(Circular) (Six-base) MAPSORT of: pFN21AA0850.seq Check: 8781 from: 1 to: 6631 pFN21AA0850 6631 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3113 3113 Size: 6631 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4441 278 Size: 53 83 186 936 2905 2468 Fragments arranged by size: 2905 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3974 1937 Size: 2037 4594 Acc65I G'GTAC_C Cuts at: 3952 3952 Size: 6631 AclI AA'CG_TT Cuts at: 1563 4758 5131 1563 Size: 3195 373 3063 Fragments arranged by size: 3195 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2360 2520 2868 4694 6078 6346 1632 Size: 464 264 160 348 1826 1384 268 1917 Fragments arranged by size: 1917 1826 1384 464 348 268 264 160 AflII C'TTAA_G Cuts at: 820 1017 2516 820 Size: 197 1499 4935 Fragments arranged by size: 4935 1499 197 AflIII A'CryG_T Cuts at: 1203 5530 6507 1203 Size: 4327 977 1327 Fragments arranged by size: 4327 1327 977 AgeI A'CCGG_T Cuts at: 5462 5462 Size: 6631 AhdI GACnn_n'nnGTC Cuts at: 2114 5360 2114 Size: 3246 3385 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6631 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6631 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3654 3686 3654 Size: 32 6599 AlwNI CAG_nnn'CTG Cuts at: 2249 4374 5946 2249 Size: 2125 1572 2934 Fragments arranged by size: 2934 2125 1572 ApaLI G'TGCA_C Cuts at: 4688 5844 4688 Size: 1156 5475 ApoI r'AATT_y Cuts at: 1493 2041 2211 2225 2419 2493 3857 3940 4166 Size: 548 170 14 194 74 1364 83 226 Cuts at: 4166 4318 6427 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1364 548 226 194 170 152 83 74 14 AseI AT'TA_AT Cuts at: 160 1032 5185 160 Size: 872 4153 1606 Fragments arranged by size: 4153 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6631 AvaI C'yCGr_G Cuts at: 1870 1943 3956 1870 Size: 73 2013 4545 Fragments arranged by size: 4545 2013 73 BamHI G'GATC_C Cuts at: 3961 4328 3961 Size: 367 6264 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3952 5407 618 Size: 325 217 627 2165 1455 1842 Fragments arranged by size: 2165 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2981 3223 3908 3950 1391 Size: 1590 242 685 42 4072 Fragments arranged by size: 4072 1590 685 242 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2046 3140 928 Size: 971 147 1094 4419 Fragments arranged by size: 4419 1094 971 147 BbvCI CC'TCA_GC Cuts at: 2727 2727 Size: 6631 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4822 4856 1903 Size: 34 2885 34 3678 Fragments arranged by size: 3678 2885 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4523 5733 4523 Size: 1210 5421 BclI T'GATC_A Cuts at: 1548 2686 1548 Size: 1138 5493 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5242 136 Size: 107 122 71 631 605 3570 1525 Fragments arranged by size: 3570 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6626 6626 Size: 6631 BlpI GC'TnA_GC Cuts at: 4048 4048 Size: 6631 Bme1580I G_kGCm'C Cuts at: 1790 3493 4692 5848 1790 Size: 1703 1199 1156 2573 Fragments arranged by size: 2573 1703 1199 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6631 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2614 4362 5320 Size: 416 53 83 399 24 1197 1748 958 Cuts at: 5320 442 Size: 1753 Fragments arranged by size: 1753 1748 1197 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2156 1056 Size: 1100 5531 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3681 5291 1239 Size: 2442 1610 2579 Fragments arranged by size: 2579 2442 1610 Bpu10I CC'TnA_GC Cuts at: 2727 2859 2727 Size: 132 6499 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3908 4756 5621 5919 3908 Size: 848 865 298 4620 Fragments arranged by size: 4620 865 848 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5294 882 Size: 617 3795 2219 Fragments arranged by size: 3795 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2783 4422 493 Size: 711 1579 1639 2702 Fragments arranged by size: 2702 1639 1579 711 BsaBI GATnn'nnATC Cuts at: 2199 2238 6625 2199 Size: 39 4387 2205 Fragments arranged by size: 4387 2205 39 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4438 4820 5455 275 Size: 53 83 186 936 2905 382 635 1451 Fragments arranged by size: 2905 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5064 5462 5736 5883 6294 5064 Size: 398 274 147 411 5401 Fragments arranged by size: 5401 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2471 2501 2977 3007 3648 Size: 30 165 30 1068 30 476 30 641 Cuts at: 3648 3654 3678 3684 1178 Size: 6 24 6 4125 Fragments arranged by size: 4125 1068 641 476 165 30 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2519 2522 3669 1186 Size: 1333 3 1147 4148 Fragments arranged by size: 4148 1333 1147 3 BseYI C'CCAG_C Cuts at: 2570 3800 5834 6330 2570 Size: 1230 2034 496 2871 Fragments arranged by size: 2871 2034 1230 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2630 2786 3582 1163 Size: 573 894 156 796 4212 Fragments arranged by size: 4212 894 796 573 156 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4081 4842 5870 1540 Size: 275 186 2080 761 1028 2301 Fragments arranged by size: 2301 2080 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2981 3385 4692 4777 5848 2981 Size: 404 1307 85 1071 3764 Fragments arranged by size: 3764 1307 1071 404 85 BsmI GAATG_Cn' Cuts at: 3167 4142 4235 3167 Size: 975 93 5563 Fragments arranged by size: 5563 975 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2981 3223 3385 3493 3908 3950 4692 Size: 399 1191 242 162 108 415 42 742 Cuts at: 4692 4777 5848 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1191 1071 742 415 399 242 162 108 85 42 BspEI T'CCGG_A Cuts at: 6294 6294 Size: 6631 BspHI T'CATG_A Cuts at: 4520 6250 4520 Size: 1730 4901 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2350 3113 3986 844 Size: 1506 763 873 3489 Fragments arranged by size: 3489 1506 873 763 BsrBI CCG'CTC Cuts at: 1394 1823 4518 1394 Size: 429 2695 3507 Fragments arranged by size: 3507 2695 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2581 3802 3859 5126 5300 65 Size: 1183 1333 1221 57 1267 174 1396 Fragments arranged by size: 1396 1333 1267 1221 1183 174 57 BsrFI r'CCGG_y Cuts at: 1700 1810 5275 5462 1700 Size: 110 3465 187 2869 Fragments arranged by size: 3465 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3546 96 Size: 3450 3181 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6631 BssSI C'ACGA_G Cuts at: 4691 5703 4691 Size: 1012 5619 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6631 BstBI TT'CG_AA Cuts at: 3151 5435 3151 Size: 2284 4347 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6631 BstXI CCAn_nnnn'nTGG Cuts at: 3643 3643 Size: 6631 BstYI r'GATC_y Cuts at: 1972 3961 4328 4713 4730 6182 6268 6280 6626 Size: 1989 367 385 17 1452 86 12 346 Cuts at: 6626 1972 Size: 1977 Fragments arranged by size: 1989 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2078 2078 Size: 6631 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6079 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5892 Fragments arranged by size: 5892 683 56 BtsI GCAGTG_nn' Cuts at: 2154 2277 2746 3840 4147 4941 4961 2154 Size: 123 469 1094 307 794 20 3824 Fragments arranged by size: 3824 1094 794 469 307 123 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6631 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6596 DraI TTT'AAA Cuts at: 3935 3935 Size: 6631 DraIII CAC_nnn'GTG Cuts at: 2783 2783 Size: 6631 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5638 809 Size: 507 4322 1802 Fragments arranged by size: 4322 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2089 2122 2791 Size: 54 1425 50 275 18 259 33 669 Cuts at: 2791 3277 4078 4967 6596 8 Size: 486 801 889 1629 43 Fragments arranged by size: 1629 1425 889 801 669 486 275 259 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 4078 1537 Size: 275 2266 4090 Fragments arranged by size: 4090 2266 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2496 2640 2652 2836 4561 1353 Size: 1143 144 12 184 1725 3423 Fragments arranged by size: 3423 1725 1143 184 144 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5218 5592 5738 387 Size: 1064 138 3629 374 146 1280 Fragments arranged by size: 3629 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2979 2979 Size: 6631 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2360 2520 2868 3681 4694 5291 Size: 393 464 264 160 348 813 1013 597 Cuts at: 5291 6078 6346 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1013 813 787 597 464 393 348 268 264 160 EcoRI G'AATT_C Cuts at: 3940 4318 3940 Size: 378 6253 FspI TGC'GCA Cuts at: 5137 5137 Size: 6631 HaeII r_GCGC'y Cuts at: 1678 5778 1678 Size: 4100 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2100 2132 3173 3205 5272 Size: 32 135 32 528 32 1041 32 2067 Cuts at: 5272 5304 5346 5378 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2067 1041 528 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3975 4221 669 Size: 988 281 2037 246 3079 Fragments arranged by size: 3079 2037 988 281 246 HindIII A'AGCT_T Cuts at: 748 2519 3016 748 Size: 1771 497 4363 Fragments arranged by size: 4363 1771 497 HpaI GTT'AAC Cuts at: 4221 4221 Size: 6631 KpnI G_GTAC'C Cuts at: 3956 3956 Size: 6631 MfeI C'AATT_G Cuts at: 4230 4230 Size: 6631 MluI A'CGCG_T Cuts at: 6507 6507 Size: 6631 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2517 3770 5745 5929 1305 Size: 1212 1253 1975 184 2007 Fragments arranged by size: 2007 1975 1253 1212 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2091 2124 2793 3279 10 Size: 54 1425 343 259 33 669 486 3362 Fragments arranged by size: 3362 1425 669 486 343 259 54 33 MslI CAynn'nnrTG Cuts at: 518 1166 3285 3641 4589 4948 5107 518 Size: 648 2119 356 948 359 159 2042 Fragments arranged by size: 2119 2042 948 648 359 356 159 MspA1I CmG'CkG Cuts at: 1606 2403 2684 4047 4724 5872 6117 1606 Size: 797 281 1363 677 1148 245 2120 Fragments arranged by size: 2120 1363 1148 797 677 281 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6079 NdeI CA'TA_TG Cuts at: 387 3424 387 Size: 3037 3594 NheI G'CTAG_C Cuts at: 1052 2152 1052 Size: 1100 5531 NotI GC'GGCC_GC Cuts at: 4078 4078 Size: 6631 NspI r_CATG'y Cuts at: 1132 1229 3989 5534 1132 Size: 97 2760 1545 2229 Fragments arranged by size: 2760 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6631 PciI A'CATG_T Cuts at: 5530 5530 Size: 6631 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6631 PmeI GTTT'AAAC Cuts at: 3935 3935 Size: 6631 PmlI CAC'GTG Cuts at: 2783 2783 Size: 6631 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3654 3686 4700 4732 6213 6245 3654 Size: 32 1014 32 1481 32 4040 Fragments arranged by size: 4040 1481 1014 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3514 1613 Size: 1901 4730 PsiI TTA'TAA Cuts at: 4201 4201 Size: 6631 PstI C_TGCA'G Cuts at: 830 2165 2329 3124 3523 3983 830 Size: 1335 164 795 399 460 3478 Fragments arranged by size: 3478 1335 795 460 399 164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6631 PvuII CAG'CTG Cuts at: 2403 2684 2403 Size: 281 6350 SacI G_AGCT'C Cuts at: 2981 2981 Size: 6631 SalI G'TCGA_C Cuts at: 1936 3973 1936 Size: 2037 4594 SapI GCTCTTCn'nnn_ Cuts at: 2640 2640 Size: 6631 SbfI CC_TGCA'GG Cuts at: 3983 3983 Size: 6631 ScaI AGT'ACT Cuts at: 2992 4879 6591 2992 Size: 1887 1712 3032 Fragments arranged by size: 3032 1887 1712 SfcI C'TryA_G Cuts at: 826 1045 2161 2325 2844 3120 3519 3813 3979 Size: 219 1116 164 519 276 399 294 166 Cuts at: 3979 5114 5795 5986 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1116 681 519 399 294 276 219 191 166 164 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6631 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6631 SmaI CCC'GGG Cuts at: 1872 3958 1872 Size: 2086 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2516 3887 4735 5636 5898 820 Size: 197 926 573 1371 848 901 262 1553 Fragments arranged by size: 1553 1371 926 901 848 573 262 197 SnaBI TAC'GTA Cuts at: 493 4422 493 Size: 3929 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6631 SphI G_CATG'C Cuts at: 1132 3989 1132 Size: 2857 3774 SspI AAT'ATT Cuts at: 5 52 4555 5 Size: 47 4503 2081 Fragments arranged by size: 4503 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6631 StyI C'CwwG_G Cuts at: 513 1065 2525 3788 4070 513 Size: 552 1460 1263 282 3074 Fragments arranged by size: 3074 1460 1263 552 282 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2596 3649 4658 4675 4828 5013 5458 Size: 1255 1016 1053 1009 17 153 185 445 Cuts at: 5458 325 Size: 1498 Fragments arranged by size: 1498 1255 1053 1016 1009 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2673 2848 2990 Size: 274 80 33 51 1443 696 175 142 Cuts at: 2990 3238 3546 4877 6589 96 Size: 248 308 1331 1712 138 Fragments arranged by size: 1712 1443 1331 696 308 274 248 175 142 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3472 3504 3648 3680 6591 6623 3472 Size: 32 144 32 2911 32 3480 Fragments arranged by size: 3480 2911 144 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6631 XbaI T'CTAG_A Cuts at: 2384 3967 2384 Size: 1583 5048 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3727 3832 3727 Size: 105 6526 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6631 XmaI C'CCGG_G Cuts at: 1870 3956 1870 Size: 2086 4545 XmnI GAAnn'nnTTC Cuts at: 2661 3147 4760 2661 Size: 486 1613 4532 Fragments arranged by size: 4532 1613 486 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4439 276 Size: 53 83 186 936 2905 2468 Fragments arranged by size: 2905 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI