Restriction map for pFN21AA1124
 (Circular) (Six-base) MAPSORT of: pFN21AA1124.seq  Check: 2147  from: 1  to: 9838



pFN21AA1124  9838 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   6038    6799    6038 
   Size:        761    9077
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    6462    7648     278 
   Size:         53      83     186     936    4926    1186    2468

  Fragments arranged by size: 

                4926    2468    1186     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    7181    1937 
   Size:       5244    4594
 
Acc65I G'GTAC_C

Cuts at:   7159    7159 
   Size:       9838
 
AclI AA'CG_TT

Cuts at:   1563    7965    8338    1563 
   Size:       6402     373    3063

  Fragments arranged by size: 

                6402    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2955    3269    5421    6310    6549    7901    9285    9553 
   Size:       1323     314    2152     889     239    1352    1384     268

Cuts at:   9553    1632 
   Size:       1917

  Fragments arranged by size: 

                2152    1917    1384    1352    1323     889     314     268
                 239
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    9641
 
AflIII A'CryG_T

Cuts at:   1203    4620    5196    8737    9714    1203 
   Size:       3417     576    3541     977    1327

  Fragments arranged by size: 

                3541    3417    1327     977     576
 
AgeI A'CCGG_T

Cuts at:   8669    8669 
   Size:       9838
 
AhdI GACnn_n'nnGTC

Cuts at:   2797    8567    2797 
   Size:       5770    4068
 
AleI CACnn'nnGTG

Cuts at:   1166    5330    1166 
   Size:       4164    5674
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3401    6827    1184 
   Size:       2217    3426    4195

  Fragments arranged by size: 

                4195    3426    2217
 
AlwNI CAG_nnn'CTG

Cuts at:   2962    3586    4414    4615    4823    5050    5127    6139    6162 
   Size:        624     828     201     208     227      77    1012      23

Cuts at:   6162    6646    7581    9153    2962 
   Size:        484     935    1572    3647

  Fragments arranged by size: 

                3647    1572    1012     935     828     624     484     227
                 208     201      77      23
 
ApaI G_GGCC'C

Cuts at:   2058    3436    6794    6869    2058 
   Size:       1378    3358      75    5027

  Fragments arranged by size: 

                5027    3358    1378      75
 
ApaLI G'TGCA_C

Cuts at:   2594    5556    5912    7895    9051    2594 
   Size:       2962     356    1983    1156    3381

  Fragments arranged by size: 

                3381    2962    1983    1156     356
 
ApoI r'AATT_y

Cuts at:   1493    2304    2504    4782    4808    7147    7373    7525    9634 
   Size:        811     200    2278      26    2339     226     152    2109

Cuts at:   9634    1493 
   Size:       1697

  Fragments arranged by size: 

                2339    2278    2109    1697     811     226     200     152
                  26
 
AseI AT'TA_AT

Cuts at:    160    1032    8392     160 
   Size:        872    7360    1606

  Fragments arranged by size: 

                7360    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       9838
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2171    3429    3524    4497    4541    5240    5808 
   Size:         73     228    1258      95     973      44     699     568

Cuts at:   5808    6592    7163    1870 
   Size:        784     571    4545

  Fragments arranged by size: 

                4545    1258     973     784     699     571     568     228
                  95      73      44
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2913    2946    2913 
   Size:         33    9805
 
BamHI G'GATC_C

Cuts at:   7168    7535    7168 
   Size:        367    9471
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    4025    5213    6026    6533    7159 
   Size:        325     217     627    2238    1188     813     507     626

Cuts at:   7159    8614     618 
   Size:       1455    1842

  Fragments arranged by size: 

                2238    1842    1455    1188     813     627     626     507
                 325     217
 
BanII G_rGCy'C

Cuts at:   1391    2058    3436    3765    4542    4695    4830    5477    5790 
   Size:        667    1378     329     777     153     135     647     313

Cuts at:   5790    6300    6384    6794    6869    7076    7157    1391 
   Size:        510      84     410      75     207      81    4072

  Fragments arranged by size: 

                4072    1378     777     667     647     510     410     329
                 313     207     153     135      84      81      75
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2232    2517    6667     928 
   Size:        971     333     285    4150    4099

  Fragments arranged by size: 

                4150    4099     971     333     285
 
BbvCI CC'TCA_GC

Cuts at:   3437    3918    5666    3437 
   Size:        481    1748    7609

  Fragments arranged by size: 

                7609    1748     481
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2201    2235    4475    4509    8029    8063    1903 
   Size:         34     264      34    2240      34    3520      34    3678

  Fragments arranged by size: 

                3678    3520    2240     264      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   7730    8940    7730 
   Size:       1210    8628
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       9838
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    4655    8449 
   Size:        107     122      71     631     605     337    2646    3794

Cuts at:   8449     136 
   Size:       1525

  Fragments arranged by size: 

                3794    2646    1525     631     605     337     122     107
                  71
 
BglII A'GATC_T

Cuts at:   9833    9833 
   Size:       9838
 
BlpI GC'TnA_GC

Cuts at:   2498    3374    3897    4912    6194    7255    2498 
   Size:        876     523    1015    1282    1061    5081

  Fragments arranged by size: 

                5081    1282    1061    1015     876     523
 
Bme1580I G_kGCm'C

Cuts at:   1790    2058    2598    2720    3436    3772    5218    5231    5560 
   Size:        268     540     122     716     336    1446      13     329

Cuts at:   5560    5916    6370    6794    6843    6869    7899    9055    1790 
   Size:        356     454     424      49      26    1030    1156    2573

  Fragments arranged by size: 

                2573    1446    1156    1030     716     540     454     424
                 356     336     329     268     122      49      26      13
            
 
BmgBI CAC'GTC

Cuts at:   1318    2101    2632    3118    3130    4115    5272    6430    1318 
   Size:        783     531     486      12     985    1157    1158    4726

  Fragments arranged by size: 

                4726    1158    1157     985     783     531     486      12
            
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4453    4833    7569 
   Size:        416      53      83     399      24    3036     380    2736

Cuts at:   7569    8527     442 
   Size:        958    1753

  Fragments arranged by size: 

                3036    2736    1753     958     416     399     380      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       9838
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   6620    6620 
   Size:       9838
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2054    3326    3389    3617    4007    4094    4415    4655 
   Size:        815    1272      63     228     390      87     321     240

Cuts at:   4655    4673    5089    5624    7136    8498    1239 
   Size:         18     416     535    1512    1362    2579

  Fragments arranged by size: 

                2579    1512    1362    1272     815     535     416     390
                 321     240     228      87      63      18
 
Bpu10I CC'TnA_GC

Cuts at:   2078    3437    3878    3918    4694    5666    6149    2078 
   Size:       1359     441      40     776     972     483    5767

  Fragments arranged by size: 

                5767    1359     972     776     483     441      40
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2009    3557    3980    5996    7963    8828    9126    2009 
   Size:       1548     423    2016    1967     865     298    2721

  Fragments arranged by size: 

                2721    2016    1967    1548     865     423     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2348    2805    2861    6720    8501     882 
   Size:        617     849     457      56    3859    1781    2219

  Fragments arranged by size: 

                3859    2219    1781     849     617     457      56
 
BsaAI yAC'GTr

Cuts at:    493    1204    2158    2593    5197    6346    6778    7629     493 
   Size:        711     954     435    2604    1149     432     851    2702

  Fragments arranged by size: 

                2702    2604    1149     954     851     711     435     432
            
 
BsaBI GATnn'nnATC

Cuts at:   3702    5882    9832    3702 
   Size:       2180    3950    3708

  Fragments arranged by size: 

                3950    3708    2180
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3843    4710    6459    7645 
   Size:         53      83     186     936    2310     867    1749    1186

Cuts at:   7645    8027    8662     275 
   Size:        382     635    1451

  Fragments arranged by size: 

                2310    1749    1451    1186     936     867     635     382
                 186      83      53
 
BsaWI w'CCGG_w

Cuts at:   2521    5971    8271    8669    8943    9090    9501    2521 
   Size:       3450    2300     398     274     147     411    2858

  Fragments arranged by size: 

                3450    2858    2300     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    4546    4576    4628    4658    4921 
   Size:         30     165      30    3143      30      52      30     263

Cuts at:   4921    4951    5117    5147    6371    6401    1178 
   Size:         30     166      30    1224      30    4615

  Fragments arranged by size: 

                4615    3143    1224     263     166     165      52      30
                  30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2387    3352    3647    3824    3827    4274    4567    4703 
   Size:       1201     965     295     177       3     447     293     136

Cuts at:   4703    4869    5879    6323    6536    1186 
   Size:        166    1010     444     213    4488

  Fragments arranged by size: 

                4488    1201    1010     965     447     444     295     293
                 213     177     166     136       3
 
BseYI C'CCAG_C

Cuts at:   2986    5415    7058    9041    9537    2986 
   Size:       2429    1643    1983     496    3287

  Fragments arranged by size: 

                3287    2429    1983    1643     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2146    2405    2717    3314    3428    3623    5113 
   Size:        573     410     259     312     597     114     195    1490

Cuts at:   5113    5140    5294    6152    6509    1163 
   Size:         27     154     858     357    4492

  Fragments arranged by size: 

                4492    1490     858     597     573     410     357     312
                 259     195     154     114      27
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    7288    8049    9077    1540 
   Size:        275     186    5287     761    1028    2301

  Fragments arranged by size: 

                5287    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2106    2598    3312    3765    3951    4041    4542    4695    5477 
   Size:        492     714     453     186      90     501     153     782

Cuts at:   5477    5560    5916    6150    6300    6384    6816    7899    7984 
   Size:         83     356     234     150      84     432    1083      85

Cuts at:   7984    9055    2106 
   Size:       1071    2889

  Fragments arranged by size: 

                2889    1083    1071     782     714     501     492     453
                 432     356     234     186     153     150      90      85
                  84      83
 
BsmI GAATG_Cn'

Cuts at:   7349    7442    7349 
   Size:         93    9745
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2058    2106    2598    2720    3312    3436    3765 
   Size:        399     268      48     492     122     592     124     329

Cuts at:   3765    3772    3951    4041    4542    4695    4830    5218    5231 
   Size:          7     179      90     501     153     135     388      13

Cuts at:   5231    5477    5560    5790    5916    6150    6300    6370    6384 
   Size:        246      83     230     126     234     150      70      14

Cuts at:   6384    6794    6816    6843    6869    7076    7157    7899    7984 
   Size:        410      22      27      26     207      81     742      85

Cuts at:   7984    9055    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     592     501     492     410     399
                 388     329     268     246     234     230     207     179
                 153     150     135     126     124     122      90      85
                  83      81      70      48      27      26      22      14
                  13       7
 
BspEI T'CCGG_A

Cuts at:   2521    5971    9501    2521 
   Size:       3450    3530    2858

  Fragments arranged by size: 

                3530    3450    2858
 
BspHI T'CATG_A

Cuts at:   2883    7727    9457    2883 
   Size:       4844    1730    3264

  Fragments arranged by size: 

                4844    3264    1730
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    6038    6324    6799    6831    7193     844 
   Size:       5194     286     475      32     362    3489

  Fragments arranged by size: 

                5194    3489     475     362     286      32
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3667    4022    5945    6599    6872    7725    1394 
   Size:        429    1844     355    1923     654     273     853    3507

  Fragments arranged by size: 

                3507    1923    1844     853     654     429     355     273
            
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2319    5427    8333    8507      65 
   Size:       1183    1071    3108    2906     174    1396

  Fragments arranged by size: 

                3108    2906    1396    1183    1071     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2982    5661    7123    8482    8669    1700 
   Size:        110    1172    2679    1462    1359     187    2869

  Fragments arranged by size: 

                2869    2679    1462    1359    1172     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2115    6438      96 
   Size:       2019    4323    3496

  Fragments arranged by size: 

                4323    3496    2019
 
BssHII G'CGCG_C

Cuts at:   1925    5350    6372    1925 
   Size:       3425    1022    5391

  Fragments arranged by size: 

                5391    3425    1022
 
BssSI C'ACGA_G

Cuts at:   2861    6922    7898    8910    2861 
   Size:       4061     976    1012    3789

  Fragments arranged by size: 

                4061    3789    1012     976
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3316    3586    3859    5127    6646     794 
   Size:       2522     270     273    1268    1519    3986

  Fragments arranged by size: 

                3986    2522    1519    1268     273     270
 
BstBI TT'CG_AA

Cuts at:   8642    8642 
   Size:       9838
 
BstEII G'GTnAC_C

Cuts at:   1184    4502    5803    1184 
   Size:       3318    1301    5219

  Fragments arranged by size: 

                5219    3318    1301
 
BstXI CCAn_nnnn'nTGG

Cuts at:   6883    6883 
   Size:       9838
 
BstYI r'GATC_y

Cuts at:   1972    2742    4411    4662    5446    6072    6740    7168    7535 
   Size:        770    1669     251     784     626     668     428     367

Cuts at:   7535    7920    7937    9389    9475    9487    9833    1972 
   Size:        385      17    1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1669    1452     784     770     668     626     428
                 385     367     346     251      86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2706    2712    3418    5325    5924    6000    6477 
   Size:        552    1641       6     706    1907     599      76     477

Cuts at:   6477     513 
   Size:       3874

  Fragments arranged by size: 

                3874    1907    1641     706     599     552     477      76
                   6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2394    2768    4319     487 
   Size:        683      56    1168     374    1551    6006

  Fragments arranged by size: 

                6006    1551    1168     683     374      56
 
BtsI GCAGTG_nn'

Cuts at:   4872    5125    6140    7354    8148    8168    4872 
   Size:        253    1015    1214     794      20    6542

  Fragments arranged by size: 

                6542    1214    1015     794     253      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       9838
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    9803
 
DraI TTT'AAA

Cuts at:   7142    7142 
   Size:       9838
 
DraIII CAC_nnn'GTG

Cuts at:   2124    3613    5911    2124 
   Size:       1489    2298    6051

  Fragments arranged by size: 

                6051    2298    1489
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2105    3409    8845     809 
   Size:        507     789    1304    5436    1802

  Fragments arranged by size: 

                5436    1802    1304     789     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2010    2160    2562 
   Size:         54    1425      50     275      18     180     150     402

Cuts at:   2562    2709    3825    5896    5997    6003    6042    7285    8174 
   Size:        147    1116    2071     101       6      39    1243     889

Cuts at:   8174    9803       8 
   Size:       1629      43

  Fragments arranged by size: 

                2071    1629    1425    1243    1116     889     402     275
                 180     150     147     101      54      50      43      39
                  18       6
 
EagI C'GGCC_G

Cuts at:   1537    1812    7285    1537 
   Size:        275    5473    4090

  Fragments arranged by size: 

                5473    4090     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2248    2889    3245    4092    4530    5387    6687    7768 
   Size:        895     641     356     847     438     857    1300    1081

Cuts at:   7768    1353 
   Size:       3423

  Fragments arranged by size: 

                3423    1300    1081     895     857     847     641     438
                 356
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2636    4673    8425    8799    8945     387 
   Size:       1064     138    1047    2037    3752     374     146    1280

  Fragments arranged by size: 

                3752    2037    1280    1064    1047     374     146     138
            
 
EcoICRI GAG'CTC

Cuts at:   3763    4540    4693    5475    6298    6382    3763 
   Size:        777     153     782     823      84    7219

  Fragments arranged by size: 

                7219     823     782     777     153      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2054    2955    3269    3326    3389    3617    4007 
   Size:        393     422     901     314      57      63     228     390

Cuts at:   4007    4094    4415    4655    4673    5089    5421    5624    6310 
   Size:         87     321     240      18     416     332     203     686

Cuts at:   6310    6549    7136    7901    8498    9285    9553    1239 
   Size:        239     587     765     597     787     268    1524

  Fragments arranged by size: 

                1524     901     787     765     686     597     587     422
                 416     393     390     332     321     314     268     240
                 239     228     203      87      63      57      18
 
EcoO109I rG'GnC_Cy

Cuts at:   2055    2947    3301    3433    3991    5047    5314    6516    6682 
   Size:        892     354     132     558    1056     267    1202     166

Cuts at:   6682    6866    2055 
   Size:        184    5027

  Fragments arranged by size: 

                5027    1202    1056     892     558     354     267     184
                 166     132
 
EcoRI G'AATT_C

Cuts at:   7147    7525    7147 
   Size:        378    9460
 
EcoRV GAT'ATC

Cuts at:   6753    6753 
   Size:       9838
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2277    2534    3917    4102    2277 
   Size:        257    1383     185    8013

  Fragments arranged by size: 

                8013    1383     257     185
 
FspI TGC'GCA

Cuts at:   6473    6650    8344    6473 
   Size:        177    1694    7967

  Fragments arranged by size: 

                7967    1694     177
 
FspAI rTGC'GCAy

Cuts at:   6473    6473 
   Size:       9838
 
HaeII r_GCGC'y

Cuts at:   1678    2076    3554    8985    1678 
   Size:        398    1478    5431    2531

  Fragments arranged by size: 

                5431    2531    1478     398
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2648    2680    2717    2749    2783 
   Size:         32     135      32    1076      32      37      32      34

Cuts at:   2783    2815    3227    3259    4546    4578    4628    4660    5530 
   Size:         32     412      32    1287      32      50      32     870

Cuts at:   5530    5562    5802    5834    6103    6135    6949    6981    7004 
   Size:         32     240      32     269      32     814      32      23

Cuts at:   7004    7036    8479    8511    8553    8585    1373 
   Size:         32    1443      32      42      32    2626

  Fragments arranged by size: 

                2626    1443    1287    1076     870     814     412     269
                 240     135      50      42      37      34      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      23
 
HincII GTy'rAC

Cuts at:    669    1657    1938    7182    7428     669 
   Size:        988     281    5244     246    3079

  Fragments arranged by size: 

                5244    3079     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2516    3327    3938    5966     748 
   Size:       1768     811     611    2028    4620

  Fragments arranged by size: 

                4620    2028    1768     811     611
 
HpaI GTT'AAC

Cuts at:   7428    7428 
   Size:       9838
 
KpnI G_GTAC'C

Cuts at:   7163    7163 
   Size:       9838
 
MfeI C'AATT_G

Cuts at:   7437    7437 
   Size:       9838
 
MluI A'CGCG_T

Cuts at:   9714    9714 
   Size:       9838
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3132    3291    6648    6780    7077    8952    9136    1305 
   Size:       1827     159    3357     132     297    1875     184    2007

  Fragments arranged by size: 

                3357    2007    1875    1827     297     184     159     132
            
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2564    3827    5999      10 
   Size:         54    1425     343     732    1263    2172    3849

  Fragments arranged by size: 

                3849    2172    1425    1263     732     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    5330    6809    7796    8155    8314     518 
   Size:        648    4164    1479     987     359     159    2042

  Fragments arranged by size: 

                4164    2042    1479     987     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2041    2197    3298    3583    3796    4183    4393    4588 
   Size:        435     156    1101     285     213     387     210     195

Cuts at:   4588    5024    5741    5831    7062    7254    7931    9079    9324 
   Size:        436     717      90    1231     192     677    1148     245

Cuts at:   9324    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1231    1148    1101     717     677     436     435
                 387     285     245     213     210     195     192     156
                  90
 
NaeI GCC'GGC

Cuts at:   2984    7125    2984 
   Size:       4141    5697
 
NcoI C'CATG_G

Cuts at:    513    1065    6477     513 
   Size:        552    5412    3874

  Fragments arranged by size: 

                5412    3874     552
 
NdeI CA'TA_TG

Cuts at:    387    5356     387 
   Size:       4969    4869
 
NgoMIV G'CCGG_C

Cuts at:   2982    7123    2982 
   Size:       4141    5697
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       9838
 
NotI GC'GGCC_GC

Cuts at:   7285    7285 
   Size:       9838
 
NspI r_CATG'y

Cuts at:   1132    1229    2818    3023    4624    5687    6808    7196    8741 
   Size:         97    1589     205    1601    1063    1121     388    1545

Cuts at:   8741    1132 
   Size:       2229

  Fragments arranged by size: 

                2229    1601    1589    1545    1121    1063     388     205
                  97
 
PasI CC'CwG_GG

Cuts at:   1348    5868    1348 
   Size:       4520    5318
 
PciI A'CATG_T

Cuts at:   4620    8737    4620 
   Size:       4117    5721
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3200    4183    4615    5443    5995    6483    1108 
   Size:       2092     983     432     828     552     488    4463

  Fragments arranged by size: 

                4463    2092     983     828     552     488     432
 
PfoI T'CCnGG_A

Cuts at:   2046    4683    6902    2046 
   Size:       2637    2219    4982

  Fragments arranged by size: 

                4982    2637    2219
 
PmeI GTTT'AAAC

Cuts at:   7142    7142 
   Size:       9838
 
PmlI CAC'GTG

Cuts at:   5197    6778    5197 
   Size:       1581    8257
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   7907    7939    9420    9452    7907 
   Size:         32    1481      32    8293

  Fragments arranged by size: 

                8293    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2947    3301    5314    6682    2947 
   Size:        354    2013    1368    6103

  Fragments arranged by size: 

                6103    2013    1368     354
 
PshAI GACnn'nnGTC

Cuts at:   1613    5641    1613 
   Size:       4028    5810
 
PsiI TTA'TAA

Cuts at:   7408    7408 
   Size:       9838
 
PspOMI G'GGCC_C

Cuts at:   2054    3432    6790    6865    2054 
   Size:       1378    3358      75    5027

  Fragments arranged by size: 

                5027    3358    1378      75
 
PspXI vC'TCGA_Gb

Cuts at:   2171    3524    4541    2171 
   Size:       1353    1017    7468

  Fragments arranged by size: 

                7468    1353    1017
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   6899    6931    6899 
   Size:         32    9806
 
PstI C_TGCA'G

Cuts at:    830    2751    2972    3321    3729    4194    4410    4644    6705 
   Size:       1921     221     349     408     465     216     234    2061

Cuts at:   6705    7190     830 
   Size:        485    3478

  Fragments arranged by size: 

                3478    2061    1921     485     465     408     349     234
                 221     216
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       9838
 
PvuII CAG'CTG

Cuts at:   2041    2197    3796    4183    4393    5741    2041 
   Size:        156    1599     387     210    1348    6138

  Fragments arranged by size: 

                6138    1599    1348     387     210     156
 
SacI G_AGCT'C

Cuts at:   3765    4542    4695    5477    6300    6384    3765 
   Size:        777     153     782     823      84    7219

  Fragments arranged by size: 

                7219     823     782     777     153      84
 
SalI G'TCGA_C

Cuts at:   1936    7180    1936 
   Size:       5244    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2889    3245    4530    5387    2889 
   Size:        356    1285     857    7340

  Fragments arranged by size: 

                7340    1285     857     356
 
SbfI CC_TGCA'GG

Cuts at:   2751    3729    7190    2751 
   Size:        978    3461    5399

  Fragments arranged by size: 

                5399    3461     978
 
ScaI AGT'ACT

Cuts at:   8086    9798    8086 
   Size:       1712    8126
 
SexAI A'CCwGG_T

Cuts at:   6225    6225 
   Size:       9838
 
SfcI C'TryA_G

Cuts at:    826    1045    2747    2968    3317    3725    4190    4406    4640 
   Size:        219    1702     221     349     408     465     216     234

Cuts at:   4640    6409    6701    7186    8321    9002    9193     826 
   Size:       1769     292     485    1135     681     191    1471

  Fragments arranged by size: 

                1769    1702    1471    1135     681     485     465     408
                 349     292     234     221     219     216     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       9838
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       9838
 
SmaI CCC'GGG

Cuts at:   1872    4499    7165    1872 
   Size:       2627    2666    4545

  Fragments arranged by size: 

                4545    2666    2627
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2024    2171    3524    3536    3995    4541 
   Size:        197     926      81     147    1353      12     459     546

Cuts at:   4541    6011    7942    8843    9105     820 
   Size:       1470    1931     901     262    1553

  Fragments arranged by size: 

                1931    1553    1470    1353     926     901     546     459
                 262     197     147      81      12
 
SnaBI TAC'GTA

Cuts at:    493    7629     493 
   Size:       7136    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       9838
 
SphI G_CATG'C

Cuts at:   1132    3023    6808    7196    1132 
   Size:       1891    3785     388    3774

  Fragments arranged by size: 

                3785    3774    1891     388
 
SspI AAT'ATT

Cuts at:      5      52    3150    7762       5 
   Size:         47    3098    4612    2081

  Fragments arranged by size: 

                4612    3098    2081      47
 
StuI AGG'CCT

Cuts at:   1517    3923    4121    5257    7113    1517 
   Size:       2406     198    1136    1856    4242

  Fragments arranged by size: 

                4242    2406    1856    1136     198
 
StyI C'CwwG_G

Cuts at:    513    1065    2013    3684    3927    4722    5691    6477    7277 
   Size:        552     948    1671     243     795     969     786     800

Cuts at:   7277     513 
   Size:       3074

  Fragments arranged by size: 

                3074    1671     969     948     800     795     786     552
                 243
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2465    2795    3413    4547    5080    5429    6440 
   Size:       1255     885     330     618    1134     533     349    1011

Cuts at:   6440    6475    7865    7882    8035    8220    8665     325 
   Size:         35    1390      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1390    1255    1134    1011     885     618     533
                 445     349     330     185     153      35      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2115    6438    8084 
   Size:        274      80      33      51    1443     138    4323    1646

Cuts at:   8084    9796      96 
   Size:       1712     138

  Fragments arranged by size: 

                4323    1712    1646    1443     274     138     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4574    4606    5117    5149    6330    6362    6371    6403    9798 
   Size:         32     511      32    1181      32       9      32    3395

Cuts at:   9798    9830    4574 
   Size:         32    4582

  Fragments arranged by size: 

                4582    3395    1181     511      32      32      32      32
                  32       9
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2618    1316 
   Size:       1302    8536
 
XbaI T'CTAG_A

Cuts at:   5629    7174    5629 
   Size:       1545    8293
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3115    3835    4720    5047    5257    3115 
   Size:        720     885     327     210    7696

  Fragments arranged by size: 

                7696     885     720     327     210
 
XhoI C'TCGA_G

Cuts at:   1943    2171    3524    4541    1943 
   Size:        228    1353    1017    7240

  Fragments arranged by size: 

                7240    1353    1017     228
 
XmaI C'CCGG_G

Cuts at:   1870    4497    7163    1870 
   Size:       2627    2666    4545

  Fragments arranged by size: 

                4545    2666    2627
 
XmnI GAAnn'nnTTC

Cuts at:   4467    6089    7967    4467 
   Size:       1622    1878    6338

  Fragments arranged by size: 

                6338    1878    1622
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    6460    7646     276 
   Size:         53      83     186     936    4926    1186    2468

  Fragments arranged by size: 

                4926    2468    1186     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     AscI    AvrII     BbeI    BfrBI    BsiWI    BsmBI
  BstZ17I   Bsu36I    EcoNI     FseI     KasI     NarI     NruI     NsiI
     PacI    RsrII    SacII    SanDI     SfiI     SfoI     SrfI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com