(Circular) (Six-base) MAPSORT of: pFN21AA0744.seq Check: 8991 from: 1 to: 6475 pFN21AA0744 6475 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4285 278 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3818 1937 Size: 1881 4594 Acc65I G'GTAC_C Cuts at: 3311 3796 3311 Size: 485 5990 AclI AA'CG_TT Cuts at: 1563 4602 4975 1563 Size: 3039 373 3063 Fragments arranged by size: 3063 3039 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2237 3509 3515 4538 5922 6190 1632 Size: 605 1272 6 1023 1384 268 1917 Fragments arranged by size: 1917 1384 1272 1023 605 268 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6278 AflIII A'CryG_T Cuts at: 1203 5374 6351 1203 Size: 4171 977 1327 Fragments arranged by size: 4171 1327 977 AgeI A'CCGG_T Cuts at: 5306 5306 Size: 6475 AhdI GACnn_n'nnGTC Cuts at: 5204 5204 Size: 6475 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6475 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6475 AlwNI CAG_nnn'CTG Cuts at: 2194 2320 2822 4218 5790 2194 Size: 126 502 1396 1572 2879 Fragments arranged by size: 2879 1572 1396 502 126 ApaLI G'TGCA_C Cuts at: 4532 5688 4532 Size: 1156 5319 ApoI r'AATT_y Cuts at: 1493 3037 3291 3784 4010 4162 6271 1493 Size: 1544 254 493 226 152 2109 1697 Fragments arranged by size: 2109 1697 1544 493 254 226 152 AseI AT'TA_AT Cuts at: 160 1032 5029 160 Size: 872 3997 1606 Fragments arranged by size: 3997 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 AvaI C'yCGr_G Cuts at: 1870 1943 3800 1870 Size: 73 1857 4545 Fragments arranged by size: 4545 1857 73 BamHI G'GATC_C Cuts at: 3805 4172 3805 Size: 367 6108 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3311 3796 5251 618 Size: 325 217 627 1524 485 1455 1842 Fragments arranged by size: 1842 1524 1455 627 485 325 217 BanII G_rGCy'C Cuts at: 1391 2064 2670 3794 1391 Size: 673 606 1124 4072 Fragments arranged by size: 4072 1124 673 606 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3561 928 Size: 971 1662 3842 Fragments arranged by size: 3842 1662 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3581 3615 4666 4700 1903 Size: 34 50 34 1560 34 1051 34 3678 Fragments arranged by size: 3678 1560 1051 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4367 5577 4367 Size: 1210 5265 BclI T'GATC_A Cuts at: 1548 2016 1548 Size: 468 6007 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5086 136 Size: 107 122 71 631 605 3414 1525 Fragments arranged by size: 3414 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6470 6470 Size: 6475 BlpI GC'TnA_GC Cuts at: 2247 3384 3892 2247 Size: 1137 508 4830 Fragments arranged by size: 4830 1137 508 Bme1580I G_kGCm'C Cuts at: 1790 4536 5692 1790 Size: 2746 1156 2573 Fragments arranged by size: 2746 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6475 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2823 3346 3629 Size: 416 53 83 399 24 1406 523 283 Cuts at: 3629 4206 5164 442 Size: 577 958 1753 Fragments arranged by size: 1753 1406 958 577 523 416 399 283 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6475 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2083 2083 Size: 6475 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2342 2799 3732 5135 1239 Size: 839 264 457 933 1403 2579 Fragments arranged by size: 2579 1403 933 839 457 264 Bpu10I CC'TnA_GC Cuts at: 3077 3077 Size: 6475 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3530 4600 5465 5763 3530 Size: 1070 865 298 4242 Fragments arranged by size: 4242 1070 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5138 882 Size: 617 3639 2219 Fragments arranged by size: 3639 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4266 493 Size: 711 3062 2702 Fragments arranged by size: 3062 2702 711 BsaBI GATnn'nnATC Cuts at: 6469 6469 Size: 6475 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4282 4664 5299 275 Size: 53 83 186 936 2749 382 635 1451 Fragments arranged by size: 2749 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4908 5306 5580 5727 6138 4908 Size: 398 274 147 411 5245 Fragments arranged by size: 5245 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2283 2313 1178 Size: 30 165 30 880 30 5340 Fragments arranged by size: 5340 880 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2370 2728 1186 Size: 1184 358 4933 Fragments arranged by size: 4933 1184 358 BseYI C'CCAG_C Cuts at: 3021 5678 6174 3021 Size: 2657 496 3322 Fragments arranged by size: 3322 2657 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3342 1163 Size: 573 1606 4296 Fragments arranged by size: 4296 1606 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3925 4686 5714 1540 Size: 275 186 1924 761 1028 2301 Fragments arranged by size: 2301 1924 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2623 3377 4536 4621 5692 2623 Size: 754 1159 85 1071 3406 Fragments arranged by size: 3406 1159 1071 754 85 BsmI GAATG_Cn' Cuts at: 2928 3986 4079 2928 Size: 1058 93 5324 Fragments arranged by size: 5324 1058 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3059 3059 Size: 6475 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2623 2670 3377 3794 4536 4621 Size: 399 274 559 47 707 417 742 85 Cuts at: 4621 5692 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 707 559 417 399 274 85 47 BspEI T'CCGG_A Cuts at: 6138 6138 Size: 6475 BspHI T'CATG_A Cuts at: 2938 4364 6094 2938 Size: 1426 1730 3319 Fragments arranged by size: 3319 1730 1426 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2964 3830 844 Size: 2120 866 3489 Fragments arranged by size: 3489 2120 866 BsrBI CCG'CTC Cuts at: 1394 1823 4362 1394 Size: 429 2539 3507 Fragments arranged by size: 3507 2539 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4970 5144 65 Size: 1183 3722 174 1396 Fragments arranged by size: 3722 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5119 5306 1700 Size: 110 3309 187 2869 Fragments arranged by size: 3309 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6475 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6475 BssSI C'ACGA_G Cuts at: 4535 5547 4535 Size: 1012 5463 BstAPI GCAn_nnn'nTGC Cuts at: 794 2627 794 Size: 1833 4642 BstBI TT'CG_AA Cuts at: 2650 3035 3470 5279 2650 Size: 385 435 1809 3846 Fragments arranged by size: 3846 1809 435 385 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6475 BstYI r'GATC_y Cuts at: 1972 3447 3681 3805 4172 4557 4574 6026 6112 Size: 1475 234 124 367 385 17 1452 86 Cuts at: 6112 6124 6470 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1475 1452 385 367 346 234 124 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2081 2093 2081 Size: 12 6463 BtgI C'CryG_G Cuts at: 513 1065 2109 513 Size: 552 1044 4879 Fragments arranged by size: 4879 1044 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2342 3560 487 Size: 683 56 1116 1218 3402 Fragments arranged by size: 3402 1218 1116 683 56 BtsI GCAGTG_nn' Cuts at: 2421 3018 3604 3991 4785 4805 2421 Size: 597 586 387 794 20 4091 Fragments arranged by size: 4091 794 597 586 387 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6475 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6440 DraI TTT'AAA Cuts at: 3779 3779 Size: 6475 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2123 5482 809 Size: 507 807 3359 1802 Fragments arranged by size: 3359 1802 807 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3922 4811 6440 Size: 54 1425 50 275 18 2092 889 1629 Cuts at: 6440 8 Size: 43 Fragments arranged by size: 2092 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3922 1537 Size: 275 2110 4090 Fragments arranged by size: 4090 2110 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3519 4405 1353 Size: 2166 886 3423 Fragments arranged by size: 3423 2166 886 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2752 5062 5436 5582 387 Size: 1064 138 1163 2310 374 146 1280 Fragments arranged by size: 2310 1280 1163 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2237 2342 2799 3509 3515 3732 Size: 393 446 159 105 457 710 6 217 Cuts at: 3732 4538 5135 5922 6190 1239 Size: 806 597 787 268 1524 Fragments arranged by size: 1524 806 787 710 597 457 446 393 268 217 159 105 6 EcoNI CCTnn'n_nnAGG Cuts at: 2306 2385 2731 2306 Size: 79 346 6050 Fragments arranged by size: 6050 346 79 EcoRI G'AATT_C Cuts at: 3037 3784 4162 3037 Size: 747 378 5350 Fragments arranged by size: 5350 747 378 FspI TGC'GCA Cuts at: 4981 4981 Size: 6475 HaeII r_GCGC'y Cuts at: 1678 3566 5622 1678 Size: 1888 2056 2531 Fragments arranged by size: 2531 2056 1888 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 2503 2535 2552 Size: 32 135 32 449 32 450 32 17 Cuts at: 2552 2584 3102 3134 5116 5148 5190 5222 1373 Size: 32 518 32 1982 32 42 32 2626 Fragments arranged by size: 2626 1982 518 450 449 135 42 32 32 32 32 32 32 32 32 17 HincII GTy'rAC Cuts at: 669 1657 1938 3819 4065 669 Size: 988 281 1881 246 3079 Fragments arranged by size: 3079 1881 988 281 246 HindIII A'AGCT_T Cuts at: 748 2441 3230 748 Size: 1693 789 3993 Fragments arranged by size: 3993 1693 789 HpaI GTT'AAC Cuts at: 4065 4065 Size: 6475 KpnI G_GTAC'C Cuts at: 3315 3800 3315 Size: 485 5990 MfeI C'AATT_G Cuts at: 2138 4074 2138 Size: 1936 4539 MluI A'CGCG_T Cuts at: 6351 6351 Size: 6475 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2510 5589 5773 1305 Size: 1205 3079 184 2007 Fragments arranged by size: 3079 2007 1205 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4653 Fragments arranged by size: 4653 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3141 4433 4792 4951 518 Size: 648 1975 1292 359 159 2042 Fragments arranged by size: 2042 1975 1292 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3388 3891 4568 5716 5961 1606 Size: 1782 503 677 1148 245 2120 Fragments arranged by size: 2120 1782 1148 677 503 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5923 NdeI CA'TA_TG Cuts at: 387 387 Size: 6475 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6475 NotI GC'GGCC_GC Cuts at: 3922 3922 Size: 6475 NspI r_CATG'y Cuts at: 1132 1229 3833 5378 1132 Size: 97 2604 1545 2229 Fragments arranged by size: 2604 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6475 PciI A'CATG_T Cuts at: 5374 5374 Size: 6475 PflMI CCAn_nnn'nTGG Cuts at: 1108 2492 2529 2822 2843 3691 1108 Size: 1384 37 293 21 848 3892 Fragments arranged by size: 3892 1384 848 293 37 21 PfoI T'CCnGG_A Cuts at: 3684 3748 3684 Size: 64 6411 PmeI GTTT'AAAC Cuts at: 3779 3779 Size: 6475 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4544 4576 6057 6089 4544 Size: 32 1481 32 4930 Fragments arranged by size: 4930 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6475 PsiI TTA'TAA Cuts at: 4045 4045 Size: 6475 PstI C_TGCA'G Cuts at: 830 3195 3827 830 Size: 2365 632 3478 Fragments arranged by size: 3478 2365 632 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6475 PvuII CAG'CTG Cuts at: 3388 3388 Size: 6475 SalI G'TCGA_C Cuts at: 1936 3817 1936 Size: 1881 4594 SapI GCTCTTCn'nnn_ Cuts at: 3519 3519 Size: 6475 SbfI CC_TGCA'GG Cuts at: 3827 3827 Size: 6475 ScaI AGT'ACT Cuts at: 4723 6435 4723 Size: 1712 4763 SfcI C'TryA_G Cuts at: 826 1045 3191 3823 4958 5639 5830 826 Size: 219 2146 632 1135 681 191 1471 Fragments arranged by size: 2146 1471 1135 681 632 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6475 SmaI CCC'GGG Cuts at: 1872 3802 1872 Size: 1930 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3509 4579 5480 5742 820 Size: 197 926 1566 1070 901 262 1553 Fragments arranged by size: 1566 1553 1070 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4266 493 Size: 3773 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6475 SphI G_CATG'C Cuts at: 1132 3833 1132 Size: 2701 3774 SspI AAT'ATT Cuts at: 5 52 4399 5 Size: 47 4347 2081 Fragments arranged by size: 4347 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6475 StyI C'CwwG_G Cuts at: 513 1065 2997 3273 3914 513 Size: 552 1932 276 641 3074 Fragments arranged by size: 3074 1932 641 552 276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3181 4502 4519 4672 4857 5302 325 Size: 1255 1601 1321 17 153 185 445 1498 Fragments arranged by size: 1601 1498 1321 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2423 2604 4721 Size: 274 80 33 51 1443 446 181 2117 Cuts at: 4721 6433 96 Size: 1712 138 Fragments arranged by size: 2117 1712 1443 446 274 181 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6435 6467 6435 Size: 32 6443 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6475 XbaI T'CTAG_A Cuts at: 3811 3811 Size: 6475 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2995 2995 Size: 6475 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6475 XmaI C'CCGG_G Cuts at: 1870 3800 1870 Size: 1930 4545 XmnI GAAnn'nnTTC Cuts at: 2154 3528 4604 2154 Size: 1374 1076 4025 Fragments arranged by size: 4025 1374 1076 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4283 276 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI