Restriction map for pFN21AA1198
 (Circular) (Six-base) MAPSORT of: pFN21AA1198.seq  Check: 3072  from: 1  to: 6292



pFN21AA1198  6292 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:23  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4102     278 
   Size:         53      83     186     936    2566    2468

  Fragments arranged by size: 

                2566    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3517    3580    3635    1937 
   Size:       1580      63      55    4594

  Fragments arranged by size: 

                4594    1580      63      55
 
Acc65I G'GTAC_C

Cuts at:   3613    3613 
   Size:       6292
 
AclI AA'CG_TT

Cuts at:   1563    4419    4792    1563 
   Size:       2856     373    3063

  Fragments arranged by size: 

                3063    2856     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2932    3203    3361    3520    4355    5739    6007    1632 
   Size:       1300     271     158     159     835    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1300     835     271     268     159     158
            
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6095
 
AflIII A'CryG_T

Cuts at:   1203    3058    3092    5191    6168    1203 
   Size:       1855      34    2099     977    1327

  Fragments arranged by size: 

                2099    1855    1327     977      34
 
AgeI A'CCGG_T

Cuts at:   5123    5123 
   Size:       6292
 
AhdI GACnn_n'nnGTC

Cuts at:   5021    5021 
   Size:       6292
 
AleI CACnn'nnGTG

Cuts at:   1166    3413    1166 
   Size:       2247    4045
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6292
 
AlwNI CAG_nnn'CTG

Cuts at:   4035    5607    4035 
   Size:       1572    4720
 
ApaLI G'TGCA_C

Cuts at:   3140    3560    4349    5505    3140 
   Size:        420     789    1156    3927

  Fragments arranged by size: 

                3927    1156     789     420
 
ApoI r'AATT_y

Cuts at:   1493    2014    2646    2730    3463    3486    3601    3827    3979 
   Size:        521     632      84     733      23     115     226     152

Cuts at:   3979    6088    1493 
   Size:       2109    1697

  Fragments arranged by size: 

                2109    1697     733     632     521     226     152     115
                  84      23
 
AseI AT'TA_AT

Cuts at:    160    1032    4846     160 
   Size:        872    3814    1606

  Fragments arranged by size: 

                3814    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6292
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2048    3617    1870 
   Size:         73     105    1569    4545

  Fragments arranged by size: 

                4545    1569     105      73
 
BamHI G'GATC_C

Cuts at:   2227    3622    3989    2227 
   Size:       1395     367    4530

  Fragments arranged by size: 

                4530    1395     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2802    3613    5068     618 
   Size:        325     217     627    1015     811    1455    1842

  Fragments arranged by size: 

                1842    1455    1015     811     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3611    1391 
   Size:       2220    4072
 
BbeI G_GCGC'C

Cuts at:   2806    2806 
   Size:       6292
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2488    3358     928 
   Size:        971     589     870    3862

  Fragments arranged by size: 

                3862     971     870     589
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    2677    2711    4483    4517    1903 
   Size:         34      50      34     656      34    1772      34    3678

  Fragments arranged by size: 

                3678    1772     656      50      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   4184    5394    4184 
   Size:       1210    5082
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6292
 
BfrBI ATG'CAT

Cuts at:   2503    2503 
   Size:       6292
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4903     136 
   Size:        107     122      71     631     605    3231    1525

  Fragments arranged by size: 

                3231    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2531    6287    2531 
   Size:       3756    2536
 
BlpI GC'TnA_GC

Cuts at:   3709    3709 
   Size:       6292
 
Bme1580I G_kGCm'C

Cuts at:   1790    3144    3564    4353    5509    1790 
   Size:       1354     420     789    1156    2573

  Fragments arranged by size: 

                2573    1354    1156     789     420
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6292
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4023    4981     442 
   Size:        416      53      83     399      24    2606     958    1753

  Fragments arranged by size: 

                2606    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6292
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2102    2228    2608    2676    2760    2844    2928    3012 
   Size:        863     126     380      68      84      84      84      84

Cuts at:   3012    3432    3516    4952    1239 
   Size:        420      84    1436    2579

  Fragments arranged by size: 

                2579    1436     863     420     380     126      84      84
                  84      84      84      68
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2201    3028    4417    5282    5580    2201 
   Size:        827    1389     865     298    2913

  Fragments arranged by size: 

                2913    1389     865     827     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2515    4955     882 
   Size:        617    1016    2440    2219

  Fragments arranged by size: 

                2440    2219    1016     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2961    3061    3559    4083     493 
   Size:        711    1757     100     498     524    2702

  Fragments arranged by size: 

                2702    1757     711     524     498     100
 
BsaBI GATnn'nnATC

Cuts at:   6286    6286 
   Size:       6292
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2803    4099    4481    5116 
   Size:         53      83     186     936    1270    1296     382     635

Cuts at:   5116     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1296    1270     936     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3074    4725    5123    5397    5544    5955    3074 
   Size:       1651     398     274     147     411    3411

  Fragments arranged by size: 

                3411    1651     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2159    2189    1178 
   Size:         30     165      30     756      30    5281

  Fragments arranged by size: 

                5281     756     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2066    2225    1186 
   Size:        880     159    5253

  Fragments arranged by size: 

                5253     880     159
 
BseYI C'CCAG_C

Cuts at:   5495    5991    5495 
   Size:        496    5796
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5719
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3742    4503    5531    1540 
   Size:        275     186    1741     761    1028    2301

  Fragments arranged by size: 

                2301    1741    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3144    3564    4353    4438    5509    3144 
   Size:        420     789      85    1071    3927

  Fragments arranged by size: 

                3927    1071     789     420      85
 
BsmI GAATG_Cn'

Cuts at:   2777    3350    3803    3896    2777 
   Size:        573     453      93    5173

  Fragments arranged by size: 

                5173     573     453      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    3144    3564    3611    4353    4438    5509    1391 
   Size:        399    1354     420      47     742      85    1071    2174

  Fragments arranged by size: 

                2174    1354    1071     742     420     399      85      47
            
 
BspEI T'CCGG_A

Cuts at:   5955    5955 
   Size:       6292
 
BspHI T'CATG_A

Cuts at:   2974    4181    5911    2974 
   Size:       1207    1730    3355

  Fragments arranged by size: 

                3355    1730    1207
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3647     844 
   Size:       2803    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4179    1394 
   Size:        429    2356    3507

  Fragments arranged by size: 

                3507    2356     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2939    4787    4961      65 
   Size:       1183    1691    1848     174    1396

  Fragments arranged by size: 

                1848    1691    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4936    5123    1700 
   Size:        110    3126     187    2869

  Fragments arranged by size: 

                3126    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6292
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6292
 
BssSI C'ACGA_G

Cuts at:   4352    5364    4352 
   Size:       1012    5280
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6292
 
BstBI TT'CG_AA

Cuts at:   2632    2716    3052    3304    3388    5096    2632 
   Size:         84     336     252      84    1708    3828

  Fragments arranged by size: 

                3828    1708     336     252      84      84
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6292
 
BstYI r'GATC_y

Cuts at:   1972    2227    2531    3622    3989    4374    4391    5843    5929 
   Size:        255     304    1091     367     385      17    1452      86

Cuts at:   5929    5941    6287    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452    1091     385     367     346     304     255
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2140     513 
   Size:        552    1075    4665

  Fragments arranged by size: 

                4665    1075     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5553

  Fragments arranged by size: 

                5553     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2485    2790    3443    3808    4602    4622    2485 
   Size:        305     653     365     794      20    4155

  Fragments arranged by size: 

                4155     794     653     365     305      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6292
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2581    2616    3337    3372     529 
   Size:         35    2017      35     721      35    3449

  Fragments arranged by size: 

                3449    2017     721      35      35      35
 
DraI TTT'AAA

Cuts at:   3462    3596    3462 
   Size:        134    6158
 
DraIII CAC_nnn'GTG

Cuts at:   2378    2378 
   Size:       6292
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5299     809 
   Size:        507    3983    1802

  Fragments arranged by size: 

                3983    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2233    3739    4628 
   Size:         54    1425      50     275      18     403    1506     889

Cuts at:   4628    6257       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1506    1425     889     403     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3739    1537 
   Size:        275    1927    4090

  Fragments arranged by size: 

                4090    1927     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2802    4222    1353 
   Size:       1449    1420    3423

  Fragments arranged by size: 

                3423    1449    1420
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4879    5253    5399     387 
   Size:       1064     138    3290     374     146    1280

  Fragments arranged by size: 

                3290    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2102    2228    2608    2676    2760    2844    2928 
   Size:        393     470     126     380      68      84      84      84

Cuts at:   2928    2932    3012    3203    3361    3432    3516    3520    4355 
   Size:          4      80     191     158      71      84       4     835

Cuts at:   4355    4952    5739    6007    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524     835     787     597     470     393     380     268
                 191     158     126      84      84      84      84      80
                  71      68       4       4
 
EcoRI G'AATT_C

Cuts at:   2014    2646    2730    3486    3601    3979    2014 
   Size:        632      84     756     115     378    4327

  Fragments arranged by size: 

                4327     756     632     378     115      84
 
EcoRV GAT'ATC

Cuts at:   2786    2786 
   Size:       6292
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3302    3302 
   Size:       6292
 
FspI TGC'GCA

Cuts at:   2009    4798    2009 
   Size:       2789    3503
 
HaeII r_GCGC'y

Cuts at:   1678    2806    5439    1678 
   Size:       1128    2633    2531

  Fragments arranged by size: 

                2633    2531    1128
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2136    2159    2168    2191    2413 
   Size:         32     135      32     564      23       9      23     222

Cuts at:   2413    2445    4933    4965    5007    5039    1373 
   Size:         32    2488      32      42      32    2626

  Fragments arranged by size: 

                2626    2488     564     222     135      42      32      32
                  32      32      32      23      23       9
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3636    3882     669 
   Size:        988     281    1698     246    3079

  Fragments arranged by size: 

                3079    1698     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    3447     748 
   Size:       2699    3593
 
HpaI GTT'AAC

Cuts at:   3882    3882 
   Size:       6292
 
KasI G'GCGC_C

Cuts at:   2802    2802 
   Size:       6292
 
KpnI G_GTAC'C

Cuts at:   3617    3617 
   Size:       6292
 
MfeI C'AATT_G

Cuts at:   3891    3891 
   Size:       6292
 
MluI A'CGCG_T

Cuts at:   6168    6168 
   Size:       6292
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5406    5590    1305 
   Size:       4101     184    2007

  Fragments arranged by size: 

                4101    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2235      10 
   Size:         54    1425     343     403    4067

  Fragments arranged by size: 

                4067    1425     403     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2568    2587    3413    3427    4250    4609    4768 
   Size:        648    1402      19     826      14     823     359     159

Cuts at:   4768     518 
   Size:       2042

  Fragments arranged by size: 

                2042    1402     826     823     648     359     159      19
                  14
 
MspA1I CmG'CkG

Cuts at:   1606    3708    4385    5533    5778    1606 
   Size:       2102     677    1148     245    2120

  Fragments arranged by size: 

                2120    2102    1148     677     245
 
NarI GG'CG_CC

Cuts at:   2803    2803 
   Size:       6292
 
NcoI C'CATG_G

Cuts at:    513    1065    2140     513 
   Size:        552    1075    4665

  Fragments arranged by size: 

                4665    1075     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6292
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6292
 
NotI GC'GGCC_GC

Cuts at:   3739    3739 
   Size:       6292
 
NsiI A_TGCA'T

Cuts at:   2505    2505 
   Size:       6292
 
NspI r_CATG'y

Cuts at:   1132    1229    3096    3650    5195    1132 
   Size:         97    1867     554    1545    2229

  Fragments arranged by size: 

                2229    1867    1545     554      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6292
 
PciI A'CATG_T

Cuts at:   3092    5191    3092 
   Size:       2099    4193
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6292
 
PmeI GTTT'AAAC

Cuts at:   3596    3596 
   Size:       6292
 
PmlI CAC'GTG

Cuts at:   3061    3559    3061 
   Size:        498    5794
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4361    4393    5874    5906    4361 
   Size:         32    1481      32    4747

  Fragments arranged by size: 

                4747    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6292
 
PsiI TTA'TAA

Cuts at:   3862    3862 
   Size:       6292
 
PspXI vC'TCGA_Gb

Cuts at:   2048    2048 
   Size:       6292
 
PstI C_TGCA'G

Cuts at:    830    2480    3644     830 
   Size:       1650    1164    3478

  Fragments arranged by size: 

                3478    1650    1164
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6292
 
SalI G'TCGA_C

Cuts at:   1936    3634    1936 
   Size:       1698    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2802    2802 
   Size:       6292
 
SbfI CC_TGCA'GG

Cuts at:   3644    3644 
   Size:       6292
 
ScaI AGT'ACT

Cuts at:   4540    6252    4540 
   Size:       1712    4580
 
SfcI C'TryA_G

Cuts at:    826    1045    2248    2476    3640    4775    5456    5647     826 
   Size:        219    1203     228    1164    1135     681     191    1471

  Fragments arranged by size: 

                1471    1203    1164    1135     681     228     219     191
            
 
SfoI GGC'GCC

Cuts at:   2804    2804 
   Size:       6292
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6292
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6292
 
SmaI CCC'GGG

Cuts at:   1872    3619    1872 
   Size:       1747    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2048    2180    3043    4396    5297    5559 
   Size:        197     926     105     132     863    1353     901     262

Cuts at:   5559     820 
   Size:       1553

  Fragments arranged by size: 

                1553    1353     926     901     863     262     197     132
                 105
 
SnaBI TAC'GTA

Cuts at:    493    4083     493 
   Size:       3590    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6292
 
SphI G_CATG'C

Cuts at:   1132    3650    1132 
   Size:       2518    3774
 
SspI AAT'ATT

Cuts at:      5      52    4216       5 
   Size:         47    4164    2081

  Fragments arranged by size: 

                4164    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6292
 
StyI C'CwwG_G

Cuts at:    513    1065    2140    3731     513 
   Size:        552    1075    1591    3074

  Fragments arranged by size: 

                3074    1591    1075     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4319    4336    4489    4674    5119     325 
   Size:       1255    2739      17     153     185     445    1498

  Fragments arranged by size: 

                2739    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3475    4538    6250 
   Size:        274      80      33      51    1443    1498    1063    1712

Cuts at:   6250      96 
   Size:        138

  Fragments arranged by size: 

                1712    1498    1443    1063     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6252    6284    6252 
   Size:         32    6260
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6292
 
XbaI T'CTAG_A

Cuts at:   3628    3628 
   Size:       6292
 
XhoI C'TCGA_G

Cuts at:   1943    2048    1943 
   Size:        105    6187
 
XmaI C'CCGG_G

Cuts at:   1870    3617    1870 
   Size:       1747    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2609    2646    2777    2945    3234    4421    2609 
   Size:         37     131     168     289    1187    4480

  Fragments arranged by size: 

                4480    1187     289     168     131      37
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4100     276 
   Size:         53      83     186     936    2566    2468

  Fragments arranged by size: 

                2566    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NheI     NotI
     NsiI     NspI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PstI     PvuI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI
     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I  EcoICRI
    EcoNI EcoO109I     FseI    FspAI     NaeI   NgoMIV     NruI     PacI
     PfoI    PpuMI   PspOMI     PsrI    PvuII    RsrII     SacI    SacII
    SanDI    SexAI     SfiI     SrfI     SwaI     XcmI
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