(Circular) (Six-base) MAPSORT of: pFN21AA1198.seq Check: 3072 from: 1 to: 6292 pFN21AA1198 6292 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4102 278 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3517 3580 3635 1937 Size: 1580 63 55 4594 Fragments arranged by size: 4594 1580 63 55 Acc65I G'GTAC_C Cuts at: 3613 3613 Size: 6292 AclI AA'CG_TT Cuts at: 1563 4419 4792 1563 Size: 2856 373 3063 Fragments arranged by size: 3063 2856 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2932 3203 3361 3520 4355 5739 6007 1632 Size: 1300 271 158 159 835 1384 268 1917 Fragments arranged by size: 1917 1384 1300 835 271 268 159 158 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6095 AflIII A'CryG_T Cuts at: 1203 3058 3092 5191 6168 1203 Size: 1855 34 2099 977 1327 Fragments arranged by size: 2099 1855 1327 977 34 AgeI A'CCGG_T Cuts at: 5123 5123 Size: 6292 AhdI GACnn_n'nnGTC Cuts at: 5021 5021 Size: 6292 AleI CACnn'nnGTG Cuts at: 1166 3413 1166 Size: 2247 4045 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6292 AlwNI CAG_nnn'CTG Cuts at: 4035 5607 4035 Size: 1572 4720 ApaLI G'TGCA_C Cuts at: 3140 3560 4349 5505 3140 Size: 420 789 1156 3927 Fragments arranged by size: 3927 1156 789 420 ApoI r'AATT_y Cuts at: 1493 2014 2646 2730 3463 3486 3601 3827 3979 Size: 521 632 84 733 23 115 226 152 Cuts at: 3979 6088 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 733 632 521 226 152 115 84 23 AseI AT'TA_AT Cuts at: 160 1032 4846 160 Size: 872 3814 1606 Fragments arranged by size: 3814 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 AvaI C'yCGr_G Cuts at: 1870 1943 2048 3617 1870 Size: 73 105 1569 4545 Fragments arranged by size: 4545 1569 105 73 BamHI G'GATC_C Cuts at: 2227 3622 3989 2227 Size: 1395 367 4530 Fragments arranged by size: 4530 1395 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2802 3613 5068 618 Size: 325 217 627 1015 811 1455 1842 Fragments arranged by size: 1842 1455 1015 811 627 325 217 BanII G_rGCy'C Cuts at: 1391 3611 1391 Size: 2220 4072 BbeI G_GCGC'C Cuts at: 2806 2806 Size: 6292 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2488 3358 928 Size: 971 589 870 3862 Fragments arranged by size: 3862 971 870 589 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2677 2711 4483 4517 1903 Size: 34 50 34 656 34 1772 34 3678 Fragments arranged by size: 3678 1772 656 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4184 5394 4184 Size: 1210 5082 BclI T'GATC_A Cuts at: 1548 1548 Size: 6292 BfrBI ATG'CAT Cuts at: 2503 2503 Size: 6292 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4903 136 Size: 107 122 71 631 605 3231 1525 Fragments arranged by size: 3231 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2531 6287 2531 Size: 3756 2536 BlpI GC'TnA_GC Cuts at: 3709 3709 Size: 6292 Bme1580I G_kGCm'C Cuts at: 1790 3144 3564 4353 5509 1790 Size: 1354 420 789 1156 2573 Fragments arranged by size: 2573 1354 1156 789 420 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6292 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4023 4981 442 Size: 416 53 83 399 24 2606 958 1753 Fragments arranged by size: 2606 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6292 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2228 2608 2676 2760 2844 2928 3012 Size: 863 126 380 68 84 84 84 84 Cuts at: 3012 3432 3516 4952 1239 Size: 420 84 1436 2579 Fragments arranged by size: 2579 1436 863 420 380 126 84 84 84 84 84 68 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2201 3028 4417 5282 5580 2201 Size: 827 1389 865 298 2913 Fragments arranged by size: 2913 1389 865 827 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2515 4955 882 Size: 617 1016 2440 2219 Fragments arranged by size: 2440 2219 1016 617 BsaAI yAC'GTr Cuts at: 493 1204 2961 3061 3559 4083 493 Size: 711 1757 100 498 524 2702 Fragments arranged by size: 2702 1757 711 524 498 100 BsaBI GATnn'nnATC Cuts at: 6286 6286 Size: 6292 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2803 4099 4481 5116 Size: 53 83 186 936 1270 1296 382 635 Cuts at: 5116 275 Size: 1451 Fragments arranged by size: 1451 1296 1270 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3074 4725 5123 5397 5544 5955 3074 Size: 1651 398 274 147 411 3411 Fragments arranged by size: 3411 1651 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2159 2189 1178 Size: 30 165 30 756 30 5281 Fragments arranged by size: 5281 756 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2225 1186 Size: 880 159 5253 Fragments arranged by size: 5253 880 159 BseYI C'CCAG_C Cuts at: 5495 5991 5495 Size: 496 5796 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5719 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3742 4503 5531 1540 Size: 275 186 1741 761 1028 2301 Fragments arranged by size: 2301 1741 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3144 3564 4353 4438 5509 3144 Size: 420 789 85 1071 3927 Fragments arranged by size: 3927 1071 789 420 85 BsmI GAATG_Cn' Cuts at: 2777 3350 3803 3896 2777 Size: 573 453 93 5173 Fragments arranged by size: 5173 573 453 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3144 3564 3611 4353 4438 5509 1391 Size: 399 1354 420 47 742 85 1071 2174 Fragments arranged by size: 2174 1354 1071 742 420 399 85 47 BspEI T'CCGG_A Cuts at: 5955 5955 Size: 6292 BspHI T'CATG_A Cuts at: 2974 4181 5911 2974 Size: 1207 1730 3355 Fragments arranged by size: 3355 1730 1207 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3647 844 Size: 2803 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4179 1394 Size: 429 2356 3507 Fragments arranged by size: 3507 2356 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2939 4787 4961 65 Size: 1183 1691 1848 174 1396 Fragments arranged by size: 1848 1691 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4936 5123 1700 Size: 110 3126 187 2869 Fragments arranged by size: 3126 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6292 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6292 BssSI C'ACGA_G Cuts at: 4352 5364 4352 Size: 1012 5280 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6292 BstBI TT'CG_AA Cuts at: 2632 2716 3052 3304 3388 5096 2632 Size: 84 336 252 84 1708 3828 Fragments arranged by size: 3828 1708 336 252 84 84 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6292 BstYI r'GATC_y Cuts at: 1972 2227 2531 3622 3989 4374 4391 5843 5929 Size: 255 304 1091 367 385 17 1452 86 Cuts at: 5929 5941 6287 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1091 385 367 346 304 255 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2140 513 Size: 552 1075 4665 Fragments arranged by size: 4665 1075 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5553 Fragments arranged by size: 5553 683 56 BtsI GCAGTG_nn' Cuts at: 2485 2790 3443 3808 4602 4622 2485 Size: 305 653 365 794 20 4155 Fragments arranged by size: 4155 794 653 365 305 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6292 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2581 2616 3337 3372 529 Size: 35 2017 35 721 35 3449 Fragments arranged by size: 3449 2017 721 35 35 35 DraI TTT'AAA Cuts at: 3462 3596 3462 Size: 134 6158 DraIII CAC_nnn'GTG Cuts at: 2378 2378 Size: 6292 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5299 809 Size: 507 3983 1802 Fragments arranged by size: 3983 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2233 3739 4628 Size: 54 1425 50 275 18 403 1506 889 Cuts at: 4628 6257 8 Size: 1629 43 Fragments arranged by size: 1629 1506 1425 889 403 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3739 1537 Size: 275 1927 4090 Fragments arranged by size: 4090 1927 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2802 4222 1353 Size: 1449 1420 3423 Fragments arranged by size: 3423 1449 1420 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4879 5253 5399 387 Size: 1064 138 3290 374 146 1280 Fragments arranged by size: 3290 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2228 2608 2676 2760 2844 2928 Size: 393 470 126 380 68 84 84 84 Cuts at: 2928 2932 3012 3203 3361 3432 3516 3520 4355 Size: 4 80 191 158 71 84 4 835 Cuts at: 4355 4952 5739 6007 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 835 787 597 470 393 380 268 191 158 126 84 84 84 84 80 71 68 4 4 EcoRI G'AATT_C Cuts at: 2014 2646 2730 3486 3601 3979 2014 Size: 632 84 756 115 378 4327 Fragments arranged by size: 4327 756 632 378 115 84 EcoRV GAT'ATC Cuts at: 2786 2786 Size: 6292 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3302 3302 Size: 6292 FspI TGC'GCA Cuts at: 2009 4798 2009 Size: 2789 3503 HaeII r_GCGC'y Cuts at: 1678 2806 5439 1678 Size: 1128 2633 2531 Fragments arranged by size: 2633 2531 1128 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2136 2159 2168 2191 2413 Size: 32 135 32 564 23 9 23 222 Cuts at: 2413 2445 4933 4965 5007 5039 1373 Size: 32 2488 32 42 32 2626 Fragments arranged by size: 2626 2488 564 222 135 42 32 32 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 669 1657 1938 3636 3882 669 Size: 988 281 1698 246 3079 Fragments arranged by size: 3079 1698 988 281 246 HindIII A'AGCT_T Cuts at: 748 3447 748 Size: 2699 3593 HpaI GTT'AAC Cuts at: 3882 3882 Size: 6292 KasI G'GCGC_C Cuts at: 2802 2802 Size: 6292 KpnI G_GTAC'C Cuts at: 3617 3617 Size: 6292 MfeI C'AATT_G Cuts at: 3891 3891 Size: 6292 MluI A'CGCG_T Cuts at: 6168 6168 Size: 6292 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5406 5590 1305 Size: 4101 184 2007 Fragments arranged by size: 4101 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2235 10 Size: 54 1425 343 403 4067 Fragments arranged by size: 4067 1425 403 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2568 2587 3413 3427 4250 4609 4768 Size: 648 1402 19 826 14 823 359 159 Cuts at: 4768 518 Size: 2042 Fragments arranged by size: 2042 1402 826 823 648 359 159 19 14 MspA1I CmG'CkG Cuts at: 1606 3708 4385 5533 5778 1606 Size: 2102 677 1148 245 2120 Fragments arranged by size: 2120 2102 1148 677 245 NarI GG'CG_CC Cuts at: 2803 2803 Size: 6292 NcoI C'CATG_G Cuts at: 513 1065 2140 513 Size: 552 1075 4665 Fragments arranged by size: 4665 1075 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6292 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6292 NotI GC'GGCC_GC Cuts at: 3739 3739 Size: 6292 NsiI A_TGCA'T Cuts at: 2505 2505 Size: 6292 NspI r_CATG'y Cuts at: 1132 1229 3096 3650 5195 1132 Size: 97 1867 554 1545 2229 Fragments arranged by size: 2229 1867 1545 554 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6292 PciI A'CATG_T Cuts at: 3092 5191 3092 Size: 2099 4193 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6292 PmeI GTTT'AAAC Cuts at: 3596 3596 Size: 6292 PmlI CAC'GTG Cuts at: 3061 3559 3061 Size: 498 5794 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4361 4393 5874 5906 4361 Size: 32 1481 32 4747 Fragments arranged by size: 4747 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6292 PsiI TTA'TAA Cuts at: 3862 3862 Size: 6292 PspXI vC'TCGA_Gb Cuts at: 2048 2048 Size: 6292 PstI C_TGCA'G Cuts at: 830 2480 3644 830 Size: 1650 1164 3478 Fragments arranged by size: 3478 1650 1164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6292 SalI G'TCGA_C Cuts at: 1936 3634 1936 Size: 1698 4594 SapI GCTCTTCn'nnn_ Cuts at: 2802 2802 Size: 6292 SbfI CC_TGCA'GG Cuts at: 3644 3644 Size: 6292 ScaI AGT'ACT Cuts at: 4540 6252 4540 Size: 1712 4580 SfcI C'TryA_G Cuts at: 826 1045 2248 2476 3640 4775 5456 5647 826 Size: 219 1203 228 1164 1135 681 191 1471 Fragments arranged by size: 1471 1203 1164 1135 681 228 219 191 SfoI GGC'GCC Cuts at: 2804 2804 Size: 6292 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6292 SmaI CCC'GGG Cuts at: 1872 3619 1872 Size: 1747 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2048 2180 3043 4396 5297 5559 Size: 197 926 105 132 863 1353 901 262 Cuts at: 5559 820 Size: 1553 Fragments arranged by size: 1553 1353 926 901 863 262 197 132 105 SnaBI TAC'GTA Cuts at: 493 4083 493 Size: 3590 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6292 SphI G_CATG'C Cuts at: 1132 3650 1132 Size: 2518 3774 SspI AAT'ATT Cuts at: 5 52 4216 5 Size: 47 4164 2081 Fragments arranged by size: 4164 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6292 StyI C'CwwG_G Cuts at: 513 1065 2140 3731 513 Size: 552 1075 1591 3074 Fragments arranged by size: 3074 1591 1075 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4319 4336 4489 4674 5119 325 Size: 1255 2739 17 153 185 445 1498 Fragments arranged by size: 2739 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3475 4538 6250 Size: 274 80 33 51 1443 1498 1063 1712 Cuts at: 6250 96 Size: 138 Fragments arranged by size: 1712 1498 1443 1063 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6252 6284 6252 Size: 32 6260 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6292 XbaI T'CTAG_A Cuts at: 3628 3628 Size: 6292 XhoI C'TCGA_G Cuts at: 1943 2048 1943 Size: 105 6187 XmaI C'CCGG_G Cuts at: 1870 3617 1870 Size: 1747 4545 XmnI GAAnn'nnTTC Cuts at: 2609 2646 2777 2945 3234 4421 2609 Size: 37 131 168 289 1187 4480 Fragments arranged by size: 4480 1187 289 168 131 37 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4100 276 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI NaeI NgoMIV NruI PacI PfoI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI