Restriction map for
pFN21AA1003
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AccIII AcsI
AflII AgeI AhdI AocI ApoI
AscI Asp700I Asp718I AspEI AspI
AvrII BamHI BbrPI BbvCI BclI
BfrI BglII BlnI BlpI Bpu1102I
BseAI BsiEI BspDI BspEI BspHI
BsrDI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MluI
MroI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
RcaI RsrII SalI SfuI SgrAI
SpeI SspI SwaI Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AclI 1407 AACGTT AA^CGTT
Alw44I 1854 GTGCAC G^TGCAC
ApaLI 1854 GTGCAC G^TGCAC
AseI 1951 ATTAAT AT^TAAT
AsnI 1951 ATTAAT AT^TAAT
AviII 539 TGCGCA TGC^GCA
BbeI 1498 GGCGCC GGCGC^C
BbsI 1615 GAAGAC GAAGAC(2/6)
BfaI 1214 CTAG C^TAG
BpuAI 1615 GAAGAC GAAGAC(2/6)
BsiWI 1256 CGTACG C^GTACG
BsmI 1745 GCATTC GAATGC(1/-1)
BspLU11I 1664 ACATGT A^CATGT
BstYI 1736 AGATCC [GA]^GATC[CT]
DraIII 1768 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1842 CCTTGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 539 TGCGCA TGC^GCA
HincII 658 GTCAAC GT[CT]^[GA]AC
HindII 658 GTCAAC GT[CT]^[GA]AC
KasI 1498 GGCGCC G^GCGCC
KspI 1079 CCGCGG CCGC^GG
MaeI 1214 CTAG C^TAG
MfeI 492 CAATTG C^AATTG
MflI 1736 AGATCC [GA]^GATC[CT]
MunI 492 CAATTG C^AATTG
NaeI 1485 GCCGGC GCC^GGC
NarI 1498 GGCGCC GG^CGCC
NcoI 1058 CCATGG C^CATGG
NgoMIV 1485 GCCGGC G^CCGGC
PshBI 1951 ATTAAT AT^TAAT
Psp1406I 1407 AACGTT AA^CGTT
SacI 736 GAGCTC GAGCT^C
SacII 1079 CCGCGG CCGC^GG
SapI 2046 GAAGAGC GCTCTTC(1/4)
SbfI 477 CCTGCAGG CCTGCA^GG
ScaI 1709 AGTACT AGT^ACT
SexAI 2249 ACCTGGT A^CC[AT]GGT
SfiI 2415 GGCCTTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1498 GGCGCC GGC^GCC
SmaI 2515 CCCGGG CCC^GGG
SnaBI 2029 TACGTA TAC^GTA
SphI 2459 GCATGC GCATG^C
Sse8387I 477 CCTGCAGG CCTGCA^GG
Tsp509I 493 AATT ^AATT
XcmI 2339 CCATCTGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1736 AGATCC [GA]^GATC[CT]
XmaCI 2515 CCCGGG C^CCGGG
XmaI 2515 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Aor51HI 1313 AGCGCT AGC^GCT
1757 AGCGCT
ApaI 2518 GGGCCC GGGCC^C
2685 GGGCCC
BanI 1248 GGCACC G^G[CT][GA]CC
1498 GGCGCC
BcgI 1194 CGAGGGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2375 GCAGGAGGATCG
BciVI 1390 GTATCC GTATCC(6/5)
1570 GTATCC
BsaBI 2382 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2652 GATTGCCATC
BsaI 2608 GAGACC GGTCTC(1/5)
585 GGTCTC
BsaWI 106 ACCGGA [AT]^CCGG[AT]
824 ACCGGA
Bsp1407I 1667 TGTACA T^GTACA
2288 TGTACA
BsrBI 973 GAGCGG CCGCTC(-3/-3)
1678 GAGCGG
BsrFI 747 GCCGGT [GA]^CCGG[CT]
1485 GCCGGC
BsrGI 1667 TGTACA T^GTACA
2288 TGTACA
BstXI 112 CCAAACCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1394 CCACCACAGTGG
Cfr10I 747 GCCGGT [GA]^CCGG[CT]
1485 GCCGGC
DrdI 1978 GACCAGTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2248 GACCTGGTGGTC
Eco47III 1313 AGCGCT AGC^GCT
1757 AGCGCT
EcoT14I 1058 CCATGG C^C[AT][AT]GG
1847 CCAAGG
MamI 2382 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2652 GATTGCCATC
MseI 1651 TTAA T^TAA
1952 TTAA
NspI 1664 ACATGT [GA]CATG^[CT]
2459 GCATGC
PflMI 576 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2664 CCAGAGTGTGG
PpuMI 14 GGGACCT [GA]G^G[AT]CC[CT]
1445 AGGACCT
SspBI 1667 TGTACA T^GTACA
2288 TGTACA
StuI 426 AGGCCT AGG^CCT
2414 AGGCCT
StyI 1058 CCATGG C^C[AT][AT]GG
1847 CCAAGG
Tru9I 1651 TTAA T^TAA
1952 TTAA
Van91I 576 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2664 CCAGAGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII BalI BanII BbvI BcnI
BglI BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BssSI
BstEII BstF5I BstNI BstPI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoO65I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PaeR7I
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XhoI