Restriction map for pFN21AA0744
 (Circular) (Six-base) MAPSORT of: pFN21AA0744.seq  Check: 8991  from: 1  to: 6475



pFN21AA0744  6475 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:23  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4285     278 
   Size:         53      83     186     936    2749    2468

  Fragments arranged by size: 

                2749    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3818    1937 
   Size:       1881    4594
 
Acc65I G'GTAC_C

Cuts at:   3311    3796    3311 
   Size:        485    5990
 
AclI AA'CG_TT

Cuts at:   1563    4602    4975    1563 
   Size:       3039     373    3063

  Fragments arranged by size: 

                3063    3039     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2237    3509    3515    4538    5922    6190    1632 
   Size:        605    1272       6    1023    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1272    1023     605     268       6
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6278
 
AflIII A'CryG_T

Cuts at:   1203    5374    6351    1203 
   Size:       4171     977    1327

  Fragments arranged by size: 

                4171    1327     977
 
AgeI A'CCGG_T

Cuts at:   5306    5306 
   Size:       6475
 
AhdI GACnn_n'nnGTC

Cuts at:   5204    5204 
   Size:       6475
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6475
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6475
 
AlwNI CAG_nnn'CTG

Cuts at:   2194    2320    2822    4218    5790    2194 
   Size:        126     502    1396    1572    2879

  Fragments arranged by size: 

                2879    1572    1396     502     126
 
ApaLI G'TGCA_C

Cuts at:   4532    5688    4532 
   Size:       1156    5319
 
ApoI r'AATT_y

Cuts at:   1493    3037    3291    3784    4010    4162    6271    1493 
   Size:       1544     254     493     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1544     493     254     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    5029     160 
   Size:        872    3997    1606

  Fragments arranged by size: 

                3997    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6475
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3800    1870 
   Size:         73    1857    4545

  Fragments arranged by size: 

                4545    1857      73
 
BamHI G'GATC_C

Cuts at:   3805    4172    3805 
   Size:        367    6108
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3311    3796    5251     618 
   Size:        325     217     627    1524     485    1455    1842

  Fragments arranged by size: 

                1842    1524    1455     627     485     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2064    2670    3794    1391 
   Size:        673     606    1124    4072

  Fragments arranged by size: 

                4072    1124     673     606
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3561     928 
   Size:        971    1662    3842

  Fragments arranged by size: 

                3842    1662     971
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3581    3615    4666    4700    1903 
   Size:         34      50      34    1560      34    1051      34    3678

  Fragments arranged by size: 

                3678    1560    1051      50      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   4367    5577    4367 
   Size:       1210    5265
 
BclI T'GATC_A

Cuts at:   1548    2016    1548 
   Size:        468    6007
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5086     136 
   Size:        107     122      71     631     605    3414    1525

  Fragments arranged by size: 

                3414    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6470    6470 
   Size:       6475
 
BlpI GC'TnA_GC

Cuts at:   2247    3384    3892    2247 
   Size:       1137     508    4830

  Fragments arranged by size: 

                4830    1137     508
 
Bme1580I G_kGCm'C

Cuts at:   1790    4536    5692    1790 
   Size:       2746    1156    2573

  Fragments arranged by size: 

                2746    2573    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6475
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2823    3346    3629 
   Size:        416      53      83     399      24    1406     523     283

Cuts at:   3629    4206    5164     442 
   Size:        577     958    1753

  Fragments arranged by size: 

                1753    1406     958     577     523     416     399     283
                  83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6475
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2083    2083 
   Size:       6475
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2078    2342    2799    3732    5135    1239 
   Size:        839     264     457     933    1403    2579

  Fragments arranged by size: 

                2579    1403     933     839     457     264
 
Bpu10I CC'TnA_GC

Cuts at:   3077    3077 
   Size:       6475
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3530    4600    5465    5763    3530 
   Size:       1070     865     298    4242

  Fragments arranged by size: 

                4242    1070     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    5138     882 
   Size:        617    3639    2219

  Fragments arranged by size: 

                3639    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4266     493 
   Size:        711    3062    2702

  Fragments arranged by size: 

                3062    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6469    6469 
   Size:       6475
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4282    4664    5299     275 
   Size:         53      83     186     936    2749     382     635    1451

  Fragments arranged by size: 

                2749    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4908    5306    5580    5727    6138    4908 
   Size:        398     274     147     411    5245

  Fragments arranged by size: 

                5245     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2283    2313    1178 
   Size:         30     165      30     880      30    5340

  Fragments arranged by size: 

                5340     880     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2370    2728    1186 
   Size:       1184     358    4933

  Fragments arranged by size: 

                4933    1184     358
 
BseYI C'CCAG_C

Cuts at:   3021    5678    6174    3021 
   Size:       2657     496    3322

  Fragments arranged by size: 

                3322    2657     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3342    1163 
   Size:        573    1606    4296

  Fragments arranged by size: 

                4296    1606     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3925    4686    5714    1540 
   Size:        275     186    1924     761    1028    2301

  Fragments arranged by size: 

                2301    1924    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2623    3377    4536    4621    5692    2623 
   Size:        754    1159      85    1071    3406

  Fragments arranged by size: 

                3406    1159    1071     754      85
 
BsmI GAATG_Cn'

Cuts at:   2928    3986    4079    2928 
   Size:       1058      93    5324

  Fragments arranged by size: 

                5324    1058      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   3059    3059 
   Size:       6475
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2064    2623    2670    3377    3794    4536    4621 
   Size:        399     274     559      47     707     417     742      85

Cuts at:   4621    5692    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     707     559     417     399     274
                  85      47
 
BspEI T'CCGG_A

Cuts at:   6138    6138 
   Size:       6475
 
BspHI T'CATG_A

Cuts at:   2938    4364    6094    2938 
   Size:       1426    1730    3319

  Fragments arranged by size: 

                3319    1730    1426
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2964    3830     844 
   Size:       2120     866    3489

  Fragments arranged by size: 

                3489    2120     866
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4362    1394 
   Size:        429    2539    3507

  Fragments arranged by size: 

                3507    2539     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4970    5144      65 
   Size:       1183    3722     174    1396

  Fragments arranged by size: 

                3722    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5119    5306    1700 
   Size:        110    3309     187    2869

  Fragments arranged by size: 

                3309    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6475
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6475
 
BssSI C'ACGA_G

Cuts at:   4535    5547    4535 
   Size:       1012    5463
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2627     794 
   Size:       1833    4642
 
BstBI TT'CG_AA

Cuts at:   2650    3035    3470    5279    2650 
   Size:        385     435    1809    3846

  Fragments arranged by size: 

                3846    1809     435     385
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6475
 
BstYI r'GATC_y

Cuts at:   1972    3447    3681    3805    4172    4557    4574    6026    6112 
   Size:       1475     234     124     367     385      17    1452      86

Cuts at:   6112    6124    6470    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1475    1452     385     367     346     234     124
                  86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2081    2093    2081 
   Size:         12    6463
 
BtgI C'CryG_G

Cuts at:    513    1065    2109     513 
   Size:        552    1044    4879

  Fragments arranged by size: 

                4879    1044     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2342    3560     487 
   Size:        683      56    1116    1218    3402

  Fragments arranged by size: 

                3402    1218    1116     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2421    3018    3604    3991    4785    4805    2421 
   Size:        597     586     387     794      20    4091

  Fragments arranged by size: 

                4091     794     597     586     387      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6475
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6440
 
DraI TTT'AAA

Cuts at:   3779    3779 
   Size:       6475
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2123    5482     809 
   Size:        507     807    3359    1802

  Fragments arranged by size: 

                3359    1802     807     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3922    4811    6440 
   Size:         54    1425      50     275      18    2092     889    1629

Cuts at:   6440       8 
   Size:         43

  Fragments arranged by size: 

                2092    1629    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3922    1537 
   Size:        275    2110    4090

  Fragments arranged by size: 

                4090    2110     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3519    4405    1353 
   Size:       2166     886    3423

  Fragments arranged by size: 

                3423    2166     886
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2752    5062    5436    5582     387 
   Size:       1064     138    1163    2310     374     146    1280

  Fragments arranged by size: 

                2310    1280    1163    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2078    2237    2342    2799    3509    3515    3732 
   Size:        393     446     159     105     457     710       6     217

Cuts at:   3732    4538    5135    5922    6190    1239 
   Size:        806     597     787     268    1524

  Fragments arranged by size: 

                1524     806     787     710     597     457     446     393
                 268     217     159     105       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2306    2385    2731    2306 
   Size:         79     346    6050

  Fragments arranged by size: 

                6050     346      79
 
EcoRI G'AATT_C

Cuts at:   3037    3784    4162    3037 
   Size:        747     378    5350

  Fragments arranged by size: 

                5350     747     378
 
FspI TGC'GCA

Cuts at:   4981    4981 
   Size:       6475
 
HaeII r_GCGC'y

Cuts at:   1678    3566    5622    1678 
   Size:       1888    2056    2531

  Fragments arranged by size: 

                2531    2056    1888
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2021    2053    2503    2535    2552 
   Size:         32     135      32     449      32     450      32      17

Cuts at:   2552    2584    3102    3134    5116    5148    5190    5222    1373 
   Size:         32     518      32    1982      32      42      32    2626

  Fragments arranged by size: 

                2626    1982     518     450     449     135      42      32
                  32      32      32      32      32      32      32      17
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3819    4065     669 
   Size:        988     281    1881     246    3079

  Fragments arranged by size: 

                3079    1881     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2441    3230     748 
   Size:       1693     789    3993

  Fragments arranged by size: 

                3993    1693     789
 
HpaI GTT'AAC

Cuts at:   4065    4065 
   Size:       6475
 
KpnI G_GTAC'C

Cuts at:   3315    3800    3315 
   Size:        485    5990
 
MfeI C'AATT_G

Cuts at:   2138    4074    2138 
   Size:       1936    4539
 
MluI A'CGCG_T

Cuts at:   6351    6351 
   Size:       6475
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2510    5589    5773    1305 
   Size:       1205    3079     184    2007

  Fragments arranged by size: 

                3079    2007    1205     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4653

  Fragments arranged by size: 

                4653    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3141    4433    4792    4951     518 
   Size:        648    1975    1292     359     159    2042

  Fragments arranged by size: 

                2042    1975    1292     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3388    3891    4568    5716    5961    1606 
   Size:       1782     503     677    1148     245    2120

  Fragments arranged by size: 

                2120    1782    1148     677     503     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5923
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6475
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6475
 
NotI GC'GGCC_GC

Cuts at:   3922    3922 
   Size:       6475
 
NspI r_CATG'y

Cuts at:   1132    1229    3833    5378    1132 
   Size:         97    2604    1545    2229

  Fragments arranged by size: 

                2604    2229    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6475
 
PciI A'CATG_T

Cuts at:   5374    5374 
   Size:       6475
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2492    2529    2822    2843    3691    1108 
   Size:       1384      37     293      21     848    3892

  Fragments arranged by size: 

                3892    1384     848     293      37      21
 
PfoI T'CCnGG_A

Cuts at:   3684    3748    3684 
   Size:         64    6411
 
PmeI GTTT'AAAC

Cuts at:   3779    3779 
   Size:       6475
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4544    4576    6057    6089    4544 
   Size:         32    1481      32    4930

  Fragments arranged by size: 

                4930    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6475
 
PsiI TTA'TAA

Cuts at:   4045    4045 
   Size:       6475
 
PstI C_TGCA'G

Cuts at:    830    3195    3827     830 
   Size:       2365     632    3478

  Fragments arranged by size: 

                3478    2365     632
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6475
 
PvuII CAG'CTG

Cuts at:   3388    3388 
   Size:       6475
 
SalI G'TCGA_C

Cuts at:   1936    3817    1936 
   Size:       1881    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   3519    3519 
   Size:       6475
 
SbfI CC_TGCA'GG

Cuts at:   3827    3827 
   Size:       6475
 
ScaI AGT'ACT

Cuts at:   4723    6435    4723 
   Size:       1712    4763
 
SfcI C'TryA_G

Cuts at:    826    1045    3191    3823    4958    5639    5830     826 
   Size:        219    2146     632    1135     681     191    1471

  Fragments arranged by size: 

                2146    1471    1135     681     632     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6475
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6475
 
SmaI CCC'GGG

Cuts at:   1872    3802    1872 
   Size:       1930    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3509    4579    5480    5742     820 
   Size:        197     926    1566    1070     901     262    1553

  Fragments arranged by size: 

                1566    1553    1070     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4266     493 
   Size:       3773    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6475
 
SphI G_CATG'C

Cuts at:   1132    3833    1132 
   Size:       2701    3774
 
SspI AAT'ATT

Cuts at:      5      52    4399       5 
   Size:         47    4347    2081

  Fragments arranged by size: 

                4347    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6475
 
StyI C'CwwG_G

Cuts at:    513    1065    2997    3273    3914     513 
   Size:        552    1932     276     641    3074

  Fragments arranged by size: 

                3074    1932     641     552     276
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3181    4502    4519    4672    4857    5302     325 
   Size:       1255    1601    1321      17     153     185     445    1498

  Fragments arranged by size: 

                1601    1498    1321    1255     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2423    2604    4721 
   Size:        274      80      33      51    1443     446     181    2117

Cuts at:   4721    6433      96 
   Size:       1712     138

  Fragments arranged by size: 

                2117    1712    1443     446     274     181     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6435    6467    6435 
   Size:         32    6443
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6475
 
XbaI T'CTAG_A

Cuts at:   3811    3811 
   Size:       6475
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2995    2995 
   Size:       6475
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6475
 
XmaI C'CCGG_G

Cuts at:   1870    3800    1870 
   Size:       1930    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2154    3528    4604    2154 
   Size:       1374    1076    4025

  Fragments arranged by size: 

                4025    1374    1076
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4283     276 
   Size:         53      83     186     936    2749    2468

  Fragments arranged by size: 

                2749    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI     PstI
     PvuI    PvuII     SalI     SapI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI    BsiWI    BstXI  BstZ17I   DraIII  EcoICRI EcoO109I
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PmlI    PpuMI   PspOMI    PspXI     PsrI
    RsrII     SacI    SacII    SanDI    SexAI     SfiI     SfoI     SrfI
     SwaI
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