(Circular) (Six-base) MAPSORT of: pFN21AA1070.seq Check: 5150 from: 1 to: 7174 pFN21AA1070 7174 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4984 278 Size: 53 83 186 936 3448 2468 Fragments arranged by size: 3448 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3323 3869 4517 1937 Size: 1386 546 648 4594 Fragments arranged by size: 4594 1386 648 546 Acc65I G'GTAC_C Cuts at: 3832 4495 3832 Size: 663 6511 AclI AA'CG_TT Cuts at: 1563 5301 5674 1563 Size: 3738 373 3063 Fragments arranged by size: 3738 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2102 2853 3303 5237 6621 6889 1632 Size: 470 751 450 1934 1384 268 1917 Fragments arranged by size: 1934 1917 1384 751 470 450 268 AflII C'TTAA_G Cuts at: 820 1017 2761 820 Size: 197 1744 5233 Fragments arranged by size: 5233 1744 197 AflIII A'CryG_T Cuts at: 1203 6073 7050 1203 Size: 4870 977 1327 Fragments arranged by size: 4870 1327 977 AgeI A'CCGG_T Cuts at: 6005 6005 Size: 7174 AhdI GACnn_n'nnGTC Cuts at: 4275 5903 4275 Size: 1628 5546 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7174 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7174 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3513 3545 3513 Size: 32 7142 AlwNI CAG_nnn'CTG Cuts at: 4917 6489 4917 Size: 1572 5602 ApaLI G'TGCA_C Cuts at: 5231 6387 5231 Size: 1156 6018 ApoI r'AATT_y Cuts at: 1493 2220 2572 3185 3253 3637 3686 3928 4483 Size: 727 352 613 68 384 49 242 555 Cuts at: 4483 4709 4861 6970 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 727 613 555 384 352 242 226 152 68 49 AseI AT'TA_AT Cuts at: 160 1032 5728 160 Size: 872 4696 1606 Fragments arranged by size: 4696 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7174 AvaI C'yCGr_G Cuts at: 1870 1943 4499 1870 Size: 73 2556 4545 Fragments arranged by size: 4545 2556 73 BamHI G'GATC_C Cuts at: 4504 4871 4504 Size: 367 6807 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2081 3403 3832 4495 5950 Size: 325 217 627 294 1322 429 663 1455 Cuts at: 5950 618 Size: 1842 Fragments arranged by size: 1842 1455 1322 663 627 429 325 294 217 BanII G_rGCy'C Cuts at: 1391 4493 1391 Size: 3102 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2463 928 Size: 971 564 5639 Fragments arranged by size: 5639 971 564 BbvCI CC'TCA_GC Cuts at: 4146 4146 Size: 7174 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2596 2630 2657 2691 5365 5399 1903 Size: 34 659 34 27 34 2674 34 3678 Fragments arranged by size: 3678 2674 659 34 34 34 34 27 BciVI GTATCCnnnnn_n' Cuts at: 5066 6276 5066 Size: 1210 5964 BclI T'GATC_A Cuts at: 1548 1548 Size: 7174 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5785 136 Size: 107 122 71 631 605 4113 1525 Fragments arranged by size: 4113 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7169 7169 Size: 7174 BlpI GC'TnA_GC Cuts at: 4591 4591 Size: 7174 Bme1580I G_kGCm'C Cuts at: 1790 2086 5235 6391 1790 Size: 296 3149 1156 2573 Fragments arranged by size: 3149 2573 1156 296 BmgBI CAC'GTC Cuts at: 1318 3945 1318 Size: 2627 4547 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3340 3551 4905 Size: 416 53 83 399 24 1923 211 1354 Cuts at: 4905 5863 442 Size: 958 1753 Fragments arranged by size: 1923 1753 1354 958 416 399 211 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7174 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3155 3155 Size: 7174 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2280 4428 5834 1239 Size: 1041 2148 1406 2579 Fragments arranged by size: 2579 2148 1406 1041 Bpu10I CC'TnA_GC Cuts at: 4146 4146 Size: 7174 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2677 3660 5299 6164 6462 2677 Size: 983 1639 865 298 3389 Fragments arranged by size: 3389 1639 983 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5837 882 Size: 617 4338 2219 Fragments arranged by size: 4338 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2025 3453 4965 493 Size: 711 821 1428 1512 2702 Fragments arranged by size: 2702 1512 1428 821 711 BsaBI GATnn'nnATC Cuts at: 2618 4224 7168 2618 Size: 1606 2944 2624 Fragments arranged by size: 2944 2624 1606 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4981 5363 5998 275 Size: 53 83 186 936 3448 382 635 1451 Fragments arranged by size: 3448 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5607 6005 6279 6426 6837 5607 Size: 398 274 147 411 5944 Fragments arranged by size: 5944 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3513 3543 3744 3774 1178 Size: 30 165 30 2110 30 201 30 4578 Fragments arranged by size: 4578 2110 201 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4129 4318 1186 Size: 2943 189 4042 Fragments arranged by size: 4042 2943 189 BseYI C'CCAG_C Cuts at: 2789 2910 6377 6873 2789 Size: 121 3467 496 3090 Fragments arranged by size: 3467 3090 496 121 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3633 1163 Size: 573 1897 4704 Fragments arranged by size: 4704 1897 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4624 5385 6413 1540 Size: 275 186 2623 761 1028 2301 Fragments arranged by size: 2623 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5235 5320 6391 5235 Size: 85 1071 6018 Fragments arranged by size: 6018 1071 85 BsmI GAATG_Cn' Cuts at: 3005 4685 4778 3005 Size: 1680 93 5401 Fragments arranged by size: 5401 1680 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3516 3516 Size: 7174 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 4493 5235 5320 6391 1391 Size: 399 296 2407 742 85 1071 2174 Fragments arranged by size: 2407 2174 1071 742 399 296 85 BspEI T'CCGG_A Cuts at: 6837 6837 Size: 7174 BspHI T'CATG_A Cuts at: 4228 5063 6793 4228 Size: 835 1730 4609 Fragments arranged by size: 4609 1730 835 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2817 2938 4529 844 Size: 1973 121 1591 3489 Fragments arranged by size: 3489 1973 1591 121 BsrBI CCG'CTC Cuts at: 1394 1823 5061 1394 Size: 429 3238 3507 Fragments arranged by size: 3507 3238 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2614 2977 3475 5669 5843 65 Size: 1183 1366 363 498 2194 174 1396 Fragments arranged by size: 2194 1396 1366 1183 498 363 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5818 6005 1700 Size: 110 4008 187 2869 Fragments arranged by size: 4008 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7174 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7174 BssSI C'ACGA_G Cuts at: 5234 6246 5234 Size: 1012 6162 BstAPI GCAn_nnn'nTGC Cuts at: 794 2939 794 Size: 2145 5029 BstBI TT'CG_AA Cuts at: 5978 5978 Size: 7174 BstEII G'GTnAC_C Cuts at: 1184 2753 2837 3653 4308 1184 Size: 1569 84 816 655 4050 Fragments arranged by size: 4050 1569 816 655 84 BstXI CCAn_nnnn'nTGG Cuts at: 2313 2313 Size: 7174 BstYI r'GATC_y Cuts at: 1972 2780 3424 4504 4871 5256 5273 6725 6811 Size: 808 644 1080 367 385 17 1452 86 Cuts at: 6811 6823 7169 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1080 808 644 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3324 3870 3324 Size: 546 6628 BtgI C'CryG_G Cuts at: 513 1065 2004 2542 3517 513 Size: 552 939 538 975 4170 Fragments arranged by size: 4170 975 939 552 538 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3225 487 Size: 683 56 1999 4436 Fragments arranged by size: 4436 1999 683 56 BtsI GCAGTG_nn' Cuts at: 2009 2055 3957 4058 4690 5484 5504 2009 Size: 46 1902 101 632 794 20 3679 Fragments arranged by size: 3679 1902 794 632 101 46 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7174 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2323 2358 2944 2979 529 Size: 35 1759 35 586 35 4724 Fragments arranged by size: 4724 1759 586 35 35 35 DraI TTT'AAA Cuts at: 2472 4478 2472 Size: 2006 5168 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6181 809 Size: 507 4865 1802 Fragments arranged by size: 4865 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2027 2125 2952 Size: 54 1425 50 275 18 197 98 827 Cuts at: 2952 4621 5510 7139 8 Size: 1669 889 1629 43 Fragments arranged by size: 1669 1629 1425 889 827 275 197 98 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4621 1537 Size: 275 2809 4090 Fragments arranged by size: 4090 2809 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4179 5104 1353 Size: 2826 925 3423 Fragments arranged by size: 3423 2826 925 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5761 6135 6281 387 Size: 1064 138 4172 374 146 1280 Fragments arranged by size: 4172 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2280 2853 3303 4428 5237 5834 Size: 393 470 178 573 450 1125 809 597 Cuts at: 5834 6621 6889 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1125 809 787 597 573 470 450 393 268 178 EcoO109I rG'GnC_Cy Cuts at: 2228 2516 2228 Size: 288 6886 EcoRI G'AATT_C Cuts at: 4483 4861 4483 Size: 378 6796 EcoRV GAT'ATC Cuts at: 2323 3278 2323 Size: 955 6219 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2411 3770 2411 Size: 1359 5815 FspI TGC'GCA Cuts at: 5680 5680 Size: 7174 HaeII r_GCGC'y Cuts at: 1678 6321 1678 Size: 4643 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3206 3238 3416 3448 4261 Size: 32 135 32 1634 32 178 32 813 Cuts at: 4261 4293 5815 5847 5889 5921 1373 Size: 32 1522 32 42 32 2626 Fragments arranged by size: 2626 1634 1522 813 178 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2629 2806 3034 4518 4764 669 Size: 988 281 691 177 228 1484 246 3079 Fragments arranged by size: 3079 1484 988 691 281 246 228 177 HindIII A'AGCT_T Cuts at: 748 2712 748 Size: 1964 5210 HpaI GTT'AAC Cuts at: 4764 4764 Size: 7174 KpnI G_GTAC'C Cuts at: 3836 4499 3836 Size: 663 6511 MfeI C'AATT_G Cuts at: 4773 4773 Size: 7174 MluI A'CGCG_T Cuts at: 7050 7050 Size: 7174 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2825 3804 4030 6288 6472 1305 Size: 1520 979 226 2258 184 2007 Fragments arranged by size: 2258 2007 1520 979 226 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2029 2127 2954 10 Size: 54 1425 343 197 98 827 4230 Fragments arranged by size: 4230 1425 827 343 197 98 54 MslI CAynn'nnrTG Cuts at: 518 1166 4233 4251 4349 5132 5491 5650 518 Size: 648 3067 18 98 783 359 159 2042 Fragments arranged by size: 3067 2042 783 648 359 159 98 18 MspA1I CmG'CkG Cuts at: 1606 2910 3410 3422 4590 5267 6415 6660 1606 Size: 1304 500 12 1168 677 1148 245 2120 Fragments arranged by size: 2120 1304 1168 1148 677 500 245 12 NcoI C'CATG_G Cuts at: 513 1065 2004 2542 513 Size: 552 939 538 5145 Fragments arranged by size: 5145 939 552 538 NdeI CA'TA_TG Cuts at: 387 2257 2437 387 Size: 1870 180 5124 Fragments arranged by size: 5124 1870 180 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7174 NotI GC'GGCC_GC Cuts at: 4621 4621 Size: 7174 NspI r_CATG'y Cuts at: 1132 1229 2061 4532 6077 1132 Size: 97 832 2471 1545 2229 Fragments arranged by size: 2471 2229 1545 832 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7174 PciI A'CATG_T Cuts at: 6073 6073 Size: 7174 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7174 PmeI GTTT'AAAC Cuts at: 4478 4478 Size: 7174 PmlI CAC'GTG Cuts at: 2025 2025 Size: 7174 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3513 3545 5243 5275 6756 6788 3513 Size: 32 1698 32 1481 32 3899 Fragments arranged by size: 3899 1698 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2516 2516 Size: 7174 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7174 PsiI TTA'TAA Cuts at: 4744 4744 Size: 7174 PstI C_TGCA'G Cuts at: 830 4526 830 Size: 3696 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7174 PvuII CAG'CTG Cuts at: 2910 3410 2910 Size: 500 6674 RsrII CG'GwC_CG Cuts at: 4269 4269 Size: 7174 SalI G'TCGA_C Cuts at: 1936 4516 1936 Size: 2580 4594 SanDI GG'GwC_CC Cuts at: 2516 2516 Size: 7174 SbfI CC_TGCA'GG Cuts at: 4526 4526 Size: 7174 ScaI AGT'ACT Cuts at: 2646 5422 7134 2646 Size: 2776 1712 2686 Fragments arranged by size: 2776 2686 1712 SfcI C'TryA_G Cuts at: 826 1045 3005 3411 3564 4522 5657 6338 6529 Size: 219 1960 406 153 958 1135 681 191 Cuts at: 6529 826 Size: 1471 Fragments arranged by size: 1960 1471 1135 958 681 406 219 191 153 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7174 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7174 SmaI CCC'GGG Cuts at: 1872 4501 1872 Size: 2629 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2656 2761 3675 5278 6179 6441 Size: 197 926 713 105 914 1603 901 262 Cuts at: 6441 820 Size: 1553 Fragments arranged by size: 1603 1553 926 914 901 713 262 197 105 SnaBI TAC'GTA Cuts at: 493 3453 4965 493 Size: 2960 1512 2702 Fragments arranged by size: 2960 2702 1512 SpeI A'CTAG_T Cuts at: 152 2726 152 Size: 2574 4600 SphI G_CATG'C Cuts at: 1132 2061 4532 1132 Size: 929 2471 3774 Fragments arranged by size: 3774 2471 929 SspI AAT'ATT Cuts at: 5 52 5098 5 Size: 47 5046 2081 Fragments arranged by size: 5046 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7174 StyI C'CwwG_G Cuts at: 513 1065 2004 2542 3169 4613 513 Size: 552 939 538 627 1444 3074 Fragments arranged by size: 3074 1444 939 627 552 538 SwaI ATTT'AAAT Cuts at: 2472 2472 Size: 7174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2069 3388 4351 5201 5218 5371 5556 Size: 1255 489 1319 963 850 17 153 185 Cuts at: 5556 6001 325 Size: 445 1498 Fragments arranged by size: 1498 1319 1255 963 850 489 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2644 2660 3538 Size: 274 80 33 51 1443 667 16 878 Cuts at: 3538 4095 5420 7132 96 Size: 557 1325 1712 138 Fragments arranged by size: 1712 1443 1325 878 667 557 274 138 80 51 33 16 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3380 3412 7134 7166 3380 Size: 32 3722 32 3388 Fragments arranged by size: 3722 3388 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7174 XbaI T'CTAG_A Cuts at: 4510 4510 Size: 7174 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2037 2310 2527 3068 2037 Size: 273 217 541 6143 Fragments arranged by size: 6143 541 273 217 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7174 XmaI C'CCGG_G Cuts at: 1870 4499 1870 Size: 2629 4545 XmnI GAAnn'nnTTC Cuts at: 3928 5303 3928 Size: 1375 5799 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4982 276 Size: 53 83 186 936 3448 2468 Fragments arranged by size: 3448 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI SacI SacII SapI SexAI SfiI SfoI SrfI