Restriction map for
pFN21AA1179
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# NO CUT ENZYMES ( 55 enzymes) #
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AatII AccIII AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AspEI
BcgI BclI BfrI BsaBI BseAI
BsiEI BsiWI BsmBI BspEI BssHII
Bst1107I BstZ17I CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FbaI FseI HindIII KspI MamI
MluI MroI NdeI NotI NruI
PacI PinAI PmeI PshAI PvuI
RsrII SacII SalI ScaI SgrAI
SnaBI SpeI SphI SspI SwaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 520 GGTACC G^GTACC
AccII 4628 CGCG CG^CG
AclI 1097 AACGTT AA^CGTT
AcyI 5002 GGCGCC G[GA]^CG[CT]C
ApaI 3674 GGGCCC GGGCC^C
Asp718I 520 GGTACC G^GTACC
AviII 3737 TGCGCA TGC^GCA
AvrII 1086 CCTAGG C^CTAGG
BamHI 803 GGATCC G^GATCC
BbeI 5002 GGCGCC GGCGC^C
BbrPI 3265 CACGTG CAC^GTG
BbsI 4165 GAAGAC GAAGAC(2/6)
BlnI 1086 CCTAGG C^CTAGG
BpuAI 4165 GAAGAC GAAGAC(2/6)
BsaHI 5002 GGCGCC G[GA]^CG[CT]C
BspDI 4093 ATCGAT AT^CGAT
BsrFI 360 GCCGGC [GA]^CCGG[CT]
BssSI 2749 CTCGTG CACGAG(-5/-1)
BstBI 3472 TTCGAA TT^CGAA
BstEII 54 GGTGACC G^GT[ACGT]ACC
BstPI 54 GGTGACC G^GT[ACGT]ACC
BstUI 4628 CGCG CG^CG
Cfr10I 360 GCCGGC [GA]^CCGG[CT]
ClaI 4093 ATCGAT AT^CGAT
EcoO65I 54 GGTGACC G^GT[ACGT]ACC
FspI 3737 TGCGCA TGC^GCA
HaeII 5002 GGCGCC [GA]GCGC^[CT]
Hin1I 5002 GGCGCC G[GA]^CG[CT]C
HpaI 580 GTTAAC GTT^AAC
KasI 5002 GGCGCC G^GCGCC
KpnI 520 GGTACC GGTAC^C
MvnI 4628 CGCG CG^CG
NaeI 360 GCCGGC GCC^GGC
NarI 5002 GGCGCC GG^CGCC
NgoMIV 360 GCCGGC G^CCGGC
NspV 3472 TTCGAA TT^CGAA
PaeR7I 3157 CTCGAG C^TCGAG
PmaCI 3265 CACGTG CAC^GTG
PmlI 3265 CACGTG CAC^GTG
Psp1406I 1097 AACGTT AA^CGTT
SapI 192 GAAGAGC GCTCTTC(1/4)
SbfI 2772 CCTGCAGG CCTGCA^GG
SfiI 3618 GGCCCGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5002 GGCGCC GGC^GCC
SfuI 3472 TTCGAA TT^CGAA
Sse8387I 2772 CCTGCAGG CCTGCA^GG
XbaI 4833 TCTAGA T^CTAGA
XhoI 3157 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 274 GTCTAC GT^[AC][GT]AC
4717 GTAGAC
AseI 1333 ATTAAT AT^TAAT
2620 ATTAAT
AsnI 1333 ATTAAT AT^TAAT
2620 ATTAAT
BalI 3230 TGGCCA TGG^CCA
5150 TGGCCA
BbvCI 2791 GCTGAGG CCTCAGC(-5/-2)
3571 GCTGAGG
BglI 3619 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5005 GCCTACGAGGC
BglII 2709 AGATCT A^GATCT
3075 AGATCT
BsaAI 460 TACGTG [CT]AC^GT[GA]
3265 CACGTG
BspHI 1304 TCATGA T^CATGA
2513 TCATGA
BspLU11I 2837 ACATGT A^CATGT
3188 ACATGT
DraIII 914 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
2587 CACCTGGTG
DrdI 109 GACCGAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
301 GACAAGAAAGTC
HgaI 3730 GACGC GACGC(5/10)
4695 GCGTC
MfeI 2657 CAATTG C^AATTG
3366 CAATTG
MluNI 3230 TGGCCA TGG^CCA
5150 TGGCCA
MscI 3230 TGGCCA TGG^CCA
5150 TGGCCA
MunI 2657 CAATTG C^AATTG
3366 CAATTG
NcoI 2132 CCATGG C^CATGG
4958 CCATGG
NheI 2298 GCTAGC G^CTAGC
2376 GCTAGC
PshBI 1333 ATTAAT AT^TAAT
2620 ATTAAT
RcaI 1304 TCATGA T^CATGA
2513 TCATGA
SexAI 2588 ACCTGGT A^CC[AT]GGT
4547 ACCTGGT
SmaI 2012 CCCGGG CCC^GGG
3620 CCCGGG
XmaCI 2012 CCCGGG C^CCGGG
3620 CCCGGG
XmaI 2012 CCCGGG C^CCGGG
3620 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AspI AvaI AvaII BanI
BanII BbvI BciVI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmnI