(Circular) (Six-base) MAPSORT of: pFN21AA0743.seq Check: 8556 from: 1 to: 7888 pFN21AA0743 7888 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4049 4453 4049 Size: 404 7484 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5698 278 Size: 53 83 186 936 4162 2468 Fragments arranged by size: 4162 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2400 4733 5231 1937 Size: 463 2333 498 4594 Fragments arranged by size: 4594 2333 498 463 Acc65I G'GTAC_C Cuts at: 2511 5209 2511 Size: 2698 5190 AclI AA'CG_TT Cuts at: 1563 6015 6388 1563 Size: 4452 373 3063 Fragments arranged by size: 4452 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3482 3530 4234 5951 7335 7603 1632 Size: 1850 48 704 1717 1384 268 1917 Fragments arranged by size: 1917 1850 1717 1384 704 268 48 AflII C'TTAA_G Cuts at: 820 1017 3248 820 Size: 197 2231 5460 Fragments arranged by size: 5460 2231 197 AflIII A'CryG_T Cuts at: 1203 2622 6787 7764 1203 Size: 1419 4165 977 1327 Fragments arranged by size: 4165 1419 1327 977 AgeI A'CCGG_T Cuts at: 6719 6719 Size: 7888 AhdI GACnn_n'nnGTC Cuts at: 5050 6617 5050 Size: 1567 6321 AleI CACnn'nnGTG Cuts at: 1166 3806 4259 1166 Size: 2640 453 4795 Fragments arranged by size: 4795 2640 453 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2796 1184 Size: 1612 6276 AlwNI CAG_nnn'CTG Cuts at: 5631 7203 5631 Size: 1572 6316 ApaLI G'TGCA_C Cuts at: 5945 7101 5945 Size: 1156 6732 ApoI r'AATT_y Cuts at: 1493 5197 5423 5575 7684 1493 Size: 3704 226 152 2109 1697 Fragments arranged by size: 3704 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6442 160 Size: 872 5410 1606 Fragments arranged by size: 5410 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7888 AvaI C'yCGr_G Cuts at: 1870 1943 3765 3918 5213 1870 Size: 73 1822 153 1295 4545 Fragments arranged by size: 4545 1822 1295 153 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3273 3306 3273 Size: 33 7855 BamHI G'GATC_C Cuts at: 5218 5585 5218 Size: 367 7521 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2462 2511 2897 3830 5209 Size: 325 217 627 675 49 386 933 1379 Cuts at: 5209 6664 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1379 933 675 627 386 325 217 49 BanII G_rGCy'C Cuts at: 1391 2015 2294 2325 2459 4593 5138 5207 1391 Size: 624 279 31 134 2134 545 69 4072 Fragments arranged by size: 4072 2134 624 545 279 134 69 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4345 4866 928 Size: 971 2446 521 3950 Fragments arranged by size: 3950 2446 971 521 BbvCI CC'TCA_GC Cuts at: 3276 3276 Size: 7888 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3203 3237 4699 4733 6079 Size: 34 50 34 1182 34 1462 34 1346 Cuts at: 6079 6113 1903 Size: 34 3678 Fragments arranged by size: 3678 1462 1346 1182 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2897 5780 6990 2897 Size: 2883 1210 3795 Fragments arranged by size: 3795 2883 1210 BclI T'GATC_A Cuts at: 1548 3705 1548 Size: 2157 5731 BfrBI ATG'CAT Cuts at: 2992 2992 Size: 7888 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6499 136 Size: 107 122 71 631 605 4827 1525 Fragments arranged by size: 4827 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7883 7883 Size: 7888 BlpI GC'TnA_GC Cuts at: 5305 5305 Size: 7888 Bme1580I G_kGCm'C Cuts at: 1790 5949 7105 1790 Size: 4159 1156 2573 Fragments arranged by size: 4159 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7888 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2917 4026 5619 Size: 416 53 83 399 24 1500 1109 1593 Cuts at: 5619 6577 442 Size: 958 1753 Fragments arranged by size: 1753 1593 1500 1109 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7888 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 3049 3113 3142 3355 4281 4301 6548 Size: 917 893 64 29 213 926 20 2247 Cuts at: 6548 1239 Size: 2579 Fragments arranged by size: 2579 2247 926 917 893 213 64 29 20 Bpu10I CC'TnA_GC Cuts at: 2942 3276 3530 2942 Size: 334 254 7300 Fragments arranged by size: 7300 334 254 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6013 6878 7176 6013 Size: 865 298 6725 Fragments arranged by size: 6725 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2234 3167 3288 6551 882 Size: 617 735 933 121 3263 2219 Fragments arranged by size: 3263 2219 933 735 617 121 BsaAI yAC'GTr Cuts at: 493 1204 2707 4158 5185 5679 493 Size: 711 1503 1451 1027 494 2702 Fragments arranged by size: 2702 1503 1451 1027 711 494 BsaBI GATnn'nnATC Cuts at: 2177 7882 2177 Size: 5705 2183 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3964 4564 5695 6077 Size: 53 83 186 936 2431 600 1131 382 Cuts at: 6077 6712 275 Size: 635 1451 Fragments arranged by size: 2431 1451 1131 936 635 600 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2900 6321 6719 6993 7140 7551 2900 Size: 3421 398 274 147 411 3237 Fragments arranged by size: 3421 3237 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3140 3170 1178 Size: 30 165 30 1737 30 5896 Fragments arranged by size: 5896 1737 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2411 4403 1186 Size: 1225 1992 4671 Fragments arranged by size: 4671 1992 1225 BseYI C'CCAG_C Cuts at: 2008 3030 4217 7091 7587 2008 Size: 1022 1187 2874 496 2309 Fragments arranged by size: 2874 2309 1187 1022 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2875 1163 Size: 573 1139 6176 Fragments arranged by size: 6176 1139 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5338 6099 7127 1540 Size: 275 186 3337 761 1028 2301 Fragments arranged by size: 3337 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4297 5138 5949 6034 7105 4297 Size: 841 811 85 1071 5080 Fragments arranged by size: 5080 1071 841 811 85 BsmI GAATG_Cn' Cuts at: 2782 4803 5399 5492 2782 Size: 2021 596 93 5178 Fragments arranged by size: 5178 2021 596 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5063 5063 Size: 7888 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2294 2325 2459 4297 4593 5138 Size: 399 225 279 31 134 1838 296 545 Cuts at: 5138 5207 5949 6034 7105 1391 Size: 69 742 85 1071 2174 Fragments arranged by size: 2174 1838 1071 742 545 399 296 279 225 134 85 69 31 BspEI T'CCGG_A Cuts at: 7551 7551 Size: 7888 BspHI T'CATG_A Cuts at: 3336 5115 5777 7507 3336 Size: 1779 662 1730 3717 Fragments arranged by size: 3717 1779 1730 662 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2927 4049 4453 5243 844 Size: 2083 1122 404 790 3489 Fragments arranged by size: 3489 2083 1122 790 404 BsrBI CCG'CTC Cuts at: 1394 1823 4002 5145 5775 1394 Size: 429 2179 1143 630 3507 Fragments arranged by size: 3507 2179 1143 630 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3637 3639 4147 6383 6557 65 Size: 1183 2389 2 508 2236 174 1396 Fragments arranged by size: 2389 2236 1396 1183 508 174 2 BsrFI r'CCGG_y Cuts at: 1700 1810 2894 4503 6532 6719 1700 Size: 110 1084 1609 2029 187 2869 Fragments arranged by size: 2869 2029 1609 1084 187 110 BsrGI T'GTAC_A Cuts at: 96 2961 3888 5025 96 Size: 2865 927 1137 2959 Fragments arranged by size: 2959 2865 1137 927 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7888 BssSI C'ACGA_G Cuts at: 3908 5948 6960 3908 Size: 2040 1012 4836 Fragments arranged by size: 4836 2040 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7888 BstBI TT'CG_AA Cuts at: 6692 6692 Size: 7888 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7888 BstXI CCAn_nnnn'nTGG Cuts at: 3841 4586 3841 Size: 745 7143 BstYI r'GATC_y Cuts at: 1972 2601 5218 5585 5970 5987 7439 7525 7537 Size: 629 2617 367 385 17 1452 86 12 Cuts at: 7537 7883 1972 Size: 346 1977 Fragments arranged by size: 2617 1977 1452 629 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2412 2982 3258 4242 4896 513 Size: 552 1347 570 276 984 654 3505 Fragments arranged by size: 3505 1347 984 654 570 552 276 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3475 487 Size: 683 56 2249 4900 Fragments arranged by size: 4900 2249 683 56 BtsI GCAGTG_nn' Cuts at: 2828 5404 6198 6218 2828 Size: 2576 794 20 4498 Fragments arranged by size: 4498 2576 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7888 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3891 3926 529 Size: 35 3327 35 4491 Fragments arranged by size: 4491 3327 35 35 DraI TTT'AAA Cuts at: 5192 5192 Size: 7888 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6895 809 Size: 507 5579 1802 Fragments arranged by size: 5579 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2214 3382 4199 Size: 54 1425 50 275 18 384 1168 817 Cuts at: 4199 4475 5146 5335 6224 7853 8 Size: 276 671 189 889 1629 43 Fragments arranged by size: 1629 1425 1168 889 817 671 384 276 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5335 1537 Size: 275 3523 4090 Fragments arranged by size: 4090 3523 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2345 3549 3575 3617 3996 4574 5135 5818 Size: 992 1204 26 42 379 578 561 683 Cuts at: 5818 1353 Size: 3423 Fragments arranged by size: 3423 1204 992 683 578 561 379 42 26 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5083 6475 6849 6995 387 Size: 1064 138 3494 1392 374 146 1280 Fragments arranged by size: 3494 1392 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5136 5136 Size: 7888 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 3049 3113 3142 3355 3482 3530 Size: 393 524 893 64 29 213 127 48 Cuts at: 3530 4234 4281 4301 5951 6548 7335 7603 1239 Size: 704 47 20 1650 597 787 268 1524 Fragments arranged by size: 1650 1524 893 787 704 597 524 393 268 213 127 64 48 47 29 20 EcoNI CCTnn'n_nnAGG Cuts at: 2102 2923 2102 Size: 821 7067 EcoO109I rG'GnC_Cy Cuts at: 4028 4926 5052 4028 Size: 898 126 6864 Fragments arranged by size: 6864 898 126 EcoRI G'AATT_C Cuts at: 5197 5575 5197 Size: 378 7510 FspI TGC'GCA Cuts at: 6394 6394 Size: 7888 HaeII r_GCGC'y Cuts at: 1678 4155 7035 1678 Size: 2477 2880 2531 Fragments arranged by size: 2880 2531 2477 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2441 2473 2525 2557 2996 Size: 32 135 32 869 32 52 32 439 Cuts at: 2996 3028 5036 5068 6529 6561 6603 6635 1373 Size: 32 2008 32 1461 32 42 32 2626 Fragments arranged by size: 2626 2008 1461 869 439 135 52 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3127 3255 4372 4512 5232 5478 Size: 988 281 1189 128 1117 140 720 246 Cuts at: 5478 669 Size: 3079 Fragments arranged by size: 3079 1189 1117 988 720 281 246 140 128 HindIII A'AGCT_T Cuts at: 748 748 Size: 7888 HpaI GTT'AAC Cuts at: 3127 3255 4512 5478 3127 Size: 128 1257 966 5537 Fragments arranged by size: 5537 1257 966 128 KpnI G_GTAC'C Cuts at: 2515 5213 2515 Size: 2698 5190 MfeI C'AATT_G Cuts at: 5487 5487 Size: 7888 MluI A'CGCG_T Cuts at: 7764 7764 Size: 7888 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4654 7002 7186 1305 Size: 3349 2348 184 2007 Fragments arranged by size: 3349 2348 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2216 3384 4201 4477 10 Size: 54 1425 343 384 1168 817 276 3421 Fragments arranged by size: 3421 1425 1168 817 384 343 276 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 2471 2963 2981 2987 3560 3806 Size: 648 1005 300 492 18 6 573 246 Cuts at: 3806 4259 5114 5846 6205 6364 518 Size: 453 855 732 359 159 2042 Fragments arranged by size: 2042 1005 855 732 648 573 492 453 359 300 246 159 18 6 MspA1I CmG'CkG Cuts at: 1606 2054 2761 5304 5981 7129 7374 1606 Size: 448 707 2543 677 1148 245 2120 Fragments arranged by size: 2543 2120 1148 707 677 448 245 NaeI GCC'GGC Cuts at: 4505 4505 Size: 7888 NcoI C'CATG_G Cuts at: 513 1065 2982 513 Size: 552 1917 5419 Fragments arranged by size: 5419 1917 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7888 NgoMIV G'CCGG_C Cuts at: 4503 4503 Size: 7888 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7888 NotI GC'GGCC_GC Cuts at: 5335 5335 Size: 7888 NsiI A_TGCA'T Cuts at: 2994 2994 Size: 7888 NspI r_CATG'y Cuts at: 1132 1229 2626 2962 3889 4081 5246 6791 1132 Size: 97 1397 336 927 192 1165 1545 2229 Fragments arranged by size: 2229 1545 1397 1165 927 336 192 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7888 PciI A'CATG_T Cuts at: 2622 6787 2622 Size: 4165 3723 PflMI CCAn_nnn'nTGG Cuts at: 1108 4304 1108 Size: 3196 4692 PfoI T'CCnGG_A Cuts at: 2604 4297 4941 2604 Size: 1693 644 5551 Fragments arranged by size: 5551 1693 644 PmeI GTTT'AAAC Cuts at: 5192 5192 Size: 7888 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5957 5989 7470 7502 5957 Size: 32 1481 32 6343 Fragments arranged by size: 6343 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4028 5052 4028 Size: 1024 6864 PshAI GACnn'nnGTC Cuts at: 1613 2130 1613 Size: 517 7371 PsiI TTA'TAA Cuts at: 2115 5458 2115 Size: 3343 4545 PstI C_TGCA'G Cuts at: 830 3393 4464 5240 830 Size: 2563 1071 776 3478 Fragments arranged by size: 3478 2563 1071 776 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7888 PvuII CAG'CTG Cuts at: 2761 2761 Size: 7888 SacI G_AGCT'C Cuts at: 5138 5138 Size: 7888 SalI G'TCGA_C Cuts at: 1936 5230 1936 Size: 3294 4594 SapI GCTCTTCn'nnn_ Cuts at: 3996 5135 3996 Size: 1139 6749 SbfI CC_TGCA'GG Cuts at: 4464 5240 4464 Size: 776 7112 ScaI AGT'ACT Cuts at: 4614 6136 7848 4614 Size: 1522 1712 4654 Fragments arranged by size: 4654 1712 1522 SfcI C'TryA_G Cuts at: 826 1045 3389 4460 4497 4794 5236 6371 7052 Size: 219 2344 1071 37 297 442 1135 681 Cuts at: 7052 7243 826 Size: 191 1471 Fragments arranged by size: 2344 1471 1135 1071 681 442 297 219 191 37 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7888 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7888 SmaI CCC'GGG Cuts at: 1872 5215 1872 Size: 3343 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3248 3918 5992 6893 7155 820 Size: 197 926 1305 670 2074 901 262 1553 Fragments arranged by size: 2074 1553 1305 926 901 670 262 197 SnaBI TAC'GTA Cuts at: 493 4158 5679 493 Size: 3665 1521 2702 Fragments arranged by size: 3665 2702 1521 SpeI A'CTAG_T Cuts at: 152 152 Size: 7888 SphI G_CATG'C Cuts at: 1132 4081 5246 1132 Size: 2949 1165 3774 Fragments arranged by size: 3774 2949 1165 SspI AAT'ATT Cuts at: 5 52 4645 5812 5 Size: 47 4593 1167 2081 Fragments arranged by size: 4593 2081 1167 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7888 StyI C'CwwG_G Cuts at: 513 1065 2982 3880 4573 5327 513 Size: 552 1917 898 693 754 3074 Fragments arranged by size: 3074 1917 898 754 693 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5915 5932 6085 6270 6715 325 Size: 1255 4335 17 153 185 445 1498 Fragments arranged by size: 4335 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2436 2961 3888 Size: 274 80 33 51 1443 459 525 927 Cuts at: 3888 4612 4883 5025 5031 6134 7846 96 Size: 724 271 142 6 1103 1712 138 Fragments arranged by size: 1712 1443 1103 927 724 525 459 274 271 142 138 80 51 33 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4162 4194 7848 7880 4162 Size: 32 3654 32 4170 Fragments arranged by size: 4170 3654 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3500 1316 Size: 2184 5704 XbaI T'CTAG_A Cuts at: 5224 5224 Size: 7888 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3038 4462 4759 3038 Size: 1424 297 6167 Fragments arranged by size: 6167 1424 297 XhoI C'TCGA_G Cuts at: 1943 3918 1943 Size: 1975 5913 XmaI C'CCGG_G Cuts at: 1870 5213 1870 Size: 3343 4545 XmnI GAAnn'nnTTC Cuts at: 2876 6017 2876 Size: 3141 4747 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5696 276 Size: 53 83 186 936 4162 2468 Fragments arranged by size: 4162 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI