(Circular) (Six-base) MAPSORT of: pFN21AA1160.seq Check: 3823 from: 1 to: 5698 pFN21AA1160 5698 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3508 278 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3041 1937 Size: 1104 4594 Acc65I G'GTAC_C Cuts at: 3019 3019 Size: 5698 AclI AA'CG_TT Cuts at: 1563 3825 4198 1563 Size: 2262 373 3063 Fragments arranged by size: 3063 2262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3761 5145 5413 1632 Size: 2129 1384 268 1917 Fragments arranged by size: 2129 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 2444 4597 5574 1203 Size: 1241 2153 977 1327 Fragments arranged by size: 2153 1327 1241 977 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 4427 4427 Size: 5698 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5698 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5698 AlwNI CAG_nnn'CTG Cuts at: 3441 5013 3441 Size: 1572 4126 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 2204 2211 3007 3233 3385 5494 1493 Size: 711 7 796 226 152 2109 1697 Fragments arranged by size: 2109 1697 796 711 226 152 7 AseI AT'TA_AT Cuts at: 160 1032 4252 160 Size: 872 3220 1606 Fragments arranged by size: 3220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 3023 1870 Size: 73 1080 4545 Fragments arranged by size: 4545 1080 73 BamHI G'GATC_C Cuts at: 3028 3395 3028 Size: 367 5331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2976 3019 4474 618 Size: 325 217 627 1189 43 1455 1842 Fragments arranged by size: 1842 1455 1189 627 325 217 43 BanII G_rGCy'C Cuts at: 1391 2457 2965 3017 1391 Size: 1066 508 52 4072 Fragments arranged by size: 4072 1066 508 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2095 928 Size: 971 196 4531 Fragments arranged by size: 4531 971 196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 4800 3590 Size: 1210 4488 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2033 2929 4309 Size: 107 122 71 631 605 361 896 1380 Cuts at: 4309 136 Size: 1525 Fragments arranged by size: 1525 1380 896 631 605 361 122 107 71 BglII A'GATC_T Cuts at: 2160 2861 5693 2160 Size: 701 2832 2165 Fragments arranged by size: 2832 2165 701 BlpI GC'TnA_GC Cuts at: 2058 2451 3115 2058 Size: 393 664 4641 Fragments arranged by size: 4641 664 393 Bme1580I G_kGCm'C Cuts at: 1790 3759 4915 1790 Size: 1969 1156 2573 Fragments arranged by size: 2573 1969 1156 BmgBI CAC'GTC Cuts at: 1318 2731 1318 Size: 1413 4285 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2272 3429 4387 Size: 416 53 83 399 24 855 1157 958 Cuts at: 4387 442 Size: 1753 Fragments arranged by size: 1753 1157 958 855 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2604 2604 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2780 4358 1239 Size: 1541 1578 2579 Fragments arranged by size: 2579 1578 1541 Bpu10I CC'TnA_GC Cuts at: 2038 2246 2038 Size: 208 5490 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3823 4688 4986 3823 Size: 865 298 4535 Fragments arranged by size: 4535 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2807 4361 882 Size: 617 1308 1554 2219 Fragments arranged by size: 2219 1554 1308 617 BsaAI yAC'GTr Cuts at: 493 1204 2447 3489 493 Size: 711 1243 1042 2702 Fragments arranged by size: 2702 1243 1042 711 BsaBI GATnn'nnATC Cuts at: 2642 5692 2642 Size: 3050 2648 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3505 3887 4522 275 Size: 53 83 186 936 1972 382 635 1451 Fragments arranged by size: 1972 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2271 4131 4529 4803 4950 5361 2271 Size: 1860 398 274 147 411 2608 Fragments arranged by size: 2608 1860 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5473 Fragments arranged by size: 5473 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2421 2616 2630 2633 2642 2675 2678 2774 Size: 1235 195 14 3 9 33 3 96 Cuts at: 2774 2783 2973 1186 Size: 9 190 3911 Fragments arranged by size: 3911 1235 195 190 96 33 14 9 9 3 3 BseYI C'CCAG_C Cuts at: 2284 4901 5397 2284 Size: 2617 496 2585 Fragments arranged by size: 2617 2585 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5125 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2876 3148 3909 4937 1540 Size: 275 186 875 272 761 1028 2301 Fragments arranged by size: 2301 1028 875 761 275 272 186 BsiHKAI G_wGCw'C Cuts at: 2457 2965 3759 3844 4915 2457 Size: 508 794 85 1071 3240 Fragments arranged by size: 3240 1071 794 508 85 BsmI GAATG_Cn' Cuts at: 2194 3209 3302 2194 Size: 1015 93 4590 Fragments arranged by size: 4590 1015 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2134 2134 Size: 5698 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2457 2965 3017 3759 3844 4915 1391 Size: 399 667 508 52 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 667 508 399 85 52 BspEI T'CCGG_A Cuts at: 2271 5361 2271 Size: 3090 2608 BspHI T'CATG_A Cuts at: 2329 2457 3587 5317 2329 Size: 128 1130 1730 2710 Fragments arranged by size: 2710 1730 1130 128 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3053 844 Size: 2209 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2886 3585 1394 Size: 429 1063 699 3507 Fragments arranged by size: 3507 1063 699 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2721 4193 4367 65 Size: 1183 1473 1472 174 1396 Fragments arranged by size: 1473 1472 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2875 4342 4529 1700 Size: 110 1065 1467 187 2869 Fragments arranged by size: 2869 1467 1065 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5698 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5698 BssSI C'ACGA_G Cuts at: 3758 4770 3758 Size: 1012 4686 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5698 BstBI TT'CG_AA Cuts at: 2209 4502 2209 Size: 2293 3405 BstEII G'GTnAC_C Cuts at: 1184 2486 1184 Size: 1302 4396 BstYI r'GATC_y Cuts at: 1972 2160 2861 3028 3395 3780 3797 5249 5335 Size: 188 701 167 367 385 17 1452 86 Cuts at: 5335 5347 5693 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 701 385 367 346 188 167 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4207 Fragments arranged by size: 4207 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4959 Fragments arranged by size: 4959 683 56 BtsI GCAGTG_nn' Cuts at: 2925 3214 4008 4028 2925 Size: 289 794 20 4595 Fragments arranged by size: 4595 794 289 20 ClaI AT'CG_AT Cuts at: 1554 2469 1554 Size: 915 4783 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 3002 3002 Size: 5698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4705 809 Size: 507 3389 1802 Fragments arranged by size: 3389 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2873 2877 3145 Size: 54 1425 50 275 18 1043 4 268 Cuts at: 3145 4034 5663 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1043 889 275 268 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2873 3145 1537 Size: 275 1061 272 4090 Fragments arranged by size: 4090 1061 275 272 EarI CTCTTCn'nnn_ Cuts at: 1353 2061 2430 2607 2619 2628 3628 1353 Size: 708 369 177 12 9 1000 3423 Fragments arranged by size: 3423 1000 708 369 177 12 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4285 4659 4805 387 Size: 1064 138 2696 374 146 1280 Fragments arranged by size: 2696 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2455 2963 2455 Size: 508 5190 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2780 3761 4358 5145 5413 1239 Size: 393 1148 981 597 787 268 1524 Fragments arranged by size: 1524 1148 981 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2291 2291 Size: 5698 EcoRI G'AATT_C Cuts at: 2211 3007 3385 2211 Size: 796 378 4524 Fragments arranged by size: 4524 796 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2441 2441 Size: 5698 FseI GG_CCGG'CC Cuts at: 2879 2879 Size: 5698 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 4845 1678 Size: 3167 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2133 2165 2651 2683 4339 Size: 32 135 32 561 32 486 32 1656 Cuts at: 4339 4371 4413 4445 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1656 561 486 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3042 3288 669 Size: 988 281 1104 246 3079 Fragments arranged by size: 3079 1104 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5698 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KpnI G_GTAC'C Cuts at: 3023 3023 Size: 5698 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2649 4812 4996 1305 Size: 1344 2163 184 2007 Fragments arranged by size: 2163 2007 1344 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3876 Fragments arranged by size: 3876 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3656 4015 4174 518 Size: 648 2490 359 159 2042 Fragments arranged by size: 2490 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2062 3114 3791 4939 5184 1606 Size: 456 1052 677 1148 245 2120 Fragments arranged by size: 2120 1148 1052 677 456 245 NaeI GCC'GGC Cuts at: 2877 2877 Size: 5698 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4207 Fragments arranged by size: 4207 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5698 NgoMIV G'CCGG_C Cuts at: 2875 2875 Size: 5698 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 3145 3145 Size: 5698 NspI r_CATG'y Cuts at: 1132 1229 3056 4601 1132 Size: 97 1827 1545 2229 Fragments arranged by size: 2229 1827 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5698 PciI A'CATG_T Cuts at: 4597 4597 Size: 5698 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5698 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PmlI CAC'GTG Cuts at: 2447 2447 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3767 3799 5280 5312 3767 Size: 32 1481 32 4153 Fragments arranged by size: 4153 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2291 2291 Size: 5698 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5698 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PstI C_TGCA'G Cuts at: 830 2823 2861 3050 830 Size: 1993 38 189 3478 Fragments arranged by size: 3478 1993 189 38 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 PvuII CAG'CTG Cuts at: 2062 2062 Size: 5698 SacI G_AGCT'C Cuts at: 2457 2965 2457 Size: 508 5190 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SbfI CC_TGCA'GG Cuts at: 2823 3050 2823 Size: 227 5471 ScaI AGT'ACT Cuts at: 2369 2480 3946 5658 2369 Size: 111 1466 1712 2409 Fragments arranged by size: 2409 1712 1466 111 SfcI C'TryA_G Cuts at: 826 1045 2819 2857 3046 4181 4862 5053 826 Size: 219 1774 38 189 1135 681 191 1471 Fragments arranged by size: 1774 1471 1135 681 219 191 189 38 SfiI GGCCn_nnn'nGGCC Cuts at: 2033 2033 Size: 5698 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 3025 1872 Size: 1153 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3802 4703 4965 820 Size: 197 926 1859 901 262 1553 Fragments arranged by size: 1859 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3489 493 Size: 2996 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 3056 1132 Size: 1924 3774 SspI AAT'ATT Cuts at: 5 52 3622 5 Size: 47 3570 2081 Fragments arranged by size: 3570 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5698 StyI C'CwwG_G Cuts at: 513 1065 2004 3137 513 Size: 552 939 1133 3074 Fragments arranged by size: 3074 1133 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2917 3725 3742 3895 4080 4525 325 Size: 1255 1337 808 17 153 185 445 1498 Fragments arranged by size: 1498 1337 1255 808 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2111 2367 2478 Size: 274 80 33 51 1443 134 256 111 Cuts at: 2478 3944 5656 96 Size: 1466 1712 138 Fragments arranged by size: 1712 1466 1443 274 256 138 134 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5658 5690 5658 Size: 32 5666 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5698 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2848 2848 Size: 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 3023 1870 Size: 1153 4545 XmnI GAAnn'nnTTC Cuts at: 2424 3827 2424 Size: 1403 4295 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3506 276 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI