Restriction map for
pFN21AA1124
(Linear) (Six-base) MAPSORT of: KIAA1124.seq Check: 2959 from: 1 to: 5133
KIAA1124.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4033 4794 5133
Size: 4033 761 339
Fragments arranged by size:
4033 761 339
AatII G_ACGT'C
Cuts at: 0 4457 5133
Size: 4457 676
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 950 1264 3416 4305 4544 5133
Size: 950 314 2152 889 239 589
Fragments arranged by size:
2152 950 889 589 314 239
AflIII A'CryG_T
Cuts at: 0 2615 3191 5133
Size: 2615 576 1942
Fragments arranged by size:
2615 1942 576
AhdI GACnn_n'nnGTC
Cuts at: 0 792 5133
Size: 792 4341
AleI CACnn'nnGTG
Cuts at: 0 3325 5133
Size: 3325 1808
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1396 4822 5133
Size: 1396 3426 311
Fragments arranged by size:
3426 1396 311
AlwNI CAG_nnn'CTG
Cuts at: 0 957 1581 2409 2610 2818 3045 3122 4134
Size: 957 624 828 201 208 227 77 1012
Cuts at: 4134 4157 4641 5133
Size: 23 484 492
Fragments arranged by size:
1012 957 828 624 492 484 227 208
201 77 23
ApaI G_GGCC'C
Cuts at: 0 53 1431 4789 4864 5133
Size: 53 1378 3358 75 269
Fragments arranged by size:
3358 1378 269 75 53
ApaLI G'TGCA_C
Cuts at: 0 589 3551 3907 5133
Size: 589 2962 356 1226
Fragments arranged by size:
2962 1226 589 356
ApoI r'AATT_y
Cuts at: 0 299 499 2777 2803 5133
Size: 299 200 2278 26 2330
Fragments arranged by size:
2330 2278 299 200 26
AvaI C'yCGr_G
Cuts at: 0 166 1424 1519 2492 2536 3235 3803 4587
Size: 166 1258 95 973 44 699 568 784
Cuts at: 4587 5133
Size: 546
Fragments arranged by size:
1258 973 784 699 568 546 166 95
44
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 908 941 5133
Size: 908 33 4192
Fragments arranged by size:
4192 908 33
BanI G'GyrC_C
Cuts at: 0 2020 3208 4021 4528 5133
Size: 2020 1188 813 507 605
Fragments arranged by size:
2020 1188 813 605 507
BanII G_rGCy'C
Cuts at: 0 53 1431 1760 2537 2690 2825 3472 3785
Size: 53 1378 329 777 153 135 647 313
Cuts at: 3785 4295 4379 4789 4864 5071 5133
Size: 510 84 410 75 207 62
Fragments arranged by size:
1378 777 647 510 410 329 313 207
153 135 84 75 62 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 227 512 4662 5133
Size: 227 285 4150 471
Fragments arranged by size:
4150 471 285 227
BbvCI CC'TCA_GC
Cuts at: 0 1432 1913 3661 5133
Size: 1432 481 1748 1472
Fragments arranged by size:
1748 1472 1432 481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 196 230 2470 2504 5133
Size: 196 34 2240 34 2629
Fragments arranged by size:
2629 2240 196 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2650 5133
Size: 2650 2483
BlpI GC'TnA_GC
Cuts at: 0 493 1369 1892 2907 4189 5133
Size: 493 876 523 1015 1282 944
Fragments arranged by size:
1282 1015 944 876 523 493
Bme1580I G_kGCm'C
Cuts at: 0 53 593 715 1431 1767 3213 3226 3555
Size: 53 540 122 716 336 1446 13 329
Cuts at: 3555 3911 4365 4789 4838 4864 5133
Size: 356 454 424 49 26 269
Fragments arranged by size:
1446 716 540 454 424 356 336 329
269 122 53 49 26 13
BmgBI CAC'GTC
Cuts at: 0 96 627 1113 1125 2110 3267 4425 5133
Size: 96 531 486 12 985 1157 1158 708
Fragments arranged by size:
1158 1157 985 708 531 486 96 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 2448 2828 5133
Size: 2448 380 2305
Fragments arranged by size:
2448 2305 380
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4615 5133
Size: 4615 518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 1321 1384 1612 2002 2089 2410 2650
Size: 49 1272 63 228 390 87 321 240
Cuts at: 2650 2668 3084 3619 5131 5133
Size: 18 416 535 1512 2
Fragments arranged by size:
1512 1272 535 416 390 321 240 228
87 63 49 18 2
Bpu10I CC'TnA_GC
Cuts at: 0 73 1432 1873 1913 2689 3661 4144 5133
Size: 73 1359 441 40 776 972 483 989
Fragments arranged by size:
1359 989 972 776 483 441 73 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 1552 1975 3991 5133
Size: 4 1548 423 2016 1142
Fragments arranged by size:
2016 1548 1142 423 4
BsaI GGTCTCn'nnnn_
Cuts at: 0 343 800 856 4715 5133
Size: 343 457 56 3859 418
Fragments arranged by size:
3859 457 418 343 56
BsaAI yAC'GTr
Cuts at: 0 153 588 3192 4341 4773 5133
Size: 153 435 2604 1149 432 360
Fragments arranged by size:
2604 1149 435 432 360 153
BsaBI GATnn'nnATC
Cuts at: 0 1697 3877 5133
Size: 1697 2180 1256
Fragments arranged by size:
2180 1697 1256
BsaHI Gr'CG_yC
Cuts at: 0 1838 2705 4454 5133
Size: 1838 867 1749 679
Fragments arranged by size:
1838 1749 867 679
BsaWI w'CCGG_w
Cuts at: 0 516 3966 5133
Size: 516 3450 1167
Fragments arranged by size:
3450 1167 516
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2541 2571 2623 2653 2916 2946 3112 3142
Size: 2541 30 52 30 263 30 166 30
Cuts at: 3142 4366 4396 5133
Size: 1224 30 737
Fragments arranged by size:
2541 1224 737 263 166 52 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 382 1347 1642 1819 1822 2269 2562 2698
Size: 382 965 295 177 3 447 293 136
Cuts at: 2698 2864 3874 4318 4531 5133
Size: 166 1010 444 213 602
Fragments arranged by size:
1010 965 602 447 444 382 295 293
213 177 166 136 3
BseYI C'CCAG_C
Cuts at: 0 981 3410 5053 5133
Size: 981 2429 1643 80
Fragments arranged by size:
2429 1643 981 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 400 712 1309 1423 1618 3108 3135
Size: 141 259 312 597 114 195 1490 27
Cuts at: 3135 3289 4147 4504 5133
Size: 154 858 357 629
Fragments arranged by size:
1490 858 629 597 357 312 259 195
154 141 114 27
BsiHKAI G_wGCw'C
Cuts at: 0 101 593 1307 1760 1946 2036 2537 2690
Size: 101 492 714 453 186 90 501 153
Cuts at: 2690 3472 3555 3911 4145 4295 4379 4811 5133
Size: 782 83 356 234 150 84 432 322
Fragments arranged by size:
782 714 501 492 453 432 356 322
234 186 153 150 101 90 84 83
Bsp1286I G_dGCh'C
Cuts at: 0 53 101 593 715 1307 1431 1760 1767
Size: 53 48 492 122 592 124 329 7
Cuts at: 1767 1946 2036 2537 2690 2825 3213 3226 3472
Size: 179 90 501 153 135 388 13 246
Cuts at: 3472 3555 3785 3911 4145 4295 4365 4379 4789
Size: 83 230 126 234 150 70 14 410
Cuts at: 4789 4811 4838 4864 5071 5133
Size: 22 27 26 207 62
Fragments arranged by size:
592 501 492 410 388 329 246 234
230 207 179 153 150 135 126 124
122 90 83 70 62 53 48 27
26 22 14 13 7
BspEI T'CCGG_A
Cuts at: 0 516 3966 5133
Size: 516 3450 1167
Fragments arranged by size:
3450 1167 516
BspHI T'CATG_A
Cuts at: 0 878 5133
Size: 878 4255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4033 4319 4794 4826 5133
Size: 4033 286 475 32 307
Fragments arranged by size:
4033 475 307 286 32
BsrBI CCG'CTC
Cuts at: 0 1662 2017 3940 4594 4867 5133
Size: 1662 355 1923 654 273 266
Fragments arranged by size:
1923 1662 654 355 273 266
BsrDI GCAATG_nn'
Cuts at: 0 314 3422 5133
Size: 314 3108 1711
Fragments arranged by size:
3108 1711 314
BsrFI r'CCGG_y
Cuts at: 0 977 3656 5118 5133
Size: 977 2679 1462 15
Fragments arranged by size:
2679 1462 977 15
BsrGI T'GTAC_A
Cuts at: 0 110 4433 5133
Size: 110 4323 700
Fragments arranged by size:
4323 700 110
BssHII G'CGCG_C
Cuts at: 0 3345 4367 5133
Size: 3345 1022 766
Fragments arranged by size:
3345 1022 766
BssSI C'ACGA_G
Cuts at: 0 856 4917 5133
Size: 856 4061 216
Fragments arranged by size:
4061 856 216
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1311 1581 1854 3122 4641 5133
Size: 1311 270 273 1268 1519 492
Fragments arranged by size:
1519 1311 1268 492 273 270
BstEII G'GTnAC_C
Cuts at: 0 2497 3798 5133
Size: 2497 1301 1335
Fragments arranged by size:
2497 1335 1301
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4878 5133
Size: 4878 255
BstYI r'GATC_y
Cuts at: 0 737 2406 2657 3441 4067 4735 5133
Size: 737 1669 251 784 626 668 398
Fragments arranged by size:
1669 784 737 668 626 398 251
BtgI C'CryG_G
Cuts at: 0 701 707 1413 3320 3919 3995 4472 5133
Size: 701 6 706 1907 599 76 477 661
Fragments arranged by size:
1907 706 701 661 599 477 76 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 389 763 2314 5133
Size: 389 374 1551 2819
Fragments arranged by size:
2819 1551 389 374
BtsI GCAGTG_nn'
Cuts at: 0 2867 3120 4135 5133
Size: 2867 253 1015 998
Fragments arranged by size:
2867 1015 998 253
DraIII CAC_nnn'GTG
Cuts at: 0 119 1608 3906 5133
Size: 119 1489 2298 1227
Fragments arranged by size:
2298 1489 1227 119
DrdI GACnn_nn'nnGTC
Cuts at: 0 100 1404 5133
Size: 100 1304 3729
Fragments arranged by size:
3729 1304 100
EaeI y'GGCC_r
Cuts at: 0 5 155 557 704 1820 3891 3992 3998
Size: 5 150 402 147 1116 2071 101 6
Cuts at: 3998 4037 5133
Size: 39 1096
Fragments arranged by size:
2071 1116 1096 402 150 147 101 39
6 5
EarI CTCTTCn'nnn_
Cuts at: 0 243 884 1240 2087 2525 3382 4682 5133
Size: 243 641 356 847 438 857 1300 451
Fragments arranged by size:
1300 857 847 641 451 438 356 243
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 631 2668 5133
Size: 631 2037 2465
Fragments arranged by size:
2465 2037 631
EcoICRI GAG'CTC
Cuts at: 0 1758 2535 2688 3470 4293 4377 5133
Size: 1758 777 153 782 823 84 756
Fragments arranged by size:
1758 823 782 777 756 153 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 950 1264 1321 1384 1612 2002 2089
Size: 49 901 314 57 63 228 390 87
Cuts at: 2089 2410 2650 2668 3084 3416 3619 4305 4544
Size: 321 240 18 416 332 203 686 239
Cuts at: 4544 5131 5133
Size: 587 2
Fragments arranged by size:
901 686 587 416 390 332 321 314
240 239 228 203 87 63 57 49
18 2
EcoO109I rG'GnC_Cy
Cuts at: 0 50 942 1296 1428 1986 3042 3309 4511
Size: 50 892 354 132 558 1056 267 1202
Cuts at: 4511 4677 4861 5133
Size: 166 184 272
Fragments arranged by size:
1202 1056 892 558 354 272 267 184
166 132 50
EcoRV GAT'ATC
Cuts at: 0 4748 5133
Size: 4748 385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 272 529 1912 2097 5133
Size: 272 257 1383 185 3036
Fragments arranged by size:
3036 1383 272 257 185
FspI TGC'GCA
Cuts at: 0 4468 4645 5133
Size: 4468 177 488
Fragments arranged by size:
4468 488 177
FspAI rTGC'GCAy
Cuts at: 0 4468 5133
Size: 4468 665
HaeII r_GCGC'y
Cuts at: 0 71 1549 5133
Size: 71 1478 3584
Fragments arranged by size:
3584 1478 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 643 675 712 744 778 810 1222 1254
Size: 643 32 37 32 34 32 412 32
Cuts at: 1254 2541 2573 2623 2655 3525 3557 3797 3829
Size: 1287 32 50 32 870 32 240 32
Cuts at: 3829 4098 4130 4944 4976 4999 5031 5133
Size: 269 32 814 32 23 32 102
Fragments arranged by size:
1287 870 814 643 412 269 240 102
50 37 34 32 32 32 32 32
32 32 32 32 32 32 23
HindIII A'AGCT_T
Cuts at: 0 511 1322 1933 3961 5133
Size: 511 811 611 2028 1172
Fragments arranged by size:
2028 1172 811 611 511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1127 1286 4643 4775 5072 5133
Size: 1127 159 3357 132 297 61
Fragments arranged by size:
3357 1127 297 159 132 61
MscI TGG'CCA
Cuts at: 0 559 1822 3994 5133
Size: 559 1263 2172 1139
Fragments arranged by size:
2172 1263 1139 559
MslI CAynn'nnrTG
Cuts at: 0 3325 4804 5133
Size: 3325 1479 329
Fragments arranged by size:
3325 1479 329
MspA1I CmG'CkG
Cuts at: 0 36 192 1293 1578 1791 2178 2388 2583
Size: 36 156 1101 285 213 387 210 195
Cuts at: 2583 3019 3736 3826 5057 5133
Size: 436 717 90 1231 76
Fragments arranged by size:
1231 1101 717 436 387 285 213 210
195 156 90 76 36
NaeI GCC'GGC
Cuts at: 0 979 5120 5133
Size: 979 4141 13
Fragments arranged by size:
4141 979 13
NcoI C'CATG_G
Cuts at: 0 4472 5133
Size: 4472 661
NdeI CA'TA_TG
Cuts at: 0 3351 5133
Size: 3351 1782
NgoMIV G'CCGG_C
Cuts at: 0 977 5118 5133
Size: 977 4141 15
Fragments arranged by size:
4141 977 15
NspI r_CATG'y
Cuts at: 0 813 1018 2619 3682 4803 5133
Size: 813 205 1601 1063 1121 330
Fragments arranged by size:
1601 1121 1063 813 330 205
PasI CC'CwG_GG
Cuts at: 0 3863 5133
Size: 3863 1270
PciI A'CATG_T
Cuts at: 0 2615 5133
Size: 2615 2518
PflMI CCAn_nnn'nTGG
Cuts at: 0 1195 2178 2610 3438 3990 4478 5133
Size: 1195 983 432 828 552 488 655
Fragments arranged by size:
1195 983 828 655 552 488 432
PfoI T'CCnGG_A
Cuts at: 0 41 2678 4897 5133
Size: 41 2637 2219 236
Fragments arranged by size:
2637 2219 236 41
PmlI CAC'GTG
Cuts at: 0 3192 4773 5133
Size: 3192 1581 360
Fragments arranged by size:
3192 1581 360
PpuMI rG'GwC_Cy
Cuts at: 0 942 1296 3309 4677 5133
Size: 942 354 2013 1368 456
Fragments arranged by size:
2013 1368 942 456 354
PshAI GACnn'nnGTC
Cuts at: 0 3636 5133
Size: 3636 1497
PspOMI G'GGCC_C
Cuts at: 0 49 1427 4785 4860 5133
Size: 49 1378 3358 75 273
Fragments arranged by size:
3358 1378 273 75 49
PspXI vC'TCGA_Gb
Cuts at: 0 166 1519 2536 5133
Size: 166 1353 1017 2597
Fragments arranged by size:
2597 1353 1017 166
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4894 4926 5133
Size: 4894 32 207
Fragments arranged by size:
4894 207 32
PstI C_TGCA'G
Cuts at: 0 746 967 1316 1724 2189 2405 2639 4700
Size: 746 221 349 408 465 216 234 2061
Cuts at: 4700 5133
Size: 433
Fragments arranged by size:
2061 746 465 433 408 349 234 221
216
PvuII CAG'CTG
Cuts at: 0 36 192 1791 2178 2388 3736 5133
Size: 36 156 1599 387 210 1348 1397
Fragments arranged by size:
1599 1397 1348 387 210 156 36
SacI G_AGCT'C
Cuts at: 0 1760 2537 2690 3472 4295 4379 5133
Size: 1760 777 153 782 823 84 754
Fragments arranged by size:
1760 823 782 777 754 153 84
SapI GCTCTTCn'nnn_
Cuts at: 0 884 1240 2525 3382 5133
Size: 884 356 1285 857 1751
Fragments arranged by size:
1751 1285 884 857 356
SbfI CC_TGCA'GG
Cuts at: 0 746 1724 5133
Size: 746 978 3409
Fragments arranged by size:
3409 978 746
SexAI A'CCwGG_T
Cuts at: 0 4220 5133
Size: 4220 913
SfcI C'TryA_G
Cuts at: 0 742 963 1312 1720 2185 2401 2635 4404
Size: 742 221 349 408 465 216 234 1769
Cuts at: 4404 4696 5133
Size: 292 437
Fragments arranged by size:
1769 742 465 437 408 349 292 234
221 216
SmaI CCC'GGG
Cuts at: 0 2494 5133
Size: 2494 2639
SmlI C'TyrA_G
Cuts at: 0 19 166 1519 1531 1990 2536 4006 5133
Size: 19 147 1353 12 459 546 1470 1127
Fragments arranged by size:
1470 1353 1127 546 459 147 19 12
SphI G_CATG'C
Cuts at: 0 1018 4803 5133
Size: 1018 3785 330
Fragments arranged by size:
3785 1018 330
SspI AAT'ATT
Cuts at: 0 1145 5133
Size: 1145 3988
StuI AGG'CCT
Cuts at: 0 1918 2116 3252 5108 5133
Size: 1918 198 1136 1856 25
Fragments arranged by size:
1918 1856 1136 198 25
StyI C'CwwG_G
Cuts at: 0 8 1679 1922 2717 3686 4472 5133
Size: 8 1671 243 795 969 786 661
Fragments arranged by size:
1671 969 795 786 661 243 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 460 790 1408 2542 3075 3424 4435 4470
Size: 460 330 618 1134 533 349 1011 35
Cuts at: 4470 5133
Size: 663
Fragments arranged by size:
1134 1011 663 618 533 460 349 330
35
TatI w'GTAC_w
Cuts at: 0 110 4433 5133
Size: 110 4323 700
Fragments arranged by size:
4323 700 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2569 2601 3112 3144 4325 4357 4366 4398
Size: 2569 32 511 32 1181 32 9 32
Cuts at: 4398 5133
Size: 735
Fragments arranged by size:
2569 1181 735 511 32 32 32 32
9
Tth111I GACn'n_nGTC
Cuts at: 0 613 5133
Size: 613 4520
XbaI T'CTAG_A
Cuts at: 0 3624 5133
Size: 3624 1509
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1110 1830 2715 3042 3252 5133
Size: 1110 720 885 327 210 1881
Fragments arranged by size:
1881 1110 885 720 327 210
XhoI C'TCGA_G
Cuts at: 0 166 1519 2536 5133
Size: 166 1353 1017 2597
Fragments arranged by size:
2597 1353 1017 166
XmaI C'CCGG_G
Cuts at: 0 2492 5133
Size: 2492 2641
XmnI GAAnn'nnTTC
Cuts at: 0 2462 4084 5133
Size: 2462 1622 1049
Fragments arranged by size:
2462 1622 1049
ZraI GAC'GTC
Cuts at: 0 4455 5133
Size: 4455 678
Enzymes that do cut:
AarI AatII AcuI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BaeI BanI BanII BbsI
BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstXI BstYI BtgI BtgZI BtsI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI
FspI FspAI HaeII Hin4I HindIII MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuII SacI SapI SbfI SexAI SfcI SmaI
SmlI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AloI AscI
AseI AsiSI AvrII BamHI BbeI BciVI BclI BfrBI
BglII BmtI BsiEI BsiWI BsmI BsmBI BstBI BstZ17I
Bsu36I ClaI CspCI DraI EagI EcoNI EcoRI FseI
HincII HpaI KasI KpnI MfeI MluI NarI NheI
NotI NruI NsiI PacI PmeI PpiI PsiI PvuI
RsrII SacII SalI SanDI ScaI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SwaI