(Circular) (Six-base) MAPSORT of: pFN21AA1179.seq Check: 1096 from: 1 to: 9952 pFN21AA1179 9952 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4262 4439 4262 Size: 177 9775 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7762 278 Size: 53 83 186 936 6226 2468 Fragments arranged by size: 6226 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2280 6723 7295 1937 Size: 343 4443 572 4594 Fragments arranged by size: 4594 4443 572 343 Acc65I G'GTAC_C Cuts at: 2525 7273 2525 Size: 4748 5204 AclI AA'CG_TT Cuts at: 1563 3103 8079 8452 1563 Size: 1540 4976 373 3063 Fragments arranged by size: 4976 3063 1540 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2418 3493 4209 5148 5508 8015 9399 9667 Size: 786 1075 716 939 360 2507 1384 268 Cuts at: 9667 1632 Size: 1917 Fragments arranged by size: 2507 1917 1384 1075 939 786 716 360 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9755 AflIII A'CryG_T Cuts at: 1203 4842 5193 5269 8851 9828 1203 Size: 3639 351 76 3582 977 1327 Fragments arranged by size: 3639 3582 1327 977 351 76 AgeI A'CCGG_T Cuts at: 8783 8783 Size: 9952 AhdI GACnn_n'nnGTC Cuts at: 8681 8681 Size: 9952 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9952 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4632 5389 1184 Size: 3448 757 5747 Fragments arranged by size: 5747 3448 757 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2714 2746 4721 4753 2714 Size: 32 1975 32 7913 Fragments arranged by size: 7913 1975 32 32 AlwNI CAG_nnn'CTG Cuts at: 4243 6976 6988 7695 9267 4243 Size: 2733 12 707 1572 4928 Fragments arranged by size: 4928 2733 1572 707 12 ApaI G_GGCC'C Cuts at: 5683 5683 Size: 9952 ApaLI G'TGCA_C Cuts at: 8009 9165 8009 Size: 1156 8796 ApoI r'AATT_y Cuts at: 1493 2093 2159 2502 3305 5384 5474 5495 7100 Size: 600 66 343 803 2079 90 21 1605 Cuts at: 7100 7145 7199 7261 7487 7639 9748 1493 Size: 45 54 62 226 152 2109 1697 Fragments arranged by size: 2109 2079 1697 1605 803 600 343 226 152 90 66 62 54 45 21 AseI AT'TA_AT Cuts at: 160 1032 3339 4626 8506 160 Size: 872 2307 1287 3880 1606 Fragments arranged by size: 3880 2307 1606 1287 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9952 AvaI C'yCGr_G Cuts at: 1870 1943 3039 4017 4368 5162 5429 5625 6993 Size: 73 1096 978 351 794 267 196 1368 Cuts at: 6993 7277 1870 Size: 284 4545 Fragments arranged by size: 4545 1368 1096 978 794 351 284 267 196 73 AvrII C'CTAG_G Cuts at: 3091 3091 Size: 9952 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2517 2550 2727 2760 2517 Size: 33 177 33 9709 Fragments arranged by size: 9709 177 33 33 BamHI G'GATC_C Cuts at: 2808 7282 7649 2808 Size: 4474 367 5111 Fragments arranged by size: 5111 4474 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2525 3443 3664 4200 4679 Size: 325 217 627 738 918 221 536 479 Cuts at: 4679 6889 7007 7273 8728 618 Size: 2210 118 266 1455 1842 Fragments arranged by size: 2210 1842 1455 918 738 627 536 479 325 266 221 217 118 BanII G_rGCy'C Cuts at: 1391 2039 4338 4806 5683 6066 6418 6476 7054 Size: 648 2299 468 877 383 352 58 578 Cuts at: 7054 7227 7271 1391 Size: 173 44 4072 Fragments arranged by size: 4072 2299 877 648 578 468 383 352 173 58 44 BbeI G_GCGC'C Cuts at: 7011 7011 Size: 9952 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 6177 928 Size: 971 4278 4703 Fragments arranged by size: 4703 4278 971 BbvCI CC'TCA_GC Cuts at: 4797 5577 4797 Size: 780 9172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 8143 8177 1903 Size: 34 50 34 6122 34 3678 Fragments arranged by size: 6122 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4024 6862 7067 7844 9054 4024 Size: 2838 205 777 1210 4922 Fragments arranged by size: 4922 2838 1210 777 205 BclI T'GATC_A Cuts at: 1548 1548 Size: 9952 BfrBI ATG'CAT Cuts at: 3231 4772 4896 3231 Size: 1541 124 8287 Fragments arranged by size: 8287 1541 124 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5630 7016 8563 Size: 107 122 71 631 605 3958 1386 1547 Cuts at: 8563 136 Size: 1525 Fragments arranged by size: 3958 1547 1525 1386 631 605 122 107 71 BglII A'GATC_T Cuts at: 4714 5080 9947 4714 Size: 366 4867 4719 Fragments arranged by size: 4867 4719 366 BlpI GC'TnA_GC Cuts at: 2033 5565 6903 7369 2033 Size: 3532 1338 466 4616 Fragments arranged by size: 4616 3532 1338 466 Bme1580I G_kGCm'C Cuts at: 1790 2918 4203 4684 5683 5766 8013 9169 1790 Size: 1128 1285 481 999 83 2247 1156 2573 Fragments arranged by size: 2573 2247 1285 1156 1128 999 481 83 BmgBI CAC'GTC Cuts at: 1318 3613 4064 7192 1318 Size: 2295 451 3128 4078 Fragments arranged by size: 4078 3128 2295 451 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4547 5869 6955 Size: 416 53 83 399 24 3130 1322 1086 Cuts at: 6955 7172 7683 8641 442 Size: 217 511 958 1753 Fragments arranged by size: 3130 1753 1322 1086 958 511 416 399 217 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4307 4385 1056 Size: 3251 78 6623 Fragments arranged by size: 6623 3251 78 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3065 5754 6394 6548 6592 3065 Size: 2689 640 154 44 6425 Fragments arranged by size: 6425 2689 640 154 44 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2583 3734 4232 4511 5141 5480 5795 5892 Size: 1344 1151 498 279 630 339 315 97 Cuts at: 5892 6564 6998 8612 1239 Size: 672 434 1614 2579 Fragments arranged by size: 2579 1614 1344 1151 672 630 498 434 339 315 279 97 Bpu10I CC'TnA_GC Cuts at: 4797 5577 6147 4797 Size: 780 570 8602 Fragments arranged by size: 8602 780 570 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3035 3916 5229 5399 6188 8077 8942 9240 3035 Size: 881 1313 170 789 1889 865 298 3747 Fragments arranged by size: 3747 1889 1313 881 865 789 298 170 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3983 4894 5680 8615 882 Size: 617 2484 911 786 2935 2219 Fragments arranged by size: 2935 2484 2219 911 786 617 BsaAI yAC'GTr Cuts at: 493 1204 2467 5272 7743 493 Size: 711 1263 2805 2471 2702 Fragments arranged by size: 2805 2702 2471 1263 711 BsaBI GATnn'nnATC Cuts at: 9946 9946 Size: 9952 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7008 7759 8141 8776 Size: 53 83 186 936 5475 751 382 635 Cuts at: 8776 275 Size: 1451 Fragments arranged by size: 5475 1451 936 751 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3516 4725 6916 8385 8783 9057 9204 9615 3516 Size: 1209 2191 1469 398 274 147 411 3853 Fragments arranged by size: 3853 2191 1469 1209 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2714 2744 3486 3516 3566 Size: 30 165 30 1311 30 742 30 50 Cuts at: 3566 3596 5135 5165 5360 5390 7004 7034 1178 Size: 30 1539 30 195 30 1614 30 4096 Fragments arranged by size: 4096 1614 1539 1311 742 195 165 50 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3494 4145 4277 4589 4814 5075 5316 5654 Size: 2308 651 132 312 225 261 241 338 Cuts at: 5654 6134 6605 1186 Size: 480 471 4533 Fragments arranged by size: 4533 2308 651 480 471 338 312 261 241 225 132 BseYI C'CCAG_C Cuts at: 3139 4204 4331 5332 5765 6024 6510 6708 6768 Size: 1065 127 1001 433 259 486 198 60 Cuts at: 6768 6898 7229 9155 9651 3139 Size: 130 331 1926 496 3440 Fragments arranged by size: 3440 1926 1065 1001 496 486 433 331 259 198 130 127 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2089 4619 6072 6314 1163 Size: 573 353 2530 1453 242 4801 Fragments arranged by size: 4801 2530 1453 573 353 242 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7402 8163 9191 1540 Size: 275 186 5401 761 1028 2301 Fragments arranged by size: 5401 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3051 4063 4652 4806 6066 6476 7040 8013 8098 Size: 1012 589 154 1260 410 564 973 85 Cuts at: 8098 9169 3051 Size: 1071 3834 Fragments arranged by size: 3834 1260 1071 1012 973 589 564 410 154 85 BsmI GAATG_Cn' Cuts at: 4126 4514 6782 7463 7556 4126 Size: 388 2268 681 93 6522 Fragments arranged by size: 6522 2268 681 388 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2918 3051 4063 4203 4338 4652 Size: 399 249 879 133 1012 140 135 314 Cuts at: 4652 4684 4806 5683 5766 6066 6418 6476 7040 Size: 32 122 877 83 300 352 58 564 Cuts at: 7040 7054 7227 7271 8013 8098 9169 1391 Size: 14 173 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 1012 879 877 742 564 399 352 314 300 249 173 140 135 133 122 85 83 58 44 32 14 BspEI T'CCGG_A Cuts at: 9615 9615 Size: 9952 BspHI T'CATG_A Cuts at: 3309 4518 7841 9571 3309 Size: 1209 3323 1730 3690 Fragments arranged by size: 3690 3323 1730 1209 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2072 4262 4439 6973 7307 844 Size: 1228 2190 177 2534 334 3489 Fragments arranged by size: 3489 2534 2190 1228 334 177 BsrBI CCG'CTC Cuts at: 1394 1823 2725 3718 5800 7839 1394 Size: 429 902 993 2082 2039 3507 Fragments arranged by size: 3507 2082 2039 993 902 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2366 3202 3888 3930 6747 8447 8621 Size: 1183 1118 836 686 42 2817 1700 174 Cuts at: 8621 65 Size: 1396 Fragments arranged by size: 2817 1700 1396 1183 1118 836 686 174 42 BsrFI r'CCGG_y Cuts at: 1700 1810 2365 8596 8783 1700 Size: 110 555 6231 187 2869 Fragments arranged by size: 6231 2869 555 187 110 BsrGI T'GTAC_A Cuts at: 96 4845 5022 6403 96 Size: 4749 177 1381 3645 Fragments arranged by size: 4749 3645 1381 177 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9952 BssSI C'ACGA_G Cuts at: 4754 8012 9024 4754 Size: 3258 1012 5682 Fragments arranged by size: 5682 3258 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 6101 6749 6988 794 Size: 5307 648 239 3758 Fragments arranged by size: 5307 3758 648 239 BstBI TT'CG_AA Cuts at: 5478 8756 5478 Size: 3278 6674 BstEII G'GTnAC_C Cuts at: 1184 2059 1184 Size: 875 9077 BstXI CCAn_nnnn'nTGG Cuts at: 4298 5773 5830 6032 4298 Size: 1475 57 202 8218 Fragments arranged by size: 8218 1475 202 57 BstYI r'GATC_y Cuts at: 1972 2808 3185 3418 4714 5080 6157 6453 7282 Size: 836 377 233 1296 366 1077 296 829 Cuts at: 7282 7649 8034 8051 9503 9589 9601 9947 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1296 1077 836 829 385 377 367 366 346 296 233 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2021 2040 2718 2021 Size: 19 678 9255 Fragments arranged by size: 9255 678 19 BtgI C'CryG_G Cuts at: 513 1065 4137 5889 6669 6963 513 Size: 552 3072 1752 780 294 3502 Fragments arranged by size: 3502 3072 1752 780 552 294 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4901 487 Size: 683 56 3675 5538 Fragments arranged by size: 5538 3675 683 56 BtsI GCAGTG_nn' Cuts at: 2871 3131 3599 4410 4731 7468 8262 8282 2871 Size: 260 468 811 321 2737 794 20 4541 Fragments arranged by size: 4541 2737 811 794 468 321 260 20 ClaI AT'CG_AT Cuts at: 1554 6099 1554 Size: 4545 5407 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2227 2262 529 Size: 35 1663 35 8219 Fragments arranged by size: 8219 1663 35 35 DraI TTT'AAA Cuts at: 7256 7256 Size: 9952 DraIII CAC_nnn'GTG Cuts at: 2924 4597 2924 Size: 1673 8279 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2120 2312 8959 809 Size: 507 804 192 6647 1802 Fragments arranged by size: 6647 1802 804 507 192 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2363 5235 7109 Size: 54 1425 50 275 18 533 2872 1874 Cuts at: 7109 7155 7399 8288 9917 8 Size: 46 244 889 1629 43 Fragments arranged by size: 2872 1874 1629 1425 889 533 275 244 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7399 1537 Size: 275 5587 4090 Fragments arranged by size: 5587 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2192 2826 3072 3512 5214 5475 6539 6927 Size: 839 634 246 440 1702 261 1064 388 Cuts at: 6927 7882 1353 Size: 955 3423 Fragments arranged by size: 3423 1702 1064 955 839 634 440 388 261 246 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4044 5045 5951 8539 8913 9059 Size: 1064 138 2455 1001 906 2588 374 146 Cuts at: 9059 387 Size: 1280 Fragments arranged by size: 2588 2455 1280 1064 1001 906 374 146 138 EcoICRI GAG'CTC Cuts at: 4804 6064 6474 4804 Size: 1260 410 8282 Fragments arranged by size: 8282 1260 410 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2418 2583 3493 3734 4209 4232 4511 Size: 393 786 165 910 241 475 23 279 Cuts at: 4511 5141 5148 5480 5508 5795 5892 6564 6998 Size: 630 7 332 28 287 97 672 434 Cuts at: 6998 8015 8612 9399 9667 1239 Size: 1017 597 787 268 1524 Fragments arranged by size: 1524 1017 910 787 786 672 630 597 475 434 393 332 287 279 268 241 165 97 28 23 7 EcoNI CCTnn'n_nnAGG Cuts at: 3095 4424 6086 3095 Size: 1329 1662 6961 Fragments arranged by size: 6961 1662 1329 EcoO109I rG'GnC_Cy Cuts at: 2025 2999 3145 3598 3787 4486 5631 6020 2025 Size: 974 146 453 189 699 1145 389 5957 Fragments arranged by size: 5957 1145 974 699 453 389 189 146 EcoRI G'AATT_C Cuts at: 2502 3305 5495 7261 7639 2502 Size: 803 2190 1766 378 4815 Fragments arranged by size: 4815 2190 1766 803 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3226 3523 3226 Size: 297 9655 FspI TGC'GCA Cuts at: 5744 8458 5744 Size: 2714 7238 HaeII r_GCGC'y Cuts at: 1678 7011 9099 1678 Size: 5333 2088 2531 Fragments arranged by size: 5333 2531 2088 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2147 2179 2543 2575 3486 Size: 32 135 32 575 32 364 32 911 Cuts at: 3486 3518 3991 3998 4023 4030 4459 4491 5360 Size: 32 473 7 25 7 429 32 869 Cuts at: 5360 5392 8593 8625 8667 8699 1373 Size: 32 3201 32 42 32 2626 Fragments arranged by size: 3201 2626 911 869 575 473 429 364 135 42 32 32 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2587 2988 5356 7296 7542 669 Size: 988 281 649 401 2368 1940 246 3079 Fragments arranged by size: 3079 2368 1940 988 649 401 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9952 HpaI GTT'AAC Cuts at: 2587 7542 2587 Size: 4955 4997 KasI G'GCGC_C Cuts at: 7007 7007 Size: 9952 KpnI G_GTAC'C Cuts at: 2529 7277 2529 Size: 4748 5204 MfeI C'AATT_G Cuts at: 4662 5371 7551 4662 Size: 709 2180 7063 Fragments arranged by size: 7063 2180 709 MluI A'CGCG_T Cuts at: 9828 9828 Size: 9952 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 9066 9250 1305 Size: 7761 184 2007 Fragments arranged by size: 7761 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5237 7157 10 Size: 54 1425 343 3405 1920 2805 Fragments arranged by size: 3405 2805 1920 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2520 3537 4142 4841 5018 6336 6464 Size: 648 1354 1017 605 699 177 1318 128 Cuts at: 6464 6624 7910 8269 8428 518 Size: 160 1286 359 159 2042 Fragments arranged by size: 2042 1354 1318 1286 1017 699 648 605 359 177 160 159 128 MspA1I CmG'CkG Cuts at: 1606 2177 2741 5773 5928 5960 6028 6056 6661 Size: 571 564 3032 155 32 68 28 605 Cuts at: 6661 6973 7368 8045 9193 9438 1606 Size: 312 395 677 1148 245 2120 Fragments arranged by size: 3032 2120 1148 677 605 571 564 395 312 245 155 68 32 28 NaeI GCC'GGC Cuts at: 2367 2367 Size: 9952 NarI GG'CG_CC Cuts at: 7008 7008 Size: 9952 NcoI C'CATG_G Cuts at: 513 1065 4137 6963 513 Size: 552 3072 2826 3502 Fragments arranged by size: 3502 3072 2826 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9952 NgoMIV G'CCGG_C Cuts at: 2365 2365 Size: 9952 NheI G'CTAG_C Cuts at: 1052 4303 4381 1052 Size: 3251 78 6623 Fragments arranged by size: 6623 3251 78 NotI GC'GGCC_GC Cuts at: 7399 7399 Size: 9952 NsiI A_TGCA'T Cuts at: 3233 4774 4898 3233 Size: 1541 124 8287 Fragments arranged by size: 8287 1541 124 NspI r_CATG'y Cuts at: 1132 1229 2689 4147 4846 5197 6914 7310 8855 Size: 97 1460 1458 699 351 1717 396 1545 Cuts at: 8855 1132 Size: 2229 Fragments arranged by size: 2229 1717 1545 1460 1458 699 396 351 97 PasI CC'CwG_GG Cuts at: 1348 3286 4675 1348 Size: 1938 1389 6625 Fragments arranged by size: 6625 1938 1389 PciI A'CATG_T Cuts at: 4842 5193 8851 4842 Size: 351 3658 5943 Fragments arranged by size: 5943 3658 351 PflMI CCAn_nnn'nTGG Cuts at: 1108 4882 4945 6391 6976 1108 Size: 3774 63 1446 585 4084 Fragments arranged by size: 4084 3774 1446 585 63 PfoI T'CCnGG_A Cuts at: 5340 5457 6366 6478 5340 Size: 117 909 112 8814 Fragments arranged by size: 8814 909 117 112 PmeI GTTT'AAAC Cuts at: 7256 7256 Size: 9952 PmlI CAC'GTG Cuts at: 5272 5272 Size: 9952 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2714 2746 6921 6953 8021 8053 9534 9566 2714 Size: 32 4175 32 1068 32 1481 32 3100 Fragments arranged by size: 4175 3100 1481 1068 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2025 2999 3145 3598 6020 2025 Size: 974 146 453 2422 5957 Fragments arranged by size: 5957 2422 974 453 146 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9952 PsiI TTA'TAA Cuts at: 5712 6192 7522 5712 Size: 480 1330 8142 Fragments arranged by size: 8142 1330 480 PspOMI G'GGCC_C Cuts at: 5679 5679 Size: 9952 PspXI vC'TCGA_Gb Cuts at: 5162 5162 Size: 9952 PstI C_TGCA'G Cuts at: 830 2644 4417 4782 5314 5368 5787 6061 6643 Size: 1814 1773 365 532 54 419 274 582 Cuts at: 6643 7304 830 Size: 661 3478 Fragments arranged by size: 3478 1814 1773 661 582 532 419 365 274 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9952 PvuII CAG'CTG Cuts at: 2177 2741 5773 5960 6028 6056 6661 2177 Size: 564 3032 187 68 28 605 5468 Fragments arranged by size: 5468 3032 605 564 187 68 28 SacI G_AGCT'C Cuts at: 4806 6066 6476 4806 Size: 1260 410 8282 Fragments arranged by size: 8282 1260 410 SalI G'TCGA_C Cuts at: 1936 7294 1936 Size: 5358 4594 SanDI GG'GwC_CC Cuts at: 3598 3598 Size: 9952 SapI GCTCTTCn'nnn_ Cuts at: 2192 2192 Size: 9952 SbfI CC_TGCA'GG Cuts at: 4782 7304 4782 Size: 2522 7430 ScaI AGT'ACT Cuts at: 8200 9912 8200 Size: 1712 8240 SexAI A'CCwGG_T Cuts at: 4593 6552 4593 Size: 1959 7993 SfcI C'TryA_G Cuts at: 826 1045 2640 3411 4280 4413 4718 4778 5138 Size: 219 1595 771 869 133 305 60 360 Cuts at: 5138 5227 5310 5364 5783 6015 6057 6639 6720 Size: 89 83 54 419 232 42 582 81 Cuts at: 6720 7300 8435 9116 9307 826 Size: 580 1135 681 191 1471 Fragments arranged by size: 1595 1471 1135 869 771 681 582 580 419 360 305 232 219 191 133 89 83 81 60 54 42 SfiI GGCCn_nnn'nGGCC Cuts at: 5630 5630 Size: 9952 SfoI GGC'GCC Cuts at: 7009 7009 Size: 9952 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9952 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9952 SmaI CCC'GGG Cuts at: 1872 4019 5627 7279 1872 Size: 2147 1608 1652 4545 Fragments arranged by size: 4545 2147 1652 1608 SmlI C'TyrA_G Cuts at: 820 1017 1943 3050 3895 5162 5244 5414 6203 Size: 197 926 1107 845 1267 82 170 789 Cuts at: 6203 8056 8957 9219 820 Size: 1853 901 262 1553 Fragments arranged by size: 1853 1553 1267 1107 926 901 845 789 262 197 170 82 SnaBI TAC'GTA Cuts at: 493 7743 493 Size: 7250 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9952 SphI G_CATG'C Cuts at: 1132 7310 1132 Size: 6178 3774 SspI AAT'ATT Cuts at: 5 52 7876 5 Size: 47 7824 2081 Fragments arranged by size: 7824 2081 47 StuI AGG'CCT Cuts at: 1517 5225 5694 6500 7019 1517 Size: 3708 469 806 519 4450 Fragments arranged by size: 4450 3708 806 519 469 StyI C'CwwG_G Cuts at: 513 1065 2610 2844 2877 3091 4137 4194 4291 Size: 552 1545 234 33 214 1046 57 97 Cuts at: 4291 5517 5634 6756 6963 7391 513 Size: 1226 117 1122 207 428 3074 Fragments arranged by size: 3074 1545 1226 1122 1046 552 428 234 214 207 117 97 57 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2130 3749 4218 4667 4865 6224 6722 Size: 1255 550 1619 469 449 198 1359 498 Cuts at: 6722 6830 6937 7979 7996 8149 8334 8779 325 Size: 108 107 1042 17 153 185 445 1498 Fragments arranged by size: 1619 1498 1359 1255 1042 550 498 469 449 445 198 185 153 108 107 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4845 4993 5022 Size: 274 80 33 51 1443 2868 148 29 Cuts at: 5022 5877 6403 8198 9910 96 Size: 855 526 1795 1712 138 Fragments arranged by size: 2868 1795 1712 1443 855 526 274 148 138 80 51 33 29 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3027 3059 3597 3629 4757 4789 9912 9944 3027 Size: 32 538 32 1128 32 5123 32 3035 Fragments arranged by size: 5123 3035 1128 538 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2458 5597 6083 1316 Size: 1142 3139 486 5185 Fragments arranged by size: 5185 3139 1142 486 XbaI T'CTAG_A Cuts at: 6838 7288 6838 Size: 450 9502 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2632 2875 4393 2632 Size: 243 1518 8191 Fragments arranged by size: 8191 1518 243 XhoI C'TCGA_G Cuts at: 1943 5162 1943 Size: 3219 6733 XmaI C'CCGG_G Cuts at: 1870 4017 5625 7277 1870 Size: 2147 1608 1652 4545 Fragments arranged by size: 4545 2147 1652 1608 XmnI GAAnn'nnTTC Cuts at: 3206 6006 6936 7145 8081 3206 Size: 2800 930 209 936 5077 Fragments arranged by size: 5077 2800 936 930 209 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7760 276 Size: 53 83 186 936 6226 2468 Fragments arranged by size: 6226 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI PacI PsrI RsrII SacII SrfI SwaI