(Circular) (Six-base) MAPSORT of: pFN21AA1168.seq Check: 291 from: 1 to: 8539 pFN21AA1168 8539 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2902 3376 5631 2902 Size: 474 2255 5810 Fragments arranged by size: 5810 2255 474 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6349 278 Size: 53 83 186 936 4813 2468 Fragments arranged by size: 4813 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4185 5181 5882 1937 Size: 2248 996 701 4594 Fragments arranged by size: 4594 2248 996 701 Acc65I G'GTAC_C Cuts at: 5860 5860 Size: 8539 AclI AA'CG_TT Cuts at: 1563 4252 6666 7039 1563 Size: 2689 2414 373 3063 Fragments arranged by size: 3063 2689 2414 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4346 5036 5699 6602 7986 8254 1632 Size: 2714 690 663 903 1384 268 1917 Fragments arranged by size: 2714 1917 1384 903 690 663 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8342 AflIII A'CryG_T Cuts at: 1203 2502 4336 5052 7438 8415 1203 Size: 1299 1834 716 2386 977 1327 Fragments arranged by size: 2386 1834 1327 1299 977 716 AgeI A'CCGG_T Cuts at: 7370 7370 Size: 8539 AhdI GACnn_n'nnGTC Cuts at: 3839 7268 3839 Size: 3429 5110 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8539 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8539 AlwNI CAG_nnn'CTG Cuts at: 2587 4135 5065 6282 7854 2587 Size: 1548 930 1217 1572 3272 Fragments arranged by size: 3272 1572 1548 1217 930 ApaI G_GGCC'C Cuts at: 5411 5411 Size: 8539 ApaLI G'TGCA_C Cuts at: 3004 6596 7752 3004 Size: 3592 1156 3791 Fragments arranged by size: 3791 3592 1156 ApoI r'AATT_y Cuts at: 1493 2894 3344 3950 4795 5848 6074 6226 8335 Size: 1401 450 606 845 1053 226 152 2109 Cuts at: 8335 1493 Size: 1697 Fragments arranged by size: 2109 1697 1401 1053 845 606 450 226 152 AseI AT'TA_AT Cuts at: 160 1032 2874 7093 160 Size: 872 1842 4219 1606 Fragments arranged by size: 4219 1842 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8539 AvaI C'yCGr_G Cuts at: 1870 1943 2274 3691 5864 1870 Size: 73 331 1417 2173 4545 Fragments arranged by size: 4545 2173 1417 331 73 BamHI G'GATC_C Cuts at: 4745 4989 5869 6236 4745 Size: 244 880 367 7048 Fragments arranged by size: 7048 880 367 244 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2917 3290 3640 5860 7315 Size: 325 217 627 1130 373 350 2220 1455 Cuts at: 7315 618 Size: 1842 Fragments arranged by size: 2220 1842 1455 1130 627 373 350 325 217 BanII G_rGCy'C Cuts at: 1391 3093 3128 3171 3486 3582 5043 5411 5858 Size: 1702 35 43 315 96 1461 368 447 Cuts at: 5858 1391 Size: 4072 Fragments arranged by size: 4072 1702 1461 447 368 315 96 43 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2339 3406 3815 4597 928 Size: 971 440 1067 409 782 4870 Fragments arranged by size: 4870 1067 971 782 440 409 BbvCI CC'TCA_GC Cuts at: 4155 5283 4155 Size: 1128 7411 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2383 2417 3720 3754 6730 6764 1903 Size: 34 446 34 1303 34 2976 34 3678 Fragments arranged by size: 3678 2976 1303 446 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4305 6431 7641 4305 Size: 2126 1210 5203 Fragments arranged by size: 5203 2126 1210 BclI T'GATC_A Cuts at: 1548 3372 4174 1548 Size: 1824 802 5913 Fragments arranged by size: 5913 1824 802 BfrBI ATG'CAT Cuts at: 2088 2156 2088 Size: 68 8471 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3490 3735 7150 Size: 107 122 71 631 605 1818 245 3415 Cuts at: 7150 136 Size: 1525 Fragments arranged by size: 3415 1818 1525 631 605 245 122 107 71 BglII A'GATC_T Cuts at: 2322 4203 5712 8534 2322 Size: 1881 1509 2822 2327 Fragments arranged by size: 2822 2327 1881 1509 BlpI GC'TnA_GC Cuts at: 5523 5956 5523 Size: 433 8106 Bme1580I G_kGCm'C Cuts at: 1790 2922 3008 3645 5411 5676 6600 7756 1790 Size: 1132 86 637 1766 265 924 1156 2573 Fragments arranged by size: 2573 1766 1156 1132 924 637 265 86 BmgBI CAC'GTC Cuts at: 1318 2017 3003 3223 4339 5053 5437 1318 Size: 699 986 220 1116 714 384 4420 Fragments arranged by size: 4420 1116 986 714 699 384 220 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2602 2662 2769 Size: 416 53 83 399 24 1185 60 107 Cuts at: 2769 3022 3571 5560 6270 7228 442 Size: 253 549 1989 710 958 1753 Fragments arranged by size: 1989 1753 1185 958 710 549 416 399 253 107 83 60 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8539 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3253 4996 3253 Size: 1743 6796 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2188 2243 2369 3980 4490 4502 4898 5015 Size: 949 55 126 1611 510 12 396 117 Cuts at: 5015 5225 7199 1239 Size: 210 1974 2579 Fragments arranged by size: 2579 1974 1611 949 510 396 210 126 117 55 12 Bpu10I CC'TnA_GC Cuts at: 3127 4155 5283 3127 Size: 1028 1128 6383 Fragments arranged by size: 6383 1128 1028 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2780 3619 6664 7529 7827 2780 Size: 839 3045 865 298 3492 Fragments arranged by size: 3492 3045 865 839 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2957 7202 882 Size: 617 1458 4245 2219 Fragments arranged by size: 4245 2219 1458 617 BsaAI yAC'GTr Cuts at: 493 1204 6330 493 Size: 711 5126 2702 Fragments arranged by size: 5126 2702 711 BsaBI GATnn'nnATC Cuts at: 8533 8533 Size: 8539 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4281 5427 6346 6728 Size: 53 83 186 936 2748 1146 919 382 Cuts at: 6728 7363 275 Size: 635 1451 Fragments arranged by size: 2748 1451 1146 936 919 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5211 5694 6972 7370 7644 7791 8202 5211 Size: 483 1278 398 274 147 411 5548 Fragments arranged by size: 5548 1278 483 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2775 2805 4439 4457 4469 Size: 30 165 30 1372 30 1634 18 12 Cuts at: 4469 4487 5318 5348 1178 Size: 18 831 30 4369 Fragments arranged by size: 4369 1634 1372 831 165 30 30 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2723 2783 3170 3832 5090 5141 1186 Size: 1537 60 387 662 1258 51 4584 Fragments arranged by size: 4584 1537 1258 662 387 60 51 BseYI C'CCAG_C Cuts at: 3933 4383 5272 5578 7742 8238 3933 Size: 450 889 306 2164 496 4234 Fragments arranged by size: 4234 2164 889 496 450 306 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2560 5654 1163 Size: 573 824 3094 4048 Fragments arranged by size: 4048 3094 824 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5695 5989 6750 7778 1540 Size: 275 186 3694 294 761 1028 2301 Fragments arranged by size: 3694 2301 1028 761 294 275 186 BsiHKAI G_wGCw'C Cuts at: 2974 3008 3093 3171 3393 5043 6600 6685 7756 Size: 34 85 78 222 1650 1557 85 1071 Cuts at: 7756 2974 Size: 3757 Fragments arranged by size: 3757 1650 1557 1071 222 85 85 78 34 BsmI GAATG_Cn' Cuts at: 2158 5373 6050 6143 2158 Size: 3215 677 93 4554 Fragments arranged by size: 4554 3215 677 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2922 2974 3008 3093 3128 3171 3393 Size: 399 1132 52 34 85 35 43 222 Cuts at: 3393 3486 3582 3645 5043 5411 5676 5858 6600 Size: 93 96 63 1398 368 265 182 742 Cuts at: 6600 6685 7756 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1398 1132 1071 742 399 368 265 222 182 96 93 85 85 63 52 43 35 34 BspEI T'CCGG_A Cuts at: 5211 8202 5211 Size: 2991 5548 BspHI T'CATG_A Cuts at: 2370 6428 8158 2370 Size: 4058 1730 2751 Fragments arranged by size: 4058 2751 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2058 2902 3376 4723 5394 5631 5894 844 Size: 1214 844 474 1347 671 237 263 3489 Fragments arranged by size: 3489 1347 1214 844 671 474 263 237 BsrBI CCG'CTC Cuts at: 1394 1823 2407 5245 5269 5329 6426 1394 Size: 429 584 2838 24 60 1097 3507 Fragments arranged by size: 3507 2838 1097 584 429 60 24 BsrDI GCAATG_nn' Cuts at: 65 1248 2169 3449 3783 4761 5086 5295 7034 Size: 1183 921 1280 334 978 325 209 1739 Cuts at: 7034 7208 65 Size: 174 1396 Fragments arranged by size: 1739 1396 1280 1183 978 921 334 325 209 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4491 5460 7183 7370 1700 Size: 110 2681 969 1723 187 2869 Fragments arranged by size: 2869 2681 1723 969 187 110 BsrGI T'GTAC_A Cuts at: 96 2253 3753 4086 96 Size: 2157 1500 333 4549 Fragments arranged by size: 4549 2157 1500 333 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8539 BssSI C'ACGA_G Cuts at: 6599 7611 6599 Size: 1012 7527 BstAPI GCAn_nnn'nTGC Cuts at: 794 2917 4507 794 Size: 2123 1590 4826 Fragments arranged by size: 4826 2123 1590 BstBI TT'CG_AA Cuts at: 3544 7343 3544 Size: 3799 4740 BstEII G'GTnAC_C Cuts at: 1184 2359 5309 1184 Size: 1175 2950 4414 Fragments arranged by size: 4414 2950 1175 BstXI CCAn_nnnn'nTGG Cuts at: 2644 2644 Size: 8539 BstYI r'GATC_y Cuts at: 1972 2322 2602 3057 4081 4203 4745 4989 5712 Size: 350 280 455 1024 122 542 244 723 Cuts at: 5712 5869 6236 6621 6638 8090 8176 8188 8534 Size: 157 367 385 17 1452 86 12 346 Cuts at: 8534 1972 Size: 1977 Fragments arranged by size: 1977 1452 1024 723 542 455 385 367 350 346 280 244 157 122 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3815 5066 3815 Size: 1251 7288 BtgI C'CryG_G Cuts at: 513 1065 3294 3969 4224 5760 513 Size: 552 2229 675 255 1536 3292 Fragments arranged by size: 3292 2229 1536 675 552 255 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4837 5553 487 Size: 683 56 3611 716 3473 Fragments arranged by size: 3611 3473 716 683 56 BtsI GCAGTG_nn' Cuts at: 3923 4240 4319 5534 6055 6849 6869 3923 Size: 317 79 1215 521 794 20 5593 Fragments arranged by size: 5593 1215 794 521 317 79 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8539 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8504 DraI TTT'AAA Cuts at: 4123 5843 4123 Size: 1720 6819 DraIII CAC_nnn'GTG Cuts at: 2670 4570 2670 Size: 1900 6639 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7546 809 Size: 507 6230 1802 Fragments arranged by size: 6230 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2646 2949 4585 Size: 54 1425 50 275 18 816 303 1636 Cuts at: 4585 5688 5826 5986 6875 8504 8 Size: 1103 138 160 889 1629 43 Fragments arranged by size: 1636 1629 1425 1103 889 816 303 275 160 138 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5986 1537 Size: 275 4174 4090 Fragments arranged by size: 4174 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2861 2976 3176 3727 4883 5096 6469 Size: 699 809 115 200 551 1156 213 1373 Cuts at: 6469 1353 Size: 3423 Fragments arranged by size: 3423 1373 1156 809 699 551 213 200 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 7126 7500 7646 387 Size: 1064 138 2975 2562 374 146 1280 Fragments arranged by size: 2975 2562 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3091 3169 5041 3091 Size: 78 1872 6589 Fragments arranged by size: 6589 1872 78 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2188 2243 2369 3980 4346 4490 4502 Size: 393 556 55 126 1611 366 144 12 Cuts at: 4502 4898 5015 5036 5225 5699 6602 7199 7986 Size: 396 117 21 189 474 903 597 787 Cuts at: 7986 8254 1239 Size: 268 1524 Fragments arranged by size: 1611 1524 903 787 597 556 474 396 393 366 268 189 144 126 117 55 21 12 EcoNI CCTnn'n_nnAGG Cuts at: 2569 3491 5321 2569 Size: 922 1830 5787 Fragments arranged by size: 5787 1830 922 EcoO109I rG'GnC_Cy Cuts at: 3613 4447 5277 5392 5407 3613 Size: 834 830 115 15 6745 Fragments arranged by size: 6745 834 830 115 15 EcoRI G'AATT_C Cuts at: 3950 5848 6226 3950 Size: 1898 378 6263 Fragments arranged by size: 6263 1898 378 EcoRV GAT'ATC Cuts at: 3634 3634 Size: 8539 FspI TGC'GCA Cuts at: 7045 7045 Size: 8539 HaeII r_GCGC'y Cuts at: 1678 3219 4101 5203 5475 7686 1678 Size: 1541 882 1102 272 2211 2531 Fragments arranged by size: 2531 2211 1541 1102 882 272 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 2307 2339 2447 Size: 32 135 32 449 32 254 32 108 Cuts at: 2447 2479 2587 2594 2619 2626 2860 2892 2956 Size: 32 108 7 25 7 234 32 64 Cuts at: 2956 2975 2988 3007 3618 3650 3825 3857 4439 Size: 19 13 19 611 32 175 32 582 Cuts at: 4439 4471 5169 5201 5206 5238 7180 7212 7254 Size: 32 698 32 5 32 1942 32 42 Cuts at: 7254 7286 1373 Size: 32 2626 Fragments arranged by size: 2626 1942 698 611 582 449 254 234 175 135 108 108 64 42 32 32 32 32 32 32 32 32 32 32 32 32 32 25 19 19 13 7 7 5 HincII GTy'rAC Cuts at: 669 1657 1938 2731 3966 5883 6129 669 Size: 988 281 793 1235 1917 246 3079 Fragments arranged by size: 3079 1917 1235 988 793 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8539 HpaI GTT'AAC Cuts at: 3966 6129 3966 Size: 2163 6376 KpnI G_GTAC'C Cuts at: 5864 5864 Size: 8539 MfeI C'AATT_G Cuts at: 4359 6138 4359 Size: 1779 6760 MluI A'CGCG_T Cuts at: 8415 8415 Size: 8539 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2064 4224 7653 7837 1305 Size: 759 2160 3429 184 2007 Fragments arranged by size: 3429 2160 2007 759 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2648 2951 5828 10 Size: 54 1425 343 816 303 2877 2721 Fragments arranged by size: 2877 2721 1425 816 343 303 54 MslI CAynn'nnrTG Cuts at: 518 1166 2238 2501 2738 3230 3998 4856 4974 Size: 648 1072 263 237 492 768 858 118 Cuts at: 4974 5439 5498 6497 6856 7015 518 Size: 465 59 999 359 159 2042 Fragments arranged by size: 2042 1072 999 858 768 648 492 465 359 263 237 159 118 59 MspA1I CmG'CkG Cuts at: 1606 2268 2908 3296 4345 5014 5522 5955 6632 Size: 662 640 388 1049 669 508 433 677 Cuts at: 6632 7780 8025 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1049 677 669 662 640 508 433 388 245 NcoI C'CATG_G Cuts at: 513 1065 3969 4224 513 Size: 552 2904 255 4828 Fragments arranged by size: 4828 2904 552 255 NdeI CA'TA_TG Cuts at: 387 2787 4521 387 Size: 2400 1734 4405 Fragments arranged by size: 4405 2400 1734 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8539 NotI GC'GGCC_GC Cuts at: 5986 5986 Size: 8539 NruI TCG'CGA Cuts at: 3165 5304 3165 Size: 2139 6400 NsiI A_TGCA'T Cuts at: 2090 2158 2090 Size: 68 8471 NspI r_CATG'y Cuts at: 1132 1229 2506 2938 4604 5792 5897 7442 1132 Size: 97 1277 432 1666 1188 105 1545 2229 Fragments arranged by size: 2229 1666 1545 1277 1188 432 105 97 PasI CC'CwG_GG Cuts at: 1348 4985 1348 Size: 3637 4902 PciI A'CATG_T Cuts at: 2502 7438 2502 Size: 4936 3603 PflMI CCAn_nnn'nTGG Cuts at: 1108 3226 4011 4033 4348 5824 1108 Size: 2118 785 22 315 1476 3823 Fragments arranged by size: 3823 2118 1476 785 315 22 PfoI T'CCnGG_A Cuts at: 2514 2616 3060 3957 4032 4992 2514 Size: 102 444 897 75 960 6061 Fragments arranged by size: 6061 960 897 444 102 75 PmeI GTTT'AAAC Cuts at: 5843 5843 Size: 8539 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6608 6640 8121 8153 6608 Size: 32 1481 32 6994 Fragments arranged by size: 6994 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3613 4447 5277 5392 3613 Size: 834 830 115 6760 Fragments arranged by size: 6760 834 830 115 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8539 PsiI TTA'TAA Cuts at: 6109 6109 Size: 8539 PspOMI G'GGCC_C Cuts at: 5407 5407 Size: 8539 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2747 2779 3425 3457 2747 Size: 32 646 32 7829 Fragments arranged by size: 7829 646 32 32 PstI C_TGCA'G Cuts at: 830 2417 2913 3198 3387 3915 4832 5391 5891 Size: 1587 496 285 189 528 917 559 500 Cuts at: 5891 830 Size: 3478 Fragments arranged by size: 3478 1587 917 559 528 500 496 285 189 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8539 PvuII CAG'CTG Cuts at: 2268 2908 4345 5014 5522 2268 Size: 640 1437 669 508 5285 Fragments arranged by size: 5285 1437 669 640 508 SacI G_AGCT'C Cuts at: 3093 3171 5043 3093 Size: 78 1872 6589 Fragments arranged by size: 6589 1872 78 SacII CC_GC'GG Cuts at: 3297 3297 Size: 8539 SalI G'TCGA_C Cuts at: 1936 5881 1936 Size: 3945 4594 SanDI GG'GwC_CC Cuts at: 5277 5277 Size: 8539 SapI GCTCTTCn'nnn_ Cuts at: 2052 3176 4883 2052 Size: 1124 1707 5708 Fragments arranged by size: 5708 1707 1124 SbfI CC_TGCA'GG Cuts at: 3387 5391 5891 3387 Size: 2004 500 6035 Fragments arranged by size: 6035 2004 500 ScaI AGT'ACT Cuts at: 6787 8499 6787 Size: 1712 6827 SexAI A'CCwGG_T Cuts at: 5250 5250 Size: 8539 SfcI C'TryA_G Cuts at: 826 1045 2193 2413 2909 3100 3194 3383 3533 Size: 219 1148 220 496 191 94 189 150 Cuts at: 3533 3911 4768 4780 4789 4828 5387 5887 7022 Size: 378 857 12 9 39 559 500 1135 Cuts at: 7022 7703 7894 826 Size: 681 191 1471 Fragments arranged by size: 1471 1148 1135 857 681 559 500 496 378 220 219 191 191 189 150 94 39 12 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8539 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8539 SmaI CCC'GGG Cuts at: 1872 2276 5866 1872 Size: 404 3590 4545 Fragments arranged by size: 4545 3590 404 SmlI C'TyrA_G Cuts at: 820 1017 1943 2795 3598 6643 7544 7806 820 Size: 197 926 852 803 3045 901 262 1553 Fragments arranged by size: 3045 1553 926 901 852 803 262 197 SnaBI TAC'GTA Cuts at: 493 6330 493 Size: 5837 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8539 SphI G_CATG'C Cuts at: 1132 5897 1132 Size: 4765 3774 SspI AAT'ATT Cuts at: 5 52 6463 5 Size: 47 6411 2081 Fragments arranged by size: 6411 2081 47 StuI AGG'CCT Cuts at: 1517 2471 3381 3677 1517 Size: 954 910 296 6379 Fragments arranged by size: 6379 954 910 296 StyI C'CwwG_G Cuts at: 513 1065 2485 3969 4224 4413 4900 5322 5823 Size: 552 1420 1484 255 189 487 422 501 Cuts at: 5823 5978 513 Size: 155 3074 Fragments arranged by size: 3074 1484 1420 552 501 487 422 255 189 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2681 3023 4706 5824 6566 6583 6736 Size: 1255 1101 342 1683 1118 742 17 153 Cuts at: 6736 6921 7366 325 Size: 185 445 1498 Fragments arranged by size: 1683 1498 1255 1118 1101 742 445 342 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2253 3030 3234 Size: 274 80 33 51 1443 276 777 204 Cuts at: 3234 3753 4086 5487 6785 8497 96 Size: 519 333 1401 1298 1712 138 Fragments arranged by size: 1712 1443 1401 1298 777 519 333 276 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3854 3886 4457 4489 4556 4588 5318 5350 8499 Size: 32 571 32 67 32 730 32 3149 Cuts at: 8499 8531 3854 Size: 32 3862 Fragments arranged by size: 3862 3149 730 571 67 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2459 2726 1316 Size: 1143 267 7129 Fragments arranged by size: 7129 1143 267 XbaI T'CTAG_A Cuts at: 5875 5875 Size: 8539 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4465 4465 Size: 8539 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8539 XmaI C'CCGG_G Cuts at: 1870 2274 5864 1870 Size: 404 3590 4545 Fragments arranged by size: 4545 3590 404 XmnI GAAnn'nnTTC Cuts at: 2061 6668 2061 Size: 4607 3932 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6347 276 Size: 53 83 186 936 4813 2468 Fragments arranged by size: 4813 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV PacI PmlI PspXI RsrII SfiI SfoI SrfI SwaI