Restriction map for
pFN21AA1179
(Linear) (Six-base) MAPSORT of: KIAA1179.seq Check: 2979 from: 1 to: 5247
KIAA1179.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2257 2434 5247
Size: 2257 177 2813
Fragments arranged by size:
2813 2257 177
AccI GT'mk_AC
Cuts at: 0 275 4718 5247
Size: 275 4443 529
Fragments arranged by size:
4443 529 275
Acc65I G'GTAC_C
Cuts at: 0 520 5247
Size: 520 4727
AclI AA'CG_TT
Cuts at: 0 1098 5247
Size: 1098 4149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 1488 2204 3143 3503 5247
Size: 413 1075 716 939 360 1744
Fragments arranged by size:
1744 1075 939 716 413 360
AflIII A'CryG_T
Cuts at: 0 2837 3188 3264 5247
Size: 2837 351 76 1983
Fragments arranged by size:
2837 1983 351 76
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2627 3384 5247
Size: 2627 757 1863
Fragments arranged by size:
2627 1863 757
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 709 741 2716 2748 5247
Size: 709 32 1975 32 2499
Fragments arranged by size:
2499 1975 709 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2238 4971 4983 5247
Size: 2238 2733 12 264
Fragments arranged by size:
2733 2238 264 12
ApaI G_GGCC'C
Cuts at: 0 3678 5247
Size: 3678 1569
ApoI r'AATT_y
Cuts at: 0 88 154 497 1300 3379 3469 3490 5095
Size: 88 66 343 803 2079 90 21 1605
Cuts at: 5095 5140 5194 5247
Size: 45 54 53
Fragments arranged by size:
2079 1605 803 343 90 88 66 54
53 45 21
AseI AT'TA_AT
Cuts at: 0 1334 2621 5247
Size: 1334 1287 2626
Fragments arranged by size:
2626 1334 1287
AvaI C'yCGr_G
Cuts at: 0 1034 2012 2363 3157 3424 3620 4988 5247
Size: 1034 978 351 794 267 196 1368 259
Fragments arranged by size:
1368 1034 978 794 351 267 259 196
AvrII C'CTAG_G
Cuts at: 0 1086 5247
Size: 1086 4161
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 512 545 722 755 5247
Size: 512 33 177 33 4492
Fragments arranged by size:
4492 512 177 33 33
BamHI G'GATC_C
Cuts at: 0 803 5247
Size: 803 4444
BanI G'GyrC_C
Cuts at: 0 520 1438 1659 2195 2674 4884 5002 5247
Size: 520 918 221 536 479 2210 118 245
Fragments arranged by size:
2210 918 536 520 479 245 221 118
BanII G_rGCy'C
Cuts at: 0 34 2333 2801 3678 4061 4413 4471 5049
Size: 34 2299 468 877 383 352 58 578
Cuts at: 5049 5222 5247
Size: 173 25
Fragments arranged by size:
2299 877 578 468 383 352 173 58
34 25
BbeI G_GCGC'C
Cuts at: 0 5006 5247
Size: 5006 241
BbsI GAAGACnn'nnnn_
Cuts at: 0 4172 5247
Size: 4172 1075
BbvCI CC'TCA_GC
Cuts at: 0 2792 3572 5247
Size: 2792 780 1675
Fragments arranged by size:
2792 1675 780
BciVI GTATCCnnnnn_n'
Cuts at: 0 2019 4857 5062 5247
Size: 2019 2838 205 185
Fragments arranged by size:
2838 2019 205 185
BfrBI ATG'CAT
Cuts at: 0 1226 2767 2891 5247
Size: 1226 1541 124 2356
Fragments arranged by size:
2356 1541 1226 124
BglI GCCn_nnn'nGGC
Cuts at: 0 3625 5011 5247
Size: 3625 1386 236
Fragments arranged by size:
3625 1386 236
BglII A'GATC_T
Cuts at: 0 2709 3075 5247
Size: 2709 366 2172
Fragments arranged by size:
2709 2172 366
BlpI GC'TnA_GC
Cuts at: 0 28 3560 4898 5247
Size: 28 3532 1338 349
Fragments arranged by size:
3532 1338 349 28
Bme1580I G_kGCm'C
Cuts at: 0 913 2198 2679 3678 3761 5247
Size: 913 1285 481 999 83 1486
Fragments arranged by size:
1486 1285 999 913 481 83
BmgBI CAC'GTC
Cuts at: 0 1608 2059 5187 5247
Size: 1608 451 3128 60
Fragments arranged by size:
3128 1608 451 60
BmrI ACTGGGnnnn_n'
Cuts at: 0 2542 3864 4950 5167 5247
Size: 2542 1322 1086 217 80
Fragments arranged by size:
2542 1322 1086 217 80
BmtI G_CTAG'C
Cuts at: 0 2302 2380 5247
Size: 2302 78 2867
Fragments arranged by size:
2867 2302 78
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1060 3749 4389 4543 4587 5247
Size: 1060 2689 640 154 44 660
Fragments arranged by size:
2689 1060 660 640 154 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 1729 2227 2506 3136 3475 3790 3887
Size: 578 1151 498 279 630 339 315 97
Cuts at: 3887 4559 4993 5247
Size: 672 434 254
Fragments arranged by size:
1151 672 630 578 498 434 339 315
279 254 97
Bpu10I CC'TnA_GC
Cuts at: 0 2792 3572 4142 5247
Size: 2792 780 570 1105
Fragments arranged by size:
2792 1105 780 570
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1030 1911 3224 3394 4183 5247
Size: 1030 881 1313 170 789 1064
Fragments arranged by size:
1313 1064 1030 881 789 170
BsaI GGTCTCn'nnnn_
Cuts at: 0 1978 2889 3675 5247
Size: 1978 911 786 1572
Fragments arranged by size:
1978 1572 911 786
BsaAI yAC'GTr
Cuts at: 0 462 3267 5247
Size: 462 2805 1980
Fragments arranged by size:
2805 1980 462
BsaHI Gr'CG_yC
Cuts at: 0 5003 5247
Size: 5003 244
BsaWI w'CCGG_w
Cuts at: 0 1511 2720 4911 5247
Size: 1511 1209 2191 336
Fragments arranged by size:
2191 1511 1209 336
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 709 739 1481 1511 1561 1591 3130 3160
Size: 709 30 742 30 50 30 1539 30
Cuts at: 3160 3355 3385 4999 5029 5247
Size: 195 30 1614 30 218
Fragments arranged by size:
1614 1539 742 709 218 195 50 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1489 2140 2272 2584 2809 3070 3311 3649
Size: 1489 651 132 312 225 261 241 338
Cuts at: 3649 4129 4600 5247
Size: 480 471 647
Fragments arranged by size:
1489 651 647 480 471 338 312 261
241 225 132
BseYI C'CCAG_C
Cuts at: 0 1134 2199 2326 3327 3760 4019 4505 4703
Size: 1134 1065 127 1001 433 259 486 198
Cuts at: 4703 4763 4893 5224 5247
Size: 60 130 331 23
Fragments arranged by size:
1134 1065 1001 486 433 331 259 198
130 127 60 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 2614 4067 4309 5247
Size: 84 2530 1453 242 938
Fragments arranged by size:
2530 1453 938 242 84
BsiHKAI G_wGCw'C
Cuts at: 0 1046 2058 2647 2801 4061 4471 5035 5247
Size: 1046 1012 589 154 1260 410 564 212
Fragments arranged by size:
1260 1046 1012 589 564 410 212 154
BsmI GAATG_Cn'
Cuts at: 0 2121 2509 4777 5247
Size: 2121 388 2268 470
Fragments arranged by size:
2268 2121 470 388
Bsp1286I G_dGCh'C
Cuts at: 0 34 913 1046 2058 2198 2333 2647 2679
Size: 34 879 133 1012 140 135 314 32
Cuts at: 2679 2801 3678 3761 4061 4413 4471 5035 5049
Size: 122 877 83 300 352 58 564 14
Cuts at: 5049 5222 5247
Size: 173 25
Fragments arranged by size:
1012 879 877 564 352 314 300 173
140 135 133 122 83 58 34 32
25 14
BspHI T'CATG_A
Cuts at: 0 1304 2513 5247
Size: 1304 1209 2734
Fragments arranged by size:
2734 1304 1209
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 67 2257 2434 4968 5247
Size: 67 2190 177 2534 279
Fragments arranged by size:
2534 2190 279 177 67
BsrBI CCG'CTC
Cuts at: 0 720 1713 3795 5247
Size: 720 993 2082 1452
Fragments arranged by size:
2082 1452 993 720
BsrDI GCAATG_nn'
Cuts at: 0 361 1197 1883 1925 4742 5247
Size: 361 836 686 42 2817 505
Fragments arranged by size:
2817 836 686 505 361 42
BsrFI r'CCGG_y
Cuts at: 0 360 5247
Size: 360 4887
BsrGI T'GTAC_A
Cuts at: 0 2840 3017 4398 5247
Size: 2840 177 1381 849
Fragments arranged by size:
2840 1381 849 177
BssSI C'ACGA_G
Cuts at: 0 2749 5247
Size: 2749 2498
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4096 4744 4983 5247
Size: 4096 648 239 264
Fragments arranged by size:
4096 648 264 239
BstBI TT'CG_AA
Cuts at: 0 3473 5247
Size: 3473 1774
BstEII G'GTnAC_C
Cuts at: 0 54 5247
Size: 54 5193
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2293 3768 3825 4027 5247
Size: 2293 1475 57 202 1220
Fragments arranged by size:
2293 1475 1220 202 57
BstYI r'GATC_y
Cuts at: 0 803 1180 1413 2709 3075 4152 4448 5247
Size: 803 377 233 1296 366 1077 296 799
Fragments arranged by size:
1296 1077 803 799 377 366 296 233
Bsu36I CC'TnA_GG
Cuts at: 0 16 35 713 5247
Size: 16 19 678 4534
Fragments arranged by size:
4534 678 19 16
BtgI C'CryG_G
Cuts at: 0 2132 3884 4664 4958 5247
Size: 2132 1752 780 294 289
Fragments arranged by size:
2132 1752 780 294 289
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2896 5247
Size: 2896 2351
BtsI GCAGTG_nn'
Cuts at: 0 866 1126 1594 2405 2726 5247
Size: 866 260 468 811 321 2521
Fragments arranged by size:
2521 866 811 468 321 260
ClaI AT'CG_AT
Cuts at: 0 4094 5247
Size: 4094 1153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 222 257 5247
Size: 222 35 4990
Fragments arranged by size:
4990 222 35
DraIII CAC_nnn'GTG
Cuts at: 0 919 2592 5247
Size: 919 1673 2655
Fragments arranged by size:
2655 1673 919
DrdI GACnn_nn'nnGTC
Cuts at: 0 115 307 5247
Size: 115 192 4940
Fragments arranged by size:
4940 192 115
EaeI y'GGCC_r
Cuts at: 0 358 3230 5104 5150 5247
Size: 358 2872 1874 46 97
Fragments arranged by size:
2872 1874 358 97 46
EarI CTCTTCn'nnn_
Cuts at: 0 187 821 1067 1507 3209 3470 4534 4922
Size: 187 634 246 440 1702 261 1064 388
Cuts at: 4922 5247
Size: 325
Fragments arranged by size:
1702 1064 634 440 388 325 261 246
187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2039 3040 3946 5247
Size: 2039 1001 906 1301
Fragments arranged by size:
2039 1301 1001 906
EcoICRI GAG'CTC
Cuts at: 0 2799 4059 4469 5247
Size: 2799 1260 410 778
Fragments arranged by size:
2799 1260 778 410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 578 1488 1729 2204 2227 2506 3136
Size: 413 165 910 241 475 23 279 630
Cuts at: 3136 3143 3475 3503 3790 3887 4559 4993 5247
Size: 7 332 28 287 97 672 434 254
Fragments arranged by size:
910 672 630 475 434 413 332 287
279 254 241 165 97 28 23 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1090 2419 4081 5247
Size: 1090 1329 1662 1166
Fragments arranged by size:
1662 1329 1166 1090
EcoO109I rG'GnC_Cy
Cuts at: 0 20 994 1140 1593 1782 2481 3626 4015
Size: 20 974 146 453 189 699 1145 389
Cuts at: 4015 5247
Size: 1232
Fragments arranged by size:
1232 1145 974 699 453 389 189 146
20
EcoRI G'AATT_C
Cuts at: 0 497 1300 3490 5247
Size: 497 803 2190 1757
Fragments arranged by size:
2190 1757 803 497
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1221 1518 5247
Size: 1221 297 3729
Fragments arranged by size:
3729 1221 297
FspI TGC'GCA
Cuts at: 0 3739 5247
Size: 3739 1508
HaeII r_GCGC'y
Cuts at: 0 5006 5247
Size: 5006 241
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 142 174 538 570 1481 1513 1986 1993
Size: 142 32 364 32 911 32 473 7
Cuts at: 1993 2018 2025 2454 2486 3355 3387 5247
Size: 25 7 429 32 869 32 1860
Fragments arranged by size:
1860 911 869 473 429 364 142 32
32 32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 0 582 983 3351 5247
Size: 582 401 2368 1896
Fragments arranged by size:
2368 1896 582 401
HpaI GTT'AAC
Cuts at: 0 582 5247
Size: 582 4665
KasI G'GCGC_C
Cuts at: 0 5002 5247
Size: 5002 245
KpnI G_GTAC'C
Cuts at: 0 524 5247
Size: 524 4723
MfeI C'AATT_G
Cuts at: 0 2657 3366 5247
Size: 2657 709 1881
Fragments arranged by size:
2657 1881 709
MscI TGG'CCA
Cuts at: 0 3232 5152 5247
Size: 3232 1920 95
Fragments arranged by size:
3232 1920 95
MslI CAynn'nnrTG
Cuts at: 0 515 1532 2137 2836 3013 4331 4459 4619
Size: 515 1017 605 699 177 1318 128 160
Cuts at: 4619 5247
Size: 628
Fragments arranged by size:
1318 1017 699 628 605 515 177 160
128
MspA1I CmG'CkG
Cuts at: 0 172 736 3768 3923 3955 4023 4051 4656
Size: 172 564 3032 155 32 68 28 605
Cuts at: 4656 4968 5247
Size: 312 279
Fragments arranged by size:
3032 605 564 312 279 172 155 68
32 28
NaeI GCC'GGC
Cuts at: 0 362 5247
Size: 362 4885
NarI GG'CG_CC
Cuts at: 0 5003 5247
Size: 5003 244
NcoI C'CATG_G
Cuts at: 0 2132 4958 5247
Size: 2132 2826 289
Fragments arranged by size:
2826 2132 289
NgoMIV G'CCGG_C
Cuts at: 0 360 5247
Size: 360 4887
NheI G'CTAG_C
Cuts at: 0 2298 2376 5247
Size: 2298 78 2871
Fragments arranged by size:
2871 2298 78
NsiI A_TGCA'T
Cuts at: 0 1228 2769 2893 5247
Size: 1228 1541 124 2354
Fragments arranged by size:
2354 1541 1228 124
NspI r_CATG'y
Cuts at: 0 684 2142 2841 3192 4909 5247
Size: 684 1458 699 351 1717 338
Fragments arranged by size:
1717 1458 699 684 351 338
PasI CC'CwG_GG
Cuts at: 0 1281 2670 5247
Size: 1281 1389 2577
Fragments arranged by size:
2577 1389 1281
PciI A'CATG_T
Cuts at: 0 2837 3188 5247
Size: 2837 351 2059
Fragments arranged by size:
2837 2059 351
PflMI CCAn_nnn'nTGG
Cuts at: 0 2877 2940 4386 4971 5247
Size: 2877 63 1446 585 276
Fragments arranged by size:
2877 1446 585 276 63
PfoI T'CCnGG_A
Cuts at: 0 3335 3452 4361 4473 5247
Size: 3335 117 909 112 774
Fragments arranged by size:
3335 909 774 117 112
PmlI CAC'GTG
Cuts at: 0 3267 5247
Size: 3267 1980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 709 741 4916 4948 5247
Size: 709 32 4175 32 299
Fragments arranged by size:
4175 709 299 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 20 994 1140 1593 4015 5247
Size: 20 974 146 453 2422 1232
Fragments arranged by size:
2422 1232 974 453 146 20
PsiI TTA'TAA
Cuts at: 0 3707 4187 5247
Size: 3707 480 1060
Fragments arranged by size:
3707 1060 480
PspOMI G'GGCC_C
Cuts at: 0 3674 5247
Size: 3674 1573
PspXI vC'TCGA_Gb
Cuts at: 0 3157 5247
Size: 3157 2090
PstI C_TGCA'G
Cuts at: 0 639 2412 2777 3309 3363 3782 4056 4638
Size: 639 1773 365 532 54 419 274 582
Cuts at: 4638 5247
Size: 609
Fragments arranged by size:
1773 639 609 582 532 419 365 274
54
PvuII CAG'CTG
Cuts at: 0 172 736 3768 3955 4023 4051 4656 5247
Size: 172 564 3032 187 68 28 605 591
Fragments arranged by size:
3032 605 591 564 187 172 68 28
SacI G_AGCT'C
Cuts at: 0 2801 4061 4471 5247
Size: 2801 1260 410 776
Fragments arranged by size:
2801 1260 776 410
SanDI GG'GwC_CC
Cuts at: 0 1593 5247
Size: 1593 3654
SapI GCTCTTCn'nnn_
Cuts at: 0 187 5247
Size: 187 5060
SbfI CC_TGCA'GG
Cuts at: 0 2777 5247
Size: 2777 2470
SexAI A'CCwGG_T
Cuts at: 0 2588 4547 5247
Size: 2588 1959 700
Fragments arranged by size:
2588 1959 700
SfcI C'TryA_G
Cuts at: 0 635 1406 2275 2408 2713 2773 3133 3222
Size: 635 771 869 133 305 60 360 89
Cuts at: 3222 3305 3359 3778 4010 4052 4634 4715 5247
Size: 83 54 419 232 42 582 81 532
Fragments arranged by size:
869 771 635 582 532 419 360 305
232 133 89 83 81 60 54 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3625 5247
Size: 3625 1622
SfoI GGC'GCC
Cuts at: 0 5004 5247
Size: 5004 243
SmaI CCC'GGG
Cuts at: 0 2014 3622 5247
Size: 2014 1608 1625
Fragments arranged by size:
2014 1625 1608
SmlI C'TyrA_G
Cuts at: 0 1045 1890 3157 3239 3409 4198 5247
Size: 1045 845 1267 82 170 789 1049
Fragments arranged by size:
1267 1049 1045 845 789 170 82
StuI AGG'CCT
Cuts at: 0 3220 3689 4495 5014 5247
Size: 3220 469 806 519 233
Fragments arranged by size:
3220 806 519 469 233
StyI C'CwwG_G
Cuts at: 0 605 839 872 1086 2132 2189 2286 3512
Size: 605 234 33 214 1046 57 97 1226
Cuts at: 3512 3629 4751 4958 5247
Size: 117 1122 207 289
Fragments arranged by size:
1226 1122 1046 605 289 234 214 207
117 97 57 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 125 1744 2213 2662 2860 4219 4717 4825
Size: 125 1619 469 449 198 1359 498 108
Cuts at: 4825 4932 5247
Size: 107 315
Fragments arranged by size:
1619 1359 498 469 449 315 198 125
108 107
TatI w'GTAC_w
Cuts at: 0 2840 2988 3017 3872 4398 5247
Size: 2840 148 29 855 526 849
Fragments arranged by size:
2840 855 849 526 148 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1022 1054 1592 1624 2752 2784 5247
Size: 1022 32 538 32 1128 32 2463
Fragments arranged by size:
2463 1128 1022 538 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 453 3592 4078 5247
Size: 453 3139 486 1169
Fragments arranged by size:
3139 1169 486 453
XbaI T'CTAG_A
Cuts at: 0 4833 5247
Size: 4833 414
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 627 870 2388 5247
Size: 627 243 1518 2859
Fragments arranged by size:
2859 1518 627 243
XhoI C'TCGA_G
Cuts at: 0 3157 5247
Size: 3157 2090
XmaI C'CCGG_G
Cuts at: 0 2012 3620 5247
Size: 2012 1608 1627
Fragments arranged by size:
2012 1627 1608
XmnI GAAnn'nnTTC
Cuts at: 0 1201 4001 4931 5140 5247
Size: 1201 2800 930 209 107
Fragments arranged by size:
2800 1201 930 209 107
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AloI
AlwNI ApaI ApoI AseI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI
PmlI PpiI PpuMI PsiI PspOMI PspXI PstI PvuII
SacI SanDI SapI SbfI SexAI SfcI SfiI SfoI
SmaI SmlI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI ApaLI AscI
AsiSI BcgI BclI BsaBI BsiEI BsiWI BsmBI BspEI
BssHII BstZ17I DraI EagI EcoRV FseI FspAI HindIII
MluI MmeI NdeI NotI NruI PacI PmeI PshAI
PsrI PvuI RsrII SacII SalI ScaI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI ZraI