(Circular) (Six-base) MAPSORT of: pFN21AA1254.seq Check: 3288 from: 1 to: 6976 pFN21AA1254 6976 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4786 278 Size: 53 83 186 936 3250 2468 Fragments arranged by size: 3250 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2435 4319 1937 Size: 498 1884 4594 Fragments arranged by size: 4594 1884 498 Acc65I G'GTAC_C Cuts at: 4297 4297 Size: 6976 AclI AA'CG_TT Cuts at: 1563 5103 5476 1563 Size: 3540 373 3063 Fragments arranged by size: 3540 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2550 2595 2892 5039 6423 6691 1632 Size: 918 45 297 2147 1384 268 1917 Fragments arranged by size: 2147 1917 1384 918 297 268 45 AflII C'TTAA_G Cuts at: 820 1017 3065 820 Size: 197 2048 4731 Fragments arranged by size: 4731 2048 197 AflIII A'CryG_T Cuts at: 1203 3993 5875 6852 1203 Size: 2790 1882 977 1327 Fragments arranged by size: 2790 1882 1327 977 AgeI A'CCGG_T Cuts at: 5807 5807 Size: 6976 AhdI GACnn_n'nnGTC Cuts at: 5705 5705 Size: 6976 AleI CACnn'nnGTG Cuts at: 1166 3944 1166 Size: 2778 4198 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6976 AlwNI CAG_nnn'CTG Cuts at: 2456 2573 4719 6291 2456 Size: 117 2146 1572 3141 Fragments arranged by size: 3141 2146 1572 117 ApaLI G'TGCA_C Cuts at: 5033 6189 5033 Size: 1156 5820 ApoI r'AATT_y Cuts at: 1493 2014 2086 3496 3575 3809 3826 3865 3968 Size: 521 72 1410 79 234 17 39 103 Cuts at: 3968 4285 4511 4663 6772 1493 Size: 317 226 152 2109 1697 Fragments arranged by size: 2109 1697 1410 521 317 234 226 152 103 79 72 39 17 AseI AT'TA_AT Cuts at: 160 1032 5530 160 Size: 872 4498 1606 Fragments arranged by size: 4498 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6976 AvaI C'yCGr_G Cuts at: 1870 1943 4301 1870 Size: 73 2358 4545 Fragments arranged by size: 4545 2358 73 BamHI G'GATC_C Cuts at: 4306 4673 4306 Size: 367 6609 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3730 4297 5752 618 Size: 325 217 627 1943 567 1455 1842 Fragments arranged by size: 1943 1842 1455 627 567 325 217 BanII G_rGCy'C Cuts at: 1391 2118 2417 4295 1391 Size: 727 299 1878 4072 Fragments arranged by size: 4072 1878 727 299 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2286 2406 2558 2583 928 Size: 971 387 120 152 25 5321 Fragments arranged by size: 5321 971 387 152 120 25 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5167 5201 1903 Size: 34 3230 34 3678 Fragments arranged by size: 3678 3230 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2950 4003 4868 6078 2950 Size: 1053 865 1210 3848 Fragments arranged by size: 3848 1210 1053 865 BclI T'GATC_A Cuts at: 1548 4036 4081 1548 Size: 2488 45 4443 Fragments arranged by size: 4443 2488 45 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2193 5587 136 Size: 107 122 71 631 605 521 3394 1525 Fragments arranged by size: 3394 1525 631 605 521 122 107 71 BglII A'GATC_T Cuts at: 6971 6971 Size: 6976 BlpI GC'TnA_GC Cuts at: 4393 4393 Size: 6976 Bme1580I G_kGCm'C Cuts at: 1790 5037 6193 1790 Size: 3247 1156 2573 Fragments arranged by size: 3247 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6976 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2511 2788 4707 Size: 416 53 83 399 24 1094 277 1919 Cuts at: 4707 5665 442 Size: 958 1753 Fragments arranged by size: 1919 1753 1094 958 416 399 277 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6976 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2966 3182 3188 5636 1239 Size: 1727 216 6 2448 2579 Fragments arranged by size: 2579 2448 1727 216 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2351 5101 5966 6264 2351 Size: 2750 865 298 3063 Fragments arranged by size: 3063 2750 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2651 5639 882 Size: 617 1152 2988 2219 Fragments arranged by size: 2988 2219 1152 617 BsaAI yAC'GTr Cuts at: 493 1204 4767 493 Size: 711 3563 2702 Fragments arranged by size: 3563 2702 711 BsaBI GATnn'nnATC Cuts at: 2681 6970 2681 Size: 4289 2687 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2536 4783 5165 5800 Size: 53 83 186 936 1003 2247 382 635 Cuts at: 5800 275 Size: 1451 Fragments arranged by size: 2247 1451 1003 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5409 5807 6081 6228 6639 5409 Size: 398 274 147 411 5746 Fragments arranged by size: 5746 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3113 3143 1178 Size: 30 165 30 1710 30 5011 Fragments arranged by size: 5011 1710 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2162 1186 Size: 976 6000 BseYI C'CCAG_C Cuts at: 6179 6675 6179 Size: 496 6480 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6403 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4426 5187 6215 1540 Size: 275 186 2425 761 1028 2301 Fragments arranged by size: 2425 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3913 5037 5122 6193 3913 Size: 1124 85 1071 4696 Fragments arranged by size: 4696 1124 1071 85 BsmI GAATG_Cn' Cuts at: 4487 4580 4487 Size: 93 6883 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2417 3913 4295 5037 5122 6193 Size: 399 328 299 1496 382 742 85 1071 Cuts at: 6193 1391 Size: 2174 Fragments arranged by size: 2174 1496 1071 742 399 382 328 299 85 BspEI T'CCGG_A Cuts at: 6639 6639 Size: 6976 BspHI T'CATG_A Cuts at: 2059 4865 6595 2059 Size: 2806 1730 2440 Fragments arranged by size: 2806 2440 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4331 844 Size: 3487 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4863 1394 Size: 429 3040 3507 Fragments arranged by size: 3507 3040 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2159 5471 5645 65 Size: 1183 911 3312 174 1396 Fragments arranged by size: 3312 1396 1183 911 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2580 5620 5807 1700 Size: 110 770 3040 187 2869 Fragments arranged by size: 3040 2869 770 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6976 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6976 BssSI C'ACGA_G Cuts at: 5036 6048 5036 Size: 1012 5964 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6976 BstBI TT'CG_AA Cuts at: 3324 5780 3324 Size: 2456 4520 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6976 BstXI CCAn_nnnn'nTGG Cuts at: 2708 2708 Size: 6976 BstYI r'GATC_y Cuts at: 1972 4306 4673 5058 5075 6527 6613 6625 6971 Size: 2334 367 385 17 1452 86 12 346 Cuts at: 6971 1972 Size: 1977 Fragments arranged by size: 2334 1977 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2436 2436 Size: 6976 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6424 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6237 Fragments arranged by size: 6237 683 56 BtsI GCAGTG_nn' Cuts at: 4492 5286 5306 4492 Size: 794 20 6162 Fragments arranged by size: 6162 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6976 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6941 DraI TTT'AAA Cuts at: 3613 3805 4280 3613 Size: 192 475 6309 Fragments arranged by size: 6309 475 192 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5983 809 Size: 507 4667 1802 Fragments arranged by size: 4667 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2698 4423 5312 Size: 54 1425 50 275 18 868 1725 889 Cuts at: 5312 6941 8 Size: 1629 43 Fragments arranged by size: 1725 1629 1425 889 868 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4423 1537 Size: 275 2611 4090 Fragments arranged by size: 4090 2611 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2422 2976 3075 4097 4906 1353 Size: 1069 554 99 1022 809 3423 Fragments arranged by size: 3423 1069 1022 809 554 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5563 5937 6083 387 Size: 1064 138 3974 374 146 1280 Fragments arranged by size: 3974 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2550 2595 2892 2966 3182 3188 5039 Size: 393 918 45 297 74 216 6 1851 Cuts at: 5039 5636 6423 6691 1239 Size: 597 787 268 1524 Fragments arranged by size: 1851 1524 918 787 597 393 297 268 216 74 45 6 EcoNI CCTnn'n_nnAGG Cuts at: 2948 2948 Size: 6976 EcoO109I rG'GnC_Cy Cuts at: 3758 3758 Size: 6976 EcoRI G'AATT_C Cuts at: 2086 3496 3809 4285 4663 2086 Size: 1410 313 476 378 4399 Fragments arranged by size: 4399 1410 476 378 313 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2168 2864 2905 3337 3649 4142 2168 Size: 696 41 432 312 493 5002 Fragments arranged by size: 5002 696 493 432 312 41 FspI TGC'GCA Cuts at: 5482 5482 Size: 6976 HaeII r_GCGC'y Cuts at: 1678 6123 1678 Size: 4445 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2471 2503 2614 2646 2816 Size: 32 135 32 899 32 111 32 170 Cuts at: 2816 2848 2870 2902 5617 5649 5691 5723 1373 Size: 32 22 32 2715 32 42 32 2626 Fragments arranged by size: 2715 2626 899 170 135 111 42 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 2735 2801 3132 4320 4566 669 Size: 988 281 797 66 331 1188 246 3079 Fragments arranged by size: 3079 1188 988 797 331 281 246 66 HindIII A'AGCT_T Cuts at: 748 748 Size: 6976 HpaI GTT'AAC Cuts at: 4566 4566 Size: 6976 KpnI G_GTAC'C Cuts at: 4301 4301 Size: 6976 MfeI C'AATT_G Cuts at: 2250 4575 2250 Size: 2325 4651 MluI A'CGCG_T Cuts at: 6852 6852 Size: 6976 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2024 2840 4158 6090 6274 1305 Size: 719 816 1318 1932 184 2007 Fragments arranged by size: 2007 1932 1318 816 719 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2700 10 Size: 54 1425 343 868 4286 Fragments arranged by size: 4286 1425 868 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2706 2760 3944 4934 5293 5452 518 Size: 648 1540 54 1184 990 359 159 2042 Fragments arranged by size: 2042 1540 1184 990 648 359 159 54 MspA1I CmG'CkG Cuts at: 1606 2109 3680 4392 5069 6217 6462 1606 Size: 503 1571 712 677 1148 245 2120 Fragments arranged by size: 2120 1571 1148 712 677 503 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6424 NdeI CA'TA_TG Cuts at: 387 4228 387 Size: 3841 3135 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6976 NotI GC'GGCC_GC Cuts at: 4423 4423 Size: 6976 NspI r_CATG'y Cuts at: 1132 1229 3997 4334 5879 1132 Size: 97 2768 337 1545 2229 Fragments arranged by size: 2768 2229 1545 337 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6976 PciI A'CATG_T Cuts at: 3993 5875 3993 Size: 1882 5094 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6976 PmeI GTTT'AAAC Cuts at: 4280 4280 Size: 6976 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5045 5077 6558 6590 5045 Size: 32 1481 32 5431 Fragments arranged by size: 5431 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 4237 1613 Size: 2624 4352 PsiI TTA'TAA Cuts at: 2829 4546 2829 Size: 1717 5259 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2256 2288 2256 Size: 32 6944 PstI C_TGCA'G Cuts at: 830 4328 830 Size: 3498 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6976 PvuII CAG'CTG Cuts at: 2109 3680 2109 Size: 1571 5405 SalI G'TCGA_C Cuts at: 1936 4318 1936 Size: 2382 4594 SapI GCTCTTCn'nnn_ Cuts at: 2422 4097 2422 Size: 1675 5301 SbfI CC_TGCA'GG Cuts at: 4328 4328 Size: 6976 ScaI AGT'ACT Cuts at: 3037 5224 6936 3037 Size: 2187 1712 3077 Fragments arranged by size: 3077 2187 1712 SfcI C'TryA_G Cuts at: 826 1045 2916 3735 4324 5459 6140 6331 826 Size: 219 1871 819 589 1135 681 191 1471 Fragments arranged by size: 1871 1471 1135 819 681 589 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6976 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6976 SmaI CCC'GGG Cuts at: 1872 4303 1872 Size: 2431 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2330 3065 5080 5981 6243 820 Size: 197 926 387 735 2015 901 262 1553 Fragments arranged by size: 2015 1553 926 901 735 387 262 197 SnaBI TAC'GTA Cuts at: 493 4767 493 Size: 4274 2702 SpeI A'CTAG_T Cuts at: 152 3175 152 Size: 3023 3953 SphI G_CATG'C Cuts at: 1132 4334 1132 Size: 3202 3774 SspI AAT'ATT Cuts at: 5 52 3015 4900 5 Size: 47 2963 1885 2081 Fragments arranged by size: 2963 2081 1885 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6976 StyI C'CwwG_G Cuts at: 513 1065 3504 4415 513 Size: 552 2439 911 3074 Fragments arranged by size: 3074 2439 911 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5003 5020 5173 5358 5803 325 Size: 1255 3423 17 153 185 445 1498 Fragments arranged by size: 3423 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3035 5222 6934 Size: 274 80 33 51 1443 1058 2187 1712 Cuts at: 6934 96 Size: 138 Fragments arranged by size: 2187 1712 1443 1058 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4202 4234 6936 6968 4202 Size: 32 2702 32 4210 Fragments arranged by size: 4210 2702 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6976 XbaI T'CTAG_A Cuts at: 4312 4312 Size: 6976 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3212 3212 Size: 6976 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6976 XmaI C'CCGG_G Cuts at: 1870 4301 1870 Size: 2431 4545 XmnI GAAnn'nnTTC Cuts at: 3575 4091 5105 3575 Size: 516 1014 5446 Fragments arranged by size: 5446 1014 516 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4784 276 Size: 53 83 186 936 3250 2468 Fragments arranged by size: 3250 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI