Restriction map for
pFN21AA1230
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
Aor51HI AscI AseI AsnI Asp718I
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaHI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII Hin1I KasI KpnI
MluI MroI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PflFI PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 2363 GTCTAC GT^[AC][GT]AC
AccII 1866 CGCG CG^CG
AclI 3028 AACGTT AA^CGTT
AgeI 2870 ACCGGT A^CCGGT
AhdI 2269 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1619 GTGCAC G^TGCAC
AocI 2334 CCTGAGG CC^T[ACGT]AGG
ApaI 1870 GGGCCC GGGCC^C
ApaLI 1619 GTGCAC G^TGCAC
AspEI 2269 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1889 CTCGAG C^[CT]CG[GA]G
BanI 1333 GGTGCC G^G[CT][GA]CC
BbvCI 2626 GCTGAGG CCTCAGC(-5/-2)
BcnI 1578 CCGGG CC^[GC]GG
BglI 1509 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 298 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1886 GGTCTC GGTCTC(1/5)
BsaWI 2870 ACCGGT [AT]^CCGG[AT]
BsgI 145 CTGCAC GTGCAG(16/14)
BsoBI 1889 CTCGAG C^[CT]CG[GA]G
BspHI 1766 TCATGA T^CATGA
BsrFI 2870 ACCGGT [GA]^CCGG[CT]
BstAPI 2464 GCAGAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1866 CGCG CG^CG
BstXI 1391 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2334 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2870 ACCGGT [GA]^CCGG[CT]
DraIII 148 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2269 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2334 CCTGAGG CC^T[ACGT]AGG
EcoNI 2847 CCTGTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1867 GCGGG CCCGC(4/6)
HgaI 2946 GACGC GACGC(5/10)
HindIII 679 AAGCTT A^AGCTT
HpaI 366 GTTAAC GTT^AAC
KspI 1865 CCGCGG CCGC^GG
MamI 298 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1959 CAATTG C^AATTG
MslI 1524 CACCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1959 CAATTG C^AATTG
MvnI 1866 CGCG CG^CG
NciI 1578 CCGGG CC^[GC]GG
NheI 2106 GCTAGC G^CTAGC
PaeR7I 1889 CTCGAG C^TCGAG
PflMI 2115 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2870 ACCGGT A^CCGGT
Psp1406I 3028 AACGTT AA^CGTT
PvuII 551 CAGCTG CAG^CTG
RcaI 1766 TCATGA T^CATGA
SacII 1865 CCGCGG CCGC^GG
SspI 80 AATATT AAT^ATT
Van91I 2115 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1889 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BseRI 1086 CTCCTC GAGGAG(10/8)
1847 GAGGAG
EcoRI 1837 GAATTC G^AATTC
1995 GAATTC
HincII 366 GTTAAC GT[CT]^[GA]AC
1278 GTTGAC
HindII 366 GTTAAC GT[CT]^[GA]AC
1278 GTTGAC
HphI 2761 GGTGA GGTGA(8/7)
1135 TCACC
PpuMI 1492 GGGACCC [GA]G^G[AT]CC[CT]
2330 AGGTCCT
SacI 140 GAGCTC GAGCT^C
1466 GAGCTC
SapI 424 GAAGAGC GCTCTTC(1/4)
1060 GAAGAGC
SexAI 374 ACCAGGT A^CC[AT]GGT
2829 ACCTGGT
SmlI 207 CTTGAG C^T[CT][GA]AG
1889 CTCGAG
XbaI 2055 TCTAGA T^CTAGA
2517 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanII BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspLU11I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI