Restriction map for
pFN21AA0953
(Circular) (Six-base) MAPSORT of: pFN21AA0953.seq Check: 9015 from: 1 to: 6889
pFN21AA0953 6889 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4096 4096
Size: 6889
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4699 278
Size: 53 83 186 936 3163 2468
Fragments arranged by size:
3163 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3762 4232 1937
Size: 1825 470 4594
Fragments arranged by size:
4594 1825 470
Acc65I G'GTAC_C
Cuts at: 4210 4210
Size: 6889
AclI AA'CG_TT
Cuts at: 1563 5016 5389 1563
Size: 3453 373 3063
Fragments arranged by size:
3453 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3533 4952 6336 6604 1632
Size: 1901 1419 1384 268 1917
Fragments arranged by size:
1917 1901 1419 1384 268
AfeI AGC'GCT
Cuts at: 2917 3185 2917
Size: 268 6621
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6692
AflIII A'CryG_T
Cuts at: 1203 2220 5788 6765 1203
Size: 1017 3568 977 1327
Fragments arranged by size:
3568 1327 1017 977
AgeI A'CCGG_T
Cuts at: 5720 5720
Size: 6889
AhdI GACnn_n'nnGTC
Cuts at: 2401 5618 2401
Size: 3217 3672
AleI CACnn'nnGTG
Cuts at: 1166 3827 1166
Size: 2661 4228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6889
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3107 3139 3107
Size: 32 6857
AlwNI CAG_nnn'CTG
Cuts at: 2887 3833 4632 6204 2887
Size: 946 799 1572 3572
Fragments arranged by size:
3572 1572 946 799
ApaI G_GGCC'C
Cuts at: 2994 2994
Size: 6889
ApaLI G'TGCA_C
Cuts at: 4946 6102 4946
Size: 1156 5733
ApoI r'AATT_y
Cuts at: 1493 2490 3411 4198 4424 4576 6685 1493
Size: 997 921 787 226 152 2109 1697
Fragments arranged by size:
2109 1697 997 921 787 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5443 160
Size: 872 4411 1606
Fragments arranged by size:
4411 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6889
AvaI C'yCGr_G
Cuts at: 1870 1943 2084 2458 3899 4214 1870
Size: 73 141 374 1441 315 4545
Fragments arranged by size:
4545 1441 374 315 141 73
BamHI G'GATC_C
Cuts at: 2080 4219 4586 2080
Size: 2139 367 4383
Fragments arranged by size:
4383 2139 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2165 2357 3089 4210
Size: 325 217 627 243 135 192 732 1121
Cuts at: 4210 5665 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1121 732 627 325 243 217
192 135
BanII G_rGCy'C
Cuts at: 1391 2687 2994 3155 3963 4208 1391
Size: 1296 307 161 808 245 4072
Fragments arranged by size:
4072 1296 808 307 245 161
BbeI G_GCGC'C
Cuts at: 2169 2169
Size: 6889
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2108 2540 2702 3544 928
Size: 971 209 432 162 842 4273
Fragments arranged by size:
4273 971 842 432 209 162
BbvCI CC'TCA_GC
Cuts at: 3023 4044 3023
Size: 1021 5868
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5080 5114 1903
Size: 34 3143 34 3678
Fragments arranged by size:
3678 3143 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3328 4781 5991 3328
Size: 1453 1210 4226
Fragments arranged by size:
4226 1453 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6889
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5500 136
Size: 107 122 71 631 605 3828 1525
Fragments arranged by size:
3828 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6884 6884
Size: 6889
BlpI GC'TnA_GC
Cuts at: 3038 4306 3038
Size: 1268 5621
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2994 3778 4950 6106 1790
Size: 245 959 784 1172 1156 2573
Fragments arranged by size:
2573 1172 1156 959 784 245
BmgBI CAC'GTC
Cuts at: 1318 2197 3730 1318
Size: 879 1533 4477
Fragments arranged by size:
4477 1533 879
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4620 5578 442
Size: 416 53 83 399 24 3203 958 1753
Fragments arranged by size:
3203 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6889
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2661 4114 2661
Size: 1453 5436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 2345 3023 5549 1239
Size: 888 218 678 2526 2579
Fragments arranged by size:
2579 2526 888 678 218
Bpu10I CC'TnA_GC
Cuts at: 3023 3596 4044 3023
Size: 573 448 5868
Fragments arranged by size:
5868 573 448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2288 5014 5879 6177 2288
Size: 2726 865 298 3000
Fragments arranged by size:
3000 2726 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2649 3860 4085 5552 882
Size: 617 1150 1211 225 1467 2219
Fragments arranged by size:
2219 1467 1211 1150 617 225
BsaAI yAC'GTr
Cuts at: 493 1204 2221 4680 493
Size: 711 1017 2459 2702
Fragments arranged by size:
2702 2459 1017 711
BsaBI GATnn'nnATC
Cuts at: 3437 4059 6883 3437
Size: 622 2824 3443
Fragments arranged by size:
3443 2824 622
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2166 2901 4696 5078
Size: 53 83 186 936 633 735 1795 382
Cuts at: 5078 5713 275
Size: 635 1451
Fragments arranged by size:
1795 1451 936 735 635 633 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2412 4132 5322 5720 5994 6141 6552 2412
Size: 1720 1190 398 274 147 411 2749
Fragments arranged by size:
2749 1720 1190 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3107 3137 3170 3200 1178
Size: 30 165 30 1704 30 33 30 4867
Fragments arranged by size:
4867 1704 165 33 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2405 2642 3128 3698 3761 3953 4090 4093
Size: 1219 237 486 570 63 192 137 3
Cuts at: 4093 4157 1186
Size: 64 3918
Fragments arranged by size:
3918 1219 570 486 237 192 137 64
63 3
BseYI C'CCAG_C
Cuts at: 2722 3570 6092 6588 2722
Size: 848 2522 496 3023
Fragments arranged by size:
3023 2522 848 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3218 3641 1163
Size: 573 1482 423 4411
Fragments arranged by size:
4411 1482 573 423
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4339 5100 6128 1540
Size: 275 186 2338 761 1028 2301
Fragments arranged by size:
2338 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3111 3536 3963 4950 5035 6106 3111
Size: 425 427 987 85 1071 3894
Fragments arranged by size:
3894 1071 987 427 425 85
BsmI GAATG_Cn'
Cuts at: 4400 4493 4400
Size: 93 6796
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2687 2994 3111 3155 3536 3778
Size: 399 245 652 307 117 44 381 242
Cuts at: 3778 3963 4208 4950 5035 6106 1391
Size: 185 245 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 652 399 381 307 245
245 242 185 117 85 44
BspEI T'CCGG_A
Cuts at: 4132 6552 4132
Size: 2420 4469
BspHI T'CATG_A
Cuts at: 3207 3555 4778 6508 3207
Size: 348 1223 1730 3588
Fragments arranged by size:
3588 1730 1223 348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2352 3304 4096 4244 844
Size: 1508 952 792 148 3489
Fragments arranged by size:
3489 1508 952 792 148
BsrBI CCG'CTC
Cuts at: 1394 1823 4776 1394
Size: 429 2953 3507
Fragments arranged by size:
3507 2953 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2226 5384 5558 65
Size: 1183 978 3158 174 1396
Fragments arranged by size:
3158 1396 1183 978 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2640 2978 4020 5533 5720 1700
Size: 110 830 338 1042 1513 187 2869
Fragments arranged by size:
2869 1513 1042 830 338 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6889
BssHII G'CGCG_C
Cuts at: 1925 2929 1925
Size: 1004 5885
BssSI C'ACGA_G
Cuts at: 3110 4949 5961 3110
Size: 1839 1012 4038
Fragments arranged by size:
4038 1839 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6889
BstBI TT'CG_AA
Cuts at: 2494 5693 2494
Size: 3199 3690
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6889
BstXI CCAn_nnnn'nTGG
Cuts at: 2213 2323 3001 4168 2213
Size: 110 678 1167 4934
Fragments arranged by size:
4934 1167 678 110
BstYI r'GATC_y
Cuts at: 1972 2080 2349 4219 4586 4971 4988 6440 6526
Size: 108 269 1870 367 385 17 1452 86
Cuts at: 6526 6538 6884 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1870 1452 385 367 346 269 108
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2076 2076
Size: 6889
BtgI C'CryG_G
Cuts at: 513 1065 2688 2961 3147 3221 513
Size: 552 1623 273 186 74 4181
Fragments arranged by size:
4181 1623 552 273 186 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2953 487
Size: 683 56 1727 4423
Fragments arranged by size:
4423 1727 683 56
BtsI GCAGTG_nn'
Cuts at: 2266 2600 4009 4405 5199 5219 2266
Size: 334 1409 396 794 20 3936
Fragments arranged by size:
3936 1409 794 396 334 20
ClaI AT'CG_AT
Cuts at: 1554 2209 1554
Size: 655 6234
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6854
DraI TTT'AAA
Cuts at: 2830 4193 2830
Size: 1363 5526
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3077 3506 5896 809
Size: 507 1761 429 2390 1802
Fragments arranged by size:
2390 1802 1761 507 429
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2313 3224 4336
Size: 54 1425 50 275 18 483 911 1112
Cuts at: 4336 5225 6854 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1112 911 889 483 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4336 1537
Size: 275 2524 4090
Fragments arranged by size:
4090 2524 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2291 3206 3971 4819 1353
Size: 938 915 765 848 3423
Fragments arranged by size:
3423 938 915 848 765
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3959 5476 5850 5996 387
Size: 1064 138 2370 1517 374 146 1280
Fragments arranged by size:
2370 1517 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3961 3961
Size: 6889
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2345 3023 3533 4952 5549 6336
Size: 393 495 218 678 510 1419 597 787
Cuts at: 6336 6604 1239
Size: 268 1524
Fragments arranged by size:
1524 1419 787 678 597 510 495 393
268 218
EcoNI CCTnn'n_nnAGG
Cuts at: 3236 3311 3398 3236
Size: 75 87 6727
Fragments arranged by size:
6727 87 75
EcoO109I rG'GnC_Cy
Cuts at: 2569 2990 3217 2569
Size: 421 227 6241
Fragments arranged by size:
6241 421 227
EcoRI G'AATT_C
Cuts at: 4198 4576 4198
Size: 378 6511
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 6889
FspI TGC'GCA
Cuts at: 5395 5395
Size: 6889
HaeII r_GCGC'y
Cuts at: 1678 2169 2919 3187 3641 6036 1678
Size: 491 750 268 454 2395 2531
Fragments arranged by size:
2531 2395 750 491 454 268
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2387 2419 2627 2659 3518
Size: 32 135 32 815 32 208 32 859
Cuts at: 3518 3550 4052 4080 4084 4103 4112 4114 4135
Size: 32 502 28 4 19 9 2 21
Cuts at: 4135 4146 5530 5562 5604 5636 1373
Size: 11 1384 32 42 32 2626
Fragments arranged by size:
2626 1384 859 815 502 208 135 42
32 32 32 32 32 32 32 28
21 19 11 9 4 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2062 4233 4479 669
Size: 988 281 124 2171 246 3079
Fragments arranged by size:
3079 2171 988 281 246 124
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6889
HpaI GTT'AAC
Cuts at: 4479 4479
Size: 6889
KasI G'GCGC_C
Cuts at: 2165 2165
Size: 6889
KpnI G_GTAC'C
Cuts at: 4214 4214
Size: 6889
MfeI C'AATT_G
Cuts at: 4488 4488
Size: 6889
MluI A'CGCG_T
Cuts at: 6765 6765
Size: 6889
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2934 6003 6187 1305
Size: 1629 3069 184 2007
Fragments arranged by size:
3069 2007 1629 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2315 10
Size: 54 1425 343 483 4584
Fragments arranged by size:
4584 1425 483 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2211 2231 3827 3852 4847 5206 5365
Size: 648 1045 20 1596 25 995 359 159
Cuts at: 5365 518
Size: 2042
Fragments arranged by size:
2042 1596 1045 995 648 359 159 25
20
MspA1I CmG'CkG
Cuts at: 1606 2251 2690 2963 3031 3223 3771 4048 4305
Size: 645 439 273 68 192 548 277 257
Cuts at: 4305 4982 6130 6375 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 645 548 439 277 273
257 245 192 68
NarI GG'CG_CC
Cuts at: 2166 2166
Size: 6889
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6337
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6889
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6889
NotI GC'GGCC_GC
Cuts at: 4336 4336
Size: 6889
NspI r_CATG'y
Cuts at: 1132 1229 2624 3124 3895 4247 5792 1132
Size: 97 1395 500 771 352 1545 2229
Fragments arranged by size:
2229 1545 1395 771 500 352 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6889
PciI A'CATG_T
Cuts at: 5788 5788
Size: 6889
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2565 3244 4186 1108
Size: 1457 679 942 3811
Fragments arranged by size:
3811 1457 942 679
PfoI T'CCnGG_A
Cuts at: 3126 3369 3126
Size: 243 6646
PmeI GTTT'AAAC
Cuts at: 4193 4193
Size: 6889
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3107 3139 4958 4990 6471 6503 3107
Size: 32 1819 32 1481 32 3493
Fragments arranged by size:
3493 1819 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2569 3217 2569
Size: 648 6241
PshAI GACnn'nnGTC
Cuts at: 1613 2199 1613
Size: 586 6303
PsiI TTA'TAA
Cuts at: 4459 4459
Size: 6889
PspOMI G'GGCC_C
Cuts at: 2990 2990
Size: 6889
PspXI vC'TCGA_Gb
Cuts at: 2458 2458
Size: 6889
PstI C_TGCA'G
Cuts at: 830 2349 2556 3315 4107 4241 830
Size: 1519 207 759 792 134 3478
Fragments arranged by size:
3478 1519 792 759 207 134
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6889
PvuII CAG'CTG
Cuts at: 2251 3031 4048 2251
Size: 780 1017 5092
Fragments arranged by size:
5092 1017 780
RsrII CG'GwC_CG
Cuts at: 4018 4018
Size: 6889
SacI G_AGCT'C
Cuts at: 3963 3963
Size: 6889
SacII CC_GC'GG
Cuts at: 2691 2964 3224 2691
Size: 273 260 6356
Fragments arranged by size:
6356 273 260
SalI G'TCGA_C
Cuts at: 1936 4231 1936
Size: 2295 4594
SanDI GG'GwC_CC
Cuts at: 2569 2569
Size: 6889
SbfI CC_TGCA'GG
Cuts at: 3315 4107 4241 3315
Size: 792 134 5963
Fragments arranged by size:
5963 792 134
ScaI AGT'ACT
Cuts at: 3000 5137 6849 3000
Size: 2137 1712 3040
Fragments arranged by size:
3040 2137 1712
SexAI A'CCwGG_T
Cuts at: 2868 2868
Size: 6889
SfcI C'TryA_G
Cuts at: 826 1045 2345 2552 2615 3311 3580 4103 4237
Size: 219 1300 207 63 696 269 523 134
Cuts at: 4237 5372 6053 6244 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1300 1135 696 681 523 269 219
207 191 134 63
SfoI GGC'GCC
Cuts at: 2167 2167
Size: 6889
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6889
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6889
SmaI CCC'GGG
Cuts at: 1872 4216 1872
Size: 2344 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2303 2458 4993 5894 6156 820
Size: 197 926 360 155 2535 901 262 1553
Fragments arranged by size:
2535 1553 926 901 360 262 197 155
SnaBI TAC'GTA
Cuts at: 493 4680 493
Size: 4187 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6889
SphI G_CATG'C
Cuts at: 1132 4247 1132
Size: 3115 3774
SspI AAT'ATT
Cuts at: 5 52 4813 5
Size: 47 4761 2081
Fragments arranged by size:
4761 2081 47
StuI AGG'CCT
Cuts at: 1517 2514 1517
Size: 997 5892
StyI C'CwwG_G
Cuts at: 513 1065 2805 4328 513
Size: 552 1740 1523 3074
Fragments arranged by size:
3074 1740 1523 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4916 4933 5086 5271 5716 325
Size: 1255 3336 17 153 185 445 1498
Fragments arranged by size:
3336 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2841 2998 5135
Size: 274 80 33 51 1443 864 157 2137
Cuts at: 5135 6847 96
Size: 1712 138
Fragments arranged by size:
2137 1712 1443 864 274 157 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6849 6881 6849
Size: 32 6857
Tth111I GACn'n_nGTC
Cuts at: 1316 3548 1316
Size: 2232 4657
XbaI T'CTAG_A
Cuts at: 3427 4225 3427
Size: 798 6091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2983 3394 2983
Size: 411 6478
XhoI C'TCGA_G
Cuts at: 1943 2458 1943
Size: 515 6374
XmaI C'CCGG_G
Cuts at: 1870 4214 1870
Size: 2344 4545
XmnI GAAnn'nnTTC
Cuts at: 5018 5018
Size: 6889
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4697 276
Size: 53 83 186 936 3163 2468
Fragments arranged by size:
3163 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PsrI SapI SfiI SrfI SwaI