Restriction map for
pFN21AA0968
(Linear) (Six-base) MAPSORT of: KIAA0968.seq Check: 8593 from: 1 to: 1434
KIAA0968.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 22 1434
Size: 22 1412
Acc65I G'GTAC_C
Cuts at: 0 626 1434
Size: 626 808
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 427 889 916 1434
Size: 220 207 462 27 518
Fragments arranged by size:
518 462 220 207 27
AflII C'TTAA_G
Cuts at: 0 796 1434
Size: 796 638
AleI CACnn'nnGTG
Cuts at: 0 1030 1434
Size: 1030 404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1243 1434
Size: 1243 191
AlwNI CAG_nnn'CTG
Cuts at: 0 168 341 357 1311 1434
Size: 168 173 16 954 123
Fragments arranged by size:
954 173 168 123 16
ApaLI G'TGCA_C
Cuts at: 0 394 1228 1434
Size: 394 834 206
Fragments arranged by size:
834 394 206
AvaI C'yCGr_G
Cuts at: 0 308 1434
Size: 308 1126
BanI G'GyrC_C
Cuts at: 0 445 626 682 1364 1413 1434
Size: 445 181 56 682 49 21
Fragments arranged by size:
682 445 181 56 49 21
BbeI G_GCGC'C
Cuts at: 0 686 1417 1434
Size: 686 731 17
Fragments arranged by size:
731 686 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 1028 1434
Size: 1028 406
BciVI GTATCCnnnnn_n'
Cuts at: 0 355 1434
Size: 355 1079
BclI T'GATC_A
Cuts at: 0 740 1434
Size: 740 694
BglI GCCn_nnn'nGGC
Cuts at: 0 1376 1434
Size: 1376 58
BglII A'GATC_T
Cuts at: 0 1405 1434
Size: 1405 29
Bme1580I G_kGCm'C
Cuts at: 0 398 1232 1434
Size: 398 834 202
Fragments arranged by size:
834 398 202
BmrI ACTGGGnnnn_n'
Cuts at: 0 1335 1434
Size: 1335 99
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 925 1434
Size: 925 509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 379 1434
Size: 190 189 1055
Fragments arranged by size:
1055 190 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 1434
Size: 424 1010
BsaI GGTCTCn'nnnn_
Cuts at: 0 149 847 1345 1434
Size: 149 698 498 89
Fragments arranged by size:
698 498 149 89
BsaHI Gr'CG_yC
Cuts at: 0 683 1414 1434
Size: 683 731 20
Fragments arranged by size:
731 683 20
BsaWI w'CCGG_w
Cuts at: 0 940 1434
Size: 940 494
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 1363 1434
Size: 256 1107 71
Fragments arranged by size:
1107 256 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 1434
Size: 355 1079
BsiHKAI G_wGCw'C
Cuts at: 0 398 1000 1232 1434
Size: 398 602 232 202
Fragments arranged by size:
602 398 232 202
BsiWI C'GTAC_G
Cuts at: 0 566 1434
Size: 566 868
Bsp1286I G_dGCh'C
Cuts at: 0 398 1000 1232 1434
Size: 398 602 232 202
Fragments arranged by size:
602 398 232 202
BspEI T'CCGG_A
Cuts at: 0 940 1434
Size: 940 494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 22 1434
Size: 22 1412
BsrBI CCG'CTC
Cuts at: 0 821 1434
Size: 821 613
BsrDI GCAATG_nn'
Cuts at: 0 1092 1434
Size: 1092 342
BsrFI r'CCGG_y
Cuts at: 0 679 1434
Size: 679 755
BsrGI T'GTAC_A
Cuts at: 0 608 1434
Size: 608 826
BstBI TT'CG_AA
Cuts at: 0 1139 1434
Size: 1139 295
BstXI CCAn_nnnn'nTGG
Cuts at: 0 486 921 1408 1434
Size: 486 435 487 26
Fragments arranged by size:
487 486 435 26
BstYI r'GATC_y
Cuts at: 0 353 735 809 1405 1434
Size: 353 382 74 596 29
Fragments arranged by size:
596 382 353 74 29
Bsu36I CC'TnA_GG
Cuts at: 0 1145 1434
Size: 1145 289
BtgI C'CryG_G
Cuts at: 0 827 857 1434
Size: 827 30 577
Fragments arranged by size:
827 577 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 683 986 1269 1434
Size: 683 303 283 165
Fragments arranged by size:
683 303 283 165
BtsI GCAGTG_nn'
Cuts at: 0 372 458 1434
Size: 372 86 976
Fragments arranged by size:
976 372 86
ClaI AT'CG_AT
Cuts at: 0 1185 1434
Size: 1185 249
EaeI y'GGCC_r
Cuts at: 0 2 105 476 923 1434
Size: 2 103 371 447 511
Fragments arranged by size:
511 447 371 103 2
EarI CTCTTCn'nnn_
Cuts at: 0 26 49 943 961 1434
Size: 26 23 894 18 473
Fragments arranged by size:
894 473 26 23 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 343 1434
Size: 343 1091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 220 379 427 889 916 1434
Size: 190 30 159 48 462 27 518
Fragments arranged by size:
518 462 190 159 48 30 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1165 1434
Size: 1165 269
EcoO109I rG'GnC_Cy
Cuts at: 0 402 561 1149 1170 1434
Size: 402 159 588 21 264
Fragments arranged by size:
588 402 264 159 21
EcoRV GAT'ATC
Cuts at: 0 300 535 1434
Size: 300 235 899
Fragments arranged by size:
899 300 235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 84 1434
Size: 84 1350
HaeII r_GCGC'y
Cuts at: 0 686 1417 1434
Size: 686 731 17
Fragments arranged by size:
731 686 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 682 714 1084 1116 1434
Size: 220 32 430 32 370 32 318
Fragments arranged by size:
430 370 318 220 32 32 32
KasI G'GCGC_C
Cuts at: 0 682 1413 1434
Size: 682 731 21
Fragments arranged by size:
731 682 21
KpnI G_GTAC'C
Cuts at: 0 630 1434
Size: 630 804
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 1325 1434
Size: 243 1082 109
Fragments arranged by size:
1082 243 109
MscI TGG'CCA
Cuts at: 0 4 107 478 925 1434
Size: 4 103 371 447 509
Fragments arranged by size:
509 447 371 103 4
MslI CAynn'nnrTG
Cuts at: 0 484 916 1030 1111 1434
Size: 484 432 114 81 323
Fragments arranged by size:
484 432 323 114 81
MspA1I CmG'CkG
Cuts at: 0 784 1068 1434
Size: 784 284 366
Fragments arranged by size:
784 366 284
NaeI GCC'GGC
Cuts at: 0 681 1434
Size: 681 753
NarI GG'CG_CC
Cuts at: 0 683 1414 1434
Size: 683 731 20
Fragments arranged by size:
731 683 20
NgoMIV G'CCGG_C
Cuts at: 0 679 1434
Size: 679 755
NspI r_CATG'y
Cuts at: 0 843 1434
Size: 843 591
PasI CC'CwG_GG
Cuts at: 0 1152 1434
Size: 1152 282
PflMI CCAn_nnn'nTGG
Cuts at: 0 384 1263 1434
Size: 384 879 171
Fragments arranged by size:
879 384 171
PfoI T'CCnGG_A
Cuts at: 0 356 528 1210 1434
Size: 356 172 682 224
Fragments arranged by size:
682 356 224 172
PpuMI rG'GwC_Cy
Cuts at: 0 402 561 1434
Size: 402 159 873
Fragments arranged by size:
873 402 159
PshAI GACnn'nnGTC
Cuts at: 0 1357 1434
Size: 1357 77
PsiI TTA'TAA
Cuts at: 0 1051 1434
Size: 1051 383
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 619 651 1434
Size: 619 32 783
Fragments arranged by size:
783 619 32
PvuII CAG'CTG
Cuts at: 0 784 1068 1434
Size: 784 284 366
Fragments arranged by size:
784 366 284
SapI GCTCTTCn'nnn_
Cuts at: 0 49 961 1434
Size: 49 912 473
Fragments arranged by size:
912 473 49
SexAI A'CCwGG_T
Cuts at: 0 269 1160 1434
Size: 269 891 274
Fragments arranged by size:
891 274 269
SfoI GGC'GCC
Cuts at: 0 684 1415 1434
Size: 684 731 19
Fragments arranged by size:
731 684 19
SmaI CCC'GGG
Cuts at: 0 310 1434
Size: 310 1124
SmlI C'TyrA_G
Cuts at: 0 439 796 1434
Size: 439 357 638
Fragments arranged by size:
638 439 357
SphI G_CATG'C
Cuts at: 0 843 1434
Size: 843 591
StyI C'CwwG_G
Cuts at: 0 731 1434
Size: 731 703
TatI w'GTAC_w
Cuts at: 0 608 1434
Size: 608 826
Tth111I GACn'n_nGTC
Cuts at: 0 271 715 1434
Size: 271 444 719
Fragments arranged by size:
719 444 271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 704 1434
Size: 704 730
XmaI C'CCGG_G
Cuts at: 0 308 1434
Size: 308 1126
XmnI GAAnn'nnTTC
Cuts at: 0 874 1434
Size: 874 560
Enzymes that do cut:
AarI Acc65I AcuI AflII AleI AlfI AlwNI ApaLI
AvaI BanI BbeI BbsI BciVI BclI BglI BglII
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaHI BsaWI
BseRI BsgI BsiHKAI BsiWI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PflMI PfoI PpuMI PshAI PsiI PsrI PvuII SapI
SexAI SfoI SmaI SmlI SphI StyI TatI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflIII AgeI AhdI AloI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BanII BbvCI BcgI BfrBI BlpI BmgBI BmtI Bpu10I
BsaAI BsaBI BsaXI BseYI BsiEI BsmI BsmBI BspHI
BssHII BssSI BstAPI BstEII BstZ17I CspCI DraI DraIII
DrdI EagI EcoICRI EcoRI FseI FspI FspAI HincII
HindIII HpaI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PspOMI
PspXI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfcI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI SwaI TaqII TstI XbaI XhoI
ZraI