553 844
Size: 3709 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5085 1394
Size: 429 3262 3507
Fragments arranged by size:
3507 3262 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2065 2074 2641 5693 5867 65
Size: 1183 817 9 567 3052 174 1396
Fragments arranged by size:
3052 1396 1183 817 567 174 9
BsrFI r'CCGG_y
Cuts at: 1700 1810 5842 6029 1700
Size: 110 4032 187 2869
Fragments arranged by size:
4032 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2818 2842 96
Size: 2722 24 4452
Fragments arranged by size:
4452 2722 24
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7198
BssSI C'ACGA_G
Cuts at: 5258 6270 5258
Size: 1012 6186
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2076 4070 4304 794
Size: 1282 1994 234 3688
Fragments arranged by size:
3688 1994 1282 234
BstBI TT'CG_AA
Cuts at: 6002 6002
Size: 7198
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7198
BstXI CCAn_nnnn'nTGG
Cuts at: 4450 4450
Size: 7198
BstYI r'GATC_y
Cuts at: 1972 2311 2890 2983 3977 4290 4528 4895 5280
Size: 339 579 93 994 313 238 367 385
Cuts at: 5280 5297 6749 6835 6847 7193 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 994 579 385 367 346 339
313 238 93 86 17 12
BstZ17I GTA'TAC
Cuts at: 4204 4204
Size: 7198
BtgI C'CryG_G
Cuts at: 513 1065 2095 3889 3958 513
Size: 552 1030 1794 69 3753
Fragments arranged by size:
3753 1794 1030 552 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6459
Fragments arranged by size:
6459 683 56
BtsI GCAGTG_nn'
Cuts at: 3541 4302 4714 5508 5528 3541
Size: 761 412 794 20 5211
Fragments arranged by size:
5211 794 761 412 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7198
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7163
DraI TTT'AAA
Cuts at: 2409 4502 2409
Size: 2093 5105
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6205 809
Size: 507 4889 1802
Fragments arranged by size:
4889 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2317 4645 5534
Size: 54 1425 50 275 18 487 2328 889
Cuts at: 5534 7163 8
Size: 1629 43
Fragments arranged by size:
2328 1629 1425 889 487 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4645 1537
Size: 275 2833 4090
Fragments arranged by size:
4090 2833 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3798 5128 1353
Size: 2445 1330 3423
Fragments arranged by size:
3423 2445 1330
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5785 6159 6305 387
Size: 1064 138 4196 374 146 1280
Fragments arranged by size:
4196 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 3213 3744 4033 4397 5261 5858
Size: 393 492 1089 531 289 364 864 597
Cuts at: 5858 6645 6913 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1089 864 787 597 531 492 393
364 289 268
EcoRI G'AATT_C
Cuts at: 4507 4885 4507
Size: 378 6820
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2933 3749 2933
Size: 816 6382
FspI TGC'GCA
Cuts at: 5704 5704
Size: 7198
HaeII r_GCGC'y
Cuts at: 1678 2111 6345 1678
Size: 433 4234 2531
Fragments arranged by size:
4234 2531 433
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2968 3000 5839 5871 5913
Size: 32 135 32 1396 32 2839 32 42
Cuts at: 5913 5945 1373
Size: 32 2626
Fragments arranged by size:
2839 2626 1396 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4542 4788 669
Size: 988 281 2604 246 3079
Fragments arranged by size:
3079 2604 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3635 748
Size: 2887 4311
HpaI GTT'AAC
Cuts at: 4788 4788
Size: 7198
KpnI G_GTAC'C
Cuts at: 4523 4523
Size: 7198
MfeI C'AATT_G
Cuts at: 4065 4197 4797 4065
Size: 132 600 6466
Fragments arranged by size:
6466 600 132
MluI A'CGCG_T
Cuts at: 7074 7074
Size: 7198
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6312 6496 1305
Size: 5007 184 2007
Fragments arranged by size:
5007 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2319 10
Size: 54 1425 343 487 4889
Fragments arranged by size:
4889 1425 487 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2079 2094 2325 2460 2517 2838 3006
Size: 648 913 15 231 135 57 321 168
Cuts at: 3006 3420 3426 3432 3444 3450 3551 3834 3858
Size: 414 6 6 12 6 101 283 24
Cuts at: 3858 3957 4238 4362 5156 5515 5674 518
Size: 99 281 124 794 359 159 2042
Fragments arranged by size:
2042 913 794 648 414 359 321 283
281 231 168 159 135 124 101 99
57 24 15 12 6 6 6
MspA1I CmG'CkG
Cuts at: 1606 4256 4614 5291 6439 6684 1606
Size: 2650 358 677 1148 245 2120
Fragments arranged by size:
2650 2120 1148 677 358 245
NcoI C'CATG_G
Cuts at: 513 1065 2095 3958 513
Size: 552 1030 1863 3753
Fragments arranged by size:
3753 1863 1030 552
NdeI CA'TA_TG
Cuts at: 387 2016 2514 387
Size: 1629 498 5071
Fragments arranged by size:
5071 1629 498
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7198
NotI GC'GGCC_GC
Cuts at: 4645 4645
Size: 7198
NsiI A_TGCA'T
Cuts at: 2520 2520
Size: 7198
NspI r_CATG'y
Cuts at: 1132 1229 2951 3425 4243 4315 4556 6101 1132
Size: 97 1722 474 818 72 241 1545 2229
Fragments arranged by size:
2229 1722 1545 818 474 241 97 72
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7198
PciI A'CATG_T
Cuts at: 2947 6097 2947
Size: 3150 4048
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7198
PmeI GTTT'AAAC
Cuts at: 4502 4502
Size: 7198
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5267 5299 6780 6812 5267
Size: 32 1481 32 5653
Fragments arranged by size:
5653 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7198
PsiI TTA'TAA
Cuts at: 4768 4768
Size: 7198
PstI C_TGCA'G
Cuts at: 830 4309 4550 830
Size: 3479 241 3478
Fragments arranged by size:
3479 3478 241
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7198
PvuII CAG'CTG
Cuts at: 4256 4256
Size: 7198
SalI G'TCGA_C
Cuts at: 1936 4540 1936
Size: 2604 4594
SbfI CC_TGCA'GG
Cuts at: 4550 4550
Size: 7198
ScaI AGT'ACT
Cuts at: 4108 5446 7158 4108
Size: 1338 1712 4148
Fragments arranged by size:
4148 1712 1338
SexAI A'CCwGG_T
Cuts at: 2749 3183 2749
Size: 434 6764
SfcI C'TryA_G
Cuts at: 826 1045 3345 4113 4305 4546 5681 6362 6553
Size: 219 2300 768 192 241 1135 681 191
Cuts at: 6553 826
Size: 1471
Fragments arranged by size:
2300 1471 1135 768 681 241 219 192
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7198
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7198
SmaI CCC'GGG
Cuts at: 1872 4525 1872
Size: 2653 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3686 5302 6203 6465 820
Size: 197 926 1743 1616 901 262 1553
Fragments arranged by size:
1743 1616 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4989 493
Size: 4496 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7198
SphI G_CATG'C
Cuts at: 1132 4243 4556 1132
Size: 3111 313 3774
Fragments arranged by size:
3774 3111 313
SspI AAT'ATT
Cuts at: 5 52 5122 5
Size: 47 5070 2081
Fragments arranged by size:
5070 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7198
StyI C'CwwG_G
Cuts at: 513 1065 2095 2275 3301 3958 4329 4637 513
Size: 552 1030 180 1026 657 371 308 3074
Fragments arranged by size:
3074 1030 1026 657 552 371 308 180
SwaI ATTT'AAAT
Cuts at: 2409 2409
Size: 7198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5225 5242 5395 5580 6025 325
Size: 1255 3645 17 153 185 445 1498
Fragments arranged by size:
3645 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2818 2842 4106
Size: 274 80 33 51 1443 841 24 1264
Cuts at: 4106 5444 7156 96
Size: 1338 1712 138
Fragments arranged by size:
1712 1443 1338 1264 841 274 138 80
51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2378 2410 7158 7190 2378
Size: 32 4748 32 2386
Fragments arranged by size:
4748 2386 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7198
XbaI T'CTAG_A
Cuts at: 4534 4534
Size: 7198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3395 3425 3672 3395
Size: 30 247 6921
Fragments arranged by size:
6921 247 30
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7198
XmaI C'CCGG_G
Cuts at: 1870 4523 1870
Size: 2653 4545
XmnI GAAnn'nnTTC
Cuts at: 2109 3637 5327 2109
Size: 1528 1690 3980
Fragments arranged by size:
3980 1690 1528
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5006 276
Size: 53 83 186 936 3472 2468
Fragments arranged by size:
3472 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BplI BsiWI
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI