(Circular) (Six-base) MAPSORT of: pFN21AA1141.seq Check: 1571 from: 1 to: 7318 pFN21AA1141 7318 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5128 278 Size: 53 83 186 936 3592 2468 Fragments arranged by size: 3592 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4661 1937 Size: 2724 4594 Acc65I G'GTAC_C Cuts at: 4639 4639 Size: 7318 AclI AA'CG_TT Cuts at: 1563 4133 4540 5445 5818 1563 Size: 2570 407 905 373 3063 Fragments arranged by size: 3063 2570 905 407 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2641 3223 3614 3896 3952 5381 6765 7033 Size: 1009 582 391 282 56 1429 1384 268 Cuts at: 7033 1632 Size: 1917 Fragments arranged by size: 1917 1429 1384 1009 582 391 282 268 56 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7121 AflIII A'CryG_T Cuts at: 1203 2091 6217 7194 1203 Size: 888 4126 977 1327 Fragments arranged by size: 4126 1327 977 888 AgeI A'CCGG_T Cuts at: 6149 6149 Size: 7318 AhdI GACnn_n'nnGTC Cuts at: 6047 6047 Size: 7318 AleI CACnn'nnGTG Cuts at: 1166 3606 4179 1166 Size: 2440 573 4305 Fragments arranged by size: 4305 2440 573 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7318 AlwNI CAG_nnn'CTG Cuts at: 4501 5061 6633 4501 Size: 560 1572 5186 Fragments arranged by size: 5186 1572 560 ApaLI G'TGCA_C Cuts at: 3978 5375 6531 3978 Size: 1397 1156 4765 Fragments arranged by size: 4765 1397 1156 ApoI r'AATT_y Cuts at: 1493 2015 2792 3357 4170 4254 4422 4627 4853 Size: 522 777 565 813 84 168 205 226 Cuts at: 4853 5005 7114 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 813 777 565 522 226 205 168 152 84 AseI AT'TA_AT Cuts at: 160 1032 5872 160 Size: 872 4840 1606 Fragments arranged by size: 4840 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7318 AvaI C'yCGr_G Cuts at: 1870 1943 2072 4567 4643 1870 Size: 73 129 2495 76 4545 Fragments arranged by size: 4545 2495 129 76 73 BamHI G'GATC_C Cuts at: 4648 5015 4648 Size: 367 6951 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3015 4560 4639 6094 618 Size: 325 217 627 1228 1545 79 1455 1842 Fragments arranged by size: 1842 1545 1455 1228 627 325 217 79 BanII G_rGCy'C Cuts at: 1391 4148 4316 4637 1391 Size: 2757 168 321 4072 Fragments arranged by size: 4072 2757 321 168 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2390 4042 928 Size: 971 491 1652 4204 Fragments arranged by size: 4204 1652 971 491 BbvCI CC'TCA_GC Cuts at: 2010 4574 2010 Size: 2564 4754 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5509 5543 1903 Size: 34 3572 34 3678 Fragments arranged by size: 3678 3572 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2778 5210 6420 2778 Size: 2432 1210 3676 Fragments arranged by size: 3676 2432 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7318 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5929 136 Size: 107 122 71 631 605 4257 1525 Fragments arranged by size: 4257 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2185 3651 7313 2185 Size: 1466 3662 2190 Fragments arranged by size: 3662 2190 1466 BlpI GC'TnA_GC Cuts at: 4233 4735 4233 Size: 502 6816 Bme1580I G_kGCm'C Cuts at: 1790 3982 4486 5379 6535 1790 Size: 2192 504 893 1156 2573 Fragments arranged by size: 2573 2192 1156 893 504 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7318 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2071 2901 4356 Size: 416 53 83 399 24 654 830 1455 Cuts at: 4356 5049 6007 442 Size: 693 958 1753 Fragments arranged by size: 1753 1455 958 830 693 654 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7318 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2593 2593 Size: 7318 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2886 3219 4032 4284 5978 1239 Size: 971 676 333 813 252 1694 2579 Fragments arranged by size: 2579 1694 971 813 676 333 252 Bpu10I CC'TnA_GC Cuts at: 2010 4574 2010 Size: 2564 4754 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2012 3097 5443 6308 6606 2012 Size: 1085 2346 865 298 2724 Fragments arranged by size: 2724 2346 1085 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2234 5981 882 Size: 617 735 3747 2219 Fragments arranged by size: 3747 2219 735 617 BsaAI yAC'GTr Cuts at: 493 1204 5109 493 Size: 711 3905 2702 Fragments arranged by size: 3905 2702 711 BsaBI GATnn'nnATC Cuts at: 3501 7312 3501 Size: 3811 3507 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5125 5507 6142 275 Size: 53 83 186 936 3592 382 635 1451 Fragments arranged by size: 3592 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2745 4332 5751 6149 6423 6570 6981 2745 Size: 1587 1419 398 274 147 411 3082 Fragments arranged by size: 3082 1587 1419 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2147 2177 2656 2686 1178 Size: 30 165 30 744 30 479 30 5810 Fragments arranged by size: 5810 744 479 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2244 3218 1186 Size: 1058 974 5286 Fragments arranged by size: 5286 1058 974 BseYI C'CCAG_C Cuts at: 2679 6521 7017 2679 Size: 3842 496 2980 Fragments arranged by size: 3842 2980 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6745 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4768 5529 6557 1540 Size: 275 186 2767 761 1028 2301 Fragments arranged by size: 2767 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3936 3982 4148 4316 5379 5464 6535 3936 Size: 46 166 168 1063 85 1071 4719 Fragments arranged by size: 4719 1071 1063 168 166 85 46 BsmI GAATG_Cn' Cuts at: 2447 2755 2981 3398 3482 3959 4127 4295 4829 Size: 308 226 417 84 477 168 168 534 Cuts at: 4829 4922 2447 Size: 93 4843 Fragments arranged by size: 4843 534 477 417 308 226 168 168 93 84 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3936 3982 4148 4316 4486 4637 5379 Size: 399 2146 46 166 168 170 151 742 Cuts at: 5379 5464 6535 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 2146 1071 742 399 170 168 166 151 85 46 BspEI T'CCGG_A Cuts at: 6981 6981 Size: 7318 BspHI T'CATG_A Cuts at: 2452 2911 5207 6937 2452 Size: 459 2296 1730 2833 Fragments arranged by size: 2833 2296 1730 459 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4673 844 Size: 3829 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5205 1394 Size: 429 3382 3507 Fragments arranged by size: 3507 3382 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5813 5987 65 Size: 1183 4565 174 1396 Fragments arranged by size: 4565 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3327 3411 5962 6149 1700 Size: 110 1517 84 2551 187 2869 Fragments arranged by size: 2869 2551 1517 187 110 84 BsrGI T'GTAC_A Cuts at: 96 4591 96 Size: 4495 2823 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7318 BssSI C'ACGA_G Cuts at: 3602 3743 5378 6390 3602 Size: 141 1635 1012 4530 Fragments arranged by size: 4530 1635 1012 141 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7318 BstBI TT'CG_AA Cuts at: 2274 6122 2274 Size: 3848 3470 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7318 BstXI CCAn_nnnn'nTGG Cuts at: 2174 2174 Size: 7318 BstYI r'GATC_y Cuts at: 1972 2185 2997 3024 3261 3651 4648 5015 5400 Size: 213 812 27 237 390 997 367 385 Cuts at: 5400 5417 6869 6955 6967 7313 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 997 812 390 385 367 346 237 213 86 27 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2436 2493 2508 513 Size: 552 939 432 57 15 5323 Fragments arranged by size: 5323 939 552 432 57 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6579 Fragments arranged by size: 6579 683 56 BtsI GCAGTG_nn' Cuts at: 2185 3310 3898 4834 5628 5648 2185 Size: 1125 588 936 794 20 3855 Fragments arranged by size: 3855 1125 936 794 588 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7318 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7283 DraI TTT'AAA Cuts at: 3943 4027 4622 3943 Size: 84 595 6639 Fragments arranged by size: 6639 595 84 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6325 809 Size: 507 5009 1802 Fragments arranged by size: 5009 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3805 4765 5654 Size: 54 1425 50 275 18 1975 960 889 Cuts at: 5654 7283 8 Size: 1629 43 Fragments arranged by size: 1975 1629 1425 960 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4765 1537 Size: 275 2953 4090 Fragments arranged by size: 4090 2953 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2135 3195 5248 1353 Size: 782 1060 2053 3423 Fragments arranged by size: 3423 2053 1060 782 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4232 5905 6279 6425 387 Size: 1064 138 2643 1673 374 146 1280 Fragments arranged by size: 2643 1673 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4146 4314 4146 Size: 168 7150 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2641 2886 3219 3223 3614 3896 Size: 393 578 431 245 333 4 391 282 Cuts at: 3896 3952 4032 4284 5381 5978 6765 7033 1239 Size: 56 80 252 1097 597 787 268 1524 Fragments arranged by size: 1524 1097 787 597 578 431 393 391 333 282 268 252 245 80 56 4 EcoNI CCTnn'n_nnAGG Cuts at: 2199 2199 Size: 7318 EcoO109I rG'GnC_Cy Cuts at: 2125 2125 Size: 7318 EcoRI G'AATT_C Cuts at: 3357 4170 4254 4422 4627 5005 3357 Size: 813 84 168 205 378 5670 Fragments arranged by size: 5670 813 378 205 168 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2338 2338 Size: 7318 FspI TGC'GCA Cuts at: 5824 5824 Size: 7318 HaeII r_GCGC'y Cuts at: 1678 6465 1678 Size: 4787 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2294 2326 2656 2688 2942 Size: 32 135 32 722 32 330 32 254 Cuts at: 2942 2974 3016 3048 3418 3450 3643 3675 4513 Size: 32 42 32 370 32 193 32 838 Cuts at: 4513 4545 5959 5991 6033 6065 1373 Size: 32 1414 32 42 32 2626 Fragments arranged by size: 2626 1414 838 722 370 330 254 193 135 42 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4538 4662 4908 669 Size: 988 281 2600 124 246 3079 Fragments arranged by size: 3079 2600 988 281 246 124 HindIII A'AGCT_T Cuts at: 748 2653 4467 748 Size: 1905 1814 3599 Fragments arranged by size: 3599 1905 1814 HpaI GTT'AAC Cuts at: 4908 4908 Size: 7318 KpnI G_GTAC'C Cuts at: 4643 4643 Size: 7318 MfeI C'AATT_G Cuts at: 2116 4917 2116 Size: 2801 4517 MluI A'CGCG_T Cuts at: 7194 7194 Size: 7318 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 2610 3118 6432 6616 1305 Size: 786 519 508 3314 184 2007 Fragments arranged by size: 3314 2007 786 519 508 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3807 10 Size: 54 1425 343 1975 3521 Fragments arranged by size: 3521 1975 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2172 2632 2842 3342 3550 3606 4179 Size: 648 1006 460 210 500 208 56 573 Cuts at: 4179 4587 5276 5635 5794 518 Size: 408 689 359 159 2042 Fragments arranged by size: 2042 1006 689 648 573 500 460 408 359 210 208 159 56 MspA1I CmG'CkG Cuts at: 1606 4734 5411 6559 6804 1606 Size: 3128 677 1148 245 2120 Fragments arranged by size: 3128 2120 1148 677 245 NaeI GCC'GGC Cuts at: 3413 3413 Size: 7318 NcoI C'CATG_G Cuts at: 513 1065 2004 2508 513 Size: 552 939 504 5323 Fragments arranged by size: 5323 939 552 504 NdeI CA'TA_TG Cuts at: 387 3547 387 Size: 3160 4158 NgoMIV G'CCGG_C Cuts at: 3411 3411 Size: 7318 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7318 NotI GC'GGCC_GC Cuts at: 4765 4765 Size: 7318 NspI r_CATG'y Cuts at: 1132 1229 4676 6221 1132 Size: 97 3447 1545 2229 Fragments arranged by size: 3447 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7318 PciI A'CATG_T Cuts at: 6217 6217 Size: 7318 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7318 PmeI GTTT'AAAC Cuts at: 4622 4622 Size: 7318 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5387 5419 6900 6932 5387 Size: 32 1481 32 5773 Fragments arranged by size: 5773 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 2125 Size: 7318 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7318 PsiI TTA'TAA Cuts at: 3213 4888 3213 Size: 1675 5643 PspXI vC'TCGA_Gb Cuts at: 4567 4567 Size: 7318 PstI C_TGCA'G Cuts at: 830 3725 4670 830 Size: 2895 945 3478 Fragments arranged by size: 3478 2895 945 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7318 SacI G_AGCT'C Cuts at: 4148 4316 4148 Size: 168 7150 SalI G'TCGA_C Cuts at: 1936 4660 1936 Size: 2724 4594 SapI GCTCTTCn'nnn_ Cuts at: 3195 3195 Size: 7318 SbfI CC_TGCA'GG Cuts at: 4670 4670 Size: 7318 ScaI AGT'ACT Cuts at: 3814 5566 7278 3814 Size: 1752 1712 3854 Fragments arranged by size: 3854 1752 1712 SexAI A'CCwGG_T Cuts at: 2363 2363 Size: 7318 SfcI C'TryA_G Cuts at: 826 1045 3370 3463 3721 4360 4666 5801 6482 Size: 219 2325 93 258 639 306 1135 681 Cuts at: 6482 6673 826 Size: 191 1471 Fragments arranged by size: 2325 1471 1135 681 639 306 258 219 191 93 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7318 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7318 SmaI CCC'GGG Cuts at: 1872 4645 1872 Size: 2773 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2027 3076 4567 5422 6323 6585 Size: 197 926 84 1049 1491 855 901 262 Cuts at: 6585 820 Size: 1553 Fragments arranged by size: 1553 1491 1049 926 901 855 262 197 84 SnaBI TAC'GTA Cuts at: 493 5109 493 Size: 4616 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7318 SphI G_CATG'C Cuts at: 1132 4676 1132 Size: 3544 3774 SspI AAT'ATT Cuts at: 5 52 5242 5 Size: 47 5190 2081 Fragments arranged by size: 5190 2081 47 StuI AGG'CCT Cuts at: 1517 3320 4217 1517 Size: 1803 897 4618 Fragments arranged by size: 4618 1803 897 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 2313 2508 4757 513 Size: 552 939 48 261 195 2249 3074 Fragments arranged by size: 3074 2249 939 552 261 195 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2184 2916 2920 4070 4322 4529 5345 Size: 1255 604 732 4 1150 252 207 816 Cuts at: 5345 5362 5515 5700 6145 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1150 816 732 604 445 252 207 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2834 3812 4591 Size: 274 80 33 51 1443 857 978 779 Cuts at: 4591 5564 7276 96 Size: 973 1712 138 Fragments arranged by size: 1712 1443 978 973 857 779 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3624 3656 7278 7310 3624 Size: 32 3622 32 3632 Fragments arranged by size: 3632 3622 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2472 4046 1316 Size: 1156 1574 4588 Fragments arranged by size: 4588 1574 1156 XbaI T'CTAG_A Cuts at: 3845 4654 3845 Size: 809 6509 XhoI C'TCGA_G Cuts at: 1943 4567 1943 Size: 2624 4694 XmaI C'CCGG_G Cuts at: 1870 4643 1870 Size: 2773 4545 XmnI GAAnn'nnTTC Cuts at: 2397 2654 3236 3320 4133 4217 4301 5447 2397 Size: 257 582 84 813 84 84 1146 4268 Fragments arranged by size: 4268 1146 813 582 257 84 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5126 276 Size: 53 83 186 936 3592 2468 Fragments arranged by size: 3592 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI