Restriction map for
pFN21AA0946
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII AccII AccIII AclI AflII
Alw44I AocI ApaI ApaLI AscI
Asp700I AspHI AvaI AviII AvrII
BalI BanII BbeI BbsI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
BstAPI BstEII BstPI BstUI Bsu36I
CelII ClaI CpoI DrdI EaeI
EagI EclXI Eco52I Eco81I EcoNI
EcoO65I FseI FspI HindIII KasI
KspI MamI MluI MluNI MroI
MscI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NspI PacI
PaeR7I PmeI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 68 enzymes) #
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Acc65I 1883 GGTACC G^GTACC
AccI 1848 GTATAC GT^[AC][GT]AC
AcyI 573 GACGCC G[GA]^CG[CT]C
AflIII 1576 ACGTGT A^C[GA][CT]GT
AgeI 1338 ACCGGT A^CCGGT
Aor51HI 780 AGCGCT AGC^GCT
AseI 453 ATTAAT AT^TAAT
AsnI 453 ATTAAT AT^TAAT
Asp718I 1883 GGTACC G^GTACC
AspI 178 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 612 GGATCC G^GATCC
BanI 1883 GGTACC G^G[CT][GA]CC
BbrPI 1575 CACGTG CAC^GTG
BcnI 42 CCCGG CC^[GC]GG
BmyI 73 GTGCCC G[AGT]GC[ACT]^C
BpmI 740 CTGGAG CTGGAG(16/14)
BsaAI 1575 CACGTG [CT]AC^GT[GA]
BsaHI 573 GACGCC G[GA]^CG[CT]C
BsaI 1525 GAGACC GGTCTC(1/5)
BsmI 1701 GAATGC GAATGC(1/-1)
Bsp1286I 73 GTGCCC G[AGT]GC[ACT]^C
BspMI 235 GCAGGT ACCTGC(4/8)
BsrFI 1338 ACCGGT [GA]^CCGG[CT]
Bst1107I 1848 GTATAC GTA^TAC
BstBI 330 TTCGAA TT^CGAA
BstZ17I 1848 GTATAC GTA^TAC
CfoI 781 GCGC GCG^C
Cfr10I 1338 ACCGGT [GA]^CCGG[CT]
DraI 1723 TTTAAA TTT^AAA
DraII 362 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 602 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1249 GAAGAG CTCTTC(1/4)
Eco47III 780 AGCGCT AGC^GCT
EcoO109I 362 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 369 GAATTC G^AATTC
EcoRV 1665 GATATC GAT^ATC
EcoT22I 1060 ATGCAT ATGCA^T
HaeII 780 AGCGCT [GA]GCGC^[CT]
HgaI 573 GACGC GACGC(5/10)
HhaI 781 GCGC GCG^C
Hin1I 573 GACGCC G[GA]^CG[CT]C
HinP1I 781 GCGC G^CGC
HincII 649 GTTAAC GT[CT]^[GA]AC
HindII 649 GTTAAC GT[CT]^[GA]AC
HpaI 649 GTTAAC GTT^AAC
KpnI 1883 GGTACC GGTAC^C
Ksp632I 1249 GAAGAG CTCTTC(1/4)
MfeI 512 CAATTG C^AATTG
MunI 512 CAATTG C^AATTG
NciI 42 CCCGG CC^[GC]GG
NheI 699 GCTAGC G^CTAGC
NsiI 1060 ATGCAT ATGCA^T
NspV 330 TTCGAA TT^CGAA
PflFI 178 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1097 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1338 ACCGGT A^CCGGT
PleI 1514 GACTC GAGTC(4/5)
PmaCI 1575 CACGTG CAC^GTG
PmlI 1575 CACGTG CAC^GTG
PpuMI 362 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 453 ATTAAT AT^TAAT
PstI 1314 CTGCAG CTGCA^G
PvuII 1888 CAGCTG CAG^CTG
SapI 1249 GAAGAGC GCTCTTC(1/4)
SfuI 330 TTCGAA TT^CGAA
Tth111I 178 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1097 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 111 CCATTATAACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AhdI 859 GACAATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1287 GACAGGAAGTC
AlwNI 1317 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
1874 CAGGATCTG
AspEI 859 GACAATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1287 GACAGGAAGTC
BclI 818 TGATCA T^GATCA
1389 TGATCA
BsaWI 255 ACCGGA [AT]^CCGG[AT]
1338 ACCGGT
BseRI 277 CTCCTC GAGGAG(10/8)
10 GAGGAG
BsgI 440 GTGCAG GTGCAG(16/14)
1424 GTGCAG
BsmAI 1285 GAGAC GTCTC(1/5)
1525 GAGAC
BstXI 120 CCATGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
379 CCATTGCACTGG
Eam1105I 859 GACAATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1287 GACAGGAAGTC
FauI 6 GCGGG CCCGC(4/6)
64 GCGGG
FbaI 818 TGATCA T^GATCA
1389 TGATCA
MwoI 694 GCAGTGCTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
838 GCAAGGCAAGC
SfaNI 754 GATGC GCATC(5/9)
1802 GATGC
SfcI 1019 CTACAG C^T[GA][CT]AG
1314 CTGCAG
SspI 970 AATATT AAT^ATT
1777 AATATT
TfiI 775 GAATC G^A[AT]TC
976 GATTC
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BsaJI
BsiYI BslI BsmFI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI NcoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII