Restriction map for pFN21AA0906
 (Circular) (Six-base) MAPSORT of: pFN21AA0906.seq  Check: 2673  from: 1  to: 10366



pFN21AA0906  10366 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:31  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   4318    5476    7033    4318 
   Size:       1158    1557    7651

  Fragments arranged by size: 

                7651    1557    1158
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2757    5661    8176     278 
   Size:         53      83     186     936    1221    2904    2515    2468

  Fragments arranged by size: 

                2904    2515    2468    1221     936     186      83      53
            
 
AccI GT'mk_AC

Cuts at:   1937    2558    2682    2757    4275    7709    1937 
   Size:        621     124      75    1518    3434    4594

  Fragments arranged by size: 

                4594    3434    1518     621     124      75
 
Acc65I G'GTAC_C

Cuts at:   7687    7687 
   Size:      10366
 
AclI AA'CG_TT

Cuts at:   1563    8493    8866    1563 
   Size:       6930     373    3063

  Fragments arranged by size: 

                6930    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2434    2435    2843    3442    4393    5081    7048    8429 
   Size:        802       1     408     599     951     688    1967    1381

Cuts at:   8429    9813   10081    1632 
   Size:       1384     268    1917

  Fragments arranged by size: 

                1967    1917    1384    1381     951     802     688     599
                 408     268       1
 
AfeI AGC'GCT

Cuts at:   5374    5374 
   Size:      10366
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197   10169
 
AflIII A'CryG_T

Cuts at:   1203    2922    3646    4296    4926    9265   10242    1203 
   Size:       1719     724     650     630    4339     977    1327

  Fragments arranged by size: 

                4339    1719    1327     977     724     650     630
 
AgeI A'CCGG_T

Cuts at:   9197    9197 
   Size:      10366
 
AhdI GACnn_n'nnGTC

Cuts at:   9095    9095 
   Size:      10366
 
AleI CACnn'nnGTG

Cuts at:   1166    3066    3284    3897    1166 
   Size:       1900     218     613    7635

  Fragments arranged by size: 

                7635    1900     613     218
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    4376    5573    5633    1184 
   Size:       3192    1197      60    5917

  Fragments arranged by size: 

                5917    3192    1197      60
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5585    5617    5585 
   Size:         32   10334
 
AlwNI CAG_nnn'CTG

Cuts at:   2323    2428    3497    3661    3809    4945    8109    9681    2323 
   Size:        105    1069     164     148    1136    3164    1572    3008

  Fragments arranged by size: 

                3164    3008    1572    1136    1069     164     148     105
            
 
ApaI G_GGCC'C

Cuts at:   2066    2879    3439    6320    7380    2066 
   Size:        813     560    2881    1060    5052

  Fragments arranged by size: 

                5052    2881    1060     813     560
 
ApaLI G'TGCA_C

Cuts at:   4841    6778    8423    9579    4841 
   Size:       1937    1645    1156    5628

  Fragments arranged by size: 

                5628    1937    1645    1156
 
ApoI r'AATT_y

Cuts at:   1493    3351    4034    7675    7901    8053   10162    1493 
   Size:       1858     683    3641     226     152    2109    1697

  Fragments arranged by size: 

                3641    2109    1858    1697     683     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    5900    8920     160 
   Size:        872    4868    3020    1606

  Fragments arranged by size: 

                4868    3020    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      10366
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2872    4022    4855    6394    6585    7506    7539 
   Size:         73     929    1150     833    1539     191     921      33

Cuts at:   7539    7691    1870 
   Size:        152    4545

  Fragments arranged by size: 

                4545    1539    1150     929     921     833     191     152
                  73      33
 
AvrII C'CTAG_G

Cuts at:   3046    3046 
   Size:      10366
 
BamHI G'GATC_C

Cuts at:   3943    4017    4125    5517    6596    7696    8063    3943 
   Size:         74     108    1392    1079    1100     367    6246

  Fragments arranged by size: 

                6246    1392    1100    1079     367     108      74
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3322    3413    4645    5549    5679 
   Size:        325     217     627    1535      91    1232     904     130

Cuts at:   5679    6627    7057    7142    7687    9142     618 
   Size:        948     430      85     545    1455    1842

  Fragments arranged by size: 

                1842    1535    1455    1232     948     904     627     545
                 430     325     217     130      91      85
 
BanII G_rGCy'C

Cuts at:   1391    2066    2388    2651    2879    3439    3609    3854    3977 
   Size:        675     322     263     228     560     170     245     123

Cuts at:   3977    4671    4700    4746    4862    5427    6078    6152    6320 
   Size:        694      29      46     116     565     651      74     168

Cuts at:   6320    6533    7380    7649    7685    1391 
   Size:        213     847     269      36    4072

  Fragments arranged by size: 

                4072     847     694     675     651     565     560     322
                 269     263     245     228     213     170     168     123
                 116      74      46      36      29
 
BbeI G_GCGC'C

Cuts at:   3417    3417 
   Size:      10366
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2464    2645    3459    4888    4988    6241    6995 
   Size:        971     565     181     814    1429     100    1253     754

Cuts at:   6995     928 
   Size:       4299

  Fragments arranged by size: 

                4299    1429    1253     971     814     754     565     181
                 100
 
BbvCI CC'TCA_GC

Cuts at:   4056    4607    5762    4056 
   Size:        551    1155    8660

  Fragments arranged by size: 

                8660    1155     551
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2275    2309    3511    3545    6047    6081    8557 
   Size:         34     338      34    1202      34    2502      34    2476

Cuts at:   8557    8591    1903 
   Size:         34    3678

  Fragments arranged by size: 

                3678    2502    2476    1202     338      34      34      34
                  34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3035    3384    6127    8258    9468    3035 
   Size:        349    2743    2131    1210    3933

  Fragments arranged by size: 

                3933    2743    2131    1210     349
 
BclI T'GATC_A

Cuts at:   1548    7476    1548 
   Size:       5928    4438
 
BfrBI ATG'CAT

Cuts at:   7601    7601 
   Size:      10366
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    2078    6185 
   Size:        107     122      71     631     605     337      69    4107

Cuts at:   6185    6510    7088    7196    7457    8977     136 
   Size:        325     578     108     261    1520    1525

  Fragments arranged by size: 

                4107    1525    1520     631     605     578     337     325
                 261     122     108     107      71      69
 
BglII A'GATC_T

Cuts at:  10361   10361 
   Size:      10366
 
BlpI GC'TnA_GC

Cuts at:   4467    7783    4467 
   Size:       3316    7050
 
Bme1580I G_kGCm'C

Cuts at:   1790    2066    2879    3244    3439    4845    5552    6320    6630 
   Size:        276     813     365     195    1406     707     768     310

Cuts at:   6630    6782    7062    7147    7380    8427    9583    1790 
   Size:        152     280      85     233    1047    1156    2573

  Fragments arranged by size: 

                2573    1406    1156    1047     813     768     707     365
                 310     280     276     233     195     152      85
 
BmgBI CAC'GTC

Cuts at:   1318    2925    3889    4687    6475    1318 
   Size:       1607     964     798    1788    5209

  Fragments arranged by size: 

                5209    1788    1607     964     798
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4331    6838    7337 
   Size:        416      53      83     399      24    2914    2507     499

Cuts at:   7337    8097    9055     442 
   Size:        760     958    1753

  Fragments arranged by size: 

                2914    2507    1753     958     760     499     416     399
                  83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:      10366
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3220    7415    3220 
   Size:       4195    6171
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2165    2414    2429    3104    3683    6261    6547    7003 
   Size:        926     249      15     675     579    2578     286     456

Cuts at:   7003    7178    7294    9026    1239 
   Size:        175     116    1732    2579

  Fragments arranged by size: 

                2579    2578    1732     926     675     579     456     286
                 249     175     116      15
 
Bpu10I CC'TnA_GC

Cuts at:   4056    4607    4762    5504    5762    6977    4056 
   Size:        551     155     742     258    1215    7445

  Fragments arranged by size: 

                7445    1215     742     551     258     155
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2642    3742    3917    6861    8491    9356    9654    2642 
   Size:       1100     175    2944    1630     865     298    3354

  Fragments arranged by size: 

                3354    2944    1630    1100     865     298     175
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2880    3080    5441    6815    7079    9029     882 
   Size:        617    1381     200    2361    1374     264    1950    2219

  Fragments arranged by size: 

                2361    2219    1950    1381    1374     617     264     200
            
 
BsaAI yAC'GTr

Cuts at:    493    1204    2562    3028    3647    4927    8157     493 
   Size:        711    1358     466     619    1280    3230    2702

  Fragments arranged by size: 

                3230    2702    1358    1280     711     619     466
 
BsaBI GATnn'nnATC

Cuts at:   5561   10360    5561 
   Size:       4799    5567
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2754    3414    3825    5658 
   Size:         53      83     186     936    1221     660     411    1833

Cuts at:   5658    5690    7254    8173    8555    9190     275 
   Size:         32    1564     919     382     635    1451

  Fragments arranged by size: 

                1833    1564    1451    1221     936     919     660     635
                 411     382     186      83      53      32
 
BsaWI w'CCGG_w

Cuts at:   8799    9197    9471    9618   10029    8799 
   Size:        398     274     147     411    9136

  Fragments arranged by size: 

                9136     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2546    2576    3977    4007    5585 
   Size:         30     165      30    1143      30    1401      30    1578

Cuts at:   5585    5615    6887    6917    1178 
   Size:         30    1272      30    4627

  Fragments arranged by size: 

                4627    1578    1401    1272    1143     165      30      30
                  30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3940    3967    6523    1186 
   Size:       2754      27    2556    5029

  Fragments arranged by size: 

                5029    2754    2556      27
 
BseYI C'CCAG_C

Cuts at:   2209    2265    2947    3377    4187    4287    4906    5316    5378 
   Size:         56     682     430     810     100     619     410      62

Cuts at:   5378    6623    7229    7460    7648    9569   10065    2209 
   Size:       1245     606     231     188    1921     496    2510

  Fragments arranged by size: 

                2510    1921    1245     810     682     619     606     496
                 430     410     231     188     100      62      56
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2270    2813    2922    3581    4505    5450    5504 
   Size:        573     534     543     109     659     924     945      54

Cuts at:   5504    5967    6143    6323    7483    1163 
   Size:        463     176     180    1160    4046

  Fragments arranged by size: 

                4046    1160     945     924     659     573     543     534
                 463     180     176     109      54
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2186    2900    3094    5740    6858    7191 
   Size:        275     186     185     714     194    2646    1118     333

Cuts at:   7191    7251    7816    8577    9605    1540 
   Size:         60     565     761    1028    2301

  Fragments arranged by size: 

                2646    2301    1118    1028     761     714     565     333
                 275     194     186     185      60
 
BsiHKAI G_wGCw'C

Cuts at:   2234    2388    2651    3204    3816    4671    4746    4845    5736 
   Size:        154     263     553     612     855      75      99     891

Cuts at:   5736    6393    6480    6533    6782    8427    8512    9583    2234 
   Size:        657      87      53     249    1645      85    1071    3017

  Fragments arranged by size: 

                3017    1645    1071     891     855     657     612     553
                 263     249     154      99      87      85      75      53
            
 
BsmI GAATG_Cn'

Cuts at:   7199    7877    7970    7199 
   Size:        678      93    9595

  Fragments arranged by size: 

                9595     678      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2374    6285    7261    2374 
   Size:       3911     976    5479

  Fragments arranged by size: 

                5479    3911     976
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2066    2234    2388    2651    2879    3204    3244 
   Size:        399     276     168     154     263     228     325      40

Cuts at:   3244    3439    3609    3816    3854    3977    4671    4700    4746 
   Size:        195     170     207      38     123     694      29      46

Cuts at:   4746    4845    4862    5427    5552    5736    6078    6152    6320 
   Size:         99      17     565     125     184     342      74     168

Cuts at:   6320    6393    6480    6533    6630    6782    7062    7147    7380 
   Size:         73      87      53      97     152     280      85     233

Cuts at:   7380    7649    7685    8427    8512    9583    1391 
   Size:        269      36     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     694     565     399     342     325
                 280     276     269     263     233     228     207     195
                 184     170     168     168     154     152     125     123
                  99      97      87      85      85      74      73      53
                  46      40      38      36      29      17
 
BspEI T'CCGG_A

Cuts at:  10029   10029 
   Size:      10366
 
BspHI T'CATG_A

Cuts at:   4872    7473    8255    9985    4872 
   Size:       2601     782    1730    5253

  Fragments arranged by size: 

                5253    2601    1730     782
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3933    4147    4318    5284    5476    7033    7721     844 
   Size:       3089     214     171     966     192    1557     688    3489

  Fragments arranged by size: 

                3489    3089    1557     966     688     214     192     171
            
 
BsrBI CCG'CTC

Cuts at:   1394    1823    8253    1394 
   Size:        429    6430    3507

  Fragments arranged by size: 

                6430    3507     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3912    5356    5617    7332    7601    8861    9035 
   Size:       1183    2664    1444     261    1715     269    1260     174

Cuts at:   9035      65 
   Size:       1396

  Fragments arranged by size: 

                2664    1715    1444    1396    1260    1183     269     261
                 174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2899    3089    4395    5450    5573    6853    9010 
   Size:        110    1089     190    1306    1055     123    1280    2157

Cuts at:   9010    9197    1700 
   Size:        187    2869

  Fragments arranged by size: 

                2869    2157    1306    1280    1089    1055     190     187
                 123     110
 
BsrGI T'GTAC_A

Cuts at:     96    4947      96 
   Size:       4851    5515
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:      10366
 
BssSI C'ACGA_G

Cuts at:   4547    5426    5480    5684    8426    9438    4547 
   Size:        879      54     204    2742    1012    5475

  Fragments arranged by size: 

                5475    2742    1012     879     204      54
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    4528    4849    5441     794 
   Size:       3734     321     592    5719

  Fragments arranged by size: 

                5719    3734     592     321
 
BstBI TT'CG_AA

Cuts at:   9170    9170 
   Size:      10366
 
BstEII G'GTnAC_C

Cuts at:   1184    3006    3715    3964    4708    5296    1184 
   Size:       1822     709     249     744     588    6254

  Fragments arranged by size: 

                6254    1822     744     709     588     249
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3712    4483    6466    3712 
   Size:        771    1983    7612

  Fragments arranged by size: 

                7612    1983     771
 
BstYI r'GATC_y

Cuts at:   1972    2418    3943    4017    4125    5517    5835    6596    7510 
   Size:        446    1525      74     108    1392     318     761     914

Cuts at:   7510    7696    8063    8448    8465    9917   10003   10015   10361 
   Size:        186     367     385      17    1452      86      12     346

Cuts at:  10361    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1525    1452    1392     914     761     446     385
                 367     346     318     186     108      86      74      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   3674    6134    6632    7633    3674 
   Size:       2460     498    1001    6407

  Fragments arranged by size: 

                6407    2460    1001     498
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    3395    3439    4224    4836    5143    6459 
   Size:        552     939    1391      44     785     612     307    1316

Cuts at:   6459    6591    6744    6891    7266    7372     513 
   Size:        132     153     147     375     106    3507

  Fragments arranged by size: 

                3507    1391    1316     939     785     612     552     375
                 307     153     147     132     106      44
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    3215    4147     487 
   Size:        683      56    1989     932    6706

  Fragments arranged by size: 

                6706    1989     932     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2234    2250    2934    2939    3454    3529    3814    4572    4578 
   Size:         16     684       5     515      75     285     758       6

Cuts at:   4578    5270    5442    6365    6909    7882    8676    8696    2234 
   Size:        692     172     923     544     973     794      20    3904

  Fragments arranged by size: 

                3904     973     923     794     758     692     684     544
                 515     285     172      75      20      16       6       5
            
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:      10366
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    3847    3882     529 
   Size:         35    3283      35    7013

  Fragments arranged by size: 

                7013    3283      35      35
 
DraI TTT'AAA

Cuts at:   7670    7670 
   Size:      10366
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3749    9373     809 
   Size:        507    2433    5624    1802

  Fragments arranged by size: 

                5624    2433    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2079    2183    2193 
   Size:         54    1425      50     275      18     249     104      10

Cuts at:   2193    2897    2971    3091    3261    3883    4452    4507    5151 
   Size:        704      74     120     170     622     569      55     644

Cuts at:   5151    5158    5737    5814    6186    6486    6537    6855    6919 
   Size:          7     579      77     372     300      51     318      64

Cuts at:   6919    7188    7813    8702   10331       8 
   Size:        269     625     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     704     644     625     622     579
                 569     372     318     300     275     269     249     170
                 120     104      77      74      64      55      54      51
                  50      43      18      10       7
 
EagI C'GGCC_G

Cuts at:   1537    1812    2183    2897    3091    5737    6855    7188    7813 
   Size:        275     371     714     194    2646    1118     333     625

Cuts at:   7813    1537 
   Size:       4090

  Fragments arranged by size: 

                4090    2646    1118     714     625     371     333     275
                 194
 
EarI CTCTTCn'nnn_

Cuts at:   1353    4698    7082    7406    7451    8296    1353 
   Size:       3345    2384     324      45     845    3423

  Fragments arranged by size: 

                3423    3345    2384     845     324      45
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2059    2519    8953    9327    9473     387 
   Size:       1064     138     470     460    6434     374     146    1280

  Fragments arranged by size: 

                6434    1280    1064     470     460     374     146     138
            
 
EcoICRI GAG'CTC

Cuts at:   2386    2649    4669    4744    6531    2386 
   Size:        263    2020      75    1787    6221

  Fragments arranged by size: 

                6221    2020    1787     263      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2165    2414    2429    2434    2435    2843    3104 
   Size:        393     533     249      15       5       1     408     261

Cuts at:   3104    3442    3683    4393    5081    6261    6547    7003    7048 
   Size:        338     241     710     688    1180     286     456      45

Cuts at:   7048    7178    7294    8429    9026    9813   10081    1239 
   Size:        130     116    1135     597     787     268    1524

  Fragments arranged by size: 

                1524    1180    1135     787     710     688     597     533
                 456     408     393     338     286     268     261     249
                 241     130     116      45      15       5       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:   4679    5483    6133    7632    4679 
   Size:        804     650    1499    7413

  Fragments arranged by size: 

                7413    1499     804     650
 
EcoO109I rG'GnC_Cy

Cuts at:   2063    2320    2425    2876    3435    3658    4004    5392    5476 
   Size:        257     105     451     559     223     346    1388      84

Cuts at:   5476    6178    6316    6317    6636    7182    7283    2063 
   Size:        702     138       1     319     546     101    5146

  Fragments arranged by size: 

                5146    1388     702     559     546     451     346     319
                 257     223     138     105     101      84       1
 
EcoRI G'AATT_C

Cuts at:   7675    8053    7675 
   Size:        378    9988
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   5044    5044 
   Size:      10366
 
FspI TGC'GCA

Cuts at:   8872    8872 
   Size:      10366
 
HaeII r_GCGC'y

Cuts at:   1678    3417    5376    9513    1678 
   Size:       1739    1959    4137    2531

  Fragments arranged by size: 

                4137    2531    1959    1739
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2498    2530    3935    3967    4002 
   Size:         32     135      32     926      32    1405      32      35

Cuts at:   4002    4034    5190    5222    5777    5809    6743    6775    6857 
   Size:         32    1156      32     555      32     934      32      82

Cuts at:   6857    6889    7160    7192    9007    9039    9081    9113    1373 
   Size:         32     271      32    1815      32      42      32    2626

  Fragments arranged by size: 

                2626    1815    1405    1156     934     926     555     271
                 135      82      42      35      32      32      32      32
                  32      32      32      32      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2134    4963    7710    7956     669 
   Size:        988     281     196    2829    2747     246    3079

  Fragments arranged by size: 

                3079    2829    2747     988     281     246     196
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:      10366
 
HpaI GTT'AAC

Cuts at:   2134    7956    2134 
   Size:       5822    4544
 
KasI G'GCGC_C

Cuts at:   3413    3413 
   Size:      10366
 
KpnI G_GTAC'C

Cuts at:   7691    7691 
   Size:      10366
 
MfeI C'AATT_G

Cuts at:   7965    7965 
   Size:      10366
 
MluI A'CGCG_T

Cuts at:  10242   10242 
   Size:      10366
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4911    5352    5722    9480    9664    1305 
   Size:       3606     441     370    3758     184    2007

  Fragments arranged by size: 

                3758    3606    2007     441     370     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2195    2973    4454    4509    5153 
   Size:         54    1425     343     363     778    1481      55     644

Cuts at:   5153    5816    6488      10 
   Size:        663     672    3888

  Fragments arranged by size: 

                3888    1481    1425     778     672     663     644     363
                 343      55      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2624    2927    3066    3284    3394    3579    3897 
   Size:        648    1458     303     139     218     110     185     318

Cuts at:   3897    4676    4871    4955    5537    5555    5729    6006    6059 
   Size:        779     195      84     582      18     174     277      53

Cuts at:   6059    6464    7343    8324    8683    8842     518 
   Size:        405     879     981     359     159    2042

  Fragments arranged by size: 

                2042    1458     981     879     779     648     582     405
                 359     318     303     277     218     195     185     174
                 159     139     110      84      53      18
 
MspA1I CmG'CkG

Cuts at:   1606    2075    2169    2209    2947    3462    3822    4055    4291 
   Size:        469      94      40     738     515     360     233     236

Cuts at:   4291    4309    4483    5145    5803    5866    6834    6967    7268 
   Size:         18     174     662     658      63     968     133     301

Cuts at:   7268    7469    7782    8459    9607    9852    1606 
   Size:        201     313     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     968     738     677     662     658     515
                 469     360     313     301     245     236     233     201
                 174     133      94      63      40      18
 
NaeI GCC'GGC

Cuts at:   6855    6855 
   Size:      10366
 
NarI GG'CG_CC

Cuts at:   3414    3414 
   Size:      10366
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    4836    6459     513 
   Size:        552     939    2832    1623    4420

  Fragments arranged by size: 

                4420    2832    1623     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:      10366
 
NgoMIV G'CCGG_C

Cuts at:   6853    6853 
   Size:      10366
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:      10366
 
NotI GC'GGCC_GC

Cuts at:   7813    7813 
   Size:      10366
 
NsiI A_TGCA'T

Cuts at:   7603    7603 
   Size:      10366
 
NspI r_CATG'y

Cuts at:   1132    1229    2992    4300    6005    7724    9269    1132 
   Size:         97    1763    1308    1705    1719    1545    2229

  Fragments arranged by size: 

                2229    1763    1719    1705    1545    1308      97
 
PasI CC'CwG_GG

Cuts at:   1348    3788    3847    3970    1348 
   Size:       2440      59     123    7744

  Fragments arranged by size: 

                7744    2440     123      59
 
PciI A'CATG_T

Cuts at:   4296    9265    4296 
   Size:       4969    5397
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2428    3616    3661    4014    1108 
   Size:       1320    1188      45     353    7460

  Fragments arranged by size: 

                7460    1320    1188     353      45
 
PfoI T'CCnGG_A

Cuts at:   2670    4103    5661    5970    6711    7413    2670 
   Size:       1433    1558     309     741     702    5623

  Fragments arranged by size: 

                5623    1558    1433     741     702     309
 
PmeI GTTT'AAAC

Cuts at:   7670    7670 
   Size:      10366
 
PmlI CAC'GTG

Cuts at:   3647    3647 
   Size:      10366
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5585    5617    8435    8467    9948    9980    5585 
   Size:         32    2818      32    1481      32    5971

  Fragments arranged by size: 

                5971    2818    1481      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2320    4004    5476    6636    7182    2320 
   Size:       1684    1472    1160     546    5504

  Fragments arranged by size: 

                5504    1684    1472    1160     546
 
PshAI GACnn'nnGTC

Cuts at:   1613    5704    7469    1613 
   Size:       4091    1765    4510

  Fragments arranged by size: 

                4510    4091    1765
 
PsiI TTA'TAA

Cuts at:   7936    7936 
   Size:      10366
 
PspOMI G'GGCC_C

Cuts at:   2062    2875    3435    6316    7376    2062 
   Size:        813     560    2881    1060    5052

  Fragments arranged by size: 

                5052    2881    1060     813     560
 
PspXI vC'TCGA_Gb

Cuts at:   4022    7539    4022 
   Size:       3517    6849
 
PstI C_TGCA'G

Cuts at:    830    2704    2946    3821    4317    5865    5931    7528    7718 
   Size:       1874     242     875     496    1548      66    1597     190

Cuts at:   7718     830 
   Size:       3478

  Fragments arranged by size: 

                3478    1874    1597    1548     875     496     242     190
                  66
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:      10366
 
PvuII CAG'CTG

Cuts at:   2947    3462    4291    4483    5803    5866    6834    2947 
   Size:        515     829     192    1320      63     968    6479

  Fragments arranged by size: 

                6479    1320     968     829     515     192      63
 
RsrII CG'GwC_CG

Cuts at:   5754    5754 
   Size:      10366
 
SacI G_AGCT'C

Cuts at:   2388    2651    4671    4746    6533    2388 
   Size:        263    2020      75    1787    6221

  Fragments arranged by size: 

                6221    2020    1787     263      75
 
SacII CC_GC'GG

Cuts at:   5146    7269    5146 
   Size:       2123    8243
 
SalI G'TCGA_C

Cuts at:   1936    7708    1936 
   Size:       5772    4594
 
SanDI GG'GwC_CC

Cuts at:   7182    7182 
   Size:      10366
 
SapI GCTCTTCn'nnn_

Cuts at:   7451    7451 
   Size:      10366
 
SbfI CC_TGCA'GG

Cuts at:   7718    7718 
   Size:      10366
 
ScaI AGT'ACT

Cuts at:   6926    8614   10326    6926 
   Size:       1688    1712    6966

  Fragments arranged by size: 

                6966    1712    1688
 
SexAI A'CCwGG_T

Cuts at:   3480    7425    3480 
   Size:       3945    6421
 
SfcI C'TryA_G

Cuts at:    826    1045    2700    2942    3317    3817    3874    4313    5861 
   Size:        219    1655     242     375     500      57     439    1548

Cuts at:   5861    5927    6428    6720    7524    7714    8849    9530    9721 
   Size:         66     501     292     804     190    1135     681     191

Cuts at:   9721     826 
   Size:       1471

  Fragments arranged by size: 

                1655    1548    1471    1135     804     681     501     500
                 439     375     292     242     219     191     190      66
                  57
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   6185    6185 
   Size:      10366
 
SfoI GGC'GCC

Cuts at:   3415    3415 
   Size:      10366
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      10366
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:      10366
 
SmaI CCC'GGG

Cuts at:   1872    2874    4857    6396    6587    7508    7693    1872 
   Size:       1002    1983    1539     191     921     185    4545

  Fragments arranged by size: 

                4545    1983    1539    1002     921     191     185
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2657    3721    3932    4022    6876    7539 
   Size:        197     926     714    1064     211      90    2854     663

Cuts at:   7539    8470    9371    9633     820 
   Size:        931     901     262    1553

  Fragments arranged by size: 

                2854    1553    1064     931     926     901     714     663
                 262     211     197      90
 
SnaBI TAC'GTA

Cuts at:    493    2562    8157     493 
   Size:       2069    5595    2702

  Fragments arranged by size: 

                5595    2702    2069
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:      10366
 
SphI G_CATG'C

Cuts at:   1132    2992    7724    1132 
   Size:       1860    4732    3774

  Fragments arranged by size: 

                4732    3774    1860
 
SrfI GCCC'GGGC

Cuts at:   6587    6587 
   Size:      10366
 
SspI AAT'ATT

Cuts at:      5      52    5898    8290       5 
   Size:         47    5846    2392    2081

  Fragments arranged by size: 

                5846    2392    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2150    4145    5625    6513    6809    1517 
   Size:        633    1995    1480     888     296    5074

  Fragments arranged by size: 

                5074    1995    1480     888     633     296
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2968    3046    3960    4013    4146    4836 
   Size:        552     939     964      78     914      53     133     690

Cuts at:   4836    4911    6459    7279    7805     513 
   Size:         75    1548     820     526    3074

  Fragments arranged by size: 

                3074    1548     964     939     914     820     690     552
                 526     133      78      75      53
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3067    4139    5567    6179    6256    6803    6831 
   Size:       1255    1487    1072    1428     612      77     547      28

Cuts at:   6831    7212    7600    8393    8410    8563    8748    9193     325 
   Size:        381     388     793      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1487    1428    1255    1072     793     612     547
                 445     388     381     185     153      77      28      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3423    4947    5712 
   Size:        274      80      33      51    1443    1446    1524     765

Cuts at:   5712    6924    8612   10324      96 
   Size:       1212    1688    1712     138

  Fragments arranged by size: 

                1712    1688    1524    1446    1443    1212     765     274
                 138      80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3358    3390    6192    6224   10326   10358    3358 
   Size:         32    2802      32    4102      32    3366

  Fragments arranged by size: 

                4102    3366    2802      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2041    1316 
   Size:        725    9641
 
XbaI T'CTAG_A

Cuts at:   7702    7702 
   Size:      10366
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3446    4288    4447    6236    6571    7042    3446 
   Size:        842     159    1789     335     471    6770

  Fragments arranged by size: 

                6770    1789     842     471     335     159
 
XhoI C'TCGA_G

Cuts at:   1943    4022    7539    1943 
   Size:       2079    3517    4770

  Fragments arranged by size: 

                4770    3517    2079
 
XmaI C'CCGG_G

Cuts at:   1870    2872    4855    6394    6585    7506    7691    1870 
   Size:       1002    1983    1539     191     921     185    4545

  Fragments arranged by size: 

                4545    1983    1539    1002     921     191     185
 
XmnI GAAnn'nnTTC

Cuts at:   2447    5027    5235    5597    5988    8495    2447 
   Size:       2580     208     362     391    2507    4318

  Fragments arranged by size: 

                4318    2580    2507     391     362     208
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2755    5659    8174     276 
   Size:         53      83     186     936    1221    2904    2515    2468

  Fragments arranged by size: 

                2904    2515    2468    1221     936     186      83      53
            
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NsiI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AscI     BaeI    BsiWI  BstZ17I   DraIII    EcoRV     FseI    FspAI
     NruI     PacI     PsrI     SwaI
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