(Circular) (Six-base) MAPSORT of: pFN21AA0906.seq Check: 2673 from: 1 to: 10366 pFN21AA0906 10366 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4318 5476 7033 4318 Size: 1158 1557 7651 Fragments arranged by size: 7651 1557 1158 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2757 5661 8176 278 Size: 53 83 186 936 1221 2904 2515 2468 Fragments arranged by size: 2904 2515 2468 1221 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2558 2682 2757 4275 7709 1937 Size: 621 124 75 1518 3434 4594 Fragments arranged by size: 4594 3434 1518 621 124 75 Acc65I G'GTAC_C Cuts at: 7687 7687 Size: 10366 AclI AA'CG_TT Cuts at: 1563 8493 8866 1563 Size: 6930 373 3063 Fragments arranged by size: 6930 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2434 2435 2843 3442 4393 5081 7048 8429 Size: 802 1 408 599 951 688 1967 1381 Cuts at: 8429 9813 10081 1632 Size: 1384 268 1917 Fragments arranged by size: 1967 1917 1384 1381 951 802 688 599 408 268 1 AfeI AGC'GCT Cuts at: 5374 5374 Size: 10366 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10169 AflIII A'CryG_T Cuts at: 1203 2922 3646 4296 4926 9265 10242 1203 Size: 1719 724 650 630 4339 977 1327 Fragments arranged by size: 4339 1719 1327 977 724 650 630 AgeI A'CCGG_T Cuts at: 9197 9197 Size: 10366 AhdI GACnn_n'nnGTC Cuts at: 9095 9095 Size: 10366 AleI CACnn'nnGTG Cuts at: 1166 3066 3284 3897 1166 Size: 1900 218 613 7635 Fragments arranged by size: 7635 1900 613 218 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4376 5573 5633 1184 Size: 3192 1197 60 5917 Fragments arranged by size: 5917 3192 1197 60 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5585 5617 5585 Size: 32 10334 AlwNI CAG_nnn'CTG Cuts at: 2323 2428 3497 3661 3809 4945 8109 9681 2323 Size: 105 1069 164 148 1136 3164 1572 3008 Fragments arranged by size: 3164 3008 1572 1136 1069 164 148 105 ApaI G_GGCC'C Cuts at: 2066 2879 3439 6320 7380 2066 Size: 813 560 2881 1060 5052 Fragments arranged by size: 5052 2881 1060 813 560 ApaLI G'TGCA_C Cuts at: 4841 6778 8423 9579 4841 Size: 1937 1645 1156 5628 Fragments arranged by size: 5628 1937 1645 1156 ApoI r'AATT_y Cuts at: 1493 3351 4034 7675 7901 8053 10162 1493 Size: 1858 683 3641 226 152 2109 1697 Fragments arranged by size: 3641 2109 1858 1697 683 226 152 AseI AT'TA_AT Cuts at: 160 1032 5900 8920 160 Size: 872 4868 3020 1606 Fragments arranged by size: 4868 3020 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10366 AvaI C'yCGr_G Cuts at: 1870 1943 2872 4022 4855 6394 6585 7506 7539 Size: 73 929 1150 833 1539 191 921 33 Cuts at: 7539 7691 1870 Size: 152 4545 Fragments arranged by size: 4545 1539 1150 929 921 833 191 152 73 33 AvrII C'CTAG_G Cuts at: 3046 3046 Size: 10366 BamHI G'GATC_C Cuts at: 3943 4017 4125 5517 6596 7696 8063 3943 Size: 74 108 1392 1079 1100 367 6246 Fragments arranged by size: 6246 1392 1100 1079 367 108 74 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3322 3413 4645 5549 5679 Size: 325 217 627 1535 91 1232 904 130 Cuts at: 5679 6627 7057 7142 7687 9142 618 Size: 948 430 85 545 1455 1842 Fragments arranged by size: 1842 1535 1455 1232 948 904 627 545 430 325 217 130 91 85 BanII G_rGCy'C Cuts at: 1391 2066 2388 2651 2879 3439 3609 3854 3977 Size: 675 322 263 228 560 170 245 123 Cuts at: 3977 4671 4700 4746 4862 5427 6078 6152 6320 Size: 694 29 46 116 565 651 74 168 Cuts at: 6320 6533 7380 7649 7685 1391 Size: 213 847 269 36 4072 Fragments arranged by size: 4072 847 694 675 651 565 560 322 269 263 245 228 213 170 168 123 116 74 46 36 29 BbeI G_GCGC'C Cuts at: 3417 3417 Size: 10366 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2464 2645 3459 4888 4988 6241 6995 Size: 971 565 181 814 1429 100 1253 754 Cuts at: 6995 928 Size: 4299 Fragments arranged by size: 4299 1429 1253 971 814 754 565 181 100 BbvCI CC'TCA_GC Cuts at: 4056 4607 5762 4056 Size: 551 1155 8660 Fragments arranged by size: 8660 1155 551 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2275 2309 3511 3545 6047 6081 8557 Size: 34 338 34 1202 34 2502 34 2476 Cuts at: 8557 8591 1903 Size: 34 3678 Fragments arranged by size: 3678 2502 2476 1202 338 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3035 3384 6127 8258 9468 3035 Size: 349 2743 2131 1210 3933 Fragments arranged by size: 3933 2743 2131 1210 349 BclI T'GATC_A Cuts at: 1548 7476 1548 Size: 5928 4438 BfrBI ATG'CAT Cuts at: 7601 7601 Size: 10366 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2078 6185 Size: 107 122 71 631 605 337 69 4107 Cuts at: 6185 6510 7088 7196 7457 8977 136 Size: 325 578 108 261 1520 1525 Fragments arranged by size: 4107 1525 1520 631 605 578 337 325 261 122 108 107 71 69 BglII A'GATC_T Cuts at: 10361 10361 Size: 10366 BlpI GC'TnA_GC Cuts at: 4467 7783 4467 Size: 3316 7050 Bme1580I G_kGCm'C Cuts at: 1790 2066 2879 3244 3439 4845 5552 6320 6630 Size: 276 813 365 195 1406 707 768 310 Cuts at: 6630 6782 7062 7147 7380 8427 9583 1790 Size: 152 280 85 233 1047 1156 2573 Fragments arranged by size: 2573 1406 1156 1047 813 768 707 365 310 280 276 233 195 152 85 BmgBI CAC'GTC Cuts at: 1318 2925 3889 4687 6475 1318 Size: 1607 964 798 1788 5209 Fragments arranged by size: 5209 1788 1607 964 798 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4331 6838 7337 Size: 416 53 83 399 24 2914 2507 499 Cuts at: 7337 8097 9055 442 Size: 760 958 1753 Fragments arranged by size: 2914 2507 1753 958 760 499 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10366 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3220 7415 3220 Size: 4195 6171 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2165 2414 2429 3104 3683 6261 6547 7003 Size: 926 249 15 675 579 2578 286 456 Cuts at: 7003 7178 7294 9026 1239 Size: 175 116 1732 2579 Fragments arranged by size: 2579 2578 1732 926 675 579 456 286 249 175 116 15 Bpu10I CC'TnA_GC Cuts at: 4056 4607 4762 5504 5762 6977 4056 Size: 551 155 742 258 1215 7445 Fragments arranged by size: 7445 1215 742 551 258 155 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2642 3742 3917 6861 8491 9356 9654 2642 Size: 1100 175 2944 1630 865 298 3354 Fragments arranged by size: 3354 2944 1630 1100 865 298 175 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2880 3080 5441 6815 7079 9029 882 Size: 617 1381 200 2361 1374 264 1950 2219 Fragments arranged by size: 2361 2219 1950 1381 1374 617 264 200 BsaAI yAC'GTr Cuts at: 493 1204 2562 3028 3647 4927 8157 493 Size: 711 1358 466 619 1280 3230 2702 Fragments arranged by size: 3230 2702 1358 1280 711 619 466 BsaBI GATnn'nnATC Cuts at: 5561 10360 5561 Size: 4799 5567 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2754 3414 3825 5658 Size: 53 83 186 936 1221 660 411 1833 Cuts at: 5658 5690 7254 8173 8555 9190 275 Size: 32 1564 919 382 635 1451 Fragments arranged by size: 1833 1564 1451 1221 936 919 660 635 411 382 186 83 53 32 BsaWI w'CCGG_w Cuts at: 8799 9197 9471 9618 10029 8799 Size: 398 274 147 411 9136 Fragments arranged by size: 9136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2546 2576 3977 4007 5585 Size: 30 165 30 1143 30 1401 30 1578 Cuts at: 5585 5615 6887 6917 1178 Size: 30 1272 30 4627 Fragments arranged by size: 4627 1578 1401 1272 1143 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3940 3967 6523 1186 Size: 2754 27 2556 5029 Fragments arranged by size: 5029 2754 2556 27 BseYI C'CCAG_C Cuts at: 2209 2265 2947 3377 4187 4287 4906 5316 5378 Size: 56 682 430 810 100 619 410 62 Cuts at: 5378 6623 7229 7460 7648 9569 10065 2209 Size: 1245 606 231 188 1921 496 2510 Fragments arranged by size: 2510 1921 1245 810 682 619 606 496 430 410 231 188 100 62 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2270 2813 2922 3581 4505 5450 5504 Size: 573 534 543 109 659 924 945 54 Cuts at: 5504 5967 6143 6323 7483 1163 Size: 463 176 180 1160 4046 Fragments arranged by size: 4046 1160 945 924 659 573 543 534 463 180 176 109 54 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2186 2900 3094 5740 6858 7191 Size: 275 186 185 714 194 2646 1118 333 Cuts at: 7191 7251 7816 8577 9605 1540 Size: 60 565 761 1028 2301 Fragments arranged by size: 2646 2301 1118 1028 761 714 565 333 275 194 186 185 60 BsiHKAI G_wGCw'C Cuts at: 2234 2388 2651 3204 3816 4671 4746 4845 5736 Size: 154 263 553 612 855 75 99 891 Cuts at: 5736 6393 6480 6533 6782 8427 8512 9583 2234 Size: 657 87 53 249 1645 85 1071 3017 Fragments arranged by size: 3017 1645 1071 891 855 657 612 553 263 249 154 99 87 85 75 53 BsmI GAATG_Cn' Cuts at: 7199 7877 7970 7199 Size: 678 93 9595 Fragments arranged by size: 9595 678 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2374 6285 7261 2374 Size: 3911 976 5479 Fragments arranged by size: 5479 3911 976 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2234 2388 2651 2879 3204 3244 Size: 399 276 168 154 263 228 325 40 Cuts at: 3244 3439 3609 3816 3854 3977 4671 4700 4746 Size: 195 170 207 38 123 694 29 46 Cuts at: 4746 4845 4862 5427 5552 5736 6078 6152 6320 Size: 99 17 565 125 184 342 74 168 Cuts at: 6320 6393 6480 6533 6630 6782 7062 7147 7380 Size: 73 87 53 97 152 280 85 233 Cuts at: 7380 7649 7685 8427 8512 9583 1391 Size: 269 36 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 694 565 399 342 325 280 276 269 263 233 228 207 195 184 170 168 168 154 152 125 123 99 97 87 85 85 74 73 53 46 40 38 36 29 17 BspEI T'CCGG_A Cuts at: 10029 10029 Size: 10366 BspHI T'CATG_A Cuts at: 4872 7473 8255 9985 4872 Size: 2601 782 1730 5253 Fragments arranged by size: 5253 2601 1730 782 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3933 4147 4318 5284 5476 7033 7721 844 Size: 3089 214 171 966 192 1557 688 3489 Fragments arranged by size: 3489 3089 1557 966 688 214 192 171 BsrBI CCG'CTC Cuts at: 1394 1823 8253 1394 Size: 429 6430 3507 Fragments arranged by size: 6430 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3912 5356 5617 7332 7601 8861 9035 Size: 1183 2664 1444 261 1715 269 1260 174 Cuts at: 9035 65 Size: 1396 Fragments arranged by size: 2664 1715 1444 1396 1260 1183 269 261 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2899 3089 4395 5450 5573 6853 9010 Size: 110 1089 190 1306 1055 123 1280 2157 Cuts at: 9010 9197 1700 Size: 187 2869 Fragments arranged by size: 2869 2157 1306 1280 1089 1055 190 187 123 110 BsrGI T'GTAC_A Cuts at: 96 4947 96 Size: 4851 5515 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10366 BssSI C'ACGA_G Cuts at: 4547 5426 5480 5684 8426 9438 4547 Size: 879 54 204 2742 1012 5475 Fragments arranged by size: 5475 2742 1012 879 204 54 BstAPI GCAn_nnn'nTGC Cuts at: 794 4528 4849 5441 794 Size: 3734 321 592 5719 Fragments arranged by size: 5719 3734 592 321 BstBI TT'CG_AA Cuts at: 9170 9170 Size: 10366 BstEII G'GTnAC_C Cuts at: 1184 3006 3715 3964 4708 5296 1184 Size: 1822 709 249 744 588 6254 Fragments arranged by size: 6254 1822 744 709 588 249 BstXI CCAn_nnnn'nTGG Cuts at: 3712 4483 6466 3712 Size: 771 1983 7612 Fragments arranged by size: 7612 1983 771 BstYI r'GATC_y Cuts at: 1972 2418 3943 4017 4125 5517 5835 6596 7510 Size: 446 1525 74 108 1392 318 761 914 Cuts at: 7510 7696 8063 8448 8465 9917 10003 10015 10361 Size: 186 367 385 17 1452 86 12 346 Cuts at: 10361 1972 Size: 1977 Fragments arranged by size: 1977 1525 1452 1392 914 761 446 385 367 346 318 186 108 86 74 17 12 Bsu36I CC'TnA_GG Cuts at: 3674 6134 6632 7633 3674 Size: 2460 498 1001 6407 Fragments arranged by size: 6407 2460 1001 498 BtgI C'CryG_G Cuts at: 513 1065 2004 3395 3439 4224 4836 5143 6459 Size: 552 939 1391 44 785 612 307 1316 Cuts at: 6459 6591 6744 6891 7266 7372 513 Size: 132 153 147 375 106 3507 Fragments arranged by size: 3507 1391 1316 939 785 612 552 375 307 153 147 132 106 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3215 4147 487 Size: 683 56 1989 932 6706 Fragments arranged by size: 6706 1989 932 683 56 BtsI GCAGTG_nn' Cuts at: 2234 2250 2934 2939 3454 3529 3814 4572 4578 Size: 16 684 5 515 75 285 758 6 Cuts at: 4578 5270 5442 6365 6909 7882 8676 8696 2234 Size: 692 172 923 544 973 794 20 3904 Fragments arranged by size: 3904 973 923 794 758 692 684 544 515 285 172 75 20 16 6 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10366 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3847 3882 529 Size: 35 3283 35 7013 Fragments arranged by size: 7013 3283 35 35 DraI TTT'AAA Cuts at: 7670 7670 Size: 10366 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3749 9373 809 Size: 507 2433 5624 1802 Fragments arranged by size: 5624 2433 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2183 2193 Size: 54 1425 50 275 18 249 104 10 Cuts at: 2193 2897 2971 3091 3261 3883 4452 4507 5151 Size: 704 74 120 170 622 569 55 644 Cuts at: 5151 5158 5737 5814 6186 6486 6537 6855 6919 Size: 7 579 77 372 300 51 318 64 Cuts at: 6919 7188 7813 8702 10331 8 Size: 269 625 889 1629 43 Fragments arranged by size: 1629 1425 889 704 644 625 622 579 569 372 318 300 275 269 249 170 120 104 77 74 64 55 54 51 50 43 18 10 7 EagI C'GGCC_G Cuts at: 1537 1812 2183 2897 3091 5737 6855 7188 7813 Size: 275 371 714 194 2646 1118 333 625 Cuts at: 7813 1537 Size: 4090 Fragments arranged by size: 4090 2646 1118 714 625 371 333 275 194 EarI CTCTTCn'nnn_ Cuts at: 1353 4698 7082 7406 7451 8296 1353 Size: 3345 2384 324 45 845 3423 Fragments arranged by size: 3423 3345 2384 845 324 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2059 2519 8953 9327 9473 387 Size: 1064 138 470 460 6434 374 146 1280 Fragments arranged by size: 6434 1280 1064 470 460 374 146 138 EcoICRI GAG'CTC Cuts at: 2386 2649 4669 4744 6531 2386 Size: 263 2020 75 1787 6221 Fragments arranged by size: 6221 2020 1787 263 75 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2414 2429 2434 2435 2843 3104 Size: 393 533 249 15 5 1 408 261 Cuts at: 3104 3442 3683 4393 5081 6261 6547 7003 7048 Size: 338 241 710 688 1180 286 456 45 Cuts at: 7048 7178 7294 8429 9026 9813 10081 1239 Size: 130 116 1135 597 787 268 1524 Fragments arranged by size: 1524 1180 1135 787 710 688 597 533 456 408 393 338 286 268 261 249 241 130 116 45 15 5 1 EcoNI CCTnn'n_nnAGG Cuts at: 4679 5483 6133 7632 4679 Size: 804 650 1499 7413 Fragments arranged by size: 7413 1499 804 650 EcoO109I rG'GnC_Cy Cuts at: 2063 2320 2425 2876 3435 3658 4004 5392 5476 Size: 257 105 451 559 223 346 1388 84 Cuts at: 5476 6178 6316 6317 6636 7182 7283 2063 Size: 702 138 1 319 546 101 5146 Fragments arranged by size: 5146 1388 702 559 546 451 346 319 257 223 138 105 101 84 1 EcoRI G'AATT_C Cuts at: 7675 8053 7675 Size: 378 9988 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5044 5044 Size: 10366 FspI TGC'GCA Cuts at: 8872 8872 Size: 10366 HaeII r_GCGC'y Cuts at: 1678 3417 5376 9513 1678 Size: 1739 1959 4137 2531 Fragments arranged by size: 4137 2531 1959 1739 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2498 2530 3935 3967 4002 Size: 32 135 32 926 32 1405 32 35 Cuts at: 4002 4034 5190 5222 5777 5809 6743 6775 6857 Size: 32 1156 32 555 32 934 32 82 Cuts at: 6857 6889 7160 7192 9007 9039 9081 9113 1373 Size: 32 271 32 1815 32 42 32 2626 Fragments arranged by size: 2626 1815 1405 1156 934 926 555 271 135 82 42 35 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2134 4963 7710 7956 669 Size: 988 281 196 2829 2747 246 3079 Fragments arranged by size: 3079 2829 2747 988 281 246 196 HindIII A'AGCT_T Cuts at: 748 748 Size: 10366 HpaI GTT'AAC Cuts at: 2134 7956 2134 Size: 5822 4544 KasI G'GCGC_C Cuts at: 3413 3413 Size: 10366 KpnI G_GTAC'C Cuts at: 7691 7691 Size: 10366 MfeI C'AATT_G Cuts at: 7965 7965 Size: 10366 MluI A'CGCG_T Cuts at: 10242 10242 Size: 10366 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4911 5352 5722 9480 9664 1305 Size: 3606 441 370 3758 184 2007 Fragments arranged by size: 3758 3606 2007 441 370 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2195 2973 4454 4509 5153 Size: 54 1425 343 363 778 1481 55 644 Cuts at: 5153 5816 6488 10 Size: 663 672 3888 Fragments arranged by size: 3888 1481 1425 778 672 663 644 363 343 55 54 MslI CAynn'nnrTG Cuts at: 518 1166 2624 2927 3066 3284 3394 3579 3897 Size: 648 1458 303 139 218 110 185 318 Cuts at: 3897 4676 4871 4955 5537 5555 5729 6006 6059 Size: 779 195 84 582 18 174 277 53 Cuts at: 6059 6464 7343 8324 8683 8842 518 Size: 405 879 981 359 159 2042 Fragments arranged by size: 2042 1458 981 879 779 648 582 405 359 318 303 277 218 195 185 174 159 139 110 84 53 18 MspA1I CmG'CkG Cuts at: 1606 2075 2169 2209 2947 3462 3822 4055 4291 Size: 469 94 40 738 515 360 233 236 Cuts at: 4291 4309 4483 5145 5803 5866 6834 6967 7268 Size: 18 174 662 658 63 968 133 301 Cuts at: 7268 7469 7782 8459 9607 9852 1606 Size: 201 313 677 1148 245 2120 Fragments arranged by size: 2120 1148 968 738 677 662 658 515 469 360 313 301 245 236 233 201 174 133 94 63 40 18 NaeI GCC'GGC Cuts at: 6855 6855 Size: 10366 NarI GG'CG_CC Cuts at: 3414 3414 Size: 10366 NcoI C'CATG_G Cuts at: 513 1065 2004 4836 6459 513 Size: 552 939 2832 1623 4420 Fragments arranged by size: 4420 2832 1623 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10366 NgoMIV G'CCGG_C Cuts at: 6853 6853 Size: 10366 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10366 NotI GC'GGCC_GC Cuts at: 7813 7813 Size: 10366 NsiI A_TGCA'T Cuts at: 7603 7603 Size: 10366 NspI r_CATG'y Cuts at: 1132 1229 2992 4300 6005 7724 9269 1132 Size: 97 1763 1308 1705 1719 1545 2229 Fragments arranged by size: 2229 1763 1719 1705 1545 1308 97 PasI CC'CwG_GG Cuts at: 1348 3788 3847 3970 1348 Size: 2440 59 123 7744 Fragments arranged by size: 7744 2440 123 59 PciI A'CATG_T Cuts at: 4296 9265 4296 Size: 4969 5397 PflMI CCAn_nnn'nTGG Cuts at: 1108 2428 3616 3661 4014 1108 Size: 1320 1188 45 353 7460 Fragments arranged by size: 7460 1320 1188 353 45 PfoI T'CCnGG_A Cuts at: 2670 4103 5661 5970 6711 7413 2670 Size: 1433 1558 309 741 702 5623 Fragments arranged by size: 5623 1558 1433 741 702 309 PmeI GTTT'AAAC Cuts at: 7670 7670 Size: 10366 PmlI CAC'GTG Cuts at: 3647 3647 Size: 10366 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5585 5617 8435 8467 9948 9980 5585 Size: 32 2818 32 1481 32 5971 Fragments arranged by size: 5971 2818 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2320 4004 5476 6636 7182 2320 Size: 1684 1472 1160 546 5504 Fragments arranged by size: 5504 1684 1472 1160 546 PshAI GACnn'nnGTC Cuts at: 1613 5704 7469 1613 Size: 4091 1765 4510 Fragments arranged by size: 4510 4091 1765 PsiI TTA'TAA Cuts at: 7936 7936 Size: 10366 PspOMI G'GGCC_C Cuts at: 2062 2875 3435 6316 7376 2062 Size: 813 560 2881 1060 5052 Fragments arranged by size: 5052 2881 1060 813 560 PspXI vC'TCGA_Gb Cuts at: 4022 7539 4022 Size: 3517 6849 PstI C_TGCA'G Cuts at: 830 2704 2946 3821 4317 5865 5931 7528 7718 Size: 1874 242 875 496 1548 66 1597 190 Cuts at: 7718 830 Size: 3478 Fragments arranged by size: 3478 1874 1597 1548 875 496 242 190 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10366 PvuII CAG'CTG Cuts at: 2947 3462 4291 4483 5803 5866 6834 2947 Size: 515 829 192 1320 63 968 6479 Fragments arranged by size: 6479 1320 968 829 515 192 63 RsrII CG'GwC_CG Cuts at: 5754 5754 Size: 10366 SacI G_AGCT'C Cuts at: 2388 2651 4671 4746 6533 2388 Size: 263 2020 75 1787 6221 Fragments arranged by size: 6221 2020 1787 263 75 SacII CC_GC'GG Cuts at: 5146 7269 5146 Size: 2123 8243 SalI G'TCGA_C Cuts at: 1936 7708 1936 Size: 5772 4594 SanDI GG'GwC_CC Cuts at: 7182 7182 Size: 10366 SapI GCTCTTCn'nnn_ Cuts at: 7451 7451 Size: 10366 SbfI CC_TGCA'GG Cuts at: 7718 7718 Size: 10366 ScaI AGT'ACT Cuts at: 6926 8614 10326 6926 Size: 1688 1712 6966 Fragments arranged by size: 6966 1712 1688 SexAI A'CCwGG_T Cuts at: 3480 7425 3480 Size: 3945 6421 SfcI C'TryA_G Cuts at: 826 1045 2700 2942 3317 3817 3874 4313 5861 Size: 219 1655 242 375 500 57 439 1548 Cuts at: 5861 5927 6428 6720 7524 7714 8849 9530 9721 Size: 66 501 292 804 190 1135 681 191 Cuts at: 9721 826 Size: 1471 Fragments arranged by size: 1655 1548 1471 1135 804 681 501 500 439 375 292 242 219 191 190 66 57 SfiI GGCCn_nnn'nGGCC Cuts at: 6185 6185 Size: 10366 SfoI GGC'GCC Cuts at: 3415 3415 Size: 10366 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10366 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10366 SmaI CCC'GGG Cuts at: 1872 2874 4857 6396 6587 7508 7693 1872 Size: 1002 1983 1539 191 921 185 4545 Fragments arranged by size: 4545 1983 1539 1002 921 191 185 SmlI C'TyrA_G Cuts at: 820 1017 1943 2657 3721 3932 4022 6876 7539 Size: 197 926 714 1064 211 90 2854 663 Cuts at: 7539 8470 9371 9633 820 Size: 931 901 262 1553 Fragments arranged by size: 2854 1553 1064 931 926 901 714 663 262 211 197 90 SnaBI TAC'GTA Cuts at: 493 2562 8157 493 Size: 2069 5595 2702 Fragments arranged by size: 5595 2702 2069 SpeI A'CTAG_T Cuts at: 152 152 Size: 10366 SphI G_CATG'C Cuts at: 1132 2992 7724 1132 Size: 1860 4732 3774 Fragments arranged by size: 4732 3774 1860 SrfI GCCC'GGGC Cuts at: 6587 6587 Size: 10366 SspI AAT'ATT Cuts at: 5 52 5898 8290 5 Size: 47 5846 2392 2081 Fragments arranged by size: 5846 2392 2081 47 StuI AGG'CCT Cuts at: 1517 2150 4145 5625 6513 6809 1517 Size: 633 1995 1480 888 296 5074 Fragments arranged by size: 5074 1995 1480 888 633 296 StyI C'CwwG_G Cuts at: 513 1065 2004 2968 3046 3960 4013 4146 4836 Size: 552 939 964 78 914 53 133 690 Cuts at: 4836 4911 6459 7279 7805 513 Size: 75 1548 820 526 3074 Fragments arranged by size: 3074 1548 964 939 914 820 690 552 526 133 78 75 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3067 4139 5567 6179 6256 6803 6831 Size: 1255 1487 1072 1428 612 77 547 28 Cuts at: 6831 7212 7600 8393 8410 8563 8748 9193 325 Size: 381 388 793 17 153 185 445 1498 Fragments arranged by size: 1498 1487 1428 1255 1072 793 612 547 445 388 381 185 153 77 28 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3423 4947 5712 Size: 274 80 33 51 1443 1446 1524 765 Cuts at: 5712 6924 8612 10324 96 Size: 1212 1688 1712 138 Fragments arranged by size: 1712 1688 1524 1446 1443 1212 765 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3358 3390 6192 6224 10326 10358 3358 Size: 32 2802 32 4102 32 3366 Fragments arranged by size: 4102 3366 2802 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2041 1316 Size: 725 9641 XbaI T'CTAG_A Cuts at: 7702 7702 Size: 10366 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3446 4288 4447 6236 6571 7042 3446 Size: 842 159 1789 335 471 6770 Fragments arranged by size: 6770 1789 842 471 335 159 XhoI C'TCGA_G Cuts at: 1943 4022 7539 1943 Size: 2079 3517 4770 Fragments arranged by size: 4770 3517 2079 XmaI C'CCGG_G Cuts at: 1870 2872 4855 6394 6585 7506 7691 1870 Size: 1002 1983 1539 191 921 185 4545 Fragments arranged by size: 4545 1983 1539 1002 921 191 185 XmnI GAAnn'nnTTC Cuts at: 2447 5027 5235 5597 5988 8495 2447 Size: 2580 208 362 391 2507 4318 Fragments arranged by size: 4318 2580 2507 391 362 208 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2755 5659 8174 276 Size: 53 83 186 936 1221 2904 2515 2468 Fragments arranged by size: 2904 2515 2468 1221 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI PacI PsrI SwaI